Eppendorf Mastercycler

Service Manual · English
Valid for devices with the application software up to version 1.5

Inhalt / Contents / Sommaire / Indice / Indice
Service Manual Mastercycler® ep realplex 6302 · English · 6302 900.521-07

Safety 1
PACT information sheets 2
Error lists / Troubleshooting 3
Disassembly / Assembly 4
(blank) Alignment / Adjustment 5
Diagrams 6
Spare parts list 7
Technical data 8
Care and maintenance 9
(blank) Descriptions 10
Service Manual Mastercycler® ep realplex 6302 · English · 6302 900.521-07
eppendorf Service Manual
Mastercycler® ep 6302 – Contents
Contents
1 Safety
Safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Shipping of devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Decontamination (Certificate) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
2 PACT-Information sheets
3 Error lists / Troubleshooting
User error list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

Device error list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Differentiating feature of service functions within realplex software and commissioning software. . . . . . . . . . 3-32
realplex Application Software V1.5: Call up the service functions of realplex or Mastercycler. . . . . . . . . . . . . . 3-32
Service functions of the realplex module from firmware version 3.36 onwards . . . . . . . . . . . . . . . . . . . . . . . . . . 3-33
Service functions of the Mastercycler from firmware version 2.601 onwards . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-37
Software update for the Mastercycler/realplex module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-39
Temperature Validation/Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-39
Commissioning software V1.14 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-40
RealSpector Software V 1.41 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-45
Technical differences between the realplex application software V1.0, V1.5 and V2.0. . . . . . . . . . . . . . . . . . . . . 3-47
4 Disassembly / Assembly
Disassembly of upper part of housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

Disassembly of the Frame with heating foil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Disassembly of the insulation plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Disassembly of the rubber sleeve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Cleaning of the lower lens array in the light path . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Replacement of the PCB Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Procedure prior to installation of the PCB Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Procedure after replacement of PCB Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Firmware update of PCB Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Replacement of the PCB Amplifier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Disassembly LED Matrix component. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
Change of multiplier module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
Replace light barrier(s) or motor for filter wheels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-13
Disassembly of light barrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15
KS/1209 0-1
Mastercycler® ep 6302 – Contents
Disassembly of filter rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15

Contents
Upgrading of a Mastercycler ep to a body cycler for realplex module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15

Pretesting of Mastercycler ep . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15
Upgrading of Mastercycler ep to a Mastercycler realplex system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
Disassembly of Heated lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
Disassembly of stop eccenter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
Disassembly of X-drive (if present) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
Installation of mounting set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-17
Mount realplex module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-18
Alignment of realplex module with Thermoblock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-19
Mounting of realplex on a body cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-20
6 Diagrams
General circuit diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6302_900.491-01

PCB_Control-Online . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6302_802.007-11
PCB_Amplifier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6302_804.000-04
7 Spare parts list
Spare parts list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1

Recommended spare parts - First equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4
8 Technical data
Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
9 Care and maintenance
Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
Returning devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
Specification for on-site disinfection of the Mastercycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Mastercycler modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Disinfection procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Maintenance of PCR Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-3
Operational Qualification (OQ) including electrical test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Extra Maintenance Service of PCR Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Description: Maintenance of PCR Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-5
Description: Extra Maintenance Services of PCR systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-6
Electrical safety test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-7
0-2 KS/1209
Mastercycler® ep 6302 – 1 Safety
Explanation of warning and hazard symbols in this Service Manual
Symbol Meaning
DANGER
Risk of electric shock with potential for severe injury or death as a consequence.
DANGER
Risk of explosion with potential for severe injury or death as a consequence.
DANGER
1 Safety
Risk of burn.
DANGER
Biohazard with potential for risk to health or death as a consequence.
WARNING
Warning of potential cuts.
WARNUNG
Warning of potential crushing injury.
WARNING
Warning of potential injury.
CAUTION
Refers to slight risk or risk of material damage.
Refers to particularly useful information and tips.
Symbols used
Symbol Meaning
DANGER
Risk of burn.
MSTM/2307 1-1
Danger! Electric shock.

→ Switch off the device and disconnect the power plug before opening the device, performing
work on electrical connections or starting maintenance or cleaning work.
Risk of explosion!!
→ Do not operate the device in rooms where work is being carried out with explosive substances.
→ Do not use this device to process any explosive, radioactive or highly reactive substances.
Risk when handling toxic or radioactively-marked liquids or pathogenic germs.

→ Follow national regulations governing the handling of these substances.
→ For complete instructions regarding the handling of germs or biological material of risk group II
or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization,
current edition of the Laboratory Biosafety Manual).
1 Safety
Warning! Risk of injury from sharp-edged components.

→ If the Mastercycler realplex module is propped open,sharp-edged components are accessible.
When the module is being cleaned, the sharp edges of the Rollflex cable and the guide rail may
lead to injuries.
Warning! Burns from hot thermoblock and heated lid.

→ The thermoblock and frame with heating foil very rapidly reach temperatures of 95°C respec-
tively 105 °C.
→ Risk of burns!
→ Keep realplex-Modul closed until the temperature of the thermoblock is below 30 °C.
Warning! Risk of burns.

→ The thermoblock, frame with heating foil and micro test tubes/PCR plates very rapidly reach
temperatures of above 105 °C.
→ Allow the block and the frame with heating foil to cool down to 30 °C before opening the device.
→ Symbol on thermoblock: warns that the thermoblock may be hot (triangle pointing downwards)
or that the frame with heating foil may be hot (triangle pointing upwards). This Symbol
shows that during gradient operation, the temperature on the right-hand
side is higher than on the left-hand side.
Warning! Injury from trapping or crushing.

→ Do not place your fingers between the lid and the housing when opening and closing the device
or when opening it into the cleaning position.
→ Do not reach in under the open lid.
→ Ensure that the prop is correctly engaged so that the device remains in its open position.
Caution! Damage to electronic components and the lens from condensation.

After moving the Mastercycler ep realplex from a cool environment to a warmer one, condensation
may form in the device.
→ Allow the Mastercycler ep realplex to warm up for at least 12 hours after being moved before you
connect it to the mains power supply.
Caution! Material damage from incorrect connections.

→ Only electrical connections to devices described in the operating manual may be made.
→ Other connections are permitted only following consultation and agreement with Eppendorf AG!
Warning! Damage due to incorrect power supply.

→ Only connect the device to power sources that match the electrical specifications on the device
ID plate.
→ Use only sockets with protective earth.
1-2 MSTM/2307
Caution! Damage due to overheating.

→ Do not place the device close to sources of heat (e.g. radiator, drying cabinet).
→ Do not expose the device to direct sunlight.
→ Ensure free circulation of air by maintaining a distance of at least 10 cm on all sides of the device
from adjacent devices or walls and keep the underside of the device clear.
Caution! Damage to the device from incorrect operation.

→ Do not put any objects down on the realplex-Modul. Do not allow any liquid to get into the
device.
Caution! Damage to the thermoblock from incorrect operation.

→ Do not pour sample material straight into the thermoblock.
Caution! Damage to the thermoblock from the use of unsuitable sample tubes.
1 Safety
Unsuitable tubes may be so severely damaged that sample material is released. Particular attention
should be paid to this point when working with infectious material.
→ Use only sample tubes recommended by Eppendorf.
Warning! Risk to health if used incorrectly.

If incubation is performed incorrectly, sample material may be released and the thermoblock and the
realplex-Modul damaged.
→ Ensure that the PCR plates are correctly inserted in the thermoblock.
→ Always heat the thermoblock and the frame with heating foil simultaneously. If incubation is per-
formed with the frame with heating foil cold, micro test tubes may burst.
Warning! Poor safety due to incorrect accessories.

The use of accessories and spare parts other than those recommended by Eppendorf may impair
the safety, function and precision of the device. Eppendorf accepts no warranty or liability for
damage caused by third-party parts or incorrect use.
→ Use only original accessories recommended by Eppendorf.
Caution! Damage to the display from mechanical pressure.

→ Do not exert any mechanical pressure on the display.
Caution! Ensure that service manuals are up to date.

→ Before you start working with the device make sure that your service manual is up to date. To do
so, check the version number at www.eppendorf-support.com.
The safety regulations applicable in the lab should always be observed when installing and using the
device.
Due to its weight of 24 kg, the Mastercycler ep realplex must be positioned completely on a stable
work surface. A secure stand is required when opening and closing the Mastercycler ep realplex.
Danger! Electric shock as a result of penetration of liquid.

→ Switch off the device and disconnect it from the power supply before starting cleaning or disin-
fecting.
→ Do not allow any liquids to penetrate the inside of the housing.
→ Do not disinfect by means of spraying.
→ Only reconnect the device to the power supply once it is completely dry.
MSTM/2307 1-3
Caution! Service and maintenance by authorized personnel only.

Eppendorf AG accepts no guarantee, warranty or liability for damage resulting from improper work.
→ Only carry out repair or maintenance work if you have successfully completed relevant training
by Eppendorf AG or have the skills in repair and maintenance specific to the device.
1 Safety
1-4 MSTM/2307
Mastercycler® ep 6302 – 2 PACT information sheets
2 PACT information sheets
MSTM/2205 2-1
PACT-System
Product News Application News Commercial Info Technical Info
Mastercycler ep realplex System
Für das Mastercycler ep realplex System mit der Applikationssoftware 2.0 ist ein komplett
überarbeitetes Service Manual auf der Internetseite www.eppendorf-support.com verfügbar. In
der Neufassung dieses Service Manuals werden erstmals das Cycler und das realplex Modul in
einem kompletten Handbuch zusammengefasst. Bitte beachten Sie, dass für den Cycler und das
realplex Modul bis Applikationssoftware 1.5 separate Handbücher zum Download auf der
Internetseite www.eppendorf-support.com verfügbar sind.
Mastercycler ep realplex system
For the Mastercycler ep realplex system with application software 2.0, a completely revised
service manual is available on our website www.eppendorf-support.com. The revised version of
this service manual combines the Cycler and the realplex module in a complete manual for the
first time. Please note that separate manuals are available for download on our website
www.eppendorf-support.com for the Cycler and the realplex module up until application software
1.5.
2008-06-02 /KS / UK Mastercycler ep realplex 8 / 01 1 (1)

Technical-Information
Contact person in HQ, Hamburg: Ingo Dietrich
Telefon: + 49 40 - 538 01 - 314
Email: dietrich.i@eppendorf.de
A new PCB Power supply unit (6321 856.509) is available for Mastercycler pro 6321 / 6325
Mastercycler ep 5341 / 5344 / 5345 and Mastercycler realplex 6300 / 6302.
The new PCB Power supply unit (6321 856.509) replaces the previous PCB Power supply 6321 856.002
and 5341 856.002.
We recommend that you include this information together with Chapter 2 of your Mastercycler Service
Manual, to have all relevant product information available in one place.
Für den Mastercycler pro 6321 / 6325, Mastercycler ep 5341 / 5344 / 5345 und den Mastercycler
realplex 6300 / 6302ist eine neue LP-Netzteil (6321 856.509) verfügbar.
Die neue LP-Netzteil ( 6321 856.509 ) ersetzt die bisherigen

LP-Netzteil 6321 856.002 und 5341 856.002 .
Wir empfehlen, diese Info Ihrem Service Manual Mastercycler Kapitel 2 beizufügen,
damit alle relevanten Produktinformationen zusammenhängend abgelegt sind.
Technical Information_6321_PCB Power supply unit (6321 856.509).doc

Technical-Information
Contact person in HQ, Hamburg: Ingo Dietrich
Telefon: + 49 40 - 538 01 - 314
Email: dietrich.i@eppendorf.de
For PCB Power supply unit (6321 856.002) a modified PCB SMPS ( 6321 895.008 ) is available.
For Mastercycler pro with PCB Power supply 6321 856.002 the PCB SMPS
(switch mode power supply) must be exchanged, if the errors D2C and/or D2D occasionally occur.
For Mastercycler ep 5341 / 5344 / 5345 and Mastercycler realplex 6300 / 6302
the previous PCB Power supply 6321 856.002 as well as the PCB Power supply 5341 856.002
are further on usable without any modifications.
We recommend that you include this information together with Chapter 2 of your Mastercycler Service
Manual, to have all relevant product information available in one place.
Für die LP-Netzteil (6321 856.002) ist eine veränderte LP-SMPS ( 6321 895.008 ) verfügbar:
Beim Mastercycler Pro mit der LP Netzteil 6321 856.002 muss die LP SMPS (Switch Mode Power Supply)
ausgetauscht werden, wenn gelegentlich die Fehler D2C und/oder D2D auftreten.
Im Mastercycler ep 5341 / 5344 / 5345 und im Mastercycler realplex 6300 / 6302

kann sowohl die bisherige LP-Netzteil (6321 856.002) als auch die LP-Netzteil ( 5341 856.002 )
weiterhin ohne Änderung eingesetzt werden.
Wir empfehlen, diese Info Ihrem Service Manual Mastercycler Kapitel 2 beizufügen,
damit alle relevanten Produktinformationen zusammenhängend abgelegt sind.
PCB SMPS 6321 895.008

on PCB Power supply
6321 856.002
Technical Information_6321_ PCB SMPS ( 6321 895.008 ). doc

Mastercycler® ep 6302 – 2 PACT information sheets

2 PACT information sheets
2-2 MSTM/2205
Mastercycler® ep 6302 – 3 Error lists / Troubleshooting
User error list
If an error occurred, a screenshot of the error message and the OdEvent.log will be needed for troubleshooting. Save
OdEvent.log before restarting the Mastercycler ep realplex system. This is the pathfinder C:/ Programs/Eppendorf/
realplex/OdEvent.log.If necessary system can be restarted now.
R.S. means reboot system.
Error code Internal error Cause Remedy

message
Error Example: These error message consist on

explanation: Text – Error 0x0257 two informations.
(Error Code = 2808) Error Code = 2808 and
Error 0x0257
Under Error Code 2808 in this list
you will find further information.
The Error 0x0257 you will find in the
device error list.
101 Internal Global database error Restart Mastercycler ep realplex

system. If error is not rectified,

restore database from backup
102 Internal Global error Notify Eppendorf Service in

Hamburg
103 Info Assay open See operating manual
104 File error Assay export Check storage medium
105 Assay import
106 File error Assay import Check storage medium
107 Internal Assay import Check storage medium
108 Initializing cycler PCR start Restart Mastercycler ep realplex

system; otherwise notify Service
110 -114 Info PCR start See operating manual
115 Info PCR start: examination See operating manual

Mastercycler program
116 PCR start: load color separation See operating manual
117 Info PCR start See operating manual
118-121 Internal PCR start Notify Eppendorf Service in

Hamburg
122 Info Assay save as See operating manual
MSTM/0408 3-1

message
123 Info Assay new See operating manual
124 Internal Assay new See operating manual
125 Internal See operating manual
126 Internal: file error Notify Eppendorf Service in

Hamburg
127 There is only one See operating manual

template
128 Internal: file error Notify Eppendorf Service in

Hamburg
130 Internal, there is only See operating manual

one template
131 Internal Application start Notify Eppendorf Service in

Hamburg
132 Error with DATABASE Application start Restart PC/LAPTOP; if nec. re-
connect install SOFTWARE
133 Logout See operating manual
134 End application See operating manual
135 PCR start See operating manual
136 - 138 PCR end See operating manual
201 File error Display/print report Reload icon; if nec. re-install

SOFTWARE
301 Internal Display/print report Notify Eppendorf Service in

Hamburg
401 Open assay dialog, folder name See operating manual
402 - 406 Open assay dialog, delete folder See operating manual
408 - 411 See operating manual
501 Cycler error Background calibration initialization Restart Mastercycler ep realplex

system; notify Eppendorf
defectiveService in Hamburg
3-2 MSTM/0408

message
502 End background calibration See operating manual
504 Start background calibration
505 Calibration background calibration Keep the realplex module closed.
506 Cycler error Incubation background calibration Restart Mastercycler ep realplex

system; notify Eppendorf Service in
Hamburg
507 Cycler error Incubation background calibration Restart Mastercycler ep realplex

Hamburg
508 Background calibration temperature Check CAN cable; restart realplex +

display Mastercycler, notify
Eppendorf Service in Hamburg

509 End background calibration
510 realplex error Start background calibration Restart Mastercycler ep realplex

Hamburg
511 Cycler error Background calibration temperature Restart Mastercycler ep realplex

display system; notify Eppendorf Service in
Hamburg
601 Cycler error Color calibration initialization Restart Mastercycler ep realplex

Hamburg
602 Start color calibration
603 Start color calibration Keep the realplex module closed.
604 Cycler error Start color calibration Restart Mastercycler ep realplex

Hamburg
605 - 605 Cycler error Color calibration incubation Restart Mastercycler ep realplex
Hamburg
607 Database error End color calibration Restart Mastercycler ep realplex

Hamburg
608 Color calibration temperature Check CAN cable, restart

display Mastercycler ep realplex system;
notify Eppendorf Service in
Hamburg
MSTM/0408 3-3

message
609 Internal End color calibration Restart Mastercycler ep realplex

Hamburg
610 Cycler error For more details refer device error

list in this section
702 Internal Notify Eppendorf Service in

Hamburg

Hamburg
802 Dye management: Delete dye

Hamburg
901 - 902 Edit/create PlateLayout NegCtrl See operating manual
1001 - 1008 Edit/create PlateLayout PosCtrl See operating manual
1101 Report dialog: Select dye See operating manual
1102 Report dialog: Select data source See operating manual
1103 - 1104 Report dialog: create template See operating manual
1105 Report dialog: delete template See operating manual
1201 - 1204 Save assay as: OK See operating manual
1205 -1206 Save assay as: Delete folder See operating manual
1207 -1208 Save assay as: Delete assay See operating manual
1209 - 1213 Save assay as: rename assay See operating manual
1301 - 2208 Edit/create PlateLayout Standard See operating manual
1401 PCR start, select background See operating manual

calibration
1501 - 1504 Edit/create PlateLayout Unknown See operating manual
1601 - 1603 User administration: Delete user See operating manual
3-4 MSTM/0408

message
1703 Background calibrations: Delete See operating manual

calibration

Hamburg

Hamburg
1801 File error Fluorescence measurement: Restart PC/LAPTOP; notify

intermediate data storage of Eppendorf Service in Hamburg
measurement data

Hamburg
2001 Fluorescence measurement Keep the realplex module closed.

2002 File error or internal Fluorescence measurement Restart PC/LAPTOP; notify
Eppendorf Service in Hamburg
2003 realplex error For more details refer device error

list in this section
2102 – 2103 Internal See operating manual

Hamburg
2201 Copy assay, OK See operating manual
2301 Create dye See operating manual
2302 - 2303 Create/change dye See operating manual
2401 Login See operating manual
2501 - 2502 Create/change sample See operating manual
2601 Database error Create sample See operating manual
2602 Delete sample See operating manual
MSTM/2307 3-5

message
2701 - 2704 Create/change user: OK See operating manual
Create/change user: OK See operating manual
2801 Abort PCR See operating manual
2802 Pause PCR See operating manual
2803 Resume PCR See operating manual
2804 CreateProcess-Errors Application start: Start file server Notify Eppendorf Service in
Hamburg
2805 CreateProcess-Errors Application start: Observer Notify Eppendorf Service in

Hamburg
2806 PCR start

2807 - 2808 Cycler error For more details refer device error
list in this section.

Hamburg

Hamburg
3201 - 3202 Application start: Restore canceled See operating manual

assay
3203 -3207 PlateLayout: Paste See operating manual
3208 File error Application start: Restore canceled Restart realplex If error is not
assay rectified, restore DATABASE from
backup
3209 - 3211 Assay new, load assay See operating manual
3301 CCommandInsertErr PCR Editor: Paste See operating manual

or
3302 - 3303 Internal PCR Editor: Paste See operating manual
3304 PCR Editor: Clear program See operating manual
3401 PCR Editor: Edit melting point
3-6 MSTM/0408

message
3501 Internal PCR Editor Notify Eppendorf Service in

(CCommandRangeEx Hamburg
ception)
3502 Internal PCR Editor See operating manual
3601 PCR Editor: Insert step
3602 Internal PCR Editor Notify Eppendorf Service in

Hamburg
3701 File error SysDlg cycler node: Save Check storage medium
3702 PrinterInit SysDlg cycler node: Print Check printer installation and
printer hardware
3703 Print SysDlg cycler node: Print Check printer installation and
printer hardware

3801 SysDlg cycler node: Validation/ See operating manual
Calib. quit
3802 SysDlg cycler node: Validation/ Read and follow OM

Calib. quit
3901 CanFlashInterface, Firmware update initialization Restart PC/LAPTOP and

DLL error Mastercycler; if nec. re-install
SOFTWARE; if nec. check
HARDWARE
3902 Firmware update: Execute
3903 CanFlash error Firmware update: Execute Restart PC/LAPTOP and

Mastercycler; if nec. re-install
SOFTWARE, if nec. check
HARDWARE
3904 FileError, CanFlash Firmware update: Check file version Restart PC/LAPTOP and
error Mastercycler; if nec. re-install
HARDWARE
4001 File error SysDlg realplex/logging: Save Check storage medium
4002 PrinterInit SysDlg realplex/logging: Print Check printer installation and

printer hardware
4003 Print SysDlg realplex/logging: Print Check printer installation and

printer hardware
4101 File error SysDlg system error/user log: Save Check storage medium
MSTM/2307 3-7

message
4102 PrinterInit SysDlg system error/user log: Print Check printer installation and
printer hardware
4103 Print SysDlg system error/user log: Print Check printer installation and
printer hardware
4201 Internal SysDlg: OK Notify Eppendorf Service in

Hamburg
4202 Internal SysDlg: Cancel Notify Eppendorf Service in

Hamburg
4203 SysDlg: Quit dialog See operating manual
4301 database error SysDlg user: Password See operating manual
4302 - 4303 SysDlg user See operating manual

4401 Database error SysDlg UserAdmin: New user Restart realplex If error is not
rectified, restore DATABASE from
backup
4402 - 4405 SysDlg UserAdmin: Change user See operating manual
4501 Cycler error Firmware update Restart PC/LAPTOP and

Mastercycler; if nec. re-install
HARDWARE
4601 Cycler error SysDlg CyclerService/Lid Restart PC/LAPTOP and

Mastercycler; check HARDWARE
configuration, if nec. check
HARDWARE
4602 Cycler error SysDlg CyclerService/Lid Restart PC/LAPTOP and

HARDWARE
4701 Cycler error SysDlg CyclerSystem/Restart Restart PC/LAPTOP and

HARDWARE
4801 PrinterInit SysDlg CyclerSystem/Valid/Calib: Check printer installation and

Print printer hardware
4802 Print SysDlg CyclerSystem/Valid/Calib: Check printer installation and

Print printer hardware
4803 File error SysDlg CyclerSystem/Valid/Calib: Check storage medium

Save
3-8 MSTM/2307

message
4901 Cycler error SysDlg: Initialization Restart PC/LAPTOP and

Mastercycler; check CAN bus cable
and terminators
5001 Realplex error SysDlg realplex Module: Restart PC/LAPTOP and

Initialization Mastercycler; check CAN bus cable
and terminators
5002 Realplex error SysDlg realplex Module Restart PC/LAPTOP and

and terminators
5101 - 5103 Internal Analysis See operating manual
5103 Internal change Analysis See operating manual
5104 File error Analysis: Save sample data Check storage medium

5201 Background/color calibration See operating manual
5301 - 5302 PlateLayout: Set filter See operating manual
5303 - 5309 PlateLayout: Add/Edit sample See operating manual
5310 - 5317 PlateLayout: Group/Ungroup See operating manual
5318 PlateLayout: Delete sample See operating manual
5319 - 5220 PlateLayout: Set subassay See operating manual
5322 Dye missing Assay import Check storage medium
5324 Internal PlateLayout: Paste See operating manual
5325 Internal PlateLayout: Paste Notify Eppendorf Service in

Hamburg
5326 Internal PlateLayout: Undo Notify Eppendorf Service in

Hamburg
5327 PlateLayout: Reference dye See operating manual
5328 PlateLayout: Edit mode See operating manual
5329 PlateLayout: Edit mode See operating manual
5330 PlateLayout: Clear all See operating manual
MSTM/0408 3-9

message
5401 - 5408 Rel/Abs quantification: Manual See operating manual

baseline
5501 MastercyclerError SysDlg Cycler/Block Restart PC/LAPTOP and

and terminators
5601 CyclerError SysDlg Cycler/realplex module Restart PC/LAPTOP and

and terminators
5701 CyclerError SysDlg CyclerService/Settings Restart PC/LAPTOP and

HARDWARE
5801 CyclerError SysDlg CyclerSystem/Sensors Restart PC/LAPTOP and

HARDWARE
5901 CyclerError SysDlg realplex Module Restart PC/LAPTOP and

and terminators
6001 Validation/Calibration Validation/Calibration start
3-10 MSTM/0408
Device error list
If an error occurred, a screenshot of the error message and the OdEvent.log will be needed for troubleshooting. Save
OdEvent.log before restarting the Mastercycler ep realplex system. This is the pathfinder C:/ Programs/Eppendorf/
realplex/OdEvent.log.If necessary system can be restarted now.
Error code Error message Cause Solution

If this does not solve the
problem, try or do the
following.
0x0050 (WIN_CAN_TRANSFER_ERROR) Communication lost Check the connection, and

Timeout occurs during a try again. If this does not
buffer transfer. help, then R. S.
(no ACK, or 8-bit data
package response)

0x0051 (WIN_CAN_CONNECTION_ERROR) Error occurs while trying Check the connection. Make
to connect. sure that target system is
(no CTS response). online and running.
0x0052 (WIN_CAN_AWAIT_CONNECT_ERR Timeout occurs while Check the connection. Make

OR) waiting for a connection. sure that target system is
(no RTS received) online and running.
0x0053 (WIN_CAN_NO_NET) No CAN net found. Make sure that the CAN
hardware is properly
installed.
0x0054 (WIN_CAN_NO_HARDWARE) No CAN hardware found. Make sure that the CAN
installed.
0x0055 (WIN_CAN_REGISTER_CLIENT) Error initialising Client. Make sure that the CAN
The maximum number of hardware is properly
clients (32) may already installed.
be registered in the
driver.
0x0056 (WIN_CAN_CONNECT_TO_NET) Error connecting to CAN Make sure that the CAN
net. hardware is properly
installed.
0x0057 (WIN_CAN_REGISTER_MSG) Error registering Make sure that the CAN

message mask. CAN hardware is properly
driver may be corrupt. installed.
0x0058 (WIN_CAN_SET_EVENT) Error setting event Make sure that the CAN
handler. Operating hardware is properly
system call failed. installed.
MSTM/2307 3-11
0x0059 (WIN_CAN_GET_CLIENT_INFO) Error getting client info. Restart program. If this does
CAN driver may be not help, then
corrupt. R. S.
0x005A (WIN_CAN_RECEIVE) Error receiving/reading Check the connection, and

CAN frame. Timeout try again. If this does not
occurs during a buffer help, then R. S.
transfer.
0x005B (WIN_CAN_TRANSMIT) Error transmitting CAN Check the connection, and

frame. try again. If this does not
Timeout occurs during a help, then R. S.
buffer transfer.
0x005C (WIN_CAN_RECEIVE_TIMEOUT) Error receiving/reading Check the connection, and

CAN frame. No CAN try again. If this does not
frame arrived within the help, then R. S.
time limit
0x005D (WIN_CAN_TRANSMIT_TIMEOUT) No CAN frame could be Check the connection, and

transmitted within the try again. If this does not
time limit. help, then R. S.
0x005E (WIN_CAN_NET_ERROR) Frame received in wrong Check CAN connection.

CAN net CAN driver may Restart program. If this does
be corrupt not help, then
R. S.
0x005F (WIN_CAN_RESET_ERROR) Error resetting client and Check CAN connection.

event handler. Restart program.
If this does not help, then
R. S.
0x0060 (WIN_CAN_REMOVE_CLIENT) Error removing client R. S.

from CAN Net.
0x0061 (WIN_CAN_SERVER_THREAD) Error creating the server Restart program.

receive thread. Operating
system call failed.
R. S.
0x0062 (WIN_CAN_CREATE_PIPE) Error creating a pipe. Restart program.

Operating system call If this does not help, then
failed. R. S.
0x0063 (WIN_CAN_CONNECT_PIPE) Error connecting to a Restart program. If this does

pipe. Operating system not help, then
call failed. R. S.
3-12 MSTM/2307
0x0064 (WIN_CAN_OPEN_PIPE) Error opening a pipe. Restart program.

Operating system call
failed.
R. S.
0x0065 (WIN_CAN_WRITE_PIPE) Error writing to pipe. Restart program. If this does

Operating system call not help, then
failed. R. S.
0x0066 (WIN_CAN_READ_PIPE) Error reading from pipe. Restart program.

failed.
R. S.
0x0067 (WIN_CAN_TRANSACT) Error during pipe Restart program. If this does

transaction.Operating not help, then
system call failed. R. S.
0x0068 (WIN_CAN_SETPARAM) Error setting pipe Restart program. If this does

parameters. Operating not help, then
Error setting pipe parameters.

system call failed. R. S.
0x006A (WIN_CAN_CREATE_MUTEX) Error creating mutex. Restart program.

failed.
R. S.
0x006B (WIN_CAN_OPEN_MUTEX) Error getting existing Restart program. If this does

mutex. Operating system not help, then<<<
call failed. R. S.
0x006C (WIN_CAN_WAIT_MUTEX) Error getting existing Restart program.

mutex. Operating system If this does not help, then
call failed. R. S.
0x006D (WIN_CAN_INVALID_FRAME) The received frame was Restart program. If this does
not valid. CAN not help, then
communication has lost R. S.
synchronisation.
0x006F (WIN_CAN_CLIENT_UNKNOWN) Unknown Client type. Restart program.
0x0070 (WIN_CAN_BUS_HEAVY) CAN-Bus Heavy. Too Check CAN connection.

many error frames sent Make sure, that at least two
by this communication CAN-Controllers are
object. connected to the CAN
network.
0x0071 (WIN_CAN_FILTER) Error setting the CAN Restart program. If this does
filter not help, then
R. S.
MSTM/2307 3-13
0x0072 (WIN_CAN_SYNC) Synchronisation error. Restart program.

