Method 6.0 2014
Method 6.0 2014
Method 6.0 2014
UREA
2: Principle
The sample is suspended in water with a clarifying agent and filtered. The urea content
of the filtrate is determined after the addition of 4-dimethylaminobenzaldehyde (4-
DMAB) by measuring the optical density at a wavelength of 420nm.
3: Reagents
3:1 4-DMAB solution: dissolve 1.6g of 4-DMAB in 100ml of 96% ethanol and add 10ml of
hydrochloric acid (d=1.19g/ml).
3:2 Carrez solution I: dissolve in water 21.9g of zinc acetate dihydrate, Zn(CH3COO)2
2H2O and 3g of glacial acetic acid. Dilute to 100ml with water.
3:3 Carrez solution II: dissolve in water 10.6g potassium ferrocyanide, K4 Fe (CN)6 3H2O.
Dilute to 100ml with water.
3:4 Active carbon which does not absorb urea (check before use).
3:5 Urea, 0.1% solution (w/v).
4: Apparatus
4:1 Mixer (tumbler): approximately 35 to 40 rpm.
4:2 Test tubes: 160 x 16 mm with ground-glass stoppers.
4:3 Spectrophotometer.
5: Procedure
5:1 Dissolution of sample
Weigh out 2g of the prepared sample to the nearest mg with 1g of active carbon (3:4) and
transfer to a 500ml graduated flask. Add 400ml of water and add 5ml Carrez solution I
(3:2), followed by 5ml Carrez solution II (3:3), mixing well between additions. Dilute to
volume with water, mix well and filter.
5:2 Determination
Transfer 5ml of the transparent colourless filtrate to a ground-glass stoppered test tube,
add 5ml of 4- DMAB solution (3:1) and mix. Place the tubes in a water bath at 20 C (±
4 C) and allow to stand for 15 minutes. Measure the optical density of the sample
solution with the spectrophotometer at 420nm. Compare with the blank test solution of
the reagents.
6:0/1
6: Expression of Results
6:1 Determine the amount of urea in the sample using the calibration curve.
7: Repeatability
The difference between the results of 2 parallel determinations must not exceed 10%
relative to the higher value.
8: Observations
8:1 In the case of contents of urea exceeding 3%, reduce the sample to 1g or dilute the
original solution so that there are not more than 50mg of urea in 500ml.
8:2 In the case of low contents of urea, increase the sample as long as the filtrate remains
transparent and colourless.
8:3 If the sample contains simple nitrogenous compounds such as amino acids, the optical
density shall be measured at 435nm.
NOTE: If the sample is highly coloured the proportion of activated charcoal must be
increased up to 5g. The final solution after filtering should be colourless.
6:0/2
Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.
Alternative Proxies: