BÍOBAS6

Cmitcnt
1 H-lítVk. ............-,............'...........„.,,-.,..,...................................,................................................,............................................-...................................------*..................-............-..................................

Contení..................................................-......................................,........................................................................................2
1. Unpacking, ínstailaíion, Debuggiiig..................................................................................................................................3
í.l Unpacking.....................................................................................................................................................................3
1.2 Accessories cliecking..................................................................................................................................................5
1.3 Installalion conditions and using environtncr.í............................................................................................................5
1.4 Instaliuiiütt....................,.............................................................................................................................................6
1.5 Checking aftei: inslallaüon........................................................................................................................................¡0
2. Üser Insíructians..............................................................................................................................................................! i
2.1 Fundióos....................................................................................................................................................................11
2.1.1 Product Concept.............................................................................................................................................. lí
2.1.2 Application Rango...........................................................................................................................................!1
2.1.3 Working theory/Air flow pattern and protected arca...............................................................................*.....11
2.1.4 Protected objects.................................................................................................................................,............12
2.1.5 Tcchníca l parame ters......................................................................................................................................12
2.1.6 Performance Index..........................................................................................................................................12
2.2Produclsiructure........................................................................,...........................................................,...................13
2.2.1 StrueturalcompositionorU231 BBC 86...........................................................................................................13
2.2.2 Structure introductíon......................................................................................................................................14
2.3 Control panel..............................................................................................................................................................16
2.4 Remote Control &Foot switch................................................................................................................................... 17
2.5 ínstructions fox Operation,........................................................................................................................................19
2.5.1 Normal Operation Notice................................................................................................................................19
2.5.2 Operation Process................................................................................................................'...........................20
2.6 Daily maintenance..............................................,.....................................................................................................20
2.6.1 Preparalíons before maintenance.............................,.......................................................................».............20
2.6.2 Clean the cabinet surfacc.................................................................................................................................21
2.6.3 Overail maintenance period..............................................................,............................................................21
2.6.4 Maintenance methods......................................................................................................................................21
2.6.5 Storage conditions...........................................................,..............................................................................21
2.7 Methods and procedures for disinfection..................................................................................................................22
2.8 Repiacement parts list...............................................................................................................................................24
2.9Wírmgd¡agram...........................................................................................................................................................25
3. Trouble shooting and Labels............................................................................................................................................27
3.1 Common faulís & solution...................................................................................................................,....................27
3.1.1 Warning and reminder.....................................................................................................................................27
3.1.2 Trouble shooting..............................................................................................................................................27
3.1.3 Simple accessoríes repiacement......................................................................................................................29
3.2 Label Description................................................................................................................................■....................31
4. Warranty and repair senices.............................................................................................................................................33
 Preface
Thank yon ver>r much for purchasing our class IIA2 biológica] safety Cabinet. Please read Ihe "User
manual" and íhe related documenta beibre operating this unit to assure proper operaíion. After
reading these documents, be sure to store tíiem securely together ai a hand place for future rcfcrence.

A
' ' ^ Warning: Before operating the unit, be sure to read carefully and ñiliy understand irnporrant
wamings in íhe operating instractions.
 -i. l_- «I jjít Civllij^, iiijiuliúuun, (j i_i> tí iiildl il

Picase fírsíiv check íf'packing box is m good condstion. If the paekíng box is damaged, picase take
pilotos.

1.1 Unpacking

Choose the proper unpacking method accordíng to the actual situation.

a. Forwooden box:
1) Method í: Necessary tooís íor unpacking: Eíccíric drill with hexagon dead M8

Picture 1
2) Method 2; Use M8 Wrench to unpack
b. For cartón box:
Using ordinaty scissors to cut packing tape, take off íhe package cover, then move up the paper box
body,
 ],?. Acechones ehod-:in£

Refer. to ihepacking list a tic! cheek ihe aeeessories,

Packinglist (11231 BBC 86)
NO. ítems Quaníity
1 Main Body 1 unit
2 Base Stand 1 set
3 UVLampfTS ]5W) ipes
4 Fusc(lOA) 1 pes
5 Fuse (5A) 1 pes
6 Remóte control (including battery) 1 pes
7 Keys 2 pes
8 User manual 1 pes
9 Test report 1 pes
10 Quality certifícatión card 1 pes
11 Base stand 1 set
12 Inner hexagon cylinder head screws M10*20 4 pes
13 Plaín washer 10 4 pes
14 Spring vvasher 10 4 pes
15 inner hexagon wrench í pes
16 Power cord 1 pes
17 Drain Valve 1 set
18 Big rubber gasket 1 pes
19 Small mbbcr gasket 1 pes
20 Front vvindow tubular motor control rod 1 pes
21 Drain baffle 1 + drain baffle II + plaín 1 sel
washer 6 4- spring washer 6 + M6 mil