Communication object If this does not help, then
has a frame in its queue R. S.
before Transmit.
0x0073 (WIN_CAN_MAX_BRIDGE) Max number of server- Restart program. If this does

bridges was not set. not help, call Eppendorf
Service
0x0074 (WIN_CAN_OVERRUN) CAN-Controller was read Restart program.

too late.
CAN communication has
lost synchronisation.
0x0075 WIN_CAN_BUS_LIGHT CAN bus Light error. Check CAN connection.

Restart program.
R. S.
0x0076 WIN_CAN_BUS_OFF CAN-Bus OFF. Too many Check CAN connection.

error frames sent by this Restart program.
communication object. If this does not help, then
R. S.
0x0077 WIN_CAN_BRIDGE_THREAD CAN bridge thread was Restart program.

not successfully created. Make sure that the CAN
installed.
0x0108 Command Error This error applies to all Notify Eppendorf Service in
building groups with Hamburg.
COMMAND Interpreter.
0x0109 Error loading program/method Too many bytes during R.S. and restart a method
the transmission run. CAN bus error. Check
indicated. patch cable, or PCB
Computer.
0x010A Error loading program/method Timeout during CAN R.S. and restart a method
communication run. CAN bus error. Check
patch cable, or PCB
Computer.
0x010E Error loading program/method CAN controller was reset. R.S. and restart a method
run. CAN bus error. Check
patch cable, or PCB
Computer.
3-14 MSTM/2307
0x010F Error loading program/method Communication was R.S. and restart a method
broken off. run. CAN bus error. Check
patch cable, or PCB
Computer.
0x0111 Error loading program Load RAM Error during Shutdown and switch off the
slave-programming instrument; if error re-occurs
after switching on and re-
starting a method run:
Change Slave-device
Notify Eppendorf Service in
Hamburg.
0x0112 Error loading program/method No proxy present. R.S. and restart a method
run. Notify Eppendorf Service
in Hamburg.
0x0113 Error loading program/method No more memory. R.S. and restart a method
in Hamburg.

0x0114 Error loading program/method CAN object missing. Notify Eppendorf Service in
Hamburg.
0x0116 Error loading program/method CAN protocol error. R.S. and restart a method
in Hamburg.
0x0140 Mouse Error The mouse is not Check or replace mouse.

recognized. Notify Eppendorf Service in
Hamburg.
0x0200 I2C Error I2C Error Notify Eppendorf Service in

Hamburg.
0x0201 I2C Struct Error I2C Struct Error. Notify Eppendorf Service in
Hamburg.
0x0202 I2C Write Error Write error on the Notify Eppendorf Service in
I2C-bus. Hamburg.
0x0203 I2C Read Error Read error on the Notify Eppendorf Service in
I2C-bus. Hamburg.
0x0204 I2C 2401_1 Error in 24C01 bus Notify Eppendorf Service in

Error 1. Hamburg.
0x0205 I2C 24C01 Read Read error on the Notify Eppendorf Service in
I2C-bus. Hamburg.
0x0206 I2C 24C01-Write Write error on the Notify Eppendorf Service in

I2C-bus. Hamburg.
MSTM/2307 3-15
0x0207 I2C 24C65 Bus error. Notify Eppendorf Service in

Hamburg.
0x0208 I2C Error Device Component is missing or Notify Eppendorf Service in

defective. Hamburg.
0x0209 I2C Error SCL SCL ist zu lange auf low. Disconnect all units after
each other, from I²C-Bus.
Notify Eppendorf Service in
Hamburg.
0x0210 I2C_CLK_WRITE Write error in clock R.S. Notify Eppendorf

device 1307 on Service in Hamburg.
PCB CPU.
0x0211 I2C_CLK_READ Read error in clock R.S. and check or replace

device 1307 on PCB Computer.
PCB CPU.
0x0212 I2C_CLK_ERROR_DEVICE Clock device 1307 on R.S. and check or replace

PCB CPU is missing or PCB Computer.
defective.
0x0215 I2C_EECPU_WRITE Write error in EEprom R.S. and check or replace

24C64 on PCB CPU. PCB Computer.
0x0216 I2C_EECPU_READ Read error in EEprom R.S. and check or replace

24C64 on PCB CPU. PCB Computer.
0x0217 I2C_EECPU_ ERROR_DEVICE EEprom 24C64 on PCB R.S. and check or replace
CPU is missing or PCB Computer.
defective.
0x0220 I2C_EEHF_WRITE Write error in EEprom R.S. and replace

24C01 on heatfoil. Mastercycler ep realplex
module heated frame or PCB
Control.
0x0221 I2C_EEHF_READ Read error in EEprom R.S. and replace

24C01 on heatfoil. Mastercycler ep realplex
Control.
0x0222 I2C_EEHF_ ERROR_DEVICE EEprom 24C01 on R.S. and replace

heatfoil is missing or Mastercycler ep realplex
defective. module heated frame or PCB
Control.
0x0225 I2C_SE1HF_WRITE Write error in R.S. and replace

temperature sensor 1 Mastercycler ep realplex
on heatfoil. module heated frame or PCB
Control.
3-16 MSTM/2307
0x0226 I2C_SE1HF_READ Read error in R.S. and replace

Control.
0x0227 I2C_SE1HF_ ERROR_DEVICE Temperature sensor 1 R.S. and replace

on heatfoil is missing or Mastercycler ep realplex
Control.
0x0230 I2C_SE2HF_WRITE Write error in R.S. and replace

Control.
0x0231 I2C_SE2HF_READ Read error in R.S. and replace

Control.

0x0232 I2C_SE2HF_ ERROR_DEVICE Temperature sensor 2 R.S. and replace
on heatfoil is missing or Mastercycler ep realplex
Control.
0x0235 I2C_EEHK_WRITE Write error in EEprom R.S. and replace

24C64 on PCB Block. thermoblock.
0x0236 I2C_EEHK_READ Read error in EEprom R.S. and replace

24C64 on PCB Block. thermoblock.
0x0237 I2C_EEHK_ ERROR_DEVICE EEprom 24C64 on PCB R.S. and replace

Block is missing or thermoblock.
defective.
0x0240 I2C_SEKK_WRITE Write error in R.S. and replace

temperature sensor thermoblock.
TC74 on cooler.
0x0241 I2C_SEKK_READ Write error in R.S. and replace

temperature sensor thermoblock.
TC74 on cooler.
0x0242 I2C_SEKK_ERROR_DEVICE Temperature sensor R.S. and replace

TC74 on cooler is thermoblock.
missing or defective.
0x0245 I2C_EEANL_WRITE Write error in EEprom R.S. and replace PCB

24C01 on PCB Launch. Launch.
0x0246 I2C_EEANL_READ Read error in EEprom R.S. and replace PCB

24C01 on PCB Launch. Launch.
MSTM/1808 3-17
0x0247 I2C_EEANL_ERROR_DEVICE EEprom 24C01 on PCB R.S. and replace PCB

Launch is missing or Launch.
defective.
0x0250 I2C_EELE_WRITE Write error in EEprom R.S. and replace PCB Power
24C01 on PCB Power. unit .
0x0251 I2C_EELE_READ Read error in EEprom R.S. and replacePCB Power

24C01 on PCB Power. unit .
0x0252 I2C_EELE_ERROR_DEVICE EEprom 24C01 on R.S. and replace PCB Power

PCB Power is missing unit .
or defective.
0x0700 FLASH-Timeout Timeout Flashloader. Replace PCB Computer.
0x0701 FLASH-Err-Erase Second gate could not Replace PCB Panel or PCB
be deleted. Computer.
0x0702 FLASH-Err-Prog Programming error. Replace PCB Panel or PCB

Computer.
0x0703 FLASH-Err Software does not work. Replace PCB Panel or PCB
Computer.
0x0704 FLASH-Err-Memory The software is too large Replace PCB Panel or PCB
for the flash. Computer.
0x0705 FLASH-Err-Flash Flash not programmed. Replace PCB Panel or PCB

Unknown quantity flash Computer.
type.
0x0706 FLASH-Err-Typ In the Flash is no valid Replace PCB Computer.

program.
0x0709 (FLASH_ERR_CORRUPT) File may have been Obtain new file.

The file to be flashed contains corrupted during
defective control information. copying.
0x070A (FLASH_ERR_CRC16) File may have been Obtain new file.

The checksum across a certain area corrupted during
of a file to be flashed is wrong. copying.
0x070B (FLASH_ERR_FILE_EMPTY) File may have been Obtain new file.

The file to be flashed is empty. corrupted during
copying.
0x0A00 STR-Timeout Software error. R.S.
0x0A01 STR-Netz-Error Power-Net is not correct. Select the right voltage

version.
3-18 MSTM/1808
0x0B00 (FS_TIMEOUT ) Timeout between file Try command again if

Communication error in file system. system proxy and server. possible, otherwise reboot
entire system.
0x0B01 (FS_INVALID_CMD) Command sent to the file R.S.

Invalid Command. system is invalid. Check CAN-Bus, Panel und
Patch cabel.
0x0B03 (FS_ERR_NOT_IMPLEMENTED) Function has not yet Notify Eppendorf Service in

Function not yet implemented been implemented Hamburg.
0x0B05 (FS_ERR_PATH) Internal file path Operator error:

Error reading file path. conversion error. Make sure that the file name
and path is valid.
0x0B06 (FS_ERR_DAMAGE) File system has been R.S.

Filesystem damaged. damaged.
0x0B08 (FS_ERR_INVALPATH) Filename or path is Make sure that the file name

Invalid path or filename. invalid. and path is valid.
0x0B09 (FS_ERR_XFILESOPEN) The number of allowed Operator error:

Too many Files/Directories open. open files and directories Close other open files.
has reached its
maximum.
0x0B0A (FS_ERR_DOES_NOT_EXIST) File or directory does not Operator error:

File or directory does not exist. exist. Make sure that the file name
and path is valid.
0x0B0B (FS_ERR_NONAME) Software error. R.S.

No name or directory found.
0x0B0C (FS_ERR_NOT_OPEN) Software error. R.S.

Could not open file.
0x0B0D (FS_ERR_OPEN) File may be in use. Make sure that the file is not
Error opening file or directory. in use and try to open it
again.
0x0B12 (FS_ERR_BADFILEID) Software error. R.S.

File ID invalid.
0x0B14 (FS_ERR_IN_USE) See error message. Make sure that file is not in
File is in use and cannot be use any more and try again.
accessed.
0x0B42 (FS_ERR_READING) File may be corrupted. R.S.

Error reading file.
0x0B44 (FS_ERR_ILL_FILE_LENGTH) File may be corrupted. R.S.

Illegal file length. Trying to read or
write beyond file.
MSTM/1808 3-19
0x0B48 (FS_ERR_FILE_EXISTS) See error message. Use another name for the
Error creating file. File exists. new file. User error.
0x0B4B (FS_ERR_IS_DIR) Software error. R.S.

Entry is not a file, but a directory.
0x0B81 (FS_ERR_DIR_EXISTS) See error message. Use another name for the
Error making directory. Directory new file. User error.
exists!
0x0B85 (FS_ERR_FILES_MISSING) Some files may be R.S.

Error listing files. Number of files in deleted, or directory is
directory is not the same anymore. corrupt.
0x0B87 (FS_ERR_DIR_NOT_EMPTY) Software error. Operator error:

Entry is not a file, but a directory. R.S.
0x0B8D (FS_ERR_NOT_DIR) Software error. Operator error:

Entry is not a directory. R.S.
0x0BC0 (FS_ERR_FORMAT_ABORTED) See error message. Message for user.

Format aborted by user.
0x0D01 TCYC_CHKSUM_ERR_CPU Checksum-error on R.S. and check or replace

PCB CPU. PCB Computer.
0x0D02 TCYC_CHKSUM_ERR_LEISTUNG Checksum-error on R.S. and check or replace

PCB Power. PCB Power unit.
0x0D03 TCYC_CHKSUM_ERR_ANLAUF Checksum-error on R.S. and check or replace

PCB Launch. PCB Launch.
0x0D04 TCYC_CHKSUM_ERR_HEIZKRAGE Checksum-error on R.S. and replace

N PCB Block. thermoblock.
0x0D05 TCYC_CHKSUM_ERR_HEIZFOLIE Checksum-error on R.S. and replace

heatfoil. Mastercycler ep realplex
Control.
0x0D07 TCYC_FUSE_ERR General fuse error. R.S. and check fuses or

replace PCB Power unit.
0x0D08 TCYC_FUSE_ERR_AD_45V Voltage 45V is missing, R.S. and check fuses or

F901 defective. replace PCB Power unit.
0x0D09 TCYC_FUSE_ERR_AD_VSUB Voltage VSUB is missing, R.S. and check fuses or

0x0D0A TCYC_FUSE_ERR_AD_UREF1_25V Voltage UREF1_25V is R.S. and check fuses or

missing. replace PCB Power unit.
3-20 MSTM/1808
0x0D0B TCYC_FUSE_ERR_AD_V_PELT_1 Voltage PELT_1 is R.S. and check fuses or

missing, F902 defective. replace PCB Power unit.
0x0D0C TCYC_FUSE_ERR_AD_V_PELT_2 Voltage PELT_2 is R.S. and check fuses or

0x0D0D TCYC_FUSE_ERR_AD_V_PELT_3 Voltage PELT_3 is R.S. and check fuses or

0x0D0E TCYC_FUSE_ERR_AD_30V_AUX Voltage 30 V is missing, R.S. and check fuses or

0x0D10 I2C_ERROR_1 Error on I2C-bus 1. R.S. and replace PCB

Computer.
0x0D11 I2C_RESOURCE_ERROR_1 Software error on R.S. and replace PCB

I2C-bus 1. Computer.
0x0D12 I2C_ERROR_2 Error on I2C-bus 2. R.S. Notify Eppendorf

Service in Hamburg.
0x0D13 I2C_RESOURCE_ERROR_2 Software error on R.S. Notify Eppendorf

I2C-bus 2. Service in Hamburg.
0x0D16 TCYC_SPI_ERROR Error on SPI-bus. R.S. and replace PCB

Launch or PCB Power unit.
0x0D17 TCYC_SPI_RESOURCE_ERROR Software error on R.S. and replace PCB

