Nta Gpat 2025 Syllabus
Nta Gpat 2025 Syllabus
Nta Gpat 2025 Syllabus
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PHYSICAL CHEMISTRY
2. Colligative Properties
The liquid state, vapor pressure, ideal & real solutions. Raoult's law, elevation of boiling point,
depression of freezing point, osmotic pressure, determination of molecular weight based on
colligative properties.
3. Thermodynamics
First, second & third law of thermodynamics. Thermochemical laws, isothermic & adiabatic
processes, reversible processes, work of expansion, heat content, enthalpy, heat capacity.
Gibb's & Helmholtz equation & chemical potential.
4. Refractive index
Refractive index, specific refractivity, molar refractivity, refractometers.
5. Solutions
Solubility, factors affecting solubility, solubility curves. Types of solutions, effect of co-solvency,
pH & other factors on solubility. Solubility of gases in liquids, liquids in liquids, & solids in
liquids, critical solution temperature, law of partitioning & its applications. Solute-solvent
interactions. Expression of the concentration of pharmaceutical solutions & calculations.
Molarity, molality, mole fraction & percentage expressions.
6. Electrochemistry
Properties of electrolyte solutions, electrolysis. Faraday's law of electrolysis, electron transport,
electrical cell, single electrode potential, concentration cells, half-cells & half-cell potential,
types of half cells, sign convention, Nernst equation, salt bridge, electromotive series, standard
potential, SHE. Measuring the relative voltage of half cells, Calculation of standard potential.
Reference & indicator electrodes. Standard oxidation-reduction potential.
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7. Ionic equilibrium
Theory of conductivity, equivalent conductance, mobility of ions, specific conductance.
8. Kinetics
Order of reactions, derivation & internal form of rate laws, molarities of reaction,
derivation of rate constants.
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PHYSICAL PHARMACY
5. Dispersion systems
a. Colloidal dispersions: Definition, types, properties of colloids, protective
colloids, application of colloids in pharmacy.
b. Suspensions and emulsions: Interfacial properties of suspended particles
settling in suspension, theory of sedimentation, effect of Brownian movement,
sedimentation of flocculated particles, sedimentation parameters, wetting of
particles, significance of electrical properties in dispersions, controlled
flocculation, flocculation in structured vehicles, rheological considerations,
emulsions: types, theories, physical stability.
6. Complexation
Classification of complexes, methods of preparations and analysis, applications.
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7. Buffer
Buffer equations and buffer capacity in general. Buffers in pharmaceutical systems,
preparations and stability, buffered isotonic solutions. Measurements of tonicity calculations
and methods of adjusting isotonicity.
8. Solubility
a. Miscibility-influence of foreign substances
three component systems;
dielectric constant and solubility,
solubility of solids in liquids
ideal and non-ideal solutions
solvation and association in solutions
solubility of salts in water
solubility of slightly soluble and weak electrolyte
calculating solubility of weak electrolytes as influenced by pH, influence of solvents on the
solubility of drugs
combined effect of pH and solvents, distribution of solutes between immiscible solvents,
effect of ionic dissociation and molecular association on partition, extraction,
preservatives action of weak acids in emulsions, drug action and distribution coefficient.
b. Concepts of dissolution and diffusion.
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ORGANIC CHEMISTRY
1. General principles
3 2
A brief review of classification & sources of organic compounds, sp , sp , sp hybridization,
sigma & pi- bonds, bond lengths, bond angles & bond energies along with their significance in
reactions should be carried out. An overview of bond polarization, hydrogen bonds, inductive
effects, resonance, and hyperconjugation be taken. Concept of homolytic & heterolytic bond
fission, acidity & basicity with different theories should be covered briefly. Ease of formation
& order of stabilities of electron deficient & electron rich species along with the reasons for
the same should be covered. Relationships between energy content, stability, reactivity &
their importance in chemical reactions should be covered. Calculations for determining
empirical & molecular formula should be covered.
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character of a compound. A brief coverage of structure of benzene. Detailed coverage of
electrophilic & nucleophilic aromatic substitution reactions. Reactivity & orientation in these
reactions. Reactivity & orientation in mono- & disubstituted benzenes. Benzyne mechanism.
Aromatic hydrocarbons.
Phenolic compounds.
Aromatic & aliphatic amines.
Diazonium salts.
Aromatic nitro- compounds, aryl halides, & ethers.
7. Carbonyl Chemistry
Carbonyl chemistry involving group conversions & their reaction mechanisms along with
stereochemistry wherever applicable.
a. Wolf-Kishner reduction & Huang-Minlong modification.
b. Reduction of arylsulfonyl hydrazine/hydrazones to alkanes.
c. Bamford Steven reaction.
d. DCC Oxidation of alcohol.
e. Michael addition / 1,4-addition / conjugate addition.
f. Mannich condensation / reaction.
g. Robinson annulation.
h. Stobbe condensation.
i. Darzen's glycidic ester synthesis.
j. Beckmann rearrangement.
k. Baeyer Villiger rearrangement.
l. Curtius, Wolff, & Lossen rearrangements.
m. Willgerodt rearrangement.
n. Pinacol-pinacolone rearrangement.
