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This meant I could do two repeats at each temperature in the time it would take to just find the
amount of DCPIP needed at one temperature. With this in mind I would suggest that this experiment
is in adequate to measure vitamin c and other methods should be employed. Vit-C prevent the
oxidation of cellular components by the effect of free radicals, peroxides, superoxides etc. These
results match with that of published data for the expected amount of vitamin c in fruits. Share to
Twitter Share to Facebook Share to Pinterest. These sheets could even be used as a short progress
test with students too. Ascorbic acid is toxic to viruses, bacteria, and many types of cultured cells,
because of its prooxidant activity. In considering this information, I would agree with it and I
believe my results to be correct, as they appear to make sense. Get this resource as part of a bundle
and save up to 36% A bundle is a package of resources grouped together to teach a particular topic,
or a series of lessons, in one place. The state (the colour) of decolourisation was supposed to be the
same in both samples. Our range of laboratory equipment includes Lab Equipments, Lab
Spectrophotometer, Laboratory Autoclave, Friability Test Apparatus, Laboratory Refractometer,
Polarimeter Instrument, Laboratory Colorimeter, pH Meter, Microscope etc. Now these chemicals are
sometimes warranted, but also they will have some consequences to the environment or ecology.
Many uses for vitamin C have been proposed, but few have been found to be bene?cial in scienti?c
studies. Also, to check whether or not the maturity state has influence on the change of vitamin C
concentration, the newly bought and old lemon were used. Upload Read for free FAQ and support
Language (EN) Sign in Skip carousel Carousel Previous Carousel Next What is Scribd. It is thought
that ascorbic acid reacts with these free radicals preventing them from causing damage to cells. It is
not soulble in chloroform, solvent ether and light petroleum. The scientific terms used are presented
to a high level of clearness and understanding of the topic. I kept this colour as the standard colour
to indicate when the reaction had finished, and managed to control the amount of DCPIP very
precisely due to the burette which dripped slowly into the beaker. In the process of making
commercial fruit juices, fruits are transported through long distances from its origin country has
cause the freshness of the fruit to deteriorate and hence the significant loss of vitamin C. This leads
me to believe that either it is anomalous OR that the number of vitamin C molecules slightly
increases before decreasing again. The black and white copies could be coloured in by students to aid
memory of the heart structure. Tomatoes, blackcurrants, strawberries, blueberries, cranberries,
pineapples, kiwi fruit, papaya, mangoes, guavas and citrus fruits are also excellent sources of vitamin
C. This is because the various enzymes are so powerful in their ability to trigger specific chemical
reactions to take place rapidly that only a gram or two or even a few milligrams may be needed in the
body. Ascorbic acid is also important biologically as it is used as a cofactor in enzymatic reactions
(no less than 8 reactions have been identified). The first lesson was spent planning the practical and
the second one completing it. By using a knife to cut, the amount of vitamin C dissolved in the
distilled water is inaccurate and frequently less than the theoretical amount as parts of the vitamin C
tablet is crushed into powder when force is applied on the tablet. Analysing the vitamin C content in
different fruit juices. Studies have related low vitamin C levels to increased risk for infection. It is
difficult to identify how old fruit is due to the manner in which it is transported from its country of
origin.
Join our team of reviewers and help other students learn. The worksheets are provided in both an A4
and A3 format. Vitamin C deficiency also results in sluggish hormonal function of adrenal gland and
gonads. The more vitamin C, the more DCPIP can be decolourized. Comprehensive corresponding
answer sheets for the teacher. The amount of Vitamin c in the samples that I have measured was
67.0%, 64.8% and 65.0% respectively of the published data for the quantity of vitamin c, (as shown
below). This speeded things up and I kept my method the same as my preliminary. No changes were
made to the content of the solution. A person's diet must provide all the necessary vitamins.
Therefore, one must obtain vitamins from a food on a daily basis. BiochemNetworks. Ethereal
theme. Powered by Blogger. Join our team of reviewers and help other students learn. The conclusion
is adequate and the use of comparison against published values is good. These old agents can react
with new ones, changing their overall concentration. Measuring Vitamin C content in different
substances. WS2 Food chains and energy transfer between trophic levels. Excess vitamin C leaves
the body through the urine. We then took the mixture and filtered it through filter paper into a
beaker. This resource is specified for biology edexcel SNAB new specification but can also be used
for other exam boards. Vitamin C increases body resistance, what is essential to. Vitamin C also
serves with them in bringing about these reactions. The content of vitamin C of each type of fruit
juice can be determined more accurately and may be closer to the theoretical value. Over time and
much wear and tear, this compromised exterior will wear down and a pinhole will result. I predict
that when I plot a graph of temperature against volume of DCPIP used, I will get a straight-line,
indicating direct correlation between the two. The worksheets will help students learn the equations
for respiration, it’s role in living things and also familiarise themselves with key words related to the
topic. This was the first time the RDAs had taken into account the importance of environment and
lifestyle factors in establishing the need for a vitam. All forms of cooking reduce vitamin C levels in
foods. In reality this practical does not always generate the expected results due to sources of error
and time constraints to set up, hence why I have put this investigation pack together. It appears that
vitamin C is involved at every step in the manufacture of collagen.