SPI-bus. Launch or PCB Power unit.
0x0D18 TCYC_SNT_OVERTEMP SNT has detected an R.S. and check or replace

over-temperature. power supply or fan.
0x0D19 TCYC_SNT_POWERFAIL SNT has detected a R.S. and check or replace

powerfail. power supply.
0x0D1C TCYC_FAN_START_ERROR Fan did not start. R.S. and check or replace fan
or PCB Power unit.
0x0D1D TCYC_PRN_INIT_ERROR Error on printer R.S. and check or replace

initialisation. PCB Computer or check
printer.
0x0D1E TCYC_PRN_PRINT_ERROR Error on accessing the R.S. and check or replace

printer. PCB Computer or check
printer.
0x0f00 (TRVERR_NEWNAME_NOTAVAILAB All available default Operator error:

LE) names for new nodes Delete nodes or re-name
No more user nodes available. (e.g., "user00" to nodes so that default names
"user99") are already in are free.
use.
MSTM/1808 3-21
0x0D25 TCYC_TESTO_INITERROR Error in connecting the R.S. and use zero-modem

external measurement adapter or replace PCB
system. Computer or check validation
system.
0x0D26 TCYC_TESTO_MEASERROR Error in external R.S. and replace PCB

measurement. Computer or check validation
system.
0x0D29 TCYC_LID_OVERTEMP Over-temperature R.S. and replace

detected on heatfoil. Mastercycler ep realplex
Control.
0x0D2A TCYC_LID_ERROR General error in lid R.S. and replace

control. Mastercycler ep realplex
Control.
0x0D2B TCYC_LID_DIFFERROR_T1
Error in temperature R.S. and replace

difference in sensor 1 on Mastercycler ep realplex
the heatfoil. module heated frame or PCB
Control.
0x0D2C TCYC_LID_DIFFERROR_T2 Error in temperature R.S. and replace

difference in sensor 2 on Mastercycler ep realplex
Control.
0x0D2D TCYC_LID_DIFFERROR Difference between both R.S. and replace

temperature sensors is Mastercycler ep realplex
too big. module heated frame or PCB
Control.
0x0D2E TCYC_LID_RISEERROR Lid control detected too R.S. and check or replace
small rising. PCB Launch.
0x0D2F TCYC_LID_STEMPNOTREACHED Lid control did not reach R.S. and check or replace
target value. PCB Launch.
0x0D31 TCYC_INIT_FAIL_LID Error in initialisation of R.S. and replace

the lid control. Mastercycler ep realplex
Control.
0x0D40 TCYC_ESPI Error in SPI R.S. and replace PCB Power

communication. unit or PCB Computer or
PCB Launch, one after the
other
0x0D42 TCYC_ETEMPLO Under-temperature R.S. and replace PCB

detected by ADC. Launch.
3-22 MSTM/1808
0x0D43 TCYC_ETEMPHI Over-temperature R.S. and replace PCB

detected by ADC. Launch.
0x0D50 TCYC_TREG_ERRHI Blockcontrol detected R.S. and replace

over-temperature. thermoblock. or PCB Launch,
one after the other.
0x0D51 TCYC_TREG_ERRLO Blockcontrol detected R.S. and replace

under-temperature. thermoblock. or PCB Launch,
one after the other.
0x0D52 TCYC_TREG_ADCERR Blockcontrol detected R.S. and replace PCB

error in ADC Launch or PCB Power unit or
communication. PCB Computer, one after the
other.
0x0D53 TCYC_TREG_DACERR Blockcontrol detected R.S. and replace PCB Power

error in DAC unit or PCB Computer, one
communication. after the other.

0x0D70 TCYC_FAN_COOLEROVERTEMP The cooler got too hot. R.S. and check ventilation /
fan or ambient temperature
or replace thermoblock.
0x0D90 TCYC_LID_SENSOR1ERR Error in reading the R.S. and replace

temperature sensor 1 on Mastercycler ep realplex
Control.
0x0D91 TCYC_LID_SENSOR2ERR Error in reading the R.S. and replace

temperature sensor 2 on Mastercycler ep realplex
Control.
0x0DDC0 Communication failure R.S.
0x0DF0 TCYC_WRONGVOLTAGE Wrong input voltage. R.S. and check voltage

version in service function.
0x0F00 (TRVERR_NEWNAME_NOTAVAILAB All available default Delete nodes or rename

LE) names for new nodes nodes so that default names
No more user nodes available. (e.g., "user00" to are free. User error.
"user99") are already in
use.
0x0F01 (TRVERR_NEWNODE_NOTAVAILAB The maximum number of Delete all nodes that are no
LE) nodes (1000) are in use longer needed. User error.
No more nodes available. already.
0x1001 ILLEGAL_LED Illegal LED specified Notify Eppendorf Service in

Hamburg.
MSTM/1808 3-23
0x1002 ILLEGAL_CHANNEL Illegal channel Notify Eppendorf Service in

Hamburg.
0x1003 UNKNOWN_SWITCH Unknown switch Notify Eppendorf Service in

Hamburg.
0x1004 ILLEGAL_FILTER Unknown filter No. Filter coding. Check light

barrier.
0x1005 NULLPOS_NOT_FOUND Null position on filter Filter coding. Light barrier.

wheel not found Filter wheel.
0x1006 FA_NO_END Light barrier always Filter coding. Check light

open. barrier.
0x1007 FA_ALWAYS_END Light barrier always Filter coding. Check light

closed barrier.
0x1008 FA_NO_CODE No filter coding found on Filter coding. Check light

a filter wheel barrier.
0x1009 FA_WRONG_CODE A filter code was found Filter coding. Check light
but could not be barrier.
decoded
0x100A SPI_BUS_BUSY Conflict with access to R.S.

SPI bus.
0x100B RAMP_TOO_LONG Ramp error Notify Eppendorf Service in

Hamburg.
0x100C NO_RAMP_CALC Ramp could not be Notify Eppendorf Service in

calculated Hamburg.
0x100D ILLEGAL_POTIVALUE Potentiometer value Notify Eppendorf Service in

outside [0..255] Hamburg.
0x100E NO_LOGFILESERVER Filesys server not Notify Eppendorf Service in

functioning Hamburg.
0x1013 NOT_IN_SERVICE A function may only be Notify Eppendorf Service in

called up in service Hamburg.
mode
0x1014 FAN_FAILURE Fan not functioning Fan; Replace PCB Control.

properly.
0x1015 CAL_BEREICH_UNTEN Range not observed Error can only occur on

during calibration. startup, see Service Manual
section 3
"Commissioning Software".
3-24 MSTM/1808
0x1016 CAL_BEREICH_OBEN Excessive variance on Error can only occur on

calibration. startup, see Service Manual
section 3
"Commissioning Software"..
0x1018 CAL_ABWEICHUNG Sensitivity deviation too Error can only occur on

high on calibration. startup, see Service Manual
section 3
"Commissioning Software"..
0x1019 CPM_TOO_LOW No adequate signal Notify Eppendorf Service in

found for sensitivity Hamburg.
correction
0x101A LED_FEHLER LED defective. Replace PCB LED matrix.
0x101B ILLEGAL_VOLTAGE An illegal voltage was Multiplier too insensitively.

set. Replace PMT.

0x101C OD_REFLED ERROR Reference LED defective!
Replace PCB LED Matrix
0x1024 PMT_A_DEFEKT The Multiplier A / 650nm Check the Multiplier with the
is reported as defective . RealSpector and evaluate the
log file.
0x1025 PMT_B_DEFEKT The Multiplier B / 900nm Check the Multiplier with the
is reported as defective. RealSpector and evaluate the
log file.
0x1027 CMX_TIMEOUT_FA1_TX Internal communication Check filter drum 1 drive and

error with filter drive 1. light barrier.


0x102A CMX_TIMEOUT_FA2_TX Internal communication Check filter drum 2 drive and

0x1200 (ABLS_INVALID_CMD) Software error. R.S.

Invalid Command number. Check CAN-Bus, Panel und
Patch cabel.
0x1204 (ABLS_NO_PROGRAM) Software error. R.S.

No Program loaded.
0x1205 (ABLS_NO_SPACE) Heap is full. R.S. Notify Eppendorf

Not enough memory to allocate Service in Hamburg.
buffer.
MSTM/1808 3-25
0x120A (ABL_INTENTIONALLY_STOPPED) User pressed the "Abort" Message for user.

Program aborted by user. button during program
run.
0x120D (ABL_ABLTIMEOUT) CAN communication lost R.S. Notify Eppendorf

"Process control task" is not synchronisation. Service in Hamburg.
responding.
0x120E (ABL_ABLTASKTIMEOUT) CMX message timeout R.S. Notify Eppendorf

"Process task" is not responding. between proxy and Service in Hamburg.
server.
0x1212 (ABL_LOGSPOOLTIMEOUT) CMX message timeout R.S. Notify Eppendorf

"Log spooling" is not responding. between proxy and Service in Hamburg.
server.
0x1217 (ABL_WRONG_LOGTYPE) Software error. R.S.

Wrong log type.
0x1218 (ABL_LOAD_ERR_WRONG_STATE) Software error. R.S.

Load error. Process state does not
allow this action.
0x1219 (ABL_START_ERR_WRONG_STATE) Software error. R.S.

Start error. Process state does not
allow this.
0x121A (ABL_STOP_ERR_WRONG_STATE) Software error. R.S.

Stop error. Process state does not
allow this.
0x121B (ABL_CONTINUE_ERR_WRONG_ST Software error. R.S.

ATE)
Continue error. Process state does
not allow this.
0x121C (ABL_SNGL_STEP_ERR_WRONG_S Software error. R.S.

TATE)
Single step error. Process state
does not allow this.
0x121D (ABL_ABORT_ERR_WRONG_STATE) Software error. R.S.

Abort error. Process state does not
allow this.
0x121E (ABL_INIT_ERR_WRONG_STATE) Software error. R.S.

Initialisation error. Process state
does not allow this.
3-26 MSTM/1808
0x121F (ABL_LOGTIMEOUT) CMX message timeout Check Control panel and

Process log server not responding. between Proxy and PCB Computer
server. R.S. and try again. If this
does not help, then notify
Eppendorf Service in
Hamburg.
0x1220 (ABL_INTERNAL_ERROR) Software error. R.S.

Internal error has caused the
system to stop, while running.
0x1221 (ABL_HOOD_WAS_OPEN) See error message. Close realplex module.

The Hood was opened while the
program was stopped.
0x1222 (ABL_TRANSFER_DISABLED) See error message. Start program again.

Transfer allowance was prematurely
deactivated during program
initialisation.

0x1241 (ABL_UTIL_WRONG_WAIT_STATE) ”Wait_STime()” has been R.S. and and try again. If this
Illegal wait state used. called with a wrong wait does not help, then notify
state. Eppendorf Service in
Hamburg.
0x1245 (ABL_TEMP_TOO_HIGH) Illegal value has been Check temperature in

Block temperature in program is too detected in program. program editor and start
high. program again. User error.
0x1246 (ABL_TEMP_TOO_LOW Illegal value has been Check temperature in

Block temperature in program is too detected in program. program editor and start
low. program again. User error.
0x1247 (ABL_TEMPINC_TOO_HIGH) Illegal value has been Check temperature in

Block temperature increment in detected in program. program editor and start
program program again. User error.
is too high.
0x1248 (ABL_TEMPINC_TOO_LOW) Illegal value has been Check temperature in

Block temperature increment in detected in program. program editor and start
program program again. User error.
is too low.
0x1249 (ABL_STEPTIME_TOO_HIGH) Illegal value has been Check step time in program
Step time in program is too high. detected in program. editor and start program
again. User error.
0x124A (ABL_STEPTIME_TOO_LOW) Illegal value has been Check step time in program
Step time in program is too low. detected in program. editor and start program
again. User error.
0x124B (ABL_TIMEINC_TOO_HIGH) Illegal value has been Check time increment in

Time increment in program is too detected in program. program editor and start
high. program again. User error.
MSTM/1808 3-27
0x124C (ABL_TIMEINC_TOO_LOW) Illegal value has been Check time increment in

Time increment in program is too detected in program. program editor and start
low. program again. User error.
0x124D (ABL_TOO_MANY_CYCLES) Illegal value has been Check value in program

Too many cycles in Cycle command. detected in program. editor and start program
again. User error.
0x124E (ABL_NOT_ENOUGH_CYCLES) Illegal value has been Check value in program

Not enough cycles in Cycle detected in program. editor and start program
command. again. User error.
0x124F (ABL_TOO_MANY_STEPS) Illegal value has been Check value in program

Too many steps within a Cycle. detected in program. editor and start program
again. User error.
0x1250 (ABL_NOT_ENOUGH_STEPS) Illegal value has been Check value in program

Not enough steps within a Cycle. detected in program. editor and start program
again. User error.
0x1251 (ABL_RAMP_TOO_HIGH) Illegal value has been Check value in program

Tempering ramp is too high. detected in program. editor and start program
again. User error.
0x1252 (ABL_RAMP_TOO_LOW) Illegal value has been Check value in program

Tempering ramp is too low. detected in program. editor and start program
again. User error.
0x1253 (ABL_GRADIENT_TOO_HIGH) Illegal value has been Check value in program

Gradient is too high. (out of limits). detected in program editor and start program
again. User error.
0x1254 (ABL_LID_TIMEOUT) Block regulator at R.S. and replace

The Lid has not reached the wanted theframe with heating foil Mastercycler ep realplex
temperature in time. may be defective or not module heated frame or PCB
properly calibrated. Control.
0x1255 (ABL_BLOCK_TIMEOUT_L) Block regulator may be Try validation, otherwise

The Left Block has not reached the defective or not properly replace thermoblock or PCB
wanted temperature in time. calibrated. Power unit.
0x1256 (ABL_BLOCK_TIMEOUT_M) Block regulator may be Try validation, otherwise

The Middle Block has not reached defective or not properly replace thermoblock or PCB
the wanted temperature in time. calibrated. Power unit.
0x1257 (ABL_BLOCK_TIMEOUT_R) Block regulator may be Try validation, otherwise

The Right Block has not reached the defective or not properly replace thermoblock or PCB
wanted temperature in time. calibrated. Power unit.
0x1258 (ABL_WRONG_BLOCKTYPE) Illegal value has been Check block type in program
The wanted block type does not detected in program. editor and start program
correspond with that of the cycler. again. User error.
3-28 MSTM/1808
0x125A (ABL_PROGRAM_TOO_BIG) Program cannot be Try to make the program

The loaded program is too big loaded into memory shorter.
(exceeds 16Kb). because it is too big.
0x1602 (CE_ERR_PROG_TOOLONG) Maximum size of steps Delete commands that are

Program too long. (100) has been reached. not necessary before
inserting new commands.
Could be a user error.
0x2600 (PRN_INIT_ERROR) Error initialising Printer. Check printer connection

cables.
R.S.
0x2601 (PRN_PRINT_ERROR) Error sending a file to the R.S.

printer.
0x2602 (PRN_BUFFER_VOLL) Printer buffer is full. Restart program. R.S.
0x2603 (PRN_STATUS_BUSY) Printer is busy or offline. Printer may be printing too

slowly. Restart program.
R.S.
If this doesn't work either,
then try another printer.
0x2604 PRN_STATUS_PAPEROUT Printer is out of paper. Add some paper
0x2605 (PRN_STATUS_FAIL) System could not get a R.S.

proper status.
0x2606 (PRN_I2C_ERROR) Printer encountered an R.S.