1.3 Installation conditions and using environment

To avoid disturbances to íhe salety cabinet and its operator, follow the followíng guideímes, while
determining a suitabic location for the cabinet:
a. The distance from the plañe of the aperture to any círcuíation space shotild be at least 1000 ram,
so as to preserve a zone undisturbed by anyone other than the opei'ator.
b. BioíoaJcai saieiy cabmets shouid be placed in a posiiion wiicre tnere shouid be no opposing waii

c. Safety cabínets shouid nol be inslalled í.n positions where they are iíkely lo be affected by oiher
Ítems or equipmení. ín particular íhe distance to the apernare of an opposing safely eabinet, fume
cupboard, or ihe edge of a local exhaust vetitiiation ouüet shouid not be less íhan 3000 mm.
d. Anyroom atr supply diffuscr shouid not be within 1500 mm ofthe íront aperlure.
e. Doorways shouid not be within 1500 mm ofthe apert tire orwithin 1000 mm ofthe side ofthe
safety cabinel.
f The position of a safety cabi.net shouid satisfy the spatial reqniremants (e.g. visión, lightíng and '
conveníence of access) of tlie operator and personnel working nearby. When a cabínet is
inslalled on a bench top, ihe leading edge shouid be flush vvith or sligbtly overhanging thc edge
of me bench top.
Working environment:
a. Only is suiíable íor indoor.
b. Ambient temperatura: Í5°C~35CC,
c. Relarive Humidity: <75%.
d. Atmospheric pressure ránge: 70kPa~I061d?a.
e. Eléctrica! parameters: Consístent with the rated voltage oí the biosafety eabinet (See 2.1.5
techmcal parameter performance índex).
f. Power supply need to be grounded. (Judging niethod: íesting the fíre wire and the zero line of
the power supply with multimeter, thc fire wire to «round voltage shouid be grid voltage and the
zero line lo ground voltage shouid be 0, otherwise the power supply ground ís bad).
g. Test the voltage stability before using, if the voltage is unstable, shouid use íhe voltage regulator,
Oílierwise thc control panel and transformar may be easiiy damaged.

1.4 Installation

a. Remove ail thepackage modules.

b. Inspcct the surface of main body whether there is serateb, deformation or íbreign matters.
c. Movc the wholc device to the final instaüation location,
d. The base stand (optíonal), drain valve assembly.
According to the operating platform which the biosafety eabinet will be placed, lo judge the biosafety
eabinet without base stand, if the drain holc has reserved installation space, to detennine the
installation of drain valve or drain baffle.
/•\ Note: Support seclion at thc time of transporl on the back of the safety cabinel, ií should be
removed before insíaUatton, it is forbidden to the bottom up and place or rcmove dumping. Referring
to picture 5 assemble Üie base stand.

Picture 5
1. Leftside bracket. 3. Connecting píate
2. Righí sidebrackeí 4. M 10*20 Inner h ex agón cylinderhead screws
Remove the MI0*20 Innerhexagon cylinderhead screws from the accessory box, from the left side
bracket and righí side bracket oí' the base stand to inset screw, for connecting with the connecting
píate, Ihen tighten the fíx using inner hexagon wrench, referring to picture 5.

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¿—*.-* Note: Thc base moimfíng bolfs are fixed to thc base stand. Wheii install thc baso stand, except llie 4 bolts marked
with red chele in picture 5, picase remove the other holts and insta)!.

e. Coimect base stand and main body

Refer to picture 6 to connect base stand and main body.
 Fi erare o Rcmove the M 10x20 Inncr hexagon
cylinderhead screws, the MÍO Flal washer and tbe MÍO Spring washer from the accessory box.
Fasten tightly according to pichare 6.
f. Instaliation of Drain valve

Picture 7
1. Drain valve connect
2. Big rabber gasket (Inner díaineter*outer diaraeter*tbicknessO20*O2S*2mm)
3. Safety cabinet bottom instaliation boles
4. Ball coupling fastening nut
5. Sraall rubber gasket (Inner diameter*outer diarneter*thickness0>13*<l>l9*2mra)
6. Drain valve
 1. FasíeningMut
2. Stainless Steel Water and Gas Taps
Take out fastening nuts, water and gas taps, installing as picmre 10.

1.5 Checking after installation

First, make sure the Voltage and frequcncy to be same as logo showing, and then check me follows
Ítems witíi power on:
Checking ítems Non-nal situation

Wind speed display Inftow 0.53±0.025m/s , downflow 0.33±0.025m/s

Pressure display exhaust filler 80-1 lÜPa, downflow filter 80-110Pa
Fan running Nomially
LED larap Lamp lights añer pressing button
UV Lamp Lamp lights after pressing button
Display screen buttons Alt bmíons can be used
Socket Press the socket key. mulíimeter testing output supply voltage
Foot Switch Red pedal for glass up, black pedal for glass down
 Take out drain vaíve couphng, big rubber gasket. baíl nvit. small mbber í»asket. drain valve. g.

Drain baffle Installation

Picture S
1. M6nut 4. Shira
2. Spring washer 6 5. Safety cabinet bottom installation boles
3. Drain boards I 6. Drain boards II
Take out drain boards I, drain boards 11, Shim, spring washer 6, M6 nut, assembling from up lo down
as picture S illuslrated.
h. Adjustment of Footmaster Caster

Picture 9 Clockwise roíate caster' red part to low down

the base feet and the height of the cabinet Low down all four casters can move íhe cabinet position.
Counterclockwise rotate casters' red part can rise the base feet and height of the cabinet. Raise all four
casters can at same time can fix the cabinet. Adjust the four Footmasters makes the cabinet stable.
 2.1.1 Product Concept
This producís belong lo Cjass IIA2 biológicas safety cabinet vvhicíi fully mect. the requirement of US
standard ANSI'NSF49:2002, European standard EN12469:2000, biológica! safety cabinet is a kind of
negative pressure fíltrarion sysíem for proíecting opcrator, the laboratory environment and work
materials, the front opening which air flow inward have proteclion funetion for operaíor, the filtered
laminar flow gencrated by vertical HEPA can protect work materials, wbafs more, the polluted air
flow beeorae puré after processed by HEPA(ULPA) fílter. When it's used in raicrobiology
experimení environment fiíled vvíth vola tile or ioxic chemical and radioiiuclide, suitable exhausl
hood in funetion have to be íinked.
2. i .2 Application Range
Biologíeal Safeíy Cabinet is necessary equipraent in the laboratory in the search of microbiology,
biomedical, DNA recoinbinant, animal experiment, and biológica! producís, especially in the
occasion ihat operator necd to adop! protective measure, such as medical and healtíi, phannacy,
medical research, Our equipmenl provides a safety working environment which don't have bacterial
and dust in the process of bacterial culture.
2.1.3 Working theory/Air ilow pattern and protected área

2,1.4 Protected objeets

Biologíeal safety cabinets are designed to protect the operator, íhe laboratory environment and work
materials from exposure to infectious aerosols and splashes tíiat may be generated when
 :>...inulsb.-!•■ rjiPt^rÍBÍs contpining infecíious r,'",","">'" v\rh $$ nrimarv cultures, stocks and diasnostic
speuimciii.
2.1.5 Tcclmical paramcters
~""~"-^--Jiáodel 11231 BBC 86
Parameters ^~^—-^
Extemal Size(W*D*H) 700x650*1875 (mm)
Intemal Size(W*D*H) 600x500x540 (mm)
Power Supply AC 220V±10% D 110 V± 10% ü
Frequency 50 Hz D 60Hz □
Consumption 1000W
Total Airflow Volume 230 m3/h
UV Lamp Consumption 15W

LED fluorescent lamp 8Wx2

Consumption
Downflow Velocity 0.33±0.025m/s
Inílou' Velocity 0.53±0.025m/s
HEPA Fílter 99.995% efficiency at 0.3um. Filter life indicator
Noise <67dB(A) j

Notes:
(1) Electric consumption power including power which operation área needs to load (Loading no
more than 500W)
(2) Base stand height 690 mm.
(3) Our company has right for changing the products, if we need to change and re-design, picase
forgíves us for not notifying you.
2.1.6 Performance Index .!
a. Biological safeíy functions
Personnel protection, microbial cdlony count <5CFU.
Sample protection, microbial colony count <5CFU.
Cross contamination protection, microbial colony count <2CFU.
b. Lcak-proof Cabinet
If cabinet pressurized to 500Pa, the pressure should be no less than 450 Pa after 30 min.
c. Integrity of HEPA Filter
Sean and detect the HEPA filter, the leakage rate at any point should not be >0.01%.
d. Vibra fio n ampíffude
i nú uct \ iüiüUuü aiiipiuuuc üeivv'tieii irequeucy ioi~í'¿ anu iiíkrtz i>¡ no inore inan 3j.un(niisj.

The average Ülumination is no less thaii 650 lux, actual iJIumínation is no less that 430 lux.
f. Mechunícal performance
StructLtre desígn is reasonable, high quaiity materials are adopied for íhe cabinet.
It can resísi sbape globa! deformatiem caused by externa! forcé.
The working surface wilí not oceur permanent deforraation when wejghl put reachmg 23kg.
g. Eléctrica! properties
The volíage mercases ío J 390V(AC) in 5s and keep for another 5s without breakdown.
Groundíng resistance <0.]£2

2.2 Froduct structure

2.2.1 Struclural composition of 1123! BBC 86

 '. Fi!'",f' 7. T ¡V lamp
l. .Power SOCKCL ¿. , .„./„ .......},.- .
3. Tube motor 9. Waterproof socket
4. Control panel 10. Work surface
5. Power lock 11. Base stand
6. LED Lamp .12. Footmaster caster
2,2.2 Structure introduction
a. Driving System of Front Window
Driving system consists of tube motor, front window, hanling sash and position switch.
b. Air Filtration System
Air Filtration System ís the mosf importan! system of BIOLOGICAL SAFETY CABINET. It
consists of blower, supply fiJter and exhaust filter. The ñmetion of Air Filtration System is
transferring filtered air to work área, ensure the down flow vclocity. and keep Class 100 cleanness of
work área.
c. UVUght
U V lamp is inside work área. So UV lamp can well sterilize all space of work área.
d. LEDLight
The Biológica! Safety Cabinet is equipped with LEü lamp. It can malte sure average illumination
inside work arca whichmeets standard requirements.
e. Air pipe
Air pipe is the ventage of differential pressurc sensor.

The air pipe should not be blocked and picase do not hang anytliing on the pipes,
otlierwisf it vviü effect wind speed and pressurc.
f. Power lock
When the power cord is connected to main power, svvitch the key for power lock, then the equipment
 Waterproof Sockets are locaíed on the right side of thc work área, whídi can be conirolled by
SOCKET bul ton.

(1) Please make sure the fotal load of sockets should be < 500W.
(2) The waterproof socket can only be waterproof wh en its front cover is lowered, and the
socket can not be regarded as a waterproof socket when the front cover is opened.
h. Fu se protector:
The equipment is equipped with mairi power fuse, waterproof socket fuse and fan fuse. Thcy are
locaíed near the power cord's outlet Fuse label is corresponding to the relevant speeifications. Please
refer to 3.2.
i. LCDDisplay (Liquid Crystal Display)
LCD Crystal Display is placed i ti the raiddle of the two pieees of parallel glass liquid crystal, there
are raany veilical and horizontal fine wire between two pieees of glass, Gontro! rods crystal
moiecules change direction through electricity or not, and wiíl have a picture light refraction . Low
power consumption, no electroraagnetic radiation, and the service life can be up to 1OÓ0O0 hours.

Picture 14 Large LCD display mdicates detailed key

parameters, ít is real-time display to refiect the equipment workíng condition, such as effective
working state of the fílter, vvhich is more intuitive. (Please refer to2.3)
j. Control oí'Front Window
Front window is motorized. It couíd be controllcd by control pane!. Glass door control motor for a
dedicaíed reversible speed motor, the motor has a large starting torqne, smooth rotation and so on.
k. Strucíure
~i) Biologieal Safety Cabinefs both sides and back área are negative pressure air channel.
Make a double isolation by air eurtain and cabinet between working arca and externa!
environment, and the working área surrounded by negative pressure ío avoid any leakage. 2)
Cabinet body is built of 1.2mm cold-rolled steel with auti-powder coating. Slrong and
steady,
 3) Work área ís tiiJiy made oí JÜ4 siamless steei witicn looks beautitiii anuwim corrosión

4) Base stand is made of cold-rolled stee! wiíh aníi-powder coating.

5) Soft íouch typc control panel, easy lo handlc and heautíful appearance.

2.3 Control panel

Picture 15
I.UVLamp 2. LED Lamp
3.Fan 4.Socket
5.Glass Window Up ó.Glass WindowDown
7.Mute 8.Power
9-Alai-m Status lO.Mutc Status
11 .Filter Changing Status 12.Temperature
i3.System Working Time 14-Humidity
lS.Glass Window Down Status ló.Glass Window Up Status
1S.UV Status 19.Socket Status
20.LED Lamp Status 21.Fan Status
22.Exhaust Filter Differential Pressure 23 Jnflow veiocity
24.Supp¡y Filter Differential Pressure 25, üown-flow veiocity
 ió.Filter Working Time 27.UV Lamp WorkirüiTime
-lo.Kescivauoii njiuiíg
a. LCD Screen
The working status of the equipmenl and operation can be seen on the LCD screen.
b. Softtouch button.
Biológica! Safety Cabinet's main functions conld be exeeuted by touch-buttons. User can opérate the
Biológica! Safety Cabinet eithcr by pressing the buttons on control pane! or using the remote control.
There are totally 8 common button on control panel.
TiTl
----■—': The power button, the main switch lo control other functional keys

- ■. - J: To control LED Iamp

—L!_—!: To control UV lamp. (ít works oniy after front window, LED lamp and hlower fan
are fully dosed.)

-~".J; To control blower working status. (It will not work when front window is fully
elosed.)

..~-J; To control socket power status.

1
-----•: Press MUTE button to stop volee prorapt

L. ,); Press UP button, glass window will raise.

I------>: Press Down button, the glass window will fal! down, and one key disinfection
operation can be carried ouí when press this key in the resting state until the screen lights up, then
the UV lamp will turn on automaíically añer IOS. There are totally 8 common button on control
panel.
c. Clock Adjustment:
Turn tlie power key, so machine is ín standby state.
Press the líght button, and then press the power button for 5 seconds. Then you see the state of dock
adjustment after a buzzer alarm.
Firsíly, minute position is flashing, press UP and DOWN to aüjust to present time. Then press the
MUTE button switching to hour posiíion and adjust lo present time. After that, press the líght button
fírst, and press the power button for about 5 seconds. Data wÜI be saved after a buzzer alarm.

2.4 Remote Control &Foot switch

It is inconvenient for the users to opérate from a distance. Small & líght remote control is flexibly to
 he used to control al! the Jiinctions of íhe cabinet in a disiance <íim. 3u,J rango, i ¡le opuaio¡ vuu

This remote control adopt speeific chíp which is featured with good antí-jamming performance,
longcr control distante and high control precisión.

Buttons of Remote Control: 1. Power (POWER) % Reservation Time (SUB)

3. Timcr (ÍNSTALL TÜVIER)
4. Confina (CONFIRM)
5. Cancel (CANCEL)
6. Tumup (+)
7. Tum down (-)
8. Fan (FAN)
9. UV (ÜV)
lO.lUumínation(LJGHT) 1J. Socket (SOCKET) 12.Mutc(MUTE)
13. Front window up (UP)
14. Front window down (DOWN)
a. Reservation Time (SUB)
1) Connect power, open powcr lock, and prcss tbe reservation timing button (SUB).
2) Adjust the time (minutes) by "+" or "-" button, Press the confírmation butlon (CONFIRTvl)
to confírm. and then adjust other minutes and hours position data in the same way.
 3) After íhe lime is conñrmed, the corresponding display lamp lights by setecting the funcíion
buttons (such as UV).
4) Press the POWER button again, the reservation function starts. Reserved time starís connt
down. The corresponding setting function starts when the time counts down to zero.
b. Timer (INSTALL TIMER)
1) Comiect powcr, open power íock, press button (POWER), the corresponding display lamp
lights by seiecting the function buttons (such as UV).
2) Press button (INSTALL TÍMER), adjust the time (minutes) by "+" or "-" button. Press
button (CONFÍ.RM) to confírm. and then adjust other minutes and hours position data in the
same way.
3) After the time is confirmed, the Timer function starts. When the time counts down to zero,
all the functions \vill be off, the cabinet will be in standby mode.
c. Application of Reservation Time
Biological safety cabinet is equippcd with special UV lamp. When tuming on or tuming off íhe
 vtiuiíiuL, siujiiAítuuu tiuic ui o V ¡cüíip &ÜÜLUU ue tu icasi JU minutes. In arder tu save íhe watüng time

automatic turning on or rurning ofí the cabinet after íhe sterilization fmíshed. Kcscrvation time
setting rangc is from 0 to 99 hours and 59 minutes. This function helps operators tu save time and
improve effidency.