Ascorbic acid (vitamin c) is freely soluble in water; sparingly soluble in alcohol; insolu ble in chlor
oform, in ether, and in benzene. If starch is placed in the solution the iodine and triiodide molecules
will react with starch to form a blue-black complex. WS1 An introduction to respiration and uses of
energy in the body. Define the terms which describe the characteristics of living organisms. A
completed answer sheet is provided for the teacher. Antioxidants are nutrients that block some of the
damage caused by free radicals, which are by-products that result when our bodies transform food
into energy. If starch is placed in the solution the iodine and triiodide molecules will react with
starch to form a blue-black complex. Ascorbic acid functions as an anti-oxidant in living organisms
protecting the body against the effect of oxidative stress. Thus, the end point of titration is not a
colourless solution but a pink solution in which the colour remains for 10 seconds or more. It is this
ground substance, primarily collagen, is the cement that gives our tissues form and substance. The
state (the colour) of decolourisation was supposed to be the same in both samples. The black and
white copies could be coloured in by students to aid memory of the heart structure. The colour of the
DCPIP solution becomes darker when oxidised and may result in difficulty to obtain the observation
at the end of the experiment. Share to Twitter Share to Facebook Share to Pinterest. Measuring
Vitamin C content in different substances. Our customer service team will review your report and
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right resource. Cholesterol synthesis: Enzyme 7-?-hydroxylase involved during cholesterol
biosynthesis requires Vit.C. Haemoglobin synthesis: Vitamin C help absorption of iron from intestine
which is required for hemoglobin synthesis. However, many animals can synthesize ascorbic acid
from glucose by uronic acid pathway. The student could also improve by making a null hypothesis. It
is for this reason that the foods cooked in copper utensils lose ascorbic acid quickly. Hope you enjoy
it:) ?15.00 Reviews Select overall rating (no rating) Your rating is required to reflect your happiness.
These include the synthesis of collagen, a deficiency of which leads to the most notable disease of
vitamin c loss, scurvy. With this in mind I would suggest that this experiment is in adequate to
measure vitamin c and other methods should be employed. Thus, the fruit juices should only be
prepared right before beginning the experiment to ensure the freshness of the fruit juices and that the
vitamin C content of the fruit juices are highest during the experiment. Join our team of reviewers
and help other students learn. Helpful for lesson preparation for non-specialist science teachers. There
is even more collagen in the skin, bones, teeth, blood vessels, eye, heart, and, in fact, pretty much in
all parts of the body. All posters have been provided in a choice of black and white or colour. Had I
had time I would simply have bought the orange juice the day before and kept it out of the fridge.
This water could have had an effect on our results by diluting the orange juice.
If starch is placed in the solution the iodine and triiodide molecules will react with starch to form a
blue-black complex. The pipettes used to measure DCPIP volume could have slightly different scales-
therefore one pipette should be used for DCPIP volume measure. Tomatoes, blackcurrants,
strawberries, blueberries, cranberries, pineapples, kiwi fruit, papaya, mangoes, guavas and citrus
fruits are also excellent sources of vitamin C. Both posters have been provided in an A3 and A4
format to allow for printing in either size. The worksheets encourage independent learning as the
pupils can work through them following the clear instructions given. Pasteurized apple, orange,
grape, pomegranate, tomato and carrot juices contain substantial amounts of the minerals potassium,
phosphorus and magnesium. We then took the mixture and filtered it through filter paper into a
beaker. However, when the all the vitamin C is oxidized, iodine and triiodide will be present. Write a
review Update existing review Submit review Cancel It's good to leave some feedback. The
worksheets are provided in both an A4 and A3 format. Vit-C prevent the oxidation of cellular
components by the effect of free radicals, peroxides, superoxides etc. Vitamin C in the body and an
experiment to measure Vitamin C in cabbage. The response is written in a very clear and concise way.
With this in mind I would suggest that this experiment is in adequate to measure vitamin c and other
methods should be employed. DCPIP is blue when oxidized and colourless when reduced. This is
due to the fact that least volume of orange juice is needed to decolourise and reduce the blue DCPIP
solution. This activity can be completed by students independently as all instructions are given on the
sheets. Upload Read for free FAQ and support Language (EN) Sign in Skip carousel Carousel
Previous Carousel Next What is Scribd. This is prepared using both textbooks and exam mark
scheme. Immunological function: Vitamin C has been found to be involved in the stimulation of
Antibody formation and enhancement of phagocytosis. No-pre scientific tests are done which would
increase the candidates mark to identify any confounding factors or biases and how the student
would take this into account whilst setting out the experiment. Whilst we added the cabbage mixture
to the DCPIP we saw it turn from blue to pink, this was the end point of the experiment. The
equivalence point and colour changes of indicators are not instant and it is difficult to access when
the reaction has finished. It is thought that ascorbic acid reacts with these free radicals preventing
them from causing damage to cells. The enzyme L- gluconolactone oxidase is required to convert L-
glucono-1,4-lactone into L-ascorbic acid in final step of the pathway. Therefore while the first few
vitamin C molecules don’t require a large amount of heat energy to break down, the last few, with a
small surface area and low concentration, would take a huge amount of heat energy to break down
which we did not provide. So by increasing the temperature I will expect to need less DCPIP to
change the colour of the orange juice to the standard I have chosen (when the solution has turned a
purply-brown colour). Thus my actual set of results and the method for the final experiment differ
somewhat from my preliminary results and method. Water was used as the control sample to
illustrate that only a substance containing vitamin C could decolourise DCPIP. Thus, the fruit juices
should only be prepared right before beginning the experiment to ensure the freshness of the fruit
juices and that the vitamin C content of the fruit juices are highest during the experiment.