I2C Error.
0x2607 (PRN_I2C_RESOURCE) Printer I2C resource Restart program. R.S.

error. If this doesn't work either call
Eppendorf service in
Hamburg.
0x2609 (PRN_INIT_FAIL) Error initialising printer R.S.

"escape sequence".
0x260A (PRN_INIT_NO_DEFAULT) No default initialisation Make sure that the file at :

values available. /top/system/printer/printer.ini
is not corrupt.
0x260B (PRN_BUFFER_SENT_IS_EMPTY) Buffer sent to printer is R.S.

empty.
0x260C (PRN_BUFFER_TOO_BIG. Buffer sent to printer is R.S.

too big.
0x260D (PRN_NOT_ENOUGH_MEMORY) Not enough memory to R.S.

allocate buffer.
MSTM/1808 3-29
0x260E (PRN_OFFLINE) Printer is offline. Make sure that printer is

online.
0x260F (PRN_TIMEOUT) CMX Timeout between R.S.

printer proxy and server.
0x010001 OD_LED_FAILURE One or more LEDs are Replace PCB LED Matrix.
defective.
0x010002 OD_REF_FAILURE Reference _LED defect Replace PCB LED Matrix.
0x010003 OD_PMT_OUTOFRANGE Dark signal outside Ceck multiplier/PCB

permissible limits. amplifier. Avoid stray light.
0x010004 OD_PMT_VON_LOW Dark signal too weak Multiplier/PCB Amplifier/PCB

with voltage on. Control
0x010005 OD_PMT_VON_HIGH Dark signal too strong Ceck multiplier/PCB

with voltage on. amplifier. Avoid stray light.
0x010006 OD_PMT_VOFF_LOW Dark signal too weak Ceck multiplier/PCB

with voltage off. amplifier. Avoid stray light.
0x010007 OD_PMT_VOFF_HIGH Dark signal too strong Ceck multiplier/PCB

with voltage off. amplifier. Avoid stray light.
0x010008 OD_PMT_COMPARISON Dark signal not increased May be OK at 650 nm

with PMT voltage. multiplier, this then involves a
message and not an error
message. Otherwise PCB
amplifier. Ceck PCB Control.
0x010009 OD_OPTIC Optical self-test: raw If a black test plate is

values too great. present, the thermoblock can
be ruled out for this error.
One or more "light paths" are
fluorescent.
0x01000A OD_FILTER Error on initialization of a Filter coding, light barrier,

filter wheel. motor for filter wheel. Ceck
PCB Control.
0x01000B OD_LED_LOW LED(s) fall short of stray Lumogen plate is possibly

light tolerance. defective. Soiling in light path
(not fluorescent) LEDs
outside specification.
Replace PCB Matrix.
0x01000C OD_LED_HIGH LED(s) exceed stray light Lumogen plate is possibly

tolerance. defective. Soiling in light path
(fluorescent) LEDs outside
specification. Replace PCB
Matrix.
3-30 MSTM/1808
0x01000D OD_LED_LOWHIGH LED(s) fall short of and Lumogen plate is possibly

exceed stray light defective. Replace PCB
tolerance. Matrix.
0x01000E OD_LED_MEAN The middle value of the Lumogen plate is possibly

LED deviates to much defective. Replace PCB
from calibrated nominal Matrix.
value
MSTM/1808 3-31
Differentiating feature of service functions within realplex software and commissioning software.
The service functions for the realplex module/Mastercycler ep are in the realplex software. These functions can be used
for testing.
The commissioning software needs to be installed on the realplex computer separately.
It is required only in the event of the realplex being serviced or repaired, for example for switching voltage or after
replacing a PCB or a multiplier.
realplex Application Software V1.5: Call up the service functions of realplex or Mastercycler.
Conditions
Connect the computer to the Mastercycler ep realplex via the USB port, the USB CAN adapter.
– Exit all other programs.

– Only start the realplex program now.
– Switch on Mastercycler ep realplex-system.
– Select "Service" as the user name and confirm with Enter.
– Enter the password "23456789" and confirm with Enter.
– In the top program bar, call up the navigation tree via the "Setup" and "System configuration" menus.
– Open the "realplex module"/"Cycler" node by clicking on it.
– The "realplex service"/"Cycler service" functions can be found under the relevant menu.
3-32 MSTM/1808
Service functions of the realplex module from firmware version 3.36 onwards
System Info
System information is adopted on commissioning
and written to the appropriate fields.
The possible range for "Current PMT Voltage Chn." is
between 0 and 255. Each channel can be tested in
stages 0 – 9.

Filterwheels
This function can be used to perform a complete filter
drum self-test.
A 360° rotation of the filter drum is 115,200
microsteps. The offset means the deviation from the
zero position.
Each filter has its own code.
2 = 605 nm
4 = dark filter
5 = 550 nm
10 = 580 nm
15 = 520 nm
The two slide controllers at the bottom of the function
can be used to rotate the relevant filter drum into any
desired position.
If a dot can be seen after "Light Barrier", there is a
coding initiator switch for a filter in the light barrier.
MSTM/1808 3-33
LED-Array
The "Flash" button can be used to sub-ject all the
LEDs on the PCB LED Matrix to a visual inspection.
The wavelength of the LEDs is 470 nm. It is not
necessary to wear safety glasses.
With the self-test, all LEDs are tested and the result
written into the table.
0 means OK.
A red cross means that the LED in the relevant
position is defective. If one LED is defective, the PCB
LED Matrix will have to be replaced.
A repair is then performed with the aid of the
commissioning software, followed by dye calibration.
Multiplier
A semi-skirted/black plate from test box 6302
875.004 is inserted for the multiplier test.

For this dark current measurement, multiplier voltage
is first switched off (Voltage off) and then on (Voltage
on). On a multiplier which measures in the "blue
range" (520 – 550 nm), the difference between
"Voltage off" and "Volt-age on" is very small.
"Voltage on" is always slightly higher. On a multiplier
which measures in the "red range" (580 – 605), the
"Voltage on" value is much higher because it
includes the effect of heat in the infrared range. If the
upward limit value is exceeded, there is an error
message.
3-34 MSTM/1808
Optical Path
Depending on the features of the device, this
function can be used to test the relevant channels.
Each channel can be tested individually using
"Channel & Filter Selection".
The semi-skirted/black plate from test box 6302
875.004 needs to be inserted for this.
All 96 measuring positions are shown in the matrix
with figures.
0 – 4 is OK.
From 5, the range is exceeded and a red 5 appears.
If a yellow 5 can be seen, this means that a fault has
been found at this position on another channel.
Calibration
In order to verify calibration/ adjustment, the
Lumogen reference plate from test box

6302 875.004 needs to be inserted.
Under "defective wells" is a display of how many
positions are too low and how many are too high.
Too low means that the relevant light conductor is
defective or there is contamination of a lens array in
the light path. Too high means that there is contami-
nation. The mean values are shown under "mean".
Percentage details shown under "(dev.)" have been
calculated from the deviation of the actually
measured values to the stored calibration data.
Deviation may not exceed ±10 %.
Well State
For the Well State test, the Lumogen reference plate
from test box 6302 875.004 must be inserted.
Each channel can be tested individually using
"Channel & Filter Selection".
All 96 measuring positions are shown in the matrix.
In principle, this function does the same as
calibration, just showing it differently.
MSTM/1808 3-35
Logging
The Logging menu collates all the activities and they
can be backed up.
3-36 MSTM/1808
Service functions of the Mastercycler from firmware version 2.601 onwards
Firmware
This menu displays the serial number of the
Mastercycler.
This is followed by the serial number of the
Thermoblock.
CPU: PCB Computer
Power : PCB Power unit
Launch: PCB Launch
The software version of the Mastercycler is shown
after "Thermomodule".

Hardware
In field "Voltage" setting of the voltage is displayed.
The voltage can be switched in "Commissing
software" only.
"Birth" means the date the Mastercycler was
manufactured.
The temperature changes of the Thermoblock are
shown in the "Cycler Count" field.
"Impulse Count" indicates how often the impulse
process has been used. This is only possible on
devices with a silver block.
"Last Calibration"/"Last Validation" displays the date
when the last calibration was performed.
MSTM/1808 3-37
Settings
The fan under the Thermoblock can be activated with
"Voltage Fan".
Voltage values are between 12 and 25 volts.
The Thermoblock segments (left, mid, right) can
furthermore be actuated in the range from ± 0.1 – 4.0
amps.
All the Thermoblock sensors are displayed
dynamically.
Lid Drive
This function can be used to test the heating system
in the realplex module.
Range 37 °C – 105 °C.

Enter the temperature value and click in the "Heat"
field to start the function.
"Realplex lid" must appear in the "Lid Type" field.
3-38 MSTM/1808
Software update for the Mastercycler/realplex module.
Software update for the Mastercycler/ realplex

module.
The Cycler/realplex module can be updated with
these menus.

Temperature Validation/Temperature Calibration
Temperature Validation/Temperature Calibration.

These menus can be used to perform a Temperature
Validation/Temperature Calibration with the aid of
validation system 0055 000.298.
Spacers 6302 891.000 are also required. These are
positioned in the four corner wells of the
Thermoblock.
MSTM/1808 3-39
Commissioning software V1.14
Installing the realplex system

Auxiliary material: Test box 6302 875.004
Commissioning software
PC–CAN bus cable
USB CAN adapter
RJ45/RJ45 CAT 5E patch cable
Pale filter 6302 898.004
The commissioning software (IBSW) is a service test software available to download at www.eppendorf-support.com.
This commissioning software is required to adjust the realplex module.
Commissioning software version 1.14 requires Firmware V 3.36 on the PCB control.
– Connect the computer to the Cycler CAN input on the rear of the Cycler using the USB CAN adapter and the
PC–CAN bus cable.
– Connect the patch cable of Cycler CAN_out to realplex module CAN_in.
– Set the address of the Cycler to 02 using the address switch.

– Set the address of the realplex module to 11.
– Set the term switch on the Cycler in up position and on the realplex module to the down position.
– Plug the power supply cable into the Cycler and supply it with power.
– Connect the keyboard and mouse to the computer.
– When carrying out an adjustment on the realplex system, the dark filter must be replaced with a pale filter before the
device is switched on in the filter wheel.
– To do this, remove the gray cover on the realplex module.
– Unscrew both screws and remove the cover.
– Replace the dark filter with the pale filter, replace the gray cover and tighten the two screws again.
– Plug the power supply cable into the computer and supply it with power.
– Switch on the computer.
– Switch on the realplex system.
– Install the Commissioning software.
– Start the Commissioning software.
Note: only one application may ever run at a time.
3-40 MSTM/1808
– Call up Commissioning Software.

Start/Programs/Eppendorf/IBSW.
– Select the application field.
To do this, press the "Service" button.
– Press the "Synchronize" button.

– Then press the "Set data" button.
MSTM/1808 3-41
– Enter the number of the lumogen reference plate.

If components such as PCBs or multipliers are
replaced, the new serial number must be entered
with the index.
– If the pale filters have been installed?

– Press the "Yes" button.
– If this file is already available, press the "No" but-

ton. Otherwise, this message does not appear.
3-42 MSTM/1808
– If this file is already available, press the "No" but-

ton. Otherwise, this message does not appear.
– If the set mains voltage corresponds to the

mains voltage connected, press the "OK" button.
– If not, select a different mains voltage.

– Allow a warm-up time of at least 30 minutes.
Then press the "Cancel" button.
– If the lumogen reference plate has been inserted,

press the "OK" button.
MSTM/1808 3-43
– Adjustment takes approx. 30 minutes.
– Then press "Disconnect" to create an adjust-

ment protocol with the name
630xxxxxx_log_Info.txt under C:/Start/Pro-
grams/Eppendorf/IBSW.
– If a fault occurred during adjustment, send this
adjustment protocol along with a Service Info
Line (SIL) to the service department in Hamburg
for fault analysis.
– Further information on the status of the realplex

module can be gained by using the RealSpector
software. See the next page for more informa-
tion..
3-44 MSTM/1808
RealSpector Software V 1.41
Auxiliary material: Test Box 6302 875.004

The RealSpector Software is a test software for the realplex module, which can be used for testing components such as
the multiplier.
It is available to download at www.eppendorf-support.com.
– Connect the USB CAN adapter from the USB port of the computer with the Mastercycler ep realplex system.
– Switch on the Mastercycler ep realplex system and wait until the LED on the realplex module lights up green.
– Then switch on the computer.
– Double click on the RealSpector.exe to start the

program.

– Press the "Connect" button to connect the
software with the Mastercycler ep realplex
system.
MSTM/1808 3-45
– Insert the lumogen reference plate from the test

box in the thermoblock and close and lock the
realplex module.
– Then press the "Check Multiplier" button.
– When the Multiplier test is complete, a test pro-

tocol "630200410.log" is created.
3-46 MSTM/1808
– In the event of a fault, please send this protocol

together with a Service Info Line (SIL) to the ser-
vice department in Hamburg.
Technical differences between the realplex application software V1.0, V1.5 and V2.0.

Note: New parts are marked with red service numbers.
Application software: V1.0
PCB control 6302 854.007 (up to index -09)
Firmware V2.13
PCB can be upgraded to Firmware V4.02 for Multiplier PE.
Requirement: The PCB-Computer in the Mastercycler-Modul must hold the Firmware 1.714.
Commissioning software: V1.03
Adjustment utilities:
Lumogen plate 6302 875.004
Multiplier: PE
650 nm 6302 858.002
900 nm 6302 857.006
Measuring filter without red ring.

520 nm 6302 101.203
550 nm 6302 103.206
580 nm 6302 102.200
605 nm 6302 104.202
MSTM/1808 3-47
Multiplier sensitivity for E1: 8000 counts.
Application software: V1.5

PCB control 6302 854.007 (up to index -12)
Firmware V3.36
PCB can be upgraded to Firmware V4.02 for Multiplier PE.
Commissioning software: V1.14
Multiplier: PE
650 nm 6302 858.002

900 nm6302 857.006
Measuring filter without red ring

520 nm 6302 101.203
550 nm 6302 103.206
580 nm 6302 102.200
605 nm 6302 104.202
Application software V2.0

PCB control 6302 860.503 (from index -14)
Firmware V4.02
Commissioning software V2.0.10
Adjustment filter 6302 863.502
Multiplier PE
3-48 MSTM/1808
650 nm 6302 858.002

900 nm 6302 857.006
Measuring filter without red ring

520 nm 6302 101.203
550 nm 6302 103.206
580 nm 6302 102.200
605 nm 6302 104.202
Multiplier HA
650 nm 6302 854.503
900 nm 6302 855.500
Measuring filter with red ring

520 nm 6302 856.506
550 nm 6302 857.502

580 nm 6302 858.509
605 nm 6302 859.505
Note:
Problems in the range of the reference cycle may occur during the upgrade of the application software V1.0 to V2.0 and
subsequent adjustment with the respective commissioning software.
To solve this problem, the reference channel carrier 6302 899.000 (incl. two additional neutral glasses with differing trans-
parency) is required.
With a realplex² from serial number 564 and realplex4 from serial number 511, this problem will probably not occur any
more.
Changing from PE to HA is not intended.
MSTM/1808 3-49

3-50
Mastercycler® ep 6302 – 4 Disassembly / Assembly
Disassembly of upper part of housing
Aux. aids: Gloves 0019 031.381

Torx screwdriver T20
Allen key M4
The workplace must comply with the ESD guidelines and have low dust levels.
– Put on gloves.
Condition:
The realplex module has been removed from the body cycler.
– Lift lever.
– Remove the two M4 socket-head screws from the lever.
– Move the lever forwards slightly and lift off.
– Unscrew the two M4 socket-head screws from both lever hubs.
Socket-head
screws
Lever
Socket-head
Lever hub
– Remove lever hubs.
MSTM/CW10/08 4-1
– Turn module upside down.

