Chemical Compatibility, continued

Chemical
Acetic acid, glacial 1 Formaldehyde Nitrogen
Acetone 2, 3 Formic acid (50%) 1, 3 Ozone (10 ppm in water)
User Guide Acetonitrile 3 Freon® (TF or PCA) solvent Paraldehyde
Ammonium hydroxide 1, 3 Gasoline 3 Perchloroethylene 1, 3
Ammonium sulfate (saturated) 2, 3 Glycerine (glycerol) Petroleum based oils 3
Nonsterile 33 mm Millex® Amyl acetate 3 Helium Petroleum ether 3

Syringe Filters Amyl alcohol

Benzyl alcohol
Hexane 3
Hydrochloric acid 1
Phenol (10%)
Potassium hydroxide 1,3
Boric acid Hydrofluoric acid 1 Pyridine 1, 2, 3
Millex®-LG, LCR, GV, HV, Butyl alcohol Hydrogen Silicone oils
GN, HN, GP, HP, Cellosolve® (ethyl) solvent 3 Hydrogen peroxide (3%) Sodium carbonate (aqueous solution) 1, 3
Chloroform 1, 3 Hypo (sodium thiosulfate) 3 Sodium chloride (2 M)
Cyclohexane 3 Isobutyl alcohol Sodium hydroxide (3 N) 1, 3
For laboratory use only Cyclohexanone 2, 3 Isopropyl acetate 3 Sulfuric acid (3 N) 1
Dimethylacetamide 2, 3 Isopropyl alcohol Tetrahydrofuran 3
Dimethylformamide 2, 3 Kerosene 3 Toluene 3
Dimethylsulfoxide 2, 3 Lactic acid (50%) 1, 2, 3 Trichloroacetic acid (aqueous solution) 1, 3
Dioxane 3 Methyl alcohol Trichloroethane 1, 3

Introduction Ethers 3
Ethyl acetate 3
Methylene chloride 1, 3
Methyl ethyl ketone 2, 3
Trichloroethylene 1, 3
Trifluoroacetic acid 1, 3
This document provides chemical compatibility information, operating Ethyl alcohol Methyl isobutyl ketone 2, 3 Urea (8 M) 3
steps, and specifications for the nonsterile 33 millimeter (mm) Ethylene glycol Mineral spirits 3 Xylene 3
Millex® syringe filter with male Luer-slip outlet. This syringe filter is 1
Not compatible with GN and HN 2
Not compatible with GV and HV 3
Not compatible with GP and HP
recommended for filtering 10–100 milliliter (mL) volumes to remove
particles prior to instrumentation analysis. The single-use, disposable How to Use 33 mm Millex® Syringe Filters
filter removes particles larger than the membrane’s rated pore size.
WARNINGS:
The Millex® syringe filter consists of a membrane sealed in a ●● The 33 mm Millex® syringe filter is intended for laboratory use only
polypropylene housing. For details on the type of membrane in your and is not a medical device for direct patient care applications.
Millex® syringe filter, refer to the table below.
●● Do not use with syringes smaller than 10 mL because pressures in
Filter Membrane Application excess of the maximum pressure rating may be reached, potentially
causing damage to the filter unit and/or personal injury.
LG 0.20 µm hydrophilic PTFE Filtration of protein-containing solutions,
(polytetrafluoroethylene) and aqueous or organic solutions CAUTIONS:
LCR 0.45 µm hydrophilic PTFE Filtration of protein-containing solutions, ●● Do not use the syringe filter at temperatures above 45 °C (113 °F).
and aqueous or organic solutions ●● Do not use the same 33 mm syringe filter to filter solutions in both
GV 0.22 µm PVDF Filtration of protein-containing solutions, directions.
(polyvinylidene fluoride) and aqueous or mild organic solutions ●● Do not reuse the syringe filter.
HV 0.45 µm PVDF Filtration of protein-containing solutions, ●● Do not use the syringe filter to filter emulsions or suspensions.
and aqueous or mild organic solutions ●● Perform a binding study before use if there is a concern about loss
GN 0.20 µm nylon Filtration of aqueous or organic solutions of analyte (proteins, nucleic acids, active pharmaceuticals) due to
HN 0.45 µm nylon Filtration of aqueous or organic solutions
binding.
GP 0.22 µm PES Filtration of protein-containing solutions, Instructions for Use
(polyethersulfone) and aqueous or mild organic solutions
1 2
HP 0.45 µm PES Filtration of protein-containing solutions,
and aqueous or mild organic solutions

Chemical Compatibility
Millex® syringe filters are compatible with aqueous, mild organic,
and organic solutions. You can use them to filter the agents listed in
the following table. This information was developed from technical
publications, materials suppliers, and laboratory tests, and is believed to
be accurate and reliable. However, because of variability in temperature,
Fill the syringe with the Attach the syringe to the
concentrations, exposure time, and other factors beyond our control that solution to be filtered. 33 mm Millex® syringe filter.
may affect the use of the unit, no warranty is provided or implied with
respect to such information. Agents not listed in the following table should
3 4
be tested with the Millex® syringe filter prior to use.
NOTE: For low extractable HPLC instrumentation analysis applications, it
is recommended that you discard the first 1 mL or rinse with 1 to
2 mL of primary solvent before sample filtration.

