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Subject Syllabus
Laboratory Best Practices
Course: B.Sc. Semester 1
Prerequisite: Basic knowledge of Biology, Physics and Chemistry
Rationale: - Curriculum will provide a general understanding of the required laboratory ethics and practices. Teaching and Examination Scheme
Teaching Scheme Examination Scheme Total
Lect Tuto Lab Hrs/ Credit Internal Marks External
ure rial Hrs/ Marks Hrs/ Hrs/ Wee Week Week k T CE P T P
1 - 2 - 2 20 30 50
Course W - Weightage (%), T - Teaching W T
Content 1 Unit 1 Introduction to Good Laboratory Practices (GLP) 12 12 and Standard Operating Procedures (SOP) History, Scope, and Fundamental Points of GLP (Rules and results, Quality assurance, Quality Control, Quality engineering),Laboratory hierarchy and SOP Levels of Laboratories, Log Book Maintenance, Basic SOPs for instrument handling and Maintenance, Personal Protective Equipment (PPE), record technical and administrative improvements. 2 Unit 2 Laboratory rules and Protocols 13 12 General Rules/Protocols for Lab Safety measures (laboratory safety showers, eyewash stations, and fire extinguishers, exit routes), Precaution and Safety in handling of chemicals (chemical inventory list, applicable safety data sheets (SDS), departmental laboratory safety manual), Laboratory tools (records of certification, maintenance, or repairs), Glassware and instruments. 3 Unit 3 Record Keeping and Interpretation 12 12 Keeping data records, its analysis by using statistical and mathematical tools. Result analysis and its interpretation (Raw data and Data Collection, Final report, summary of findings, discussion and reference. 4 Practical 13 12 Basic knowledge of analytical chemistry (Molarity, Normality, Solution preparation, sample collection, labelling, Use of Weighing balance and its calibration and maintenance, Storage and Transport Chemicals Safely, applicable safety data sheets, Personal Protective Equipment (PPE).Sample/Chemical/e-waste disposal
Recommended Textbooks and References:
1. Handbook Good Laboratory Practices-World health organization (WHO) 2. Life science protocol manual (2018)-DBT star college scheme 3. Guidelines for good laboratory practices-Indian council of medical research, New Delhi (2021) Learning Objectives:
• To teach the students safety handling and regulation of laboratory facility.
• To learn how to record, keep and analyze laboratory data with accuracy. • To practice minimization of Errors related with handling of laboratory accessories and equipment’s. • To learn Standard Operating Procedures (SOPs) Laboratory equipment’s.
Course outcomes:
• Understand the basic calibration and handling of instrumentation in
laboratory. • Safely practice, basic laboratory procedures and protocols while on job laboratory setting. • Maintain laboratory records, compliance with current industry standards and maintain audit record