– Remove six T20 Torx screws.
*1 If a new top part of the housing is installed, a plastic rivet is inserted in this position in place of a screw.
– Use a rubber band to hold the slide bars in place.
– Hold the module by the baseplate and stand back on the slide bars.
– Lift off upper part of housing.
6 x T20 Torx
Upper part of
housing
Slide bar *1
Sleeve Ground plate
Slide bar
4 x T 20 Torx
Block with wheel Ground plate
Frame with heating foil
When assembling the lever hubs make sure that the pin is properly inserted into the hole provided for this
purpose.
4-2 MSTM/CW10/08
Hint: The frame with heating foil must be replaced together with the Insulation plate.
This required an adjustment with the Commissioning software and a colour-calibration.
Disassembly of the Frame with heating foil

Phillips screwdriver M3
Torx screwdriver T9, T10, T20
Pads 6302 896.001
The workplace must comply with ESD guidelines and have low dust levels.
– Put on gloves.
Condition:
The realplex modul has been removed from the body cycler.
Turn realplex modul upside down.
– The Frame with heating foil is fixed with 4 x T9 Torx screws. Remove screws.
– Remove 2 x T20 Torx screws from block with wheel.
– Remove the block.
– Loose 4 x T20 Torx screws from the ground plate. Move ground plate in direction of the Mastercycler, and disconnect
plugs X7 and X8 for the multiplier when folding up.
– The PCB Control is under the ground plate.
– The flex foil of the Frame with heating foil is secured with T10 Torx screw on PCB Control. Remove screw.
– Remove plug X10 from PCB Control.
– Remove the flex foil for the heating frame from the adhesive plate on the base plate. Remove adhesive pad residue.
– Remove Frame with heating foil.
– Glue on a new pad for fastening the flex foil when assembling.
Disassembly of the insulation plate

Condition:
The Frame with heating foil has been removed.
– Remove 4 x T10 Torx screws of insulation plate.
– Remover insulation plate.
MSTM/CW10/08 4-3
Disassembly of the rubber sleeve

Phillips screwdriver M2
Condition:
The Frame with heating foil and the insulation plate has been removed.
– Put on gloves.
– Move lever in forward position (locked).

– Remove 2 x 2,5 Phillips screws on each side of the rubber sleeve.
– It could be the rubber sleeve sticks on the underground surface, but it is removable without tools.
– The lower lens array in the light path will become visible.
Cleaning of the lower lens array in the light path

Argon or nitrogen
Lens tissue
Ethanol 99%
Condition:
The Frame with heating foil, insulation plate and rubber sleeve has been removed.
– Put on gloves.
Use lens tissue and a drop of ethanol to clean the lens arrays. The area can now be blown out with argon or nitro-
gen.
4-4 MSTM/CW10/08
Replacement of the PCB Control
Aux. aids: Commissioning software

Torx screwdriver T10, T20
This description applies to the RealPlex software V 1.5. This software version includes the firmware for the RealPlex
module 3.36 and for the Mastercycler module 2.601 or higher. The startup software (IBSW) is version 1.14.
If replacement of the PCB Control is necessary in case of service, all configuration data such as Device Nr., PCB versions
and multiplier types have to be re-entered. Adjustment with the IBSW is also required.
Procedure prior to installation of the PCB Control
– Synchronize system using IBSW to write all configuration data from PCB Control to IBSW.
– Then press key "Disconnect" so that the text file 630xxxxXXX_log_inf.txt is written. This file can be found stored
under C:/Programme/Eppendorf/IBSW.
– Exit IBSW.
Procedure after replacement of PCB Control
– Set address to 11.

– Term switch down.
– Switch on system.
– Should the new PCB Control be without the current firmware, use
Start/Programme/Eppendorf/epCanFlash to call up the CanFlash tool and reflash the PCB Control.
.
MSTM/CW10/08 4-5
– To do so, first select Device Type "Online module".

– Set First Dev.-Nr. to 11.
– Press "Browse" key and select file "onlinedetection.bin".
– Press Update button to reflash PCB Control.
– Then restart IBSW and synchronize system.
– Open text file 630xxxxXXX_log_inf.txt, input configuration data manually and transfer to new PCB using
"Set data" function.
– Then perform adjustment.
Firmware update of PCB Control
If the PCB Control firmware is to be updated, this is possible using Start/Programme/Eppendorf/Firmware Update.
The firmware Update Tool shows the current firmware versions.
In this case (see below) no update is required.
4-6 MSTM/CW10/08
Replacement of the PCB Amplifier
Aux. aids: Commissioning software

Torx screwdriver T10, T20
Soldering iron
Hint:
Compare index of PCB Amplifier with Commissioning software and if necessary update the index.
See section 3. “ Commissioning software”, 8.Set data.
Condition:
The realplex modul has been removed from the body cycler.
The upper of the housing has been removed.
– Remove lid from PCB Amplifier housing.

– Unsolder both multiplier cables from PCB Amplifier, see diagram PCB amplifier 6302 804.000 in section 6.
– Disconnect ribbon cable from PCB Amplifier.
– Remove 3 x T10 Torx screws from PCB Amplifier.
MSTM/CW10/08 4-7
Disassembly LED Matrix component
Aux. aids: Torx screwdriver T10, T20

– Put on gloves.
Hint:
Compare index of PCB Matrix with Commissioning software and if necessary update the index.
See section 3. “ Commissioning software”, 8. Set data.
Conditions:
The upper part of the housing has been removed.
– Turn module upside down and loosen the 4x T20 Torx screws from the ground plate. (See 4-2)
– The PCB control is under the ground plate.
– Lift the ground plate and remove plug X1 for the PCB LED Matrix from the PCB Control.
1 6 SCH21 SCH22 1 6
X7 X3 X8
1
X19 X20 SL
C C
C C
4
4
8 1
8 1
2
UP
C334 C335
NS Shielded R210 R212 R215 R217
2
9 6
3
1 1 C326
SCH20 OK20 R213 5 1
8 8 R211 R218 R216 AMPLIFIER
C220
R220
R35
IC33 R221 TLP113

X207 C28 IC34

2 10 IC28 C327
R36
82C250
R290
PCA
R222
C201
DR1 TLP113 MAX202

SPR20
IC6 1
R24
C221
C200
OK21
R223
IC29
74HCT125
C222
C202 C203
Q1 C32
C39
C224
R29
C29 C332
C227 C22 R207
K6T8016C3M
BS616LV4015
C26
C33
R208
C330
DR31
SPR32
C38
R204 LM2594
TC7662B
*
J200
D31
SPR31
IC26 IC200 R205 IC35
MP224
C329
D30
R300 HOLDER
R206 MP222
C206
C23
R301 C35
direction
SCH1
C37 C331 DR30

Switch
C27
C167_CS
C25
C34
IC20 MP218 R298

M29F160BT
C36
R3
IC25
R299 C305
C314
R225
C302
R25
74HCT02
R224 FE30 IC19 C315

FE33
C127
FE29
FE14
FE13
FE16
C304
FE7
C123
FE12
FE18
FE10
C313
FE15
C306
FE9
C228
IC31
C229 C223 C24

FE8
FE5
FE32
C311
C337
FE17
IC32
C307
C309
R23
C316
R32
R296 SPR34
74AC04
FE6
X1 14
LEDMATRIX 26 IC21
C318
FE19
R34
F1
C308
R295
FE4 LM78l05
FE31
R22
R31
IC201 X1
C110
C119
C117
C112
C104
C102
C103
C121
C120
C118
C107
C106
*
1 13 C340
C30
FE20 C338 C301

R303 C225
C317
FE1
FE2
FE3
FE11
R33
C105
C125
C126
IC30
C303
C108
C109
C116
C111
C113
C115
C114
C124
R27
74HC14
R28 IC24 C310

C204
1 X10 22 C312
R30 C31
IC22 1 4 FE28
IC23
X4
FE25
BAT1 Q2 DS1307
24C65 C226
SCH2
FE26
FE27
C21
R203
C209
C208
R202
1 5
HEATINGFOIL X49
MAX808
D20 FE22
R214
R297 IC27 20 10 1
1 4
SPR33 C336
X9 X2 FE24
R21
R20
Label FE23
1 1 FE21 R200
C333
GND
GND
GND
SHIELD
1 5
X48
SDA_2
SCL_2
LID_+
R322
LID_-
SHIELD
R226
X13
C207
R201
IC202
X11
T1 R302 R323
ROLLUP CABLE 1
X51
6
FAN TEMP.
SENSOR
C210
R294
R292
R293
R291
C211
4-8 MSTM/CW10/08
– Pull back the ribbon cable to the opening in the baseplate.

– Fix the ground plate in place with at least two screws.
– Stand the module back on the slide bars.
– Unscrew the 4x T10 Torx screws to remove the upper bridge with the fan.
– Detach the four large springs.
– Unscrew the four T10 Torx screws from the LED Matrix component assembly.
– Lift off the LED Matrix component assembly. The assembly is guided with two pins.
4 x T10 Torx bridge
Spring
Spring
PCB LED Matrix
Spring
Photoconductor (Reference LED)
Spring
Multiplier channel 1
– Adjustment of the realplex module is then carried out with the help of the commissioning software. See Ch. 3 "Com-
missioning Software".
MSTM/CW10/08 4-9
Change of multiplier module

Slotted screwdriver M4
Lens cloth
– Put on gloves.
Hint:
Compare index of Multiplier with Commissioning software and if necessary update the index.
See section 3. “ Commissioning software”, 8. Set data.
Note:The Multiplier A 650 nm/Channel 1 requires a new top part of housing.
Conditions:
– Turn module upside down and loosen the 4x T20 Torx screws from the ground plate. (See 4-2)
– Lift the ground plate and remove plugs X7 and X8 for the multiplier from the PCB Control.
– Under the PCB Control there are the fastening screws for the relevant channel an with a two-channel device, also the
screw for the beam splitter. Unscrew the 2x T20 Torx screws for the corresponding channel, see Fig. 11.
– Fold down the ground plate and fix it in place with at least two screws.
SCH21 SCH22
X7 1
X7
6
X3
1
X8
6
X8
1
X19 X20 SL
C C
C C
4
4
8 1
8 1
2
UP
C334 C335
2
9 6
3
1 1 C326
SCH20 OK20 R213 5 1
C220
R220
R35
IC33 R221 TLP113

X207 C28 IC34
C327
2 10 IC28
R36
82C250
R290
PCA
R222
C201
DR1 TLP113 MAX202

SPR20
IC6 1
R24
C221
C200
OK21
R223
IC29
74HCT125
C222
C202 C203
Q1 C32
C39
C224
R29
C29 C332
C227 C22 R207
K6T8016C3M
BS616LV4015
C26
C33
R208
C330
DR31
SPR32
C38
R204 LM2594
TC7662B
*
J200
D31
SPR31
IC26 IC200 R205 IC35
MP224
C329
D30
R300 HOLDER
R206 MP222
C206
C23
R301 C35
direction
SCH1
C37 C331 DR30

Switch
C27
C167_CS
C25
C34
IC20 MP218 R298

M29F160BT
C36
R3
IC25
R299 C305
C314
R225
C302
R25
74HCT02
R224 FE30 IC19 C315

FE33
C127
FE29
FE14
FE13
FE16
C304
FE7
C123
FE12
FE18
FE10
C313
FE15
C306
FE9
C228
IC31
C229 C223 C24

FE8
FE5
FE32
C311
C337
FE17
IC32
C307
C309
R23
C316
R32
R296 SPR34
74AC04
FE6
14 26
LEDMATRIX IC21
C318
FE19
R34
F1
C308
R295
FE4 LM78l05
FE31
R22
R31
IC201 X1
C110
C119
C117
C112
C104
C102
C103
C121
C120
C118
C107
C106
*
1 13 C340
C30
FE20 C338 C301

R303 C225
C317
FE1
FE2
FE3
FE11
R33
C105
C125
C126
IC30
C303
C108
C109
C116
C111
C113
C115
C114
C124
R27
74HC14
R28 IC24 C310

C204
1 X10 22 C312
R30 C31
IC22 1 4 FE28
IC23
X4
FE25
BAT1 Q2 DS1307
24C65 C226
SCH2
FE26
FE27
C21
R203
C209
C208
R202
1 5
HEATINGFOIL X49
MAX808
D20 FE22
R214
R297 IC27 20 10 1
1 4
SPR33 C336
X9 X2 FE24
R21
R20
Label FE23
1 1 FE21 R200
C333
GND
GND
GND
SHIELD
1 5
X48
SDA_2
SCL_2
LID_+
R322
LID_-
SHIELD
R226
X13
C207
R201
IC202
X11
T1 R302 R323
ROLLUP CABLE 1
X51
6
FAN TEMP.
SENSOR
C210
R294
R292
R293
R291
C211
4-10 MSTM/CW10/08
Tie wrap Tie wrap
2 x T20 Torx
Multiplier-
power
supply unit
Cover
amplifier-
housing
Tie wrap Multiplier channel 1
Multiplier spring
Multiplier channel 2
– Cut open relevant tie wrap.
– Multiplier spring remove 1x T20 Torx screw with washer. The multiplier spring is no longer necessary when a new
multiplier module is to be used. However, the T20 Torx screw for the spring is once again screwed into the threads in
order to prevent the incidence of stray light.
– Unscrew one of the two screws for the relevant power supply unit of the multiplier; the other one should just be
loosened.
– Remove the cover of the Amplifier housing.
– Unsolder the relevant multiplier cable on the PCB Amplifier.
– Unscrew the seven slotted screws of the cover plate.
– Lift of cover plate.
– Place the realplex module safely to one side.
– Pull the cable for the light barrier and the filter wheel motor through the cable bushing in the direction of the multiplier
module.
– The following parts from the old multiplier module are mounted on the new module:
First the filter wheel. To this purpose loosen the M2 socket-head screw that holds the axle of the filter wheel. Turn the
filter wheel into a position until there is no filter coding flag in the light barrier.
– Pull off the filter wheel with the axle.
Important
The lights at the workstation must be dimmed so that the multiplier is not overloaded.
Check the temperature indicator on the service packaging or the multiplier.
If the indicator reads over 54°C, the new multiplier should not be used.
– Pull off the silver protective foil from the new multiplier module and check the convex lenses for dirt. Clean with a lens
cloth as necessary.
MSTM/CW10/08 4-11
– Mount filter wheel. Turn the dark filter in front of the multiplier.
– Unscrew the two T20 Torx screws that hold the flange of the filter wheel motor and screw it onto the new module in
such a way that the gear wheel of the filter wheel motor catches at a right angle in the teeth of the filter wheel without
play. Now tighten the two screws again.
– In the last step the ground connection is transferred.
– Insert the new multiplier module in such a way that the angled rubber seal is subjected to only light pressure.
Make sure that no cables of the light barrier or the motor are caught beneath the multiplier module and that the
module sits correctly on the base plate with the two adjustment pins.
– Tighten the two T20 Torx screws that hold the multiplier module.
– Connect the two X7 and X8 plugs of the ribbon cable for the multiplier to the PCB Control and position in a bow
shape as shown in the picture below.
Bend multiplier cable into a bow shape!
Black heat shrink Black heat shrink

tube tube
Fig. 11
Fastening screws Fastening screws
of the multiplier module of the multiplier module
channel 2 channel 1
– Position the black heat shrink tube in such a way that it functions as an upper and lower edge protection (see picture
11).
– Also connect plug X 207 for the PCB Amplifier to the PCB Control. Also position the cable in a bow shape.
– Position the ground plate correctly and tighten the 4 x T20 Torx screws.
– Adjustment of the realplex module is then carried out with the help of the commissioning software.
See Ch. 3 "Commissioning Software".
4-12 MSTM/CW10/08
Replace light barrier(s) or motor for filter wheel