Hold the syringe with filter pointing Push the syringe plunger to
up and “top off” by pushing a few deliver the filtered solution.
drops through the filter.
Optional: To purge the syringe filter and
maximize sample throughput, remove the
Millex® filter from the syringe and draw air into
the syringe. Then reattach the Millex® filter,
and push the plunger to force some of the air
through the filter.

3273IL1mac
Specifications
Product Ordering Information
Housing Polypropylene
Membrane This section lists the catalogue numbers for 33 mm nonsterile Millex®
LG, LCR Hydrophilic PTFE syringe filters. You can purchase these products on-line at
GV, HV Hydrophilic Durapore® PVDF www.sigmaaldrich.com/products, or see contact information below.
GN, HN Hydrophilic Nylon Syringe filter Color 50/pk 250/pk 1000/pk
GP, HP Hydrophilic Millipore Express® PES
Millex®-LG, Light Blue SLLG033NS SLLG033NB SLLG033NK
Dimensions PTFE 0.20 µm
Inlet to outlet 27 mm (1.1 in.)
Millex®-LCR, Light Blue SLCR033NS SLCR033NB SLCR033NK
Diameter 33 mm (1.3 in.)
PTFE 0.45 µm
Filtration surface area 4.5 cm2 (0.7 in.2)
Millex®-GV, Yellow SLGV033NS SLGV033NB SLGV033NK
Pore size
PVDF, 0.22 µm
GV, GP 0.22 µm
LG, GN 0.20 µm Millex®-HV Yellow SLHV033NS SLHV033NB SLHV033NK
LCR, HV, HN, HP 0.45 µm PVDF, 0.45 µm

Temperature limit 45 °C (113 °F) Millex®-GN, Purple SLGN033NS SLGN033NB SLGN033NK

Connections Female Luer-Lok™ inlet, male Luer-slip Nylon 0.20 µm
outlet Millex®-HN, Purple SLHN033NS SLHN033NB SLHN033NK
Pressure limit at 21 °C 8.6 bar (125 psi) differential Nylon 0.45 µm
Filtration volume 10–100 mL Millex®-GP, Green SLGP033NS SLGP033NB SLGP033NK
Hold-up volume ≤ 80 µL after air purge at pressure that PES, 0.22 µm
exceeds bubble point of the membrane Millex®-HP, Green SLHP033NS SLHP033NB SLHP033NK
PES, 0.45 µm
Performance
Membrane Typical Water Flow Rate Notice
Type at 21 °C and 2.1 bar (30 psi) Bubble point We provide information and advice to our customers on application
LG 185 mL/min ≥ 2.8 bar (≥ 41 psi) technologies and regulatory matters to the best of our knowledge and
LCR 438 mL/min ≥ 1.5 bar (≥ 22 psi) ability, but without obligation or liability. Existing laws and regulations are
to be observed in all cases by our customers. This also applies in respect
GV 94 mL/min ≥ 3.4 bar (≥ 50 psi) to any rights of third parties. Our information and advice do not relieve
HV 423 mL/min ≥ 1.5 bar (≥ 22 psi) our customers of their own responsibility for checking the suitability of
our products for the envisaged purpose.
GN 75 mL/min ≥ 3.0 bar (≥ 43 psi)
HN 169 mL/min ≥ 2.2 bar (≥ 32 psi) Contact Information
GP 240 mL/min ≥ 4.3 bar (≥ 63 psi) For the location of the office nearest you, go to
www.sigmaaldrich.com/offices.
HP 480 mL/min ≥ 2.0 bar (≥ 29 psi)

HPLC Certification Technical Assistance

Visit the tech service page on our web site at
Millex®-LG, LCR, GN, and HN syringe filters are tested for UV-absorbing
www.sigmaaldrich.com/techservice.
extractables. HPLC analysis of 1 mL samples of both acetonitrile and
water collected after discarding the first 1 mL of solvent showed no peaks
greater in intensity than 0.004 AUFS (after the column frontal volume) at
Standard Warranty
either 214 nm or 254 nm. Representative samples of all lots manufactured The applicable warranty for the products listed in this publication may be
are tested. found at www.sigmaaldrich.com/terms (“Conditions of Sale”).

The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma
in the U.S. and Canada.

The initial M, Millipore, Durapore, Millipore Express, and Millex are trademarks of Merck KGaA,
Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective
owners. Detailed information on trademarks is available via publicly accessible resources.
© 2019 Merck KGaA, Darmstadt, Germany and/or its affiliates. All rights reserved.
PR05488, Rev. 02/19