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Class 12worksheet On Biotechnology

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26 views2 pages

Class 12worksheet On Biotechnology

Uploaded by

spandeydis
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Class 12

Biotechnology : Principle and Process

1-Mark Questions
1. What is biotechnology?
2. Name one application of biotechnology in medicine.
3. What is the function of restriction enzymes in genetic engineering?
4. Define a plasmid.
5. What does PCR stand for in biotechnology?
6. Name one type of vector used in genetic engineering.
7. What is a transgenic organism?
8. Which enzyme is used to join DNA fragments in recombinant DNA technology?
9. What is the primary purpose of gel electrophoresis?
10. What is gene cloning?
11. Name one application of biotechnology in agriculture.
12. What is the role of ligase in recombinant DNA technology?
13. Which technique is used to amplify specific DNA sequences?
14. What is the purpose of the CRISPR-Cas9 system in biotechnology?
15. What type of molecule is DNA?

2-Mark Questions
1. Define biotechnology and mention two areas where it is applied.
2. What is genetic engineering? Provide one example of its application.
3. Explain the role of restriction enzymes in genetic engineering.
4. What is a plasmid, and why is it important in recombinant DNA technology?
5. Define the term ‘transgenic organism’ and give an example.
6. What is the purpose of the polymerase chain reaction (PCR) in biotechnology?
7. Briefly describe what is meant by 'gene cloning.'
8. What is a recombinant DNA molecule?

3-Mark Questions
1. Describe the basic steps involved in gene cloning using plasmids.
2. Explain the principle of gel electrophoresis and its role in DNA analysis.
3. What is the significance of the CRISPR-Cas9 system in biotechnology?
4. Discuss the process and applications of the polymerase chain reaction (PCR).
5. Explain the role of ligase enzyme in recombinant DNA technology.
6. Describe the process of creating a genetically modified organism (GMO) from a bacterial
plasmid.
7. What is the role of a vector in genetic engineering? Provide examples of common vectors
used.
8. Discuss the principle and applications of DNA fingerprinting.

5-Mark Questions
1. Explain the process of recombinant DNA technology, including the steps of gene isolation,
insertion, transformation, and screening.
2. Describe the principle and methodology of polymerase chain reaction (PCR). How is PCR
used to amplify DNA samples?
3. Discuss the process of producing a transgenic plant. Include steps such as gene insertion,
selection, and regeneration.
4. Explain the role of biotechnology in medicine, providing examples such as the production
of insulin or vaccines.
5. Describe the process of gene therapy, including the methods of delivering therapeutic
genes to cells. What are the potential benefits and challenges of gene therapy?
6. Explain the technique of gel electrophoresis in detail. How does it separate DNA
fragments, and what information can be derived from the electrophoresis results?
7. Discuss the use of biotechnology in agriculture, focusing on genetically modified crops and
their benefits and concerns.
8. Describe the steps involved in the production of a genetically modified bacterium for
industrial use. Include the roles of cloning vectors and selection markers.

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