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    BD FACSymphony A3 Tech Specs

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    endale gebregzabher

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    © All Rights Reserved
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    BD FACSymphony A3 Tech Specs

    Uploaded by

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    60 views4 pages

    BD FACSymphony A3 Tech Specs

    Uploaded by

    endale gebregzabher

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    BD FACSymphony A3 Cell Analyzer ™

    Special Order Research Product


    The BD FACSymphony™ A3 Cell Analyzer is a novel high- wavelengths and power ratings, up to ten photomultiplier tubes
    parameter system that leverages the inherent benefits of flow (PMTs) per laser arranged in decagon arrays and with numerous
    cytometry and enables the simultaneous measurement of up filter combinations. BD FACSymphony™ Systems are also field
    to 30 different characteristics of a single cell. The cell analyzer upgradeable to support the addition of any new advances in
    is manufactured as a Special Order Research Product and is technology.
    highly customizable with up to five different lasers of chosen
    Technical specifications
    Optics Performance
    Excitation optics Fluorescence sensitivity
    Optics layout accommodates up to five lasers simultaneously May vary dependent on the combination of laser and
    filter selections
    Select from 26 different lasers of varying power levels, ranging
    from 20 mW to 1,000 mW Fluorescence resolution
    Most common laser wavelengths available are 355 nm, 405 nm, Coefficient of variation (CV)
    488-nm, 561 nm and 637 nm PI: Area, <3.0%, full G0 /G1 peak for PI-stained chicken
    erythrocyte nuclei (CEN)
    Flow cell
    Rectangular quartz cuvette: Internal cross-section, 430 x 180 μm Fluorescence linearity
    External quartz cuvette surfaces are anti-reflective coated for Doublet/singlet ratio: PI-stained CEN: 1.95–2.05 (488-nm laser)
    optimal transmission of laser light. Fixed optical assembly with
    spatially separated laser beams. Forward and side scatter sensitivity
    Sensitivity enables separation of fixed platelets from noise
    Emission optics
    Forward and side scatter resolution
    Optical coupling
    Scatter performance is optimized for resolving lymphocytes,
    Emitted light from the gel-coupled cuvette is delivered by fiber
    monocytes and granulocytes
    optics to the detector arrays. The optical pathways use signal
    reflection to maximize signal detection. Each detector array is
    Side scatter resolution
    equipped with appropriately matched optical filters for light
    Scatter performance enables separation of 0.5-μm beads
    collection.
    from noise

    Forward scatter detector


    Acquisition rate
    Photodiode with a 488/10 bandpass (BP) filter for the 488-nm
    40,000 events/second with beads
    laser

    Side scatter detector Data management


    PMT with a 488/10 BP filter for the 488-nm laser
    Software
    Fluorescence detectors BD FACSDiva™ Software v9.1 or later
    Flexible detector array technology enables user-defined
    detection configurations. Filters and mirrors are interchangeable. Workstation†
    HP® Z2 G4 Mini Workstation
    • Intel® Core™ i7-8700 processor
    Fluidics
    • 1-TB NVMe SSD
    Sample flow rates • 16-GB RAM
    Front-button panel provides three modes: RUN, STANDBY and • Microsoft® Windows® 10 Enterprise
    PRIME. • 2019 LTSC (64-bit) OS
    Continuously adjustable flow rate, plus three preset flow rates:
    LO: 12 μL/min
    MED: 35 μL/min
    HI: 60 μL/min

    Fluidic reservoirs
    Autoclavable 10-L sheath and waste containers are provided

    Minimum configuration listed. Workstation may include upgraded specifications.


    †
    Options Installation requirements
    BD® High-Throughput Sampler (HTS) Option Instrument dimensions (W x D x H)
    Increase lab productivity by utilizing this option to acquire 83.8 x 76.2 x 80 cm (33 x 30 x 31.5 in)
    samples from a 96- or 384-well microtiter plate.
    Weight
    Acquisition throughput
    167.9 kg (379 lb)
    High-throughput mode: Less than 15 minutes per
    microtiter plate using 2 second acquisition Power
    Standard mode: Less than 44 minutes using 10 Operation at 100/115/230 VAC and 50 or 60 Hz
    second acquisition
    Temperature operating range
    Carryover
    Between 19 and 26°C (66 and 79°F)
    High-throughput mode: <0.5%
    Standard mode: <0.75% Operating humidity
    10%–90% relative humidity (noncondensing)
    BD FACSFlow™ Supply System
    Automated fluidics system that includes a rolling cart and two Heat dissipation
    20-L Cubitainer® packages 2,701 BTU/hour

    Electrical requirements
    BD requires one dedicated circuit for the cytometer and the
    computer system (including printer), with a dedicated AC source
    not shared with any other equipment. The instrument will be
    powered from the line conditioner supplied by BD Biosciences.
    Class 1 Laser Product.
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

    23-22554-00

    BD Life Sciences, San Jose, CA, 95131, USA

    bdbiosciences.com
    BD, the BD Logo, FACSDiva, FACSFlow and FACSymphony are trademarks of Becton, Dickinson and Company or its affiliates.
    All other trademarks are the property of their respective owners. © 2020 BD. All rights reserved.

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