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o. Methylene transfer reactions. Use of diazomethane & sulphur ylides in the same.
p. Mono- & dialkylations in 1,3-dicarbonyl compounds.
q. Formation & use of enol ethers, enol acetates & enamines as protective groups & in
regiospecific alkylations.
8. Heterocyclic Chemistry
IUPAC Nomenclature of heterocyclic rings [3-10 membered] containing O, S, & N atoms.
Nomenclature of above rings containing mono-, di-, & multiple [same or different] heteroatoms
should also be covered. Nomenclature of 2 & 3 fused rings containing mono-, di-, & multiple
heteroatoms [same or different] should also be covered. Syntheses & reactions of three to six-
membered rings in detail. Syntheses of five & six-membered rings containing mono- or any di-
heteroatoms [O, S, & N]. Syntheses of quinoline, isoquinoline, benzoxazole, benzothiazole, &
benzimidazole, benzotriazole, and benzothiazole.
9. Bridged rings
Bridged ring systems & their nomenclature.C8, C9, C11 bridged bicyclic alkanes. Chemistry of
hexamine, morphan, biperiden, amantadine, diazabicyclo[2.2.2] octane
11. Stereochemistry
Stereochemistry. Chirality & asymmetry [introduction of the same to S, P, & N]. Definition &
classification [different types of isomerisms]. Enantiomers, diastereomers. Enantiomerism &
diastereomerism. Meso compounds & their optical activity. Stereochemistry in acyclic
compounds. Newman projection formulae & their significance. Conformational analysis of n-
butane. Absolute & relative configuration. Assigning R & S configuration based on Cahn Ingold
& Prelog system. Racemic mixture- its definition & resolution. Definitions of terms
stereoselective, stereospecific, Enantiomeric excess & diastereomeric excess. Stereochemistry
in cyclic systems. Conformations of cyclohexane. Cis-trans relationship in cyclohexane.
Prediction of stability of different conformations of 1, 2- 1,3- & 1,4- disubstituted cyclohexanes.
Effect of multiple substitutions on the stability of cyclohexane conformations. Chair
conformations of cis-, & trans-decalins, perhydrophenanthrenes, & a tetracyclic steroidal
nucleus. An introduction to atropisomerism.
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12. Carbohydrates
Carbohydrates. Definition & classification. D & L nomenclature in sugars. Different ways of
drawing / representing a sugar molecule [including cyclic Structure], interconversion of these
representations. Anomers & epimers. Mutarotation. Reactions of glucose. Chain extension &
chain reduction of a sugar.
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PHARMACEUTICAL CHEMISTRY
1. Pharmaceutical Impurities
Impurities in pharmaceutical substances, sources, types & effects of impurities. Limit tests for
heavy metals like lead, iron, arsenic, mercury & for chloride & sulphate as per Indian
Pharmacopoeia [I. P.].
2. Monographs
(a) Monograph & its importance, various tests included in monographs as per I. P. A study
of the following compounds with respect to their methods of preparation, assay, &
pharmaceutical uses of sodium citrate, calcium carbonate, copper sulphate, light &
heavy kaolin, ammonium chloride & ferrous gluconate.
3. Isotopes
Isotopes- stable & radioactive, mode & rate of decay. Types & measurement of radioactivity.
Radiopharmaceuticals & their diagnostic & therapeutic applications in pharmacy & medicine
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such as I, P, Cr, Co, Fe, Tc-M. Radiocontrast media, use of BaSO4 in medicine.
4. Dentifrices, desensitizing agents, & anticaries agents
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wherever applicable, synthesis of simple & prototype molecules, drug metabolism, therapeutic
uses & side effects. Drug resistance, wherever applicable, should be covered in respective
classes of drugs.
a. Antimalarials
b. Antiamoebic agents.
c. Anthelmintic agents.
d. Antibacterial sulpha drugs [only].
e. Quinolone antibacterials.
f. Antimycobacterial drugs.
g. Antifungal agents.
g. Antiviral agents including HIV & anti-HIV drugs.
h. Thyroid & antithyroid drugs.
i. Antiallergic agents.
j. Antiulcer agents & Proton Pump Inhibitors.
k. Hypoglycemic agents.
II. Antibiotics. Penicillins, cephalosporins & other beta-lactam antibiotics like imipenem &
aztreonam. Beta-lactamase inhibitors such as clavulanic acid & sulbactam.
Chloramphenicol. Tetracyclines. Aminoglycoside antibiotics. Macrolide antibiotics.
Lincomycins. Polypeptide antibiotics. Anticancer antibiotics.
III. Steroids. Corticosteroids [gluco- & mineralocorticoids] & anti-inflammatory steroids. Sex
steroids. Male & female contraceptive agents. Anabolic steroids.
Anticancer agents.
8. Different classes of therapeutic drugs
A detailed study of the following classes with respect to drug nomenclature, classification,
physicochemical properties, mode of action [MOA], biosynthesis, structure-activity
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relationships [SAR], wherever applicable, synthesis of simple & prototype molecules, drug
metabolism, therapeutic uses & side effects. Drug resistance, wherever applicable, in respective
classes of drugs.
I. Narcotic [centrally acting] analgesics [analgetics]. Morphine & all its structural
modifications [peripheral & nuclear]. Narcotic agonists & antagonists [dual & pure].
Non-narcotic analgesics [NSAIDS]. Difference between narcotic & non-narcotic agents.
II. Adrenergic drugs. Neurotransmitters & their role. General & specific adrenergic agonists
& antagonists [up to alpha-2 & beta-2 only].
III. Cholinergic agents. Muscarinic & nicotinic cholinergic agonists & antagonists [up to M2
& N2]. Neuronal [transmission] blockers.
IV. Drugs used in neuromuscular disorders. Drugs used in the treatment of
Parkinson's disease. Central & peripheral muscle relaxants.
V. Hypertensive, antihypertensive, & antianginal agents.
VI. Diuretics.
VII. Eicosanoids. Prostaglandins, prostacyclins, & thromboxanes. Their biochemical
role, biosynthesis, & inhibitors.
9. Introduction to quantitative structure-activity relationship. [QSAR]. Linear free energy
relationship. Hammett's equation. Use of substituent constants such as π,
σ, Es, & physicochemical parameters such as pKa, partition coefficient, Rm, chemical shifts,
molar refractivity, simple & valance molecular connectivity to indicate electronic effects,
lipophilic effects, & steric effects. Introduction, methodology, advantages &
disadvantages/limitations of Hansch analysis.
10. Asymmetric synthesis. Chirality, chiral pool, sources of various naturally available chiral
compounds. Eutomers, distomers, eudismic ratio. Enantioselectivity &
enantiospecificity. Enantiomeric & diastereomeric excess. Prochiral molecules.
Asymmetric synthesis of captopril & propranolol.
11. Combinatorial chemistry. Introduction & basic terminology. Databases & libraries. Solid
phase synthesis technique. Types of supports & linkers, Wang, Rink, & dihydropyran
derivatized linkers. Reactions involving these linkers. Manual parallel & automated
parallel synthesis. Houghton's tea bag method, micromanipulation, recursive
deconvolution. Mix & split method for the synthesis of tripeptides. Limitations of
combinatorial synthesis. Introduction to throughput screening.
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PHARMACEUTICS
5. Crude extract
Infusion, decoction, maceration, percolation, tincture and extract. Methods of preparations of
dry, soft and liquid extract.
6. Allergenic extract
Types of allergens, preparation of extract, testing and standardization of extracts.
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7. Biological products
Absorbable and non-absorbable material types, sutures and ligatures, processing,
manufacturing, sterilization, packing, QC tests of materials like catgut and nylon.
10. Powders
Advantages and limitations as dosage form, manufacturing procedure and equipment, special
care and problems in manufacturing powders, powders of IP, effervescent granules and salts.
11. Capsules
Hard gelatin capsules, shell formulation and manufacturing, capsule sizes, storage, filing,
cleaning process general formulation contents and evaluation. Soft gelatin capsules, shell
formulation, formulation contents, filing, sealing and storage. Microencapsulation, advantages,
encapsulation materials, methods of microencapsulation, I.P. formulations
12. Tablets
Types, ideal requirement, classification, granulation methods, general formulation,
compression machines, different types of tooling’s, difficulties in tableting, troubleshooting
aspects, evaluation, sugar coating, compression coating, film coating, problems in tablet
coatings and their troubleshooting aspects. IP formulations.
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Formulation of deflocculated and flocculated suspension, manufacturing procedure, evaluation
methods, IP suspensions.
15. Emulsions
Types, emulsifying agents, general formulation, manufacturing procedure, evaluation methods,
IP emulsions.
16. Suppositories
Ideal requirements, bases, manufacturing procedure, evaluation methods, IP products.
17. Semisolids
Definitions, bases, general formulation, manufacturing procedure, evaluation methods, IP
products.
18. Liquids (solutions, syrups, elixirs, spirits, aromatic water, liquid for external uses)
Definition, types, general formulation, manufacturing procedure, evaluation methods, IP
products.
21. Preformulations
Consideration of Importance, physical properties, physical forms, particle size, crystal forms,
bulk control, solubility, wetting, flow cohesiveness, compressibility, organoleptic properties and
its effect on final product consideration of Chemical properties, hydrolysis, oxidation,
recemization, polymerization, isomerization, decarboxylation, enzymatic decomposition,
formulation additives, stabilizers, suspending and dispersing agents dyes, solid excipients etc.
and its effect on quality of finished product.
22. Stability of formulated products
Requirements, drug regulatory aspects, pharmaceutical products stability, shelf life, overages,
containers, closures.
Reaction rate and order, acid-base catalysis, destabilization and accelerated stability testing.
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23. Prolonged Action Pharmaceuticals
Benefits, limitations, oral products, terminology, drug elimination rate, types and construction
of implants products, product evaluation, parenteral products, absorption and evaluation.