2.5 Instructíons for Operation

2.5.1 Norma) Operation No tice

a. Make sure input voítage is correct and stable. The rated load of mam power socket should be
higher than cabinet consumption. Plug musí be well grounded.
b. ín order lo avoid air turbulence, the operator should slightly move his arms during experiment.
Hands should stay inside íhe working área at íeast 1 minute before operating. In order to
decreasc the times of antis moving into and out of the working área, prepare all the necessary
ítems inside the cabinet befare starting experiment.
c. Moving principies of different sampíes inside cabinet: When tvvo or more samples need to be
moved, be sure thaí fow-pollutmg samples move to high-polluting samples. Movement of iteras
should also follow íhe principies of slow-moving.
d. Samples placed in paraílel: Samples should be placed in the cabinet paralíel to avoid
cross-contamination between samples and blocking back air grille.
e. ín order to avoid samples being sucked into the negative passage or the blower, do not place son
and slight samples (for cxample: son tíssue) on the surface during experiment.
f. The weight of items placed in the cabinet should be no more than 23Kg/25*25crrr.
g. Avoid vibration: avoid using vibration cquipment (eg centrifuges, vortex oscillator, etc.) inside
the cabinet. Vibration would cause Iower cleanliness of operating área and affect operator
protection.
h. No fíame: No fíame is allowed inside the cabinet. Using of fire will lead to airflow disorder, and
fílter damage. If sterilization is required during the experiment, infrared sterilizer is highly
recommended. i. HEPA filter life; With the usage time inereasing, dust and bacteria accumulate
inside HEPA filter.
Filter Resistance is getting bigger, when ir reaches the máximum point, there will be audible and
visual alarm. Please rcplace new HEPA filter, ofherwise it will affect the safety performance of
the equipment. The used filter should be processed as medical waste. j. There is a negative
passage surrounding the vvork arca, which is sealed strictly in the factor}'.
The operator is not allowed to remove or loóse screws of those parts. If necessary, pícase contact
service personal. k. Front Grille is used for air intake and drain. Do not block it otherwise it will
affect airflow.
Armrest is recommended to soive this problem and reducing the operator's wrist fatigue. 1.
Long-term use of biológica! safety cabinets will inevitably cause pollution (e.g. HEPA fíiters,
comer cabinets. etc.). Ln arder to steriUze thoroughly every 500 hours, formalin (formaldchyde)
fumigation sterilizer is recommended. After sterilization, neutraiize forrnaldehyde gas with
 ..:.,:;,o;:;..-. l.j-1^.^,-. ^Í^-ÍWH.-, MaKe sure no steniízation gas escapes duriiií! the whn'f

m. Íhe máximum siorage period is une year. íf Ihc period is more than une year, performance fes:
should be done .

A
£-^-i Senous dcclaration: wc wíll íake no responsibility for rísks caused by improper
openttion and man-rnade damages!
2.5.2 Operation Process
a. Connect the same power reply, as required of equipment
b. Open íhe power loek, LCD display ligliís up and alarm rings at the same time, tiien the machine
enters to standby status. Waiting for the operator to input button to use it.
c. Press POWER button, then the followíng fbnctions are available: LED lamp. UV lamp, Fan,
Mute, Sockels. Front window up and down, Reservaíion timing

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¿~^-i When front window is open or oíher button is pressed, the UV lamp can not be work.
d. Before doing experiment, please sterilize the cabinet for more than 30 minutes by UV Samp( the
front window must be ctosed)

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¿~¿-l(I) For safety of eyes and sldn, people should ieave room during the UV sterilization.
(2) UV lamp intensity should be tested regularly. If there is no test conditions, it should be
replaced, recommend once for threc monrh.
e. Picase move the front window at 200mm heiglit from the work table, turn on the fan, make sure
the experiment should be started after fan working for at least half an honr.

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c-L-i For operating safety, please put testing materials inside the cabinet ín advance, and keep
the front window at 200mm height from the work table during operation.
After finishing the experiment, please move the front window down to the bottom, and make sure to
sterilize the cabinet by UV lamp for 30 minutes before turníng off the cabinet.

2.6 Daily maintenance

Because the operating lime will directíy affect the judgment of maintenance needs, we recommend
the user keep a detailed record of operating time for reference.

t_í_j\Vhen doing maintenance, please pay attentíon to cut off the power, so as to avoid electric
shock
2.6.1 Preparations before maintenance
Soap, hot water or wann water, a soií cotton cloth, dry cioth or towel, medical alcohol or other
disinfectants, 100 dilution ofhousehold bíeach, abrasive household cleaners, sterile water
2.6.2 Cica» the cabinet suri'ace
¿i. Cicau tiic opuiíiúiig ai cu &ui luce
Wipe the entíre surface with a soft cotton cloth or towel soakcd with concentrated liquid soap, then
wipe up tlie soap with another cotton cloth or towel soaked with clean hot or warm water, and then
wipe the surface with a dry cotton cloth or towel rapidly.
I7or the contaminated or dirty work surface or surnp,, use 70% medica! alcohol or other disinfcctant
to wipe.
A
¿-l-iDisinfectants uscd for wiping should not damagc 304 stainless steel.
b. Clean the externa! surface and froní window.
Use son cotton cloth or towel to wipe the surface with non-abrasive household cleanser.
2.6.3 Overall maintenance períod
We suggest comprehensive maintenance períod is one- year or 1000 working hours.
2.6.4 Maintenance methods
a. Daily or weekly maintenance
1) Disinfect and clean operatjng área.
2) Clean the externa] surface and froní window aronnd the operating área.
3) Check the various functions of equipment. 4}
Record this maintenance J'esult
b. Monthly maintenance
1) Clean the the externa! surface and front window.
2) Wipe the working table, inner wall siírface of operating área (exctuding the wind
distributing grid of operating área) and the inner surface of glass door with 70 % medical
alcohol or household bíeach díluted 1:100 (í.e, 0.05% sodium hypochloriíe). Then wipe
agaín with sterile walcr in order lo elimínate the rest chlorine.
3) Check the various functions of equipment.
4) Record this maintenance result.
c. Animal maintenance
1) Check the two conveyor belts of front window drive unit, and ensure tbat their tightness is
coincident.
2) Check the UV lamp and LED lamps.
3) Apply for testing the overa!! performance of cabinet on an annual basis to ensure the
performance safety. User is responsible for testing costs.
4) Record this maintenance result.
2.6.5 Storage conditions
Safety cabinet should be stored in a relative humidíty no more than 75%, the temperaínre is belovv
40DC, in the warehouse with good ventilation performance, no acid, no alkali and no other corrosive
gases, storage períod shall not cxceed one year, safety cabinet for more than a year needs to unpacked
and checked. Only the tested and qualifíed safety cabinet can be sold.
'7.7 iVi'HhíuIs ;:rfu'? p'.'íu■■'■.:^í'uv<''•:^ n - r JPJnfí'dV- ■?

Details in tlie After-sale service manual

Disinfection is necessary when any contamínated part of the faiosafety cabinet needed for routin
raainrenance, replaccment filiéis, and performance testing, etc. Before doing eertifícatión test an gas
sterilization, all interna! working surface and the exposed otiter surface should be disinfecíe wiíh a
suitable disinfectant. what is more, using Ciass II biosafety level designated medicament t steriiize
the cabinet by gases is required. Ciass III biosafety level designated medicament i recomiaended
after the use of the cabinet. The cabinet, imder the risk of being polluted by th biological factor,
shouíd be sterilized before sliding posición.
The polluted working smface caused by overfiowed and spilled reagents also shouíd be sterilized
Mosi of íhe cases that need gas sterilization use dcpolymerízed triformol as the disinfector as notec
beiow, the period parameters and its validity of each model and size of the cabinet are required to bs
lísted before change sterilization method. The relevance of the materia] has connections vvith the
absorption and degeneraron of spare decontaminan!, whích is the key factor of keeping the cabinei
integrated and the sterilization time. There need those alternaíive sterilization way in some case, sucli
as: slow dísease virus. The sterilization method is used agaínst tiie consult beíween the end user and
certiíícatión authority. Pointing out the given arca, gas mask, safeguard procedures, eorresponding
test, medical monitoring, conveying danger and íraining, record and reserve etc. and follow these
steps.

Before sterilization, all the hydrogen chloride should be moved away from the
cabinet. There will result in carcinogenic substance-BCME when it mcet with formaldehyde
under the ambient air.
a. Figure out the total volume by multipíying the height, width and depth.
b. The required weight of the triformol will be worked out through the total volume multíply
1 Ig/itf, fíguring out the weight of ammonium bicarbonate or its alternative by cheraomeíry.
c. It musí be bubble-tight if there are exhaust pipe with íhe biosafety cabinet, which can be
reaiized at the end of the pipe or sealíng ai íhe control valve if there is valve nearby the
cabinet, it need more triformol to compénsate íhe added volume if the length of exhaust
pipe is more than 3 meters. If the exhaust recirculation of the cabinet connect with the
exhaust system of the constructíon, interrupting the connection system of the biosafety
cabinet with constructíon and sealíng (by using plástic film and plástic sírips)
d. Seaüng by plástic film as the vvaste gas is exhausted into the room from biosafety cabinet.
e. In order to urgently elimínate formaldehyde, steriiize and elimínate formaldehyde after
neutralizing, puttíng a tube nearby the biosafety cabinet in advance whích is required to
connect with chemical smoke hood or other exhaust devices that is suitable for releasing the
hannful gas.
f. Pul the heater, such as electric-heating frying pan whích can be bought from market,
fonnaldehyde genera tor or neuíralizer on íhe working table. Sct up the temperaíure al
232°C'--2460C, íhe triformol is spraying over the surface of the heater devices equably.
g. The neutralizer with the heating devíce is placed on íhe workbench. Neufralizers
(Ammonium bicarbonato or equivalent) should be isolaíed from the air Ín the cabinet before use.
The following lwo examples Ühistrate how to achieve air separation. Eg.l: Ammonium
bicarbonate or substiíute is uniformly sprayed on thehcated surface ofthe heating device and
covered with aluniinum foil to prevent the carbonic acid hydrogei] ammonia or its substiíute in
disiníectíon reaction with formaídehyde. The aluminum foil should be placed so that ammonia
can be released during heating or ready ío remove aluminum foil at the beginning of !he
neutralization phase. Removal of aluminum foil is not allowcd wlien tbe formaídehyde leakage
out ofthe safety cabinet of. Eg.2: The safety cabinet with the gloves for a whole plástic film
seaiing. Ammonium bicarbonate or equivalent subsíitutes are placed in a container ín a safety
cabinet. During the neutralization phase, the dishifected personnel into the safety cabinet through
the gloves do not break the sealíng system. The ammonium bicarbonate or equivalent subsíítuíes
are removed from the sealed container and uniformly sprinkled on the heating surface of the
heating device, then the heating device is energized, the ammonium bicarbonate or the substiíute
is heated to reléase the ammonia. h. Puíting heating píate, water beaker and hygrothennograph
on the work table, do not connect
the wíres to the power supply in the cabinet. i. Seaiing the front operating window with thick
plástic film and plástic strips. Seal all the área where possible to leak. Such as wire outlet, the
surrounding of front operating window and joint between plástic film and biosafety cabinet. j.
Measure the temperature and humidity inner the cabinet. k, The humidity is at 60 %~- 85 %
when temperature is above 21 °C, heating the water in the
beak up to the expected temperature and humidity. 1. Before depolymerizing the
formaídehyde, strictly restrict entty to the área around biosafety
cabinet orroom in accordance with related regulation and safety measures. m.
Connect the wire ofthe heaíer with socket outside the cabinet.
n. 25% formaídehyde is depolymerized open the safety cabinet fanlOs-35s, after The para-
formaldehyde is depolymerized by 50%, 75% and 100%,repeating the above steps. lf íhe safety
cabinet fan does noí work. Use auxiliary fans to promote the air circuíation Ín the cabinet, or
prolong the disinfection time over Step P . o. Disconnect the power supply ofthe heating píate
and heater for the use of informo] p. Keep the biosafety cabinet isolation at least 6 hours, it is
betíer ío íeave it for a nighr. q. Prepare the neutralizen power off the heating devíce and the fan
of biosafety cabinet unti! ammonium bicarbonate wiped away, the operation of triformol is also
the same, after the decomposifion of 20% ammonium bicarbonate, open the fan for lOs-—15s,
using assísted fan or electric fan ío promole the air circuíation inner biosafety cabinet or prolong
the neutralization time for at least 6 h if the fan ofthe biosafety cabinet does not work. r. Keep
the biosafety cabinet isolation at least 1 bour before open the seaiing membrana.
BIOBAS€

s. [f exhausting the fomia)dehvd¡? cfi'iyed Hy ntíi!transí ion vh 'ube, tear om the plástic COVÍ

biosafety cabinet will be suck in and cut one or two openings make the fresh air be inte ti'
cabinel if the tube work well, then íhe formaldehyde is exhausted via tube.

2.8 Replacement parts list

J 3 23 i BBC 86 replacement parts list

Number. Ñame Specification

AC01 Fuse 10A

AC02 Fuse 5A
AC03 LampholderTS LG13-01A
AC04 UV Lamp T8 15 W
AC05 LED Lamp 8W
AC06 UV lamp baüast 1*TL8-18W
AC07 Upperfílter (Exhaust filter) 570*380*69mm
AC8 Lower fílter (Supply Filter) 570*460*69mm
AC9 Fan DH146
AC10 Control panel LCD control board (strong circuit board,
weak circuit board, display screen)
ACU Remote control (with battery)
AC12 Key seíection button YJ139(LA38, LA39)
ACÍ3 Glass 6I9*590*5mm
 BIOBASG

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3.1 Common fauits & solution

3.1.1 Warning and reminder

Digital display of pressure difference, digital velocity display, audible and visual alarm system.
a. Over safety height alarm for front window
There will be audio and visual alarm when front window is lifting over safely height. Same time
LCD display will twinkle exclamation mark. Then just adjust the height of thc front window.(Front
window height setting valué is 200min).
b. HEPA fílter pressure difference alann
There will be audio and visual alarm if pressure of air supply fílter or exhaust fílter can'i meet present
valué, at the same time LCD display will twinkle exclamation mark. Remind the operaíor lo replace
the fílter immediately lo protect the operator's safety.
c. Velocity fluctuation alarm
There will be audio and visual alann if the inflow velocity and downflow velocity below 20% of the
standard valué, namely, inflow velocity below 0.42m/s, down flow velocity below 0.26m/s, at the
same iime LCD display will twinkle exclamation mark to remind the operaíor pay aítention.
3.1.2 Trouble shooting
Picase confirm whether the power is connected or not, whether the power cord is obvious damaged
or not, whether the ñise is good or noí, and whether the power locks are in the open state or not
before the fault diagnosis.
Fauits Check parts Measures - -.. .■; •..
LEDlamp doesn't. Circuit Check circuit.
LED iamp Change it.
Control panel Change it.
UVIamp doesn't Front window, fluorescent Check the front window, LSD lamp and the
work. lamp and blower blower is open ornot.
Lamp holder Tube and lamp holder is connected securely.
Circuit Check circuit.
UV lamp Change ít.
Micro Switch Check if Micro Switch is broken.
Control panel Change it.
Button doesn't Control panel Make sure the power conneets well and the fuse is
work. well.
Check if the button is broken.
Make sure the connectíng wire is connected well
Change control panel.

27 II
 BiOBASG

| Faults ! Check pmts TMCASMÍ'-T

Blower doesn't Front window t-roni. window is open or not, blower works c
work. when the front window is open.
Micro Switch Check if Micro Switch is brokcn or works fir
Blower If blower is broken, change it.
Circuit Check circuit.
Control panel Change it.
No elecíricity in Socket fuse Check if socket fuse is broken.
socket. Socket Check if socket is broken.
Circuit Check circuit.
Control panel Change it.
Pressure or air speed Gas circuit Check whether gas circuit has dropped, is
display ed broken, or jammed.
incoirectiy. Control panel Change it.
Fronl window Circuit Check circuit.
doesn't work. Motor of froní window Check front window motor.
Transmissionpart Check transmission connection and leod rail.
Control panel Change it
Remote control Remote control Check if the Remote control is broken or not, i
doesn't work. if there's electric in the batrery.
Connection cable Check whether main control panel and display
board is connected well.
Control panel Change it.
No elecíricity in Power supply Check power supply conneets well.
equipment. Power wire Check whether power wire has obvíous damag
Fuse Check if the fuse is good.
Power key Check if power key is open, is broken or not.
Transformer Check whether the transformer works normally
Control panel Change it.
Dísplay doesn't Connection winding Connection winding displacement.
work. dísplacement
Display screen Check that the display screen is irj good co
dition.

