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    Expt. 04-Ph. Cology-II-SRD

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    adtyaqmsec
    SRD sir Odhisha Assistant professor

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    Expt. 04-Ph. Cology-II-SRD

    Uploaded by

    adtyaqmsec
    3 views2 pages
    SRD sir Odhisha Assistant professor

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    SRD sir Odhisha Assistant professor

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    3 views2 pages

    Expt. 04-Ph. Cology-II-SRD

    Uploaded by

    adtyaqmsec
    SRD sir Odhisha Assistant professor

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    Experiment No: 04 Date:

    STUDY OF DIURETIC ACTIVITY OF DRUGS USING RATS/MICE

    Aim of the experiment: To screen the study drug for diuretic property in albino rat.

    Animal used: Albino rat

    Drug used: Frusemide (5mg/g volume)

    Equipment use: Metallic cage, Measuring cylinder.

    Method:

    Diuretics property of given drug can be demonstrated both in-vivo and in vitro experiment.
    The in vitro method includes isolated perfuse kidney of Dog, Guineapig, Rabbit &in vivo
    method are performed in Dog, Rat, Mice &Rabbit. Both anaesthetized and unanaesthetised
    group are preferred as they not interfere by anaesthetics. Recently punctured analysis and
    micro perfusion technique has been developed for screening of drug for diuretic properties.

    Procedure:

    The healthy albino rat of either sex was weighed (about 150-200gm) and divided into 2
    groups. Each group consisting of 4 albino rats. One group was treated as control and other
    one is test. All the albino rats were deprived for water and food. However, they are
    administered normal saline 25ml per kg body weight orally with the help of feeding tube.
    After few minutes the test drug frusemide was administered intraperitonially at the dose of
    5 mg kg body weight, to the test group of animals and drugs administration time was noted.
    But in control group animals no drug is administered. The animals were placed in metallic
    cage. The volume of urine in each group was calculated at an interval of 30 minutes to the
    period of 2 hours.

    Page | 13
    Experiment No: 04 Date:

    Urine volume Urine volume Urine volume Urine volume Total Urine
    B.W.
    GROUP S. No. after 30 min after 60min after 90min after120min volume
    (gm)
    (ml) (ml) (ml) (ml) (ml)

    1 190 1.0 0.9 0.6 0.6 3.1

    2 180 0.8 0.2 0.7 0.8 2.5

    3 175 0.7 0.6 0.2 0.7 2.2


    A
    4 180 0.9 0.7 0.6 0.1 2.3

    5 170 0.8 0.6 0.2 0.4 2

    6 185 0.7 0.4 0.2 0.6 1.9

    1 180 2.9 2.5 2.3 2.0 9.7

    2 168 2.3 2.4 2.1 1.9 8.7

    3 190 2.8 2.6 2.3 2.1 9.8


    B
    4 185 2.7 2.5 2.4 1.8 9.4

    5 175 2.6 2.6 2.2 2.0 9.4

    6 170 2.7 2.2 2.4 2.1 9.4

    Report: From the above observation it was observed that frusemide enhances the diuretic
    properties. Hence the supplied drug frusemide produces diuretic effect in albino rat as compare
    to the control group.

    Mr. Smrutiranjan Dash


    Assistant Professor
    Department of Pharmacology

    Page | 14

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