27. Cosmetics
Formulation and preparation of dentifrices, hair creams, lipsticks, face powders, shaving
preparations, skin creams, shampoos, hair dyes, depilatories, manicure preparations etc.
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PHARMACOLOGY
1. General Pharmacology
Introduction to Pharmacology- Definition, scope and source of drugs, dosage forms and routes
of drug administration. Pharmacodynamics-Mechanism of drug action, Receptors,
classification and drug-receptor interactions, combined effects of drugs, factors modifying
drug actions.
Pharmacokinetics-Mechanism and principle of absorption, distribution, metabolism and
excretion of drugs. Principles of basic and clinical pharmacokinetics. Pharmacogenetics.
Adverse drug reactions.
Discovery and development of new drugs-Preclinical and clinical studies.
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Anti-hypertensive drugs, Anti-anginal agents, Anti-arrhythmic drugs.
Drugs used in congestive heart failure. Anti-hyperlipidemic drugs. Drugs
used in the therapy of shock.
Haematinics, anticoagulants and haemostatic agents.
Fibrinolytics and antiplatelet drugs.
Blood and plasma volume expanders.
9. Chemotherapy
General principles of chemotherapy. Sulphonamides and co-trimoxazole.
Antibiotics- Penicillins, cephalosporins, chloramphenicol, Macrolides, quinolones and
fluoroquinolons,. Tetracyclines. Aminoglycosides and miscellaneous antibiotics.
Chemotherapy of tuberculosis, leprosy, fungal diseases, viral diseases, AIDS, protozoal
diseases, worm infections, urinary tract infections and sexually transmitted diseases.
Chemotherapy of malignancy.
12. Chronopharmacology
Definition of rhythm and cycles. Biological clocks and their significance leading to
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chronotherapy.
13. Immnopharmacology
Immunostimulants and immunosuppressants.
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PHARMACOGNOSY
1. Introductory Pharmacognosy
Historical development, modern concept and scope of Pharmacognosy. The significance of
Pharmacognosy in various systems of medicine practiced in India viz: Ayurveda, Unani,
Homeopathic and Siddha.
4. Techniques in microscopy
Details of mountants, clearing agents, chemomicroscopic (microchemical) reagents.
5. Introduction to phytoconstituents
Definition, classification, chemical tests and pharmaceutical importance of: carbohydrates and
their derivatives, fats and proteins, alkaloids, glycosides, flavonoids, steroids, saponins, tannins,
resins, lipids and volatile oils.
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Pteridophytes: Male fern.
7. Pharmaceutical aids
Biological sources, chemical constituents, adulterants and uses of: Starches, acacia gum,
tragacanth, sterculia, guar gum, pectin, arachis oil, castor oil, sesame oil, cottonseed oil, olive
oil, cotton, silk, wool, regenerated fibers, asbestos, kaolin, prepared chalk, kieselguhr.
8. Animal products
Biological sources, chemical constituents, adulterants and uses of: Shellac, cochineal,
cantharides, woolfat, lard, beeswax, honey, musk, lanolin, gelatin.
9. Plant products
Introduction to plant bitters, sweeteners, nutraceuticals, cosmeceuticals and photosensitizing
agents.
11. Enzymes
Biological sources, preparation, characters, and uses of: diastase, papain bromelain, ficin, yeast,
pancreatin, urokinase, pepsin, trypsin, penicillinase, hyaluronidase and streptokinase.
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15. Biogenetic pathways
Formation of primary and secondary metabolites. Study of Calvin cycle, TCA cycle, Shikimic acid
pathway, Embden-Mayerhoff pathway, acetate hypothesis, isoprenoid pathway. Biosynthesis of
carbohydrates, lipids and volatile oils.
17. Tannins
Biological sources, morphology, chemical constituents, chemical test and uses of: Pale catechu,
black catechu, nutgalls, Terminalia belerica, Terminalia chebula, Terminalia arjuna.
20. Glycosides
Nature and classification. Biological sources, morphology, chemical constituents, adulterants
and uses of: Digitalis, strophanthus, squill, thevetia, oleander, cascara, aloe, rhubarb, senna,
quassia, dioscorea, quillaia, glycyrrhiza, ginseng, gentian, wild cherry, withania, bitter almond.
Biosynthesis of cardiac and anthraquinone glycosides.
21. Alkaloids
Nature, classification, biological sources, morphology, chemical constituents, adulterants and
uses of: Areca nut, belladonna, hyoscymous, stramonium, duboisea, coca, coffee, tea,
cinchona, opium, ipecac, nux vomica, ergot, rauwolfia, vinca, kurchi, ephedra, colchicum,
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vasaca, pilocarpus, aconite, Solanum xanthocarpum. Biosynthesis of tropane, cinchona and
opium alkaloids.
23. Phytopharmaceuticals
Isolation, identification and estimation of: caffeine, eugenol, digoxin, piperine, tannic acid,
diosgenin, hesperidin, berberine, calcium sennosides, rutin, glycyrrhizin, menthol, ephedrine,
quinine, andrographolides and guggul lipids.
