Biology Practical NotesClass 12B
Biology Practical NotesClass 12B
Dated
PoLLEN GIERMINATTON
txeERIMENT N, o1
- f pollen qexmnation
Aln To study
Sllds.
Requement-SucHOSe l0 gm, Borc acrd,
lomacalcfum ntrate 3oma
sulpha'
Potassum
omq
tate loma ateYmaanesiim
Hibc us flowex
te dic\led
fo pollen qalas safanio glycoal, slde
Cove sip etc
Obsevatieo table.
laTotal no
EloueHS. of bNo.of pallen% of pollen
pollen qrafa choulaa getooaton
|Seen gemiaahon
Hibfscus Lo
Conclusion
a a
Pollen qafs qernnate Sugan Soluton
ohich act nutot fos enegy
<br>
Dated
Neaetoe ceL
Male
9rmete
Gieneete
Cell Tobe nucleus
<br>
Dated
each Side
Pollen dusted ast be dipped i a
the tiest
soluta Ca the cauity
la the sltde.
*Place he ŠLp canefuly
Check aßn bubles.
<br>
Dated
txpeRIMENT -NO- 02
DNA foom cuailable pant
Aloi- Tsolatn of
mateHal ohích Such asas spnach
papaya ,onfbn etc.
Plant mateHal,:tilled uoater
Requirement
handLOash salt, chilled ethdnol
petrds qlass tod.
Observatioa:-
ObsesNotûo- Nddiional of ethaol to the
SolutOn ccuses prlciptaton of
ONA
DNA fibnes get s poiled on glass od.
Pyecaution Wash he plant mnaeual
houchy with dístilled ualen to
4enoNe any dust pahtles.
ne eled fos ONA
The chