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    Batac, Joshua Kyle C. (Bio 024) Lab Activity 11 - Lipids

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    This document discusses lipids and includes a laboratory activity testing the properties of lipids. The activity tests the solubility, translucent spot formation, acrolein smell, and Huble's test results of clarified butter, vegetable oil, and linseed oil. The conclusions drawn are that lipids are soluble in organic solvents but not water, form translucent spots, produce an irritating smell when heated, and linseed oil is more unsaturated than cottonseed oil based on Huble's test results.

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    Batac, Joshua Kyle C. (Bio 024) Lab Activity 11 - Lipids

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    joca.batac.up
    19 views4 pages
    This document discusses lipids and includes a laboratory activity testing the properties of lipids. The activity tests the solubility, translucent spot formation, acrolein smell, and Huble's test results of clarified butter, vegetable oil, and linseed oil. The conclusions drawn are that lipids are soluble in organic solvents but not water, form translucent spots, produce an irritating smell when heated, and linseed oil is more unsaturated than cottonseed oil based on Huble's test results.

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    BATAC, JOSHUA KYLE C. (BIO 024) LAB ACTIVITY 11_ LIPIDS

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    This document discusses lipids and includes a laboratory activity testing the properties of lipids. The activity tests the solubility, translucent spot formation, acrolein smell, and Huble's test results of clarified butter, vegetable oil, and linseed oil. The conclusions drawn are that lipids are soluble in organic solvents but not water, form translucent spots, produce an irritating smell when heated, and linseed oil is more unsaturated than cottonseed oil based on Huble's test results.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    19 views4 pages

    Batac, Joshua Kyle C. (Bio 024) Lab Activity 11 - Lipids

    Uploaded by

    joca.batac.up
    This document discusses lipids and includes a laboratory activity testing the properties of lipids. The activity tests the solubility, translucent spot formation, acrolein smell, and Huble's test results of clarified butter, vegetable oil, and linseed oil. The conclusions drawn are that lipids are soluble in organic solvents but not water, form translucent spots, produce an irritating smell when heated, and linseed oil is more unsaturated than cottonseed oil based on Huble's test results.

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    NAME: BATAC, JOSHUA KYLE C.

    BLOCK: 16
    DATE: BIO 024 LABORATORY

    PHINMA UNIVERSITY OF PANGASINAN


    COLLEGE OF ALLIED HEALTH SCIENCES
    DEPARTMENT OF NURSING
    ACTIVITY NO. 11 - LIPIDS

    I. DISCUSSION:
    Lipids (Importance and Characteristic of Lipids & Classification) In of Lipids
    Disorders Associated with Lipids and Lipoproteins
    Fatty Acids (Classification, Physical Properties, & Chemical Reactions)
    Triacylglycerol (Classification and Nomenclature & Physical and ChemicalProperties)
    Phospholipids
    Cholesterol
    Qualitative Tests for Lipids

    II. QUESTION (2points each):

    1. What are the general characteristics of Lipids?


    Are a remarkably diverse class of fat-soluble, water-insoluble (hydrophobic) molecules that
    function as hormone precursors, hormone sources, and energy-storing agents. Triglycerides
    (neutral fats), phospholipids, steroids, and eicosanoids are the four primary categories of
    lipids. They are the only class of biological macromolecules that are not polymers because
    they are not produced from repeating monomers.

    2. What is the importance of Lipids?


    Because they supply energy, essential fatty acids, and fat-soluble vitamins—which are often
    linked to fats—they are vital for a diet. They also improve the taste, palatability, and satiety of
    the diet.

    3. Give examples of disorders associated with Lipids and Lipoproteins.


    Disorders pertaining to the metabolism of lipids and lipoproteins are frequently linked to
    obesity, the metabolic syndrome, and diabetes.
    4. Identify the properties of Fatty acids and Triacylglycerol.
    They are soluble in organic solvents but insoluble in water, hydrophobic, and non-polar.
    Compared to water, it has a lower specific gravity. This is why fats and oils float on water.
    Saturated fatty acids have higher melting points than unsaturated fatty acids of the same
    length.

    5. What are the functions of Phospholipids and Cholesterol?


    By stopping the buildup of fat in the liver, phospholipids are beneficial. It is essential to the
    movement and elimination of cholesterol from cells. Through the interaction of proteins, it
    creates the structural elements of the cell membrane.

    III. QUALITATIVE TESTS FOR LIPIDS

    A. SOLUBILITY TEST

    RESULT (1 point each cell):


    SOLVENT TUBE 1 (Clarified butter) TUBE 2 (Vegetable oil) TUBE 3 (Linseed oil)

    Water The clarified butter The vegetable oil The linseed oil didn’t
    didn’t mix with water didn’t mix with water mix with water and it
    and it went to the top. and it went to the top went to the top.

    Ethanol The mixture turned clear Vegetable oil turned It turned clear when
    after the clarified butter clear after being mixed ethanol was added to
    was combined with with ethanol and linseed oil and heated
    ethanol and heated in a heated in a Bunsen in a bunsen burner.
    Bunsen burner. burner.

    Chloroform After mixing chloroform Chloroform was added Chloroform was


    to clarified butter, the to vegetable oil, and added to linseed oil,
    solution became clear. the mixture turned and the mixture
    clear. turned clear.
    CONCLUSION (2 points): As a result, it is evident from testing the solubility of lipids that
    they are soluble in organic solvents such as ethanol or alcohol as well as chloroform but
    insoluble in water.

    B. TRANSLUCENT SPOT TEST

    RESULT (1 point each cell):


    SAMPLE RESULT

    Clarified butter Formation of transluclent spot

    Vegetable oil Formation of translucent spot

    Linseed oil Formation of translucent spot

    CONCLUSION (2 points): Both its boiling point and refractive index are high.

    C. ACROLEIN TEST

    RESULT (1 point each cell):


    SAMPLE RESULT

    Clarified butter Pungent, irritating smell

    Vegetable oil Pungent, irritating smell

    Linseed oil Pungent, irritating smell

    CONCLUSION (2 points): The smell is from burning glycerol.

    D. HUBLE’S TEST
    RESULT (1 point each cell):
    SAMPLE RESULT

    Cotton seed oil Color violet precipitate does not fade

    Linseed oil Color violet precipitate does not fade

    CONCLUSION (2 points): Compared to linseed oil, less unsaturated

    REFERENCES:

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