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microphylla), lehsun (Allium sativum), guggul (Commiphora mukul), kalmegh (Andrographis
peniculata), tulsi (Ocimum sanctum), valerian (Valerian officinalis), artemisia (Artemisia annua),
chirata (Swertia chirata), ashoka (Saraca indica).
30. Patents
Indian and International patent laws, proposed amendments as applicable to herbal/natural
products and processes: Intellectual Property Rights with special reference to
phytoconstituents.
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PHARMACEUTICAL ANALYSIS
2. Acid-base titrations
Definitions of acids & bases according to Arrhenius & Lewis theory. Definitions of normality,
molarity, molality, & equivalent weight. Primary & secondary standards with examples &
differences between them. Standardization of strong acids & bases using primary & secondary
standards. Preparation of standard solutions of & calculations of equivalent weights of oxalic
acid, potassium acid phthalate, calcium chloride dihydrate, & sodium carbonate. Calculation of
factors involved in standardization of sodium hydroxide, hydrochloric acid, & oxalic acid. Direct,
back & differential titrations. Application of direct & back titrations to preparations like boric
acid & borax in a mixture, ammoniated mercury, milk of magnesia, & zinc oxide ointment.
Law of mass action, acid-base equilibria, pH scale, pH & hydronium ion concentrations in
aqueous systems, calculations of pH for weak acids & weak bases. Use & applications of pH
meter. Hydrolysis of salts. Strengths of acids & bases, dissociation constant.
Definition, different types of buffers [chemical & biological], & their composition. Buffer
capacity, buffered isotonic solutions. Calculations involving preparation of various buffer
capacity solutions. Biological & pharmaceutical applications of buffers.
3. Non-aqueous titrations
Acid-base definitions according to Lowry-Bronsted, Lewis & Arrhenius concept. Factors
affecting strengths of acids & bases. Intrinsic structure & surrounding environment. Protophilic,
protogenic, amphiprotic & aprotic solvents. Acid-base equilibria in non- aqueous media.
Titrants & indicators used for the assay of acidic & basic substances. Preparation of perchloric
o o o
acid, formation of onium ion. Assay of 1 , 2 , 3 amines & amine hydrochlorides using
perchloric acid & the reactions involved in it. Standardization of sodium ethoxide solution.
Assay of phenols & phenobarbitone. General applications of non-aqueous titrations
4. Oxidation-reduction titrations
Definition of oxidation, reduction, oxidizing & reducing agent. Equivalent weight, the concept of
half reactions. Systematic balancing of half reactions with respect to:
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a. Oxalic acid-KMnO4,
b. FeSO4-ceric nitrate, &
c. I2-sodium thiosulphate solution titrations.
Calculation of equivalent weight of oxalic acid, KMnO4, FeSO4, permanganate & I2 from half-
reactions. Calculation of factors for titrations mentioned in a, b & c.
a) Redox titrations:
KMnO4 as a self indicator, it's preparation, standardization, & use in the assay of ferrous
gluconate tablets, H2O2, & NaNO2 solution.
b) Iodimetric & iodometric titrations. Definitions & difference between iodimetry &
iodometry. Preparation, standardization of iodine solution. Assay of ascorbic acid &
sulphur ointment by iodimetry. Assay of copper sulphate & ferric chloride by iodometry.
c) Bromometric titrations.
d) Iodate titrations. Definition. Preparation, standardization & use of KIO 3 in the assay of
ascorbic acid & KI.
e) Cerimetric titrations. Preparation, standardization & use of ceric solutions in the assay
of paracetamol tablets. Its advantages over permanganate solutions.
f) Bromine titrations. Preparation, standardization & use of bromine solution in the assay
of phenol & isoniazid tablets.
g) Potassium dichromate titrations. Preparation, standardization & use of potassium
dichromate solution in the assay of ferrous ammonium sulphate.
5. Precipitation titrations
The principle of solubility product & sparingly soluble salts.
Titrants & indicators used in Mohr's, Volhard's, & Fajan's methods. Preparation &
standardization of silver nitrate & ammonium thiocyanate solutions. Assay of sodium chloride
by Mohr's method, use of nitrobenzene in the assay of halides, ammonium chloride, & thiourea
by Volhard's method. Calculation of factors in argentometric titrations. Titration curve method.
General applications of precipitation titrations.
6. Complexometric titrations
Difference between double salts & co-ordinate compounds. Definitions of coordination number
of metal ions, ligands- uni-, bi-, & multidentate. Complexing, chelating, & sequestering agents
with respective examples. Structure of complexes of platinum with ammonia. Ethylenediamine
tetraacetate [EDTA] as a multidentate ligand in complexometry. Coordinate compounds of
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EDTA with bi-, tri-, & tetravalent metal ions. Stability of complexes & factors affecting it, use of
buffers in EDTA titrations. Selective analysis of ions based on pH adjustments, use of masking &
demasking agents, pM or metal ion indicators. Standardization of EDTA solution, titration
curves, and examples of assays carried out by direct & back titrations & by replacement of one
complex by the other. Applications of complexometry in the assays of calcium gluconate, milk
of magnesia, zinc undecenoate ointment, & aluminium hydroxide gel. Assay of NaF by indirect
titration.
