CONFOCAL LASER SCANNING MICROSCOPE

LEICA Germany, Model : STELLARIS 5

Spesifikasi:
STELLARIS 5 is a true confocal point scanning system, including a White Light Laser as an
excitation light source, an Acousto Optical Beam Splitter (AOBS), and a highly sensitive, prism-
based spectral detection design with computer-controlled adjustable bandwidth for all
fluorescence channels.
Including TauSense, a set of tools based on fluorescence lifetime information, it provides an
additional contrast, improved image quality, and separation of spectrally overlapping
fluorophores.
LIGHTNING allows for optimal extraction of image details and maximum resolution, thus
expanding the imaging portfolio both in the classical range and beyond the diffraction limit.
Features a tandem scanning system to provide the highest axial resolution with galvanometric
scanner, switchable between resonant and non-resonant mode.

A. STELLARIS Scanhead
Equipped with Power HyD S detector, new standard for detection. All-round detector, high
performance throughout the spectrum, two times increased photon detection efficiency in the
blue-green range compared to standard multialkaline photomultiplier. Leica Si-based
technology is compatible with TauSense in combination with the white light laser.
STELLARIS Scanhead consist with:
▪ STELLARIS 5
▪ 8kHz Tandem Scanner STELLARIS 5
▪ Fluorifier Disc Basis inkl. Analysator
▪ Notch filter set VIS base
▪ Scan optics HIVISR with rotation DMi8
The scan optics module contains the complete scan optics for STELLARIS 5 on DMi8 stands
with the following features:
o Optical coating HIVISR with high transmission of > 99% throughout the visibile spectrum
ranging from 400-850 nm
o Low reflection at optical surfaces throughout the visible spectrum
o Independent optical rotation of the scanfield up to around 200° by a highly transparent
Abbé-König rotator design
o Prepared for either an FOV or a tandem scanner with Leica's parallax- free three-mirror
design
o Prepared for excitation with 405 nm cw or pulsed laser, all cw lasers emitting visible light
and white light laser
▪ Detector types: Power HyD
Sensor type:Multi-pixel silicon photo-multiplier array Detection modes: Analog, reflection,
counting, fast TauSense capability: Yes
Spectral sensitivity range: 410–850 nm
Typical photon detection efficiency (PDE): 58% @ 500 nm
▪ Power HyD S 2ch SP Core Unit
Specifications:
 o Up to five fully tunable spectral detection channels
o Maximal fluorescence detection because of unique prism design
o Detection range 410-850 nm. Spectral tuning resolution 1 nm. Minimal detection range 5
nm
o Equal brightness between channels due to very efficient W-shaped slit design
o Permits excitation scans as well as excitation-emission ("lambda-square") scans for
confocal spectroscopy applications
o Typical photon detection efficiency > 58% @ 500nm.
o Multi-pixel silicon photo-multiplier array.
o Extended dynamic range for maximal contrast and flexibility
o Analog, reflection and photon counting detection modes.
o Power counting: 0,1,2 (double) photons can be identified
o TauSense capabilities (requires a pulsed laser source): TauGating (up to 2 tunable
timegates, simultaneously). TauContrast (mean photon arrival time). TauSeparation
(lifetime-based component separation).
▪ Power HyD S SP 3rd ch
▪ Power HyD S SP 4th ch
▪ SP Light Trap
▪ STELLARIS 5 DMi8 adapter
▪ Control panel with LCDs
Programmable control panel with 6 tuning knobs and one LCD each for efficient real-time
control of all relevant system parameters. The control panel can be configured graphically by
LAS AF software for each user. Each IPS parameter can be freely assigned and the
transmission speed can be set by the user in up to three steps. There are two push buttons,
one for changing the active window in the viewer of LAS AF as well as one for turning on or off
a live scan.
▪ NIR Extension STELLARIS

B. VIS and UV Laser Diodes

White Light Laser: The extended detection range up to 850 nm plus the excitation range in the
visible from 485 nm up to 685 nm allows the application and separation of an extended range of
spectrally overlapping fluorophores, up to five simultaneously.
VIS and UV Laser consist of:
▪ Laser 405 DMOD
▪ Laser Port 405

C. Microscope Stand
Inverted research microscope with touch screen based on the DMi8 series, with motorized 6x
nosepiece, closed loop, cooling system, and spill protection. Quick switching from coarse to fine
drive. Prepared for confocal imaging with laser safety equipment.
The microscope stand consists of:
▪ DMi8 CS Premium
o Inverted research microscope based on the DMi8 series, with motorized nosepiece and
closed loop. Prepared for confocal imaging.
 o Motorized 6-fold fluorescence turret with automated detection equipped with Leica
Radio Frequency Identification Device and motorized shutter
o Suitable for lenses with magnifications from 1.25x to 100x
o Microscope controller CTR Box Advanced
o SmartMove for easy control in xy and z
o Fluorescence Intensity Manager (FIM)
o All microscope parameters are displayed on the LCD
o Quick switching from coarse to fine drive
o Motorized 6x nosepiece
o Camera port (prepared) on the right side
o Additional Infinity Port on the right side
o Nosepiece with cooling system
o Spill protection for motorized nosepieces on DMi8 with sealing ring and collecting
container
o Condenser S1/S28
o Condenser head S28/0.55
o Optical equipment for with brightfield light source, as well as interference contrast (ICT)
prepared for confocal microscopy:
It consists in detail of:
o LED brightfield illumination with TTL shutter
o Motorized prism wheel for ICT
o Filter System Analyzer for ICT
o Motorized polarizer
o Eyepieces HC PLAN 10/25 BR M
o Connection for lamp house, straight for DMI
o Immersion oil F
o Accessories and holder for confocal scanner
o Laser safety equipment

▪ Transmission axis
▪ Bino Tube
▪ Closed Loop Focus with AFC
▪ BF detector for DMI
Transmitted light detector (TLD) for TCS SP8 DMI 6000 and DMI 6000 CEL Detector for
acquisition of transmitted light images with interference contrast, phase contrast (DMI 6000
only) or bright field illumination (with laser scanning)
Contains:
o Photomultiplier tube
o Motorized shutter
o Software controlled switching to TLD detection
▪ Leica LED 3
▪ Scanning stage inv. Universal
▪ Cable Scanning Stage, 3 m, 90°
▪ XY advanced board for scanning stage
▪ Univ.hold.frame K:0311.200
▪ Optical Table 900 x 900 actively damped
▪ Base Plate DMi8
▪ Filter Cube RHOD
Filter cube RHOD bandpass Excitation: BP 546/10 Emission: BP 585/40
▪ Filter Cube GFP
Filter cube GFP bandpass Size: for DMi8 Excitation: BP 470/40 Emission: BP 525/50
▪ Filter cube LED 405
Filter Cube LED 405, size P
Excitation: 405/60
Dichroic: 455
Emission: 470/40
▪ Oko UNO Stage Inc Set CO2 Inv K-Frame
▪ Air Compressor IR opt. table act. 220V

D. Objective Lens and Accesoris

Consist of:
▪ Obj. HC PL FL 1.25x/0.04
Objective HC PL FLUOTAR 1.25x/0.04
Free working distance: 3.7 mm
For use with and without Cover Glass
▪ Obj. HC PL APO 10x/0.40 CS2
Plan apochromatic objective with improved color correction, optimized for confocal
scanning applications. This objective is ideal for simultaneous use of 405 nm and visible
excitation up to deep red excitation. For use with 0.17 mm coverglass. Air immersion. Free
working distance: 2.56 mm.
▪ Obj. HC PL APO 20x/0.75 CS2
Plan apochromatic objective with superior color correction, optimized for confocal scanning
applications.
For use with 0.17 mm coverglass, no immersion.
Free working distance: 0.62 mm.
▪ HC PL APO 40x/1.25 GLYC CORR CS2
Plan apochromatic glycerol immersion objective with superior color correction, optimized for
confocal scanning. Free working distance: 0.35 mm. Correction collar for use with cover
glass (0.14-0.19 mm), glycerol immersion and within the temperature range 23-37°C.
▪ Obj. HC PL APO 63x/1.40 OIL CS2
Fits SP8, STELLARIS 5, STELLARIS STED and STELLARIS 8.
Plan apochromat oil immersion objective with superior color correction, optimized for
confocal scanning applications.
Free working distance: 0.14 mm.
▪ Type G Immersion liquid, 10 mL
▪ Type F Immersion liquid, ISO 8036
▪ IC Condenser Prism K10
▪ IC Condenser Prism K3
▪ IC Prism E
▪ IC Prism A
▪ IC Prism C
▪ IC Condenser Prism K7