Control panel Change it,

No alann. Micro switch Check whether the micro switcli is good, and ií
works normally or not.
Circuit Check whether connection circuit of micro
switch is good.
Control panel Change it.

IB2S
 BÍOBASG

(1) The above eléctrica! parts niust be operaled by a qualiñed electrícian in safeíy eonditions
(cutíing off power suppíy). The other parts are not aüowed to remove. otherwise the user
should take responsibühy by them.
(2) When faihires are not occur, and the operator can't solve, please notify our maintenance
department immediately. For your safety, please do not maintain equípment by yourself.
(3) The maintenance of this equipment is undcrtaken by trained and recognized technicians.
(4) If you need to order parts, contact the agent or our technical service department, and please
indícate the model and serial number of the cabinet purchased.
3.1.3 Simple accessories replacement
a, Repiace the tuse
Waleiproof socket are located in the top operation panel. When repiace them, turn off the power and
disconnect plug, use a Phillips screwdriver counterclockwise pressing screwed fuse holder, remove
the ñise out and repiace a new fuse, and then clockwise pressing screwed ñise holder. Fire wire fuse
is located in the side of the cabinet operation panel, take out of the fuse holder using a slotted
screwdriver and repiace with a new ñise, and then press it back.

PowerPlug Fuse

Picture 19
b. Repiace fluorescenr light
When replacing lights, make sure that the power is off, open the operation panel like shown in
picture 20, use the control panel support frame (ñxed in the inside position of the control panel as
shown) to open the gray operation pane), then üke picture 21 shown removing one lamp tripod and
then take off the lamp, install the eorrespondence type of lamp, inserí the lamp tripod (make sure the
lamp tripod is fírm).

29 I!
 BIOBASe

Using support bar which

is at the left ■ and i'íght
sides oT the operation
pane] to prop up the
operation panel

Use a Phillips I screwdriver to rensove fasteníng screvre which is

at the left and right sides of the front panel j

l^'.n'.'t.; ' »IK''Í fui» hwi»

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i Picture 20
iI!

Picture 21

c. Replace the UV lamp

UV lamp shonld be replaced regularly according to the frequency of use, when using UV lamp reach
to the time of 600 hours, we recommend to replace the lamp. When replacing, fírst make suri the
power is off, and then screw the bulb 90° and take it off, then take the correspondence type o lamp,
and put it to the lamp holder and and screw 90° in reverse direction. Afíer replacing the U\ lamp, it
needs to keep pressing the button of UV for about fíve seconds when the machine stayí standby.

1130
 BIOBASG

V
UV Lamp

Picture 22

3.2 Label Descnption

Label Descnption

Biological hazard label

F10AL250V 1OA power ñise labe!

¡Tubular Fuse Forl

[Socket Operating área 5A socket ñise labels
IF5AL250V :

Ground label

Glass door super eievation warnmg label

UV lamp alarra label

GI
 BIOBA5€

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Mniliiíimi po»cr 5WV

Plrxf ■« tlttTfJt'Futíate *J:1 tiwJ tnthljf £.

Load requirements label

Biológica! infection risk

Caution label

fílter upstream FÜterupstream

Waming labels of íhe front window

Sewage valve labe!, sewage valve biohazard Íabel, warning label

18 32
 B10BAS€

Life time of biosafeíy Cabineí is 8 years írom production date on Ihe Jabel.The warranty period of the
purchased product is subject to the sales coníract, accessories are free within ihe warranty. Supplies
are not warranted for consumable.
The following conditions will not be covered by íhe warranty,
® The serial nuniber of ihe equipmení provided by the customer is incorrecl.
® Malfunctions and damages caused by violation of the usage, precautions and intended use
described in íhis user manua!.
® Faílure and damage caused by operations such as inspección professionals, doctors or
laboratory personnel írained by our company or llie agent designated by our company.
@ Faüureand damage caused by repair ormodifícationof the other company.
® Faikire and damage caused by use with equiprnent other than those specífíed by our
company.
@ Faiíure and damage caused by the inconsisteney beíween the operating environment and
íhe operaüng environment (power supply conditions, instalJation environment, etc.)
specified by our company.
® Failure and damage caused by irresistible natural disasters.
® Failure and damage caused by our company's uniníentional movement or transfer
(transport) after the equiprnent beíng ínslalled.
© íf íhe equiprnent fails due to the use of consumabies such as reagents noí approved by our
company, it is not within íhe scope of our company providing maintenance services.
© Other faüures caused by non-producís themselvcs.
© During íhe warranty period, if there is a failure caused by defeets in íhe design and
manufacture of our company, the repaír will be carried out without compensaron. Our
company will íake corresponding countermeasures according to the fanlt contení.
We can provide equipmení drawíngs and neccssary technical data for maintenance companies or
personnel trained by our company.
After íhe warranty period expires, our company can continué to provide fee-based repair services.

33 !S
 ~ i *■ --F» . I// *

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