7. Gravimetry
Principles of gravimetry. Factors affecting precipitation, formation, & properties of the
precipitate. Colloidal state. Impurities in the precipitate, conditions of precipitation.
Precipitation from homogenous solutions, washing, drying, & ignition of the precipitate.
Experimental techniques of drying & ignition. Applications of gravimetry in pharmacy.
8. Extraction techniques
Liquid-liquid extraction, separation of mixtures by extraction. Distribution law. Successive &
multiple extraction [Craig method], continuous counter- current extraction. Effect of
temperature & pH on extraction. Inert solute, associate ion pair formation, emulsion problem
in extractions. Applications in pharmacy.
9. Potentiometry
Theory, ion-selective electrodes, measurement of potential, red-ox titration curve, pH
measurement, the relation of pH to potential. Applications in pharmacy.
11. Calibration
Calibration of instruments.
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Electromagnetic spectrum. Absorption & emission spectroscopy. Understanding of terms such
as absorbance, transmittance, absorptivities, molar absorptivity, E 1cm 1%,
λmax, the effect of solvent & pH on λmax.
14. Spectrofluorimetry
Principle, definitions & types of luminescence. Mechanism of fluorescence & phosphorescence.
Singlet & triplet states & intersystem crossing. Fluorescence yield & factors affecting it.
Quenching of fluorescence & fluorescence quenchers. Structure & fluorescence. A brief
discussion of instrumentation. Applications of fluorimetry in pharmacy.
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[without derivation of the equations]. Types of relaxation processes. Low & high-resolution
instruments. A brief discussion on the low-resolution instrumentation [60 MHz]. Quantitative
knowledge of the relationship between MHz & magnetic field. An introduction to
superconductivity magnets. Solvents & reference standards used. Setting up of NMR scale.
Sample preparation. Shielding & deshielding of a proton & its effect on chemical shifts.
Discussion on & importance of equivalent & non equivalent protons [number of signals],
chemical shifts [position of signal] & their calculation from the spectrum, chemical shifts of
different H's, splitting [multiplicity] of a signal, coupling constants [J values] , integration [area
under the signal]. Importance of these terms in identification [or confirmation] of different
functional groups. Significance & contribution of J value in stereochemistry. Prediction
[expected theoretical values] of chemical shifts & multiplicities for all protons from simple
structures containing up to 12-15 carbons. An introduction to FT-technique & its significance in
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C-NMR spectrometry.
19. Polarography.
Principle & instrumentation. Ilkovich equation [no derivation] & its importance. Dropping
mercury electrode [DME], saturated calomel electrode. Liquid-liquid junction potential,
polarographic cell. Explanation of origin of the S-shaped C-V curve. Applications
of this technique. Amperometric titrations, principles, instrumentation, & applications.
21. Chromatography.
Principle, rate & plate theory, Van Deemter equation & the parameters affecting
separation/band broadening. Classification of chromatography, retention factor. A detailed
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study of thin layer chromatography [TLC], preparative TLC, paper chromatography [PC], column
chromatography, gas chromatography [GC / GLC].
22. Miscellaneous
An introduction to electrophoresis. An introduction to lasers & masers.
Statistical treatment to experimental data. Sampling techniques & applications in
pharmaceutical industry.
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BIOCHEMISTRY
1. Cell
Revision of ultrastructure of the cell, functions of various cellular constituents. Applications of
biochemical principles to the pharmacy.
2. Carbohydrates
Types of carbohydrates, their functions, digestion, & absorption. Aerobic & anaerobic oxidation
with energetics. Glycogenesis, glycogenolysis, & gluconeogenesis. Hexose monophosphate
shunt [HMP shunt]. Diseases associated with carbohydrate metabolism.
3. Proteins
Different types of proteins. Their functions, digestion & absorption. Denaturation & its effect on
biological activity. Renaturation of proteins. Urea formation, urea cycle, creatinine formation.
Transamination & deamination. Proteins as enzymes.
4. Lipids
Different types of lipids. Their functions, digestion, absorption & metabolism. (Beta-Oxidation
of fatty acids with energetics. Biosynthesis of cholesterol [from acetate], adrenocorticoids,
androgens, progesterone, estrogens, & bile acids / salts. Ketone bodies, their formation &
biochemical significance. Diseases associated with lipid metabolism.
5. Vitamins
Definition. Classification, structures [except B12] biochemical role, sources, daily requirements,
& deficiency symptoms. Vitamins as co-factors in biochemical reactions.
7. Enzymes
Classification & their various roles. Enzyme co-factors. Enzyme kinetics. Michaelis-Menton
equation along with its transformations. Double reciprocal plot. Factors affecting enzyme
action. Enzyme inhibition, competitive & non-competitive, & kinetics.