E. Software and Workstation

Consist of:
▪ High brilliance monitor
o Display size (diagonal): 95 cm (37,5 inch)
o Resolution (native): 4K-Wide 3840 x 1600 @ 60 Hz
o Contrast ratio: 1000:1 static, 5000000:1 dynamic
o Display: IPS with LED-Backlight
o Angle: 178° horizontal, 178° vertical
o Proportion: 21:9
o Display range (metric): 87,97 x 36,65 cm
o Brightness: 300 cd/m²
o Type of display input: 1 DisplayPort, 1 HDMI, 1 USB Type-C
o Point spacing: 0,229 mm
o Reaction time: 5 ms grey to grey
▪ Workstation Premium
Mid range workstation HP Z4G4 with CUDA support for average workloads. Can be combined
with DIVE but not with any other advanced imaging modality (DLS, FALCON, STED). Good
performance of Navigator and Lightning, very good performance of Dynamic Signal
Enhancement:
Intel Xeon W-2133 processor (6 cores / 12 threads).64 GB working memory.Nvidia Quadro
RTX 5000 graphics card with 16 GB graphic card memory and 3072 CUDA cores.fast 256 GB
SATA SSD system drive (Windows, LAS X), very fast 1 TB M.2 SSD hard drive for temporary
storage of data (transient data container of LAS X, not for the user) and normal 6 TB HDD drive
for storage of data.Intel I350-T2 network adapter. HP 9.5mm Slim SuperMulti DVD writer.
Windows 10 IoT Enterprise LTSC 2019. Keyboard, mouse und mouse pad.
The specifications of the PC may change due to technical innovations.
▪ Computer table small incl. Rack
Desktop made of multiplex wood with décor.
Solid steel frame made of square tube with finish.
Width 900mm, Depth (including cable duct) 1.000mm, Hight 770mm
Includes storage rack for microscope electronics and accessories.
Core Functionality
Image Compass:
▪ Simplistic user interface, even for complex experiments
▪ Set up of multicolor experiment with one click per fluorophore
▪ Maximal signal yield through optimal acquisition settings selected automatically
▪ Multidimensional acquisition, full control of motorized hardware
TauSense:
▪ Instant access to average arrival time (AAT) and intensity information in every experiment
(TauContrast)
▪ Differentiate between desired and unwanted fluorescence signal (TauGating)
▪ Separate fluorophores using lifetime based information (TauSeparation, TauScan) Dynamic
Signal Enhancement
▪ Uncompromized temporal resolution up to 290 fps maintaining optimal signal to noise ratio
Lightning:
▪ Lightning basic detection package. Fully automated online data acquisition with super-
resolution down to 120 nm. Adaptive image quality determination and reconstruction
Navigator:
▪ Navigator basic package. Spiral scan to easily generate an overview of your specimen.
Stitching plus mark and find functionalities
Lambda scan:
▪ Acquisition of emission spectrum using spectral detectors. Acquisition of excitation
spectrum using white light laser
Lambda-lambda scan:
▪ Acquisition of full excitation-emission spectrum
Z intensity compensation:
▪ Laser power and/or detector gain adjustments within z stacks
▪ LAS X STELLARIS Control Software
▪ LAS X 3D Visualisation: Fast, GPU-based processing of large 3D stacks, unique clipping tool
▪ LAS X Lightning Expert: Customizable image information extraction by adaptive
deconvolution
▪ LAS X Dye Finder
▪ LAS X Assay Editor: Pre-configured specimen carriers
▪ LAS X 2D Analysis: 2D/3D multi channel analysis and classification
▪ LAS X AiviaMotion
▪ Aivia Go

Application: Live Cell Imaging, 3D Imaging, FRET, FRAP, confocal imaging, fluorescence,
multicolor, Light Sheet,

Sudah Termasuk:
UPS ICA SE 6100
Capacity: 6000 VA / 5400 W
AC Voltage: 120 ~ 276 V; 1 Phase
AC Frequency: 45 ~ 65 Hz
AC Protection: 40 A
DC Voltage: 192 V
DC INT Battery: 16 x 12 V, 7 Ah
DC Protection: FUSE
Opera Temp: 0 – 40 °C
Dimension(DxWxH): 502 x 250 x 616 mm
Weight: 67 Kg

"Warranty : 1 tahun"
"Garansi : Preventive Maintenance selama seumur hidup alat"