Gonoyostomum sp

Cell density(cells/ml)
R1
Day date upper lower total cell density
0 80 93 173 865000
1 165 115 280 1400000
2 65 125 190 950000
3 150 135 285 1425000
4 175 240 415 2075000
5 260 456 716 3580000
6 310 600 910 4550000
7 750 1100 1850 9250000
8 965 1065 2030 10150000
9 620 425 1045 5225000
10 455 290 745 3725000

Days cell SE
0 0.06166666667 0.0209609425
1 0.09416666667 0.0310353741 Growt
2 0.08 0.0202072594 1.4
3 0.1225 0.01040833
1.2
4 0.22166666667 0.0193828389

CD (Cells/ml*107)
5 0.51083333333 0.0766454681 1
6 0.70133333333 0.1233951422 0.8
7 1.16833333333 0.1277557348
8 0.9425 0.0814068998 0.6
9 0.527 0.0357223086 0.4
10 0.371 0.0215193711
0.2
0
0 1
Gonoyostomum sp

R2 R3
cells/ml *107 Upper lower total cell density cells/ml 107 Upper lower
0.0865 24 16 40 200000 0.02 68 89
0.14 45 25 70 350000 0.035 90 125
0.095 30 50 80 400000 0.04 80 130
0.1425 100 135 235 1175000 0.1175 110 105
0.2075 140 255 395 1975000 0.1975 145 375
0.358 364 790 1154 5770000 0.577 605 590
0.455 585 1090 1675 8375000 0.8375 810 813
0.925 1060 1385 2445 12225000 1.2225 1285 1430
1.015 640 1425 2065 10325000 1.0325 790 770
0.5225 190 745 935 4675000 0.4675 528 654
0.3725 156 510 666 3330000 0.333 396 419

Growth Curve of Gonyostomum sp in Outdoor

1.4
1.2
CD (Cells/ml*107)

1
0.8
0.6
0.4
0.2 1.4

0 1.2
0 1 2 3 4 5 6 7 8 9 10
Culture Days 1

0.8

0.6

0.4

0.2

0
0 1
 total cell density cells/ml 107 Average SD SE
157 785000 0.0785 0.06166666667 0.0363054174103 0.0209609425149
215 1075000 0.1075 0.09416666667 0.0537548447429 0.0310353740826
210 1050000 0.105 0.08 0.035 0.0202072594216
215 1075000 0.1075 0.1225 0.0180277563773 0.0104083299973
520 2600000 0.26 0.22166666667 0.0335720617975 0.019382838916
1195 5975000 0.5975 0.51083333333 0.132753844891 0.0766454680838
1623 8115000 0.8115 0.70133333333 0.2137266556453 0.1233951421698
2715 13575000 1.3575 1.16833333333 0.2212794236556 0.1277557348137
1560 7800000 0.78 0.9425 0.141000886522 0.0814068997895
1182 5910000 0.591 0.527 0.0618728534981 0.0357223086227
815 4075000 0.4075 0.371 0.0372726441241 0.0215193711184

cell
1.4

1.2

0.8

0.6

0.4

0.2

0
0 1 2 3 4 5 6 7 8 9 10
 Gonoyostomum

PH 7
TIME Initial ab R1 R2 R3 HE(R1) HE(R2) HE(R3) AVERAGE
10 0.632 0.512 0.51 0.515 18.9873418 19.3037975 18.5126582 18.9345992
20 0.632 0.513 0.511 0.511 18.8291139 19.1455696 19.1455696 19.0400844
30 0.632 0.508 0.509 0.508 19.6202532 19.4620253 19.6202532 19.5675105
40 0.632 0.505 0.505 0.507 20.0949367 20.0949367 19.778481 19.9894515
50 0.632 0.503 0.504 0.503 20.4113924 20.2531646 20.4113924 20.3586498
60 0.632 0.503 0.505 0.502 20.4113924 20.0949367 20.5696203 20.3586498

time(min) 7 8 9
10 18.9345992 19.9367089 18.2489451
20 19.0400844 22.5738397 21.2552743
30 19.5675105 23.1540084 21.3080169
40 19.9894515 24.314346 23.2067511
50 20.3586498 25.2637131 24.5780591
60 20.3586498 26.1603376 25.5801688
 PH 8
SD SE Initial ab R1 R2 R3 HE(R1) HE(R2) HE(R3)
0.39819802 0.22989973 0.632 0.503 0.507 0.508 20.4113924 19.778481 19.6202532
0.18270578 0.10548523 0.632 0.488 0.491 0.489 22.7848101 22.3101266 22.6265823
0.09135289 0.05274262 0.632 0.487 0.484 0.486 22.943038 23.4177215 23.1012658
0.18270578 0.10548523 0.632 0.476 0.479 0.48 24.6835443 24.2088608 24.0506329
0.09135289 0.05274262 0.632 0.471 0.474 0.472 25.4746835 25 25.3164557
0.24169703 0.13954385 0.632 0.469 0.466 0.465 25.7911392 26.2658228 26.4240506

Chart Title
10 11 120
22.8902954 60.7067511
100
26.7932489 68.8818565
29.9578059 84.7046414 80
32.964135 86.2869198
35.8122363 97.0464135 60
40.1898734 97.7848101
40

20

0
1 2 3 4 5 6 7

Column F Column G Column H Column I Column J Column K

 PH 9
AVERAGE SD SE Initial ab R1 R2 R3 HE(R1) HE(R2)
19.9367089 0.41863154 0.24169703 0.632 0.517 0.518 0.515 18.1962025 18.0379747
22.5738397 0.24169703 0.13954385 0.632 0.497 0.499 0.497 21.3607595 21.0443038
23.1540084 0.24169703 0.13954385 0.632 0.499 0.496 0.497 21.0443038 21.5189873
24.314346 0.32937753 0.19016621 0.632 0.486 0.487 0.483 23.1012658 22.943038
25.2637131 0.24169703 0.13954385 0.632 0.474 0.477 0.479 25 24.5253165
26.1603376 0.32937753 0.19016621 0.632 0.47 0.469 0.472 25.6329114 25.7911392

J Column K
9 PH10
HE(R3) AVERAGE SD SE Initial ab R1 R2 R3 HE(R1)
18.5126582 18.2489451 0.24169703 0.13954385 0.632 0.487 0.489 0.486 22.943038
21.3607595 21.2552743 0.18270578 0.10548523 0.632 0.462 0.464 0.462 26.8987342
21.3607595 21.3080169 0.24169703 0.13954385 0.632 0.444 0.443 0.441 29.7468354
23.5759494 23.2067511 0.32937753 0.19016621 0.632 0.423 0.425 0.423 33.0696203
24.2088608 24.5780591 0.39819802 0.22989973 0.632 0.406 0.404 0.407 35.7594937
25.3164557 25.5801688 0.24169703 0.13954385 0.632 0.379 0.377 0.378 40.0316456
PH10 PH11
HE(R2) HE(R3) AVERAGE SD SE Initial ab R1 R2 R3
22.6265823 23.1012658 22.8902954 0.24169703 0.13954385 0.632 0.248 0.251 0.246
26.5822785 26.8987342 26.7932489 0.18270578 0.10548523 0.632 0.194 0.199 0.197
29.9050633 30.221519 29.9578059 0.24169703 0.13954385 0.632 0.099 0.096 0.095
32.7531646 33.0696203 32.964135 0.18270578 0.10548523 0.632 0.088 0.087 0.085
36.0759494 35.6012658 35.8122363 0.24169703 0.13954385 0.632 0.019 0.02 0.017
40.3481013 40.1898734 40.1898734 0.15822785 0.09135289 0.632 0.014 0.013 0.015
 PH11
HE(R1) HE(R2) HE(R3) AVERAGE SD SE
60.7594937 60.2848101 61.0759494 60.7067511 0.39819802 0.22989973
69.3037975 68.5126582 68.8291139 68.8818565 0.39819802 0.22989973
84.335443 84.8101266 84.9683544 84.7046414 0.32937753 0.19016621
86.0759494 86.2341772 86.5506329 86.2869198 0.24169703 0.13954385
96.9936709 96.835443 97.3101266 97.0464135 0.24169703 0.13954385
97.7848101 97.943038 97.6265823 97.7848101 0.15822785 0.09135289