8. Nucleic acids
Different types of nucleic acids [NAs] & their composition. Purine & pyrimidine bases, sugars, &
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phosphoric acid. Nucleosides & nucleotides. Formation of NAs & their backbone. Different ways
of representing DNA & RNA molecules. Physico-chemical properties of NAs. Their stability in
acidic & basic solutions. Isolation, purification & identification, buoyant density, sedimentation
coefficient, & Svedberg constant of NAs. De-novo biosynthesis of NAs. DNA & the Watson-Crick
model & its features. DNA as
the bearer of genetic information. The Central dogma of molecular genetics & the processes
defined in the same. Replication of DNA. Different types of RNAs with their special features &
functions. Minor or rare bases. Transcription & translation. Different post-translational
modifications of proteins. Triplet codon & the codon dictionary. Mutations. An introduction to
different types of mutations. Their nature & repair.
9. Hereditary diseases.
Eliptocytosis, spherocytosis, HNPCC, diabetes insipidus.
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BIOTECHNOLOGY
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Expression of foreign genes.
Uses of recombinant DNA.
Removal of an altered form of the protein of interest from the product stream.
Determination of protein concentration.
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MICROBIOLOGY
1.Introduction to Microbiology
Scope and application to pharmacy field. Whittaker's Five Kingdom concept, historical
development - biogenesis Vs. abiogenesis, Germ theory of fermentation, Germ theory of
disease, the contribution of Leeuwenhoek, Robert Koch, Jenner, Louis Pasteur and Ehrlich.
3.Biology of Microorganisms
Cultural characteristics, pure culture techniques
a) Bacteria - Morphology and fine structure of bacteria, Nutritional requirement and type of
culture media, growth and growth curve of bacteria, physical condition for growth,
measurement of bacterial growth (Counting Methods), Reproduction in bacteria, genetic
exchange - transformation, conjugation, and transduction, development of drug resistance by
recombination and mutation, preservation of bacterial culture. Biochemical properties (sugar
fermentation and IMVIC test). Pathogenesis of Staphylococcus, Mycobacterium. Salmonella
Introductory study of disease-causing rickettsia, the importance of actinomycetes in antibiotic
production.
5.Aseptic Technique
The omnipresence of microorganisms, the importance of asepsis, sources of contamination and
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methods of prevention, Principle, construction & working of laminar airflow bench.
7.Microbial spoilage
Types of spoilage, factors affecting spoilage of pharmaceutical products.
10.Microbial Assay
Importance, general methods of assay of antibiotics (Cup & plate method, paper disc method,
turbidometry, dilution method), methods for fungicidal & antiviral compounds, assay, microbial
limit tests.
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PATHOPHYSIOLOGY
a) Host parasite Relationship: - normal microbial flora of human body, infection vs.
disease, Pathogenicity vs. Virulence, Koch & Rivers Postulates, Reservoir of
infection-sources of infection, Portals of Entry, Portals of exit, vectors of infection,
communicability of disease, recognized symptoms of microbial disease,
classification of immunity.
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Hypersensitivity and its types
Cellular immunity: Transplantation immunity, Cellular immunity to viruses, Implications of
T-cell response. Acquisition of specific immunity: Natural vs. Passive acquisition.
Mechanism of autoimmunity. Classification of autoimmune diseases in man.
Transplantation and allograft reactions, mechanism of rejection of allograft.
Acquired Immune Deficiency Syndrome (AIDS).
6. Infectious diseases
Hepatitis - Infective hepatitis.
Sexually transmitted diseases (syphilis, gonorrhea, HIV). Pneumonia, typhoid,
urinary tract infections. Tuberculosis. Leprosy. Malaria. Dysentery (Bacterial and
amoebic).
7. Neoplastic diseases
Disturbances of growth of cells. General biology of tumors, differences between
benign and malignant tumors. Classification of tumors. Historical diagnosis of
malignancy. Etiology and pathogenesis of cancer. Invasions, metastasis, patterns of
spread of cancer. Environmental carcinogenesis.
9. Laboratory tests for Liver function tests and kidney function tests
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BIOPHARMACEUTICS AND PHARMACOKINETICS
1.Bio-pharmaceutics
a) The fate of drug after drug absorption, various mechanisms for drug absorption, drug
concentration in blood, biological factors in drug absorption, physicochemical factors,
dosage form consideration for gastrointestinal absorption.
b) Drug Absorption:
Gastrointestinal absorption-biological considerations.
Gastrointestinal absorption - physicochemical considerations.
Gastrointestinal absorption-role of the dosage form.
Pharmacokinetics. Compartmental and non-compartmental pharmacokinetics.
Biotransformation, drug disposition - distribution, drug disposition - elimination.
Variability-Body weight, age, sex and genetic factors. Pharmacokinetic variability-
diseases. Pharmacokinetic variability-drug interactions. Individualization and
optimization of drug dosing regimens.
2.Bio-availability & Bio-equivalence
Quality parameters of dosage forms. Assay methods & its validation.
Physicochemical properties of drugs & added substances and its effect on preparations and
biological availability of dosage forms. Pharmaceutical properties of dosage forms,
disintegration, dissolution rate. Biological, pharmacological effects of dosage forms. Factors
affecting Bioavailability, Determination of bioavailability.