Slotted screwdriver M4
Commissioning software (CSW)
– Put on gloves.
Conditions:
– Turn module upside down and loosen the 4x T20 Torx screws from the ground plate.
– Lift the ground plate and remove plugs X48 and X2 (channel 1) / X 49 and X4 (channel 2) for the relevant light barrier
or motor for filter wheel from the PCB Control.
1 6 SCH21 SCH22 1 6
X7 X3 X8
1
X19 X20 SL
C C
C C
4
4
8 1
8 1
2
UP
C334 C335
2
9 6
3
1 1 C326
SCH20 OK20 R213 5 1
C220
R220
R35
IC33 R221 TLP113

X207 C28 IC34
C327
2 10 IC28
R36
82C250
R290
PCA
R222
C201
DR1 TLP113 MAX202

SPR20
IC6 1
R24
C221
C200
OK21
R223
IC29
74HCT125
C222
C202 C203
Q1 C32
C39
C224
R29
C29 C332
C227 C22 R207
K6T8016C3M
BS616LV4015
C26
C33
R208
C330
DR31
SPR32
C38
R204 LM2594
TC7662B
*
J200
D31
SPR31
IC26 IC200 R205 IC35
MP224
C329
D30
R300 HOLDER
R206 MP222
C206
C23
R301 C35
direction
SCH1
C37 C331 DR30

Switch
C27
C167_CS
C25
C34
IC20 MP218 R298

M29F160BT
C36
R3
IC25
R299 C305
C314
R225
C302
R25
74HCT02
R224 FE30 IC19 C315

FE33
C127
FE29
FE14
FE13
FE16
C304
FE7
C123
FE12
FE18
FE10
C313
FE15
C306
FE9
C228
IC31
C229 C223 C24

FE8
FE5
FE32
C311
C337
FE17
IC32
C307
C309
R23
C316
R32
R296 SPR34
74AC04
FE6
14 26
LEDMATRIX IC21
C318
FE19
R34
F1
C308
R295
FE4 LM78l05
FE31
R22
R31
IC201 X1
C110
C119
C117
C112
C104
C102
C103
C121
C120
C118
C107
C106
*
1 13 C340
C30
FE20 C338 C301

R303 C225
C317
FE1
FE2
FE3
FE11
R33
C105
C125
C126
IC30
C303
C108
C109
C116
C111
C113
C115
C114
C124
R27
74HC14
R28 IC24 C310

C204
1 X10 22 C312
R30 C31
IC22 1 4 FE28
IC23
X4
FE25
BAT1
X4
Q2 DS1307
24C65 C226
SCH2
FE26
FE27
C21
R203
C209
X49
C208
R202
1 5
HEATINGFOIL X49
MAX808
D20 FE22
R214
R297 IC27 20 10 1
1 4
SPR33 C336
X9 X2 FE24
R21
R20
Label FE23
1 1 FE21 R200
C333
GND
GND
GND
X2
SHIELD
1 5
X48
SDA_2
SCL_2
LID_+
R322
LID_-
SHIELD
R226
X13
C207
R201
IC202
X11
T1 R302 R323
ROLLUP CABLE 1
X51
6
FAN TEMP. X48
SENSOR
C210
R294
R292
R293
R291
C211
MMSTM/CW10/08 4-13
Temperature sensor
7 x slotted screw
Cover plate
Filter wheel
motor
Light barrier
channel 2
Cable sleeve
Filter wheel motor
Multiplier spring
Light barrier Multiplier

channel 1
– Unscrew relevant screws.

– Fix the ground plate in place with at least two screws.
– Cut open three tie wraps.
– Unscrew the seven slotted screws of the cover plate.
– Dismantle multiplier spring.
The multiplier spring is no longer necessary when a new multiplier module is to be used.
However, the T20 Torx screw for the spring is once again screwed into the threads in order to prevent the incidence
of stray light.
– Lift cover plate.

– Release the relevant cable sleeve for the light barrier / filter rotor motor, pull in the cable with plugs and lift out the
pinned assembly together with the multiplier.
4-14 MSTM/CW10/08
Disassembly of light barrier
Disassembly of light barrier

– The light barrier is fixed in place with two T10 Torx screws.
Disassembly of filter rotor
To replace the motor for the filter wheel the filter rotor first has to be removed.
– Release the M2 socket-head screw fixing the filter rotor shaft in place.
– Turn the filter rotor to a position where no filter coding vane is in the light barrier.
– Remove the filter rotor.
– The realplex module is then adjusted using the commissioning software. See section 3 "Commissioning software".
Upgrading of a Mastercycler ep to a body cycler for realplex module
Note: Upgrading is only possible with Thermoblocks 96 Al or 96 Ag.

Aux. aids: Phillips screw driver M3
Allen key M4, M1.5
Torx screwdriver TX 10 and TX 20
Mounting set cycler 6302 862.000
Adjusting plate with special Torx screwdriver 0024 217.108
Description of cleaning of Thermoblock, see operating manual.

Description of testing of Thermoblock using background calibration, see operating manual.
Service parts as required: Repair kit p. supply unit 5341 896.004
PCB Power unit 5341 852.007
PCB Launch 5341 853.003
Thermoblock 96 Ag 5341 859.001
or Thermoblock 96 Al 5341 857.009
Pretesting of Mastercycler ep
Before conversion of the Mastercycler ep to the realplex system the service function "Index Units" is used to determine
the hardware status of the cycler.
The PCB Power unit (Power) must have the index –09 or greater to function with the realplex system.
If the index is smaller than –09, the PCB must be replaced.
If the Thermoblock is not clean after cleaning and testing using Commissioning software , the PCB Launch has an index
smaller than –08 and the Thermoblock an index smaller than –10, the PCB Launch and Thermoblock must be replaced.
If the PCB Launch has an index –08 or greater and the Thermoblock an index –10 or greater, the Thermoblock is only
replaced as required.
It is not possible to interrogate the hardware status of the switching power supply unit using the service function "Index
Units".
If the index of the Power supply unit is smaller than –04 siehe sticker, the Power supply unit has to be modified. The
repair kit 5341 896.004 is used for this purpose.
cont'd on next page Î
MSTM/CW10/08 4-15
Upgrading of Mastercycler ep to a Mastercycler ep realplex system
Remove the plug for the panel on the rear of the device and put the panel to one side.
The panel is no longer required.
Disassembly of Heated lid
– Put the heated lid into the rear position and move up.
– Remove the silver cover plate in the Heated lid.
– Remove the strain relief.
– Remove the Rollflex cable from the PCB Heated lid.
– Refit the strain relief and the cover plate.
– Dismantle the rear panel of the device.
– Move down the Heated lid and pull out from the back of the device, making sure that the sprung guide pins do not
get lost.
The heated lid is no longer required.
Disassembly of stop eccenter
– Unscrew the Torx screw to remove the stop.

– Attach a blank label from the mounting set over the taphole of the stop.
Disassembly of X-drive (if present)
– Remove board rack.

– Remove PCB X-drive.
– Remove PCB Position.
– Release 1.5 mm socket-head in cog wheel.
– Remove cog wheel.
– Unscrew motor fastening screws.
– Remove X–drive.
– Attach label "for M Option" to the hole through which the motor axle was inserted.
4-16 MSTM/CW10/08
Installation of mounting set
The mounting set 6302 862.000 consists of: 2x centering pin

2x countersunk screw M4x6
1x guiding rail
1x blank label
1x label marked "for M Option"
1x strain relief for Rollflex cable
4xstopper
Countersunk screws
Guiding rail
Centering pin
Rollflex cable
Label "for M Option" Stop eccenter
– Mount the guiding rail with the two countersunk screws.
– Remove the two stoppers from the Thermoblock and screw down the two centering pins securely.
MSTM/CW10/08 4-17
Mount realplex module
Stopper
Lever
Slide bar
Pin
Lock Slide bar
– Hook the locks of the module into the two pins of the slide bar.
– Move the lever on the module into the rear unlocked position.
– Insert the slide bars of the realplex module into the guiding rail of the Mastercycler at the rear and slide to the front
stop.
– Mount the rear panel of the cycler.
– Slide the realplex module to the stop at the rear.
– Then unlock the module and open.
Attention: Make sure that the stand snaps into place properly.
– Next the Rollflex cable is inserted into the blue plug of the module. Ensure correct insertion.
– Mount strain relief.
– Place adjusting plate in Thermoblock.
– Unlock the stand, move down the module and slide back into the front position.
4-18 MSTM/CW10/08
Alignment of realplex module with Thermoblock
Stopper
Stopper
– Remove the four stoppers in the top of the realplex module.

– Use the special Torx screwdriver to slightly loosen the four screws of the guide sockets. When doing so, the lever
has to be held in different positions to access the four screws.
– Open and close the lever several times so that the guide sockets properly engage in the centering pins and the
heating foil frame in the adjusting plate.
– Move lever in forward position.
– Then tighten the screws in forward position.
– Move lever in backward position and tighten the screws in backward position securely.
The mechanical retrofitting is thereby complete.
Adjustment of the realplex module is then carried out with the help of the commissioning software (CSW). See Ch. 3
"Commissioning Software".
MSTM/CW10/08 4-19
Mounting of realplex module on a body cycler
Aux. aids: Torx screwdriver T20

Instruction manual 6302 900.378
Lever
Slide bar
Lock and pin
Fig. 1
– Dismantle the rear panel of the Mastercycler.

– Hook the locks of the realplex module into the two pins of the slide bar.
– Move the lever on the module into the rear unlocked position.
– Insert the slide bars of the realplex module into the guiding rail of the Mastercycler at the rear and slide to the front
stop.
– Mount the rear panel of the cycler.
– Slide the realplex module to the stop at the rear.
– Then unlock the module and open. Make sure that the stand snaps into place properly.
– Next the Rollflex cable is inserted into the blue plug of the module. Ensure correct insertion.
– Mount strain relief.
Locks
Locks
Stand
– Unlock the stand, move down the module and slide back into the front position.
Follow installation in the instruction manual.
4-20 MSTM/CW10/08
PMT2
900 nm
Signal (CL)
Ground (BK)
PMT1
650 nm
Signal (CL)
Ground (BK)
Mastercycler® ep 6302 – 7 Spare parts list
7 Ersatzteilliste
1-3
4-7
3-6
3-3
4-10
2-10
2-8 2-5
2-12
2-3
3-2
2-11
2-6
4-3
2-6
2-4
2-11
4-4
4-8
3-1
Fig. 1
3-4 4-4 4-2
MSTM/2307 7-1
Note: Spare parts for the Mastercycler ep, German can be found in the service manual 5341 900.028
7 Ersatzteilliste
and/or for the Mastercycler ep, English in the service manual 5341 900.036
Fig. Item Designation Part number
1 1 Housing
1-1 Housing realplex 2 6302 876.000
1-2 Housing realplex 4 6302 877.007
1 1-3 Stopper, set 6302 890.003
2 Light path
System 1 (PE)
1 2-3 Multiplier 900nm with power supply 6302 857.006
1
1 2-4 Multiplier 650nm with power supply * 6302 858.002
2-6 Filter 520nm, with diffuser 6302 101.203
Motor for filter wheel 6302 855.003
Light barrier filter recognition 6302 856.000
1 2-5 Beam splitter 6302 866.005
1 2-6 Filter wheel 6302 867.001
1 2-8 Frame with heating foil and Isolation plate 6302 866.501
Pads for realplex (set of 10) 6302 896.001
1 2-9 Sleeve 6302 884.003
1 2-11 Light conductor module (incl. socket for ref. channel and tool) 6302 874.008
Tool for light conductor 0024 217.205
3 Electric/ Electronic
1 3-1 PCB control universal 6302 860.503
1 3-2 PCB Amplifier 6302 859.009
1 3-3 PCB LED Matrix 6302 851.008
1 3-4 LED status + microswitch 6302 853.000
3-5 Fuses 6302 860.007
1 3-6 Fan ODM 6302 852.004
4 Mechanical parts
4-1 Cycler assembly kit 6302 862.000
1 4-2 Set of slider with stand 6302 881.004
1 4-3 Set of guiding plates 6302 882.000
1 4-4 Lock, set 6302 879.000
4-5 Spring small, set 6302 880.008
*1 This multiplier requires a new housing.
7-2 MSTM/2307
7 Ersatzteilliste
Fig. Item Designation Part number
4-6 Spring big, set 6302 887.002

1 4-7 Lever 6302 872.005
1 4-8 Hydraulics piston, cpl. 6302 889.005
4-9 Strain relief (rollflex cable) 6302 892.006
1 4-10 Reference channel 6302 899.000
5 Auxiliary aids
5-1 Test box realplex 6302 875.004
5-2 Certification of "Test box realplex" 6302 875.306
5-3 Adjusting plate 0024 217.108
Adjustment filter set 6302 863.502
5-6 USB-CAN adapter 5349 851.000
5-7 PC-CAN bus cable 5349 852.006
5-8 Spacer for thermal validation 6302 891.000
5-9 Packaging 6302 853.507
5-10 Temperature indicator 6302 893.002
5-11 Gloves (1 pair) 0019 031.381
5-12 Torx screwdriver T10 0024 217.809
5-13 Torx screwdriver T20 0024 217.909
MSTM/2307 7-3

7 Ersatzteilliste
Recommended spare parts – initial equipment
To: Eppendorf AG From:
Fax no: +49 40-5 38 01-758 Fax no.:
Name:
Tel.:
Order no.:
Quan- Fig. Item Designation New part

tity
2 Light path
2-1 Motor for filter wheel 6302 855.003
2-2 Light barrier filter recognition 6302 856.000
1 2-7 Frame with heating foil 6302 861.003
1 2-9 Sleeve 6302 884.003
3 Electric/ Electronic
1 3-1 PCB control universal 6302 860.503
1 3-2 PCB Amplifier 6302 859.009
1 3-3 PCB LED Matrix 6302 851.008
1 3-4 LED status + microswitch 6302 853.000
1 3-6 Fan ODM 6302 852.004
4 Mechanical parts
1 4-2 Set of slider with stand 6302 881.004
1 4-8 Hydraulics piston, cpl. 6302 889.005
4-9 Strain relief (rollflex cable) 6302 892.006
5 Auxiliary aids
5-1 Test box realplex 6302 875.004
5-3 Adjusting plate 0024 217.108
5-4 Pale filter, 2 pcs. 6302 898.004
5-10 Gloves (1 pair) 0019 031.381
7-4 MSTM/2307
Mastercycler® ep 6302 – 8 Technical Data
Technical data Mastercycler ep realplex
Excitation source 96 LEDs (470 nm)

Emission filters 520 ±10 nm / 550 ±10 nm (realplex2)
520 ±10 nm / 550 ±10 nm / 580 ±5 nm / 605 ±15 nm (realplex4)
8 Technical Data
Photomultiplier 1 CPM (realplex2)
2 CPMs (realplex4)
Sample capacity 96 x 0.2 ml PCR tubes or one 96 PCR plate
(unskirted, semi-skirted, skirted - as per SBS standard)
Temperature control range of block 4 °C to 99 °C
Range of gradient 1 °C to 20 °C (thermo module Mastercycler ep)
1 °C to 24 °C (thermo module Mastercycler ep S)
Temperature control range of gradient 30 °C to 99 °C
Temperature of lid 105 °C
Block homogeneity 35 °C ± 0.3 °C
90 °C ± 0.4 °C
Control accuracy ± 0.2 °C
Heating rate* approx. 4 °C/s (thermo module Mastercycler ep)
approx. 6 °C/s (thermo module Mastercycler ep S)
Cooling rate* approx. 3 °C/s (thermo module Mastercycler ep)
approx. 4.5 °C/s (thermo module Mastercycler ep S)
Dimensions (W x D x H) 26 cm x 41 cm x 39.6 cm
Total weight 24 kg
Weight of thermo module 17 kg
Weight of detection module 7 kg
Mains power connection 100 V - 130 V, 50 - 60 Hz,
200 V - 240 V, 50 - 60 Hz
(Mains power deviation not greater than ± 10% of nominal mains power)
Power consumption 800 W
Over voltage category II (IEC 610 10-1)
Contamination level 2
Protection class 1
IP protection 20
MSTM/2205 8-1
Mastercycler® ep 6302 – 8 Technical Data
Ambient conditions (only indoors) 15 °C to 35 °C, 70% rel. humidity, to 2000 m sl

Storage conditions -20 °C to 50 °C, 85% rel. humidity
Transport conditions -20 °C to 50 °C, 85% rel. humidity
* measured at block
8 Technical data
Technical specifications subject to change!
Mains power connection supply and power consumption of Mastercycler ep realplex module via connected
thermo module.
The device has been awarded the CE mark
8-2 MSTM/1705
Mastercycler® ep 6302 – 9 Care and maintenance
Device
The outer surfaces of the Mastercycler ep realplex system should be cleaned regularly with a cleaning agent.
If hazardous material contaminates the device, the owner is responsible for appropriate cleaning and decontamination.
9 Maintenance
Returning devices
When returning the Mastercycler ep realplex system, ensure that the system is fully decontaminated and does not pre-
sent any health risk to our service staff.
For further information and a decontamination certificate, please visit www.eppendorf.com. Do also consult your labora-
tory safety officer about a suitable decontamination method.
Please fill in the decontamination form and enclose it with the device if it is to be returned to Eppendorf.
KS/1209 9-1
Specification for on-site disinfection of the Mastercycler
The disinfectant must be free of fluorescing substances.

Fluorescing substances falsify measurement result of the Mastercycler ep realplex.
9 PMaintenance
Eppendorf recommends Bacillol AF as Disinfectant.

Source: BODE CHEMIE Hamburg / Germany
Homepage BODE CHEMIE: http://www.bode-chemie.com
E-Mail address: info@bode-chemie.de
Mastercycler modules
The Mastercycler ep realplex system comprises two modules.

Thermomodul
The lower module is the thermomodule (Mastercycler).
The thermoblock is located inside this module.
realplex module
The upper module is the realplex module.
The frame with heating foil and the highly sensitive optics, etc., are located in this module.
Mastercycler ep realplex system
The Mastercycler ep realplex system may only be shipped in the original Eppendorf packaging.
Order number
Packaging, Realplex module: 6302 853.507
Disinfection procedure
Disinfection of the Realplex module

The Realplex module is disinfected on the surface only.
If the spray disinfection method is used, make sure the spray mist does not spread out beneath the realplex module, and
thence into the heating frame and highly sensitive optics. This will damage the device and so generate high subsequent
costs.
Disinfection of the thermomodule
When disinfecting the surface of the thermomodule, make sure no liquid enters the blue plug on the
Rollflex cable.
1 If the thermoblock is covered by a PCR plate 96, the plate must be removed.
2 Now the visible surface can be disinfected.
3 Replace the PCR plate 96 in the thermoblock after the disinfection.
Make an entry on the Disinfection Certificate to the effect that the heating foil was not disinfected.
9-2 KS/1209
Group 1 Group 2 Group 3
Classic Cycler Mastercycler ep and pro Mastercycler ep realplex
Maintenance of PCR Systems 5331 5332 5333 5334 5341 5344 5345 6300 6302
6321 6324 6325
Nr. Completion recommended every months: 12 12 12 12 12 12 12 12 12
M 01 Cleaning the device according to instruction manual x x x x x x x x x
M 02 Analyze device data and write down if necessary x x x x x x x x x
M 03 Keyboard x x x x x x x
M 04 Visual inspection of display x x x x x x x
M 05 Stability x x x x x x x x x
M 06 Cleaning the internal assemblies x x x x x x x x x
M 07 Housing x x x x x x x x x
M 08 Printed circuit board/current supply x x x x x x x x x
M 09 Heated lid x x x x x x x
M 10 Heated lid for Mastercycler pro x x x
M 11 Thermoblock x x x x x x x x x
M 12 Motor fan/device fan x x x x x x x x x
M 13 realplex module x x
M 14 Software update x x x x x x x x x
M 15 Conduct system test x x x x x x x x x
Average time expenditure 2.5 to 3 hours 2 to 2.5 hours 3 to 3.5 hours
The indicated times are only valid for devices that are not more than four years old.
For older devices, the time expenditure can be higher.
Spare parts which may be required are not included in the expenditure.
For the models 5331/32/33/34, a thermal validation is calculated with software version 2.1 or higher.
For the models 5331/33/34, a software update is only calculated with empty internal memory and for systems without EPROM exchange.
KS/1209
Operational Qualification (OQ) Classic Cycler Mastercycler ep and pro Mastercycler ep realplex
including electrical test 5331 5332 5333 5334 5341 5344 5345 6300 6302
6321 6324 6325
Completion recommended every months: 12 12 12 12 12 12 12 12 12
Operation Qualification (OQ) including electrical test x x x x x x x x x
Average time expenditure 2 hours 2 hours 2 hours

Extra Maintenance Service Classic Cycler Mastercycler ep and pro Mastercycler ep realplex
of PCR Systems 5331 5332 5333 5334 5341 5344 5345 6300 6302
6321 6324 6325
ME 01 Software upgrade x x x x x x x x x
Average time expenditure 15 minutes 10 minutes 30 minutes

ME 02 Electrical test x x x x x x x x x

ME 03 Thermal validation x x x x x x x x x

ME 04 Background Calibration x x
Average time expenditure 15 minutes

ME 05 Color Calibration (incl. Background Calibration) x x
Average time expenditure 60 minutes
The indicated times are only valid for devices that are not more than four years old.
For older devices, the time expenditure can be higher.
Spare parts which may be required are not included in the expenditure.
For the models 5331/32/33/34, a thermal validation is calculated with software version 2.1 or higher.
For the models 5331/33/34, a software update is only calculated with empty internal memory and for systems without EPROM exchange.
KS/1209
Description: Maintenance of PCR Systems
M 01 Cleaning the device according to instruction manual

Clean PCR systems as described in the respective operating manual and supplement sheets.
M 02 Analyze device data and write down/delete if necessary

(Note: The individual parameters vary, or are not available, based on the PCR system.)
9 Maintenance
Analyze the following parameters with the service program:
– Error statistics
– Number of cycle counts
– Number of pulse counts
– Number of program counts
M 03 Keyboard
Check the keyboard by pressing all keys.
M 04 Visual inspection of display

Check the display with the corresponding service program ( if available).
When doing so, pay special attention to the following points:
– Defective pixels
– Background lighting
M 05 Stability
Check the stability of the device feet.
They must be replaced if fissures or damages are present.
M 06 Cleaning the internal assemblies

Disassemble/assemble the PCR systems as described in the respective service manual and
supplement sheets.
M 07 Housing
Remove dust and soiling with a cleaning brush, or, if necessary, compressed air.
Pay attention to damages that compromise safety.
With existing fissures, there is the possibility of unintentional infiltration of liquids. If this occurs, the
housing must be replaced.
M 08 Printed circuit board / current supply:

M 09 Heated lid
Clean dust and soiling with a cleaning brush, or, if necessary, compressed air.
Clean the slide rails for the heated lid with a lint-free cloth.
M 10 Heated lid for MC pro

Inspect the flexible pressure element of the vapo protect heated lid for damages.
M 11 Thermoblock
When cleaning a realplex thermoblock, the cleaning procedure described in the instruction manual must
be observed.
M 12 Motor/device fan:
Pay attention to operating noise.
With the service program, select various voltages for testing. ( if available )
M 13 realplex module
Clean the frame with heating foil, insulating plate, and lower lens array as described in the respective
service manual.
KS/1209 9-5
M 14 Software update
Install the latest software in order to utilize the product improvements offered by new functions.
The latest software is available for download at www.eppendorf-support.com.
M 15 Conduct system test

After the maintenance, we recommend conducting an OQ, including the test for electrical safety.
9 PMaintenance
Description: Extra Maintenance Services of PCR systems
ME 01 Software upgrade
Install the latest software (available for a fee) in order to utilize the product improvements offered by new
functions.
The latest software is available for download at www.eppendorf-support.com.
ME 02 Electrical test
See SOP for PCR systems.
ME 03 Thermal validation
For the temperature test, use the Eppendorf temperature validation set 0055 000.298.
ME 04 Background Calibration
See Operating manual Mastercycler ep realplex.
ME 05 Color Calibration (incl. Background Calibration)

See Operating manual Mastercycler ep realplex.
9-6 KS/1209
Electrical safety test
Effective standard
IEC 61010-1 (complies with EN 61010-1)
Measuring device
9 Maintenance
The specifications of the above-mentioned standard must be able to be measured using the measuring device.
Consult the manufacturer of your measuring device.
Only a properly instructed and duly qualified electrician may carry out electrical safety tests.
Notes concerning the measuring procedure

Measurement is carried out in accordance with EN 61010-1.
Measurement pursuant to any other, country-specific standard (e.g. VDE 701/702) will be allowed only if this standard
specifies the same measuring procedures and limiting values and is subordinated to EN 61010-1.
If the measurement is carried out in accordance with any other standard, attention must be paid to the fact that the
measuring arrangement meets the requirements of EN 61010-1.
In the US, the PE conductor current is measured in accordance with NFPA 99.
Handling of the different measuring devices may vary.
Consult the operating manual of the measuring device for information about the measuring procedure.
Measurement is carried out on a grounded, metallic point that is not insulated and can be touched from the outside
(e.g. housing screws).
Never use an optionally provided interface for measurement. You might damage the electronic system of the device.
KS/1209 9-7

9 PMaintenance
9-8 KS/1209
SM Mastercycler® ep realplex 6302 6302 900.521-07
eppendorf ist eine eingetragene Marke
Eppendorf AG · 22331 Hamburg · Germany · Phone: +49 40 538 01 0 · Fax: +49 40 538 01 310
E-Mail: eppendorf@eppendorf.com · Internet: www.eppendorf.com

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Service Manual · English

Valid for devices with the application software up to version 1.5

Service Manual Mastercycler® ep realplex 6302 · English · 6302 900.521-07

PACT information sheets 2

Error lists / Troubleshooting 3

(blank) Alignment / Adjustment 5

Spare parts list 7

Care and maintenance 9

Mastercycler® ep 6302 – Contents

Safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

3 Error lists / Troubleshooting

User error list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

Disassembly of upper part of housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

Mastercycler® ep 6302 – Contents

Disassembly of filter rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15

Upgrading of a Mastercycler ep to a body cycler for realplex module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15

General circuit diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6302_900.491-01

7 Spare parts list

Spare parts list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1

Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1

9 Care and maintenance

Mastercycler® ep 6302 – 1 Safety

Explanation of warning and hazard symbols in this Service Manual

Refers to particularly useful information and tips.

Mastercycler® ep 6302 – 1 Safety

Danger! Electric shock.

Risk when handling toxic or radioactively-marked liquids or pathogenic germs.

Warning! Risk of injury from sharp-edged components.

Warning! Burns from hot thermoblock and heated lid.

Warning! Risk of burns.

Warning! Injury from trapping or crushing.

Caution! Damage to electronic components and the lens from condensation.

Caution! Material damage from incorrect connections.

Warning! Damage due to incorrect power supply.

Mastercycler® ep 6302 – 1 Safety

Caution! Damage due to overheating.

Caution! Damage to the device from incorrect operation.

Caution! Damage to the thermoblock from incorrect operation.

Warning! Risk to health if used incorrectly.

Warning! Poor safety due to incorrect accessories.

Caution! Damage to the display from mechanical pressure.

Caution! Ensure that service manuals are up to date.

Danger! Electric shock as a result of penetration of liquid.

Mastercycler® ep 6302 – 1 Safety

Caution! Service and maintenance by authorized personnel only.

Mastercycler® ep 6302 – 2 PACT information sheets

2 PACT information sheets

Mastercycler ep realplex System

Mastercycler ep realplex system

2008-06-02 /KS / UK Mastercycler ep realplex 8 / 01 1 (1)

Die neue LP-Netzteil ( 6321 856.509 ) ersetzt die bisherigen

Technical Information_6321_PCB Power supply unit (6321 856.509).doc

Im Mastercycler ep 5341 / 5344 / 5345 und im Mastercycler realplex 6300 / 6302

PCB SMPS 6321 895.008

Technical Information_6321_ PCB SMPS ( 6321 895.008 ). doc

Mastercycler® ep 6302 – 2 PACT information sheets

Mastercycler® ep 6302 – 3 Error lists / Troubleshooting

User error list

Error code Internal error Cause Remedy