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CLINICAL PHARMACY AND THERAPEUTICS
1. Cell physiology
Cell, Cell junctions, transport mechanisms, homeostasis, ion channels, secondary messengers.
2. The Blood
Composition and functions of blood, RBC, WBC, platelets. Homeostasis, blood groups,
mechanism of clotting. Introduction to disorders of the blood.
3. Gastrointestinal tract
Structure of the gastrointestinal tract, functions of its different parts including those of liver,
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pancreas and gallbladder, various gastrointestinal structures and their role in the digestion and
absorption of food.
4. Respiratory System
Structure of respiratory organs, functions of respiration mechanism and regulation of
respiration, respiratory volumes and vital capacity.
6. Sense organs
Structure and physiology of eye (vision), ear (hearing), taste buds, nose (smell) and skin.
7. Skeletal System
Structure and function of the skeleton. Articulation and movement. Disorders of bones and
joints.
9. Urinary System
Various parts Structure and functions of the kidney and urinary tract. Physiology of urine
formation and acid-base balance. Brief Introduction to disorders of the kidney.
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Anatomy of heart and blood vessels, physiology of blood circulation, cardiac cycle, conducting
system of the heart, heart sound, electrocardiogram, blood pressure and its regulation.
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PHARMACEUTICAL ENGINEERING
1. Fluid flow
Type of flow, Reynold's number, viscosity, the concept of the boundary layer, basic equation of
fluid flow, the study of valves, flow meters, manometers and measurement of flow and
pressure including mathematical problems.
2. Heat transfer
Source of heat, mechanism of heat transfer, the laws of heat transfer, steam and electricity as
heating media, determination of requirement of the amount of steam/electrical energy, steam
pressure, boiler capacity, mathematical problems on heat transfer, steam traps and reducing
valve, lagging etc.
3. Evaporation
The basic concept of phase equilibrium, factors affecting evaporation, evaporators, film
evaporators, single effect and multiple effect evaporators, mathematical problems on
evaporation.
4. Distillation
Raoult's law, phase diagram, volatility: simple steam and flash distillation, principles of
rectification, Mc-Cabe Thiele method for calculations of a number of theoretical plates,
azeotropic and extractive distillation, mathematical problems on distillation.
5. Drying
Moisture content and mechanism of drying, the rate of drying and time of drying calculations,
classifications and types of dryers, dryers used in pharmaceutical industries and special drying
methods like freeze drying and lyophilization, mathematical problems in drying.
7. Extraction
Theory of extraction, extraction methods, equipment for various types of the extraction
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process.
8. Mixing
Theory of mixing, solid-solid, solid-liquid and liquid-liquid mixing equipment.
9. Crystallization
Characteristics of crystals like purity, size, shape, geometry, habit, forms, size and factors
affecting them. Solubility curves and calculation curves and calculations of heat balance around
S Swanson's Walker crystallizer , supersaturation theory and its limitations, Nucleation
mechanism, crystal growth, study of various types of crystallizers, tanks, agitated batch,
Swanson’s Walker, single vacuums, circulating magma and crystal crystallizers, cracking of
crystals and its prevention. Numerical problems on yields. Introduction to polymorphism.
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12. Refrigeration and air conditioning
Principles and applications of refrigeration and air conditioning.
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PHARMACEUTICAL MANAGEMENT
1. Introduction to management
Types of management. Basic concepts of management, management process, function and
principles. Levels of management, pharmaceutical management art, science or profession.
Social responsibilities of management, functions of management.
3. Organization
Definition, nature, theories, functions, line and staff organization concepts.
4. Research Management
R & D organizations and research categories. Elements needed for an R & D organization.
Technology transfer.
5. Inventory Management
Objective and functions of inventory control. Types of inventories. Requirements of effective
inventory control.
6. Communication
Nature, types of communication, process, channels and barriers of communication. Limitations
of communications. Importance in pharmaceutical industries.
7. Marketing Research
New product selection, product management, advertising.
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Definition, HRD methods, HRD process, HRD in Indian industry.
10. GATT
General Agreement on Tariff and Trade and its impact on the pharmaceutical industry. History
of GATT, its impact on the pharmaceutical industry. Pharmaceutical market in
India.
11. World trade organization (WTO) and trade-related intellectual property rights
(TRIPS)
Introduction to WTO. Types of intellectual property rights: industrial property and copyrights
Indian Patent Acts, 1970 with the latest amendment. Definition, types of patents.
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PHARMACEUTICAL JURISPRUDENCE
3. Drugs and Cosmetics Act 1940, Rules 1945, including New Drug applications.
6. Medicinal and Toilet Preparations (Excise Duties) Act 1955, Rules 1976.
15. Introduction to Intellectual Property Rights and Indian Patent Act 1970.
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DISPENSING & HOSPITAL PHARMACY
Antihypertensive drug
Antiamoebic drugs
Antihistaminic drugs
Antiemetic drugs
Antacids and ulcer healing drugs.
Antidiarrheals and laxatives
Respiratory drugs
Antibiotics
Analgesics and antipyretic drugs.
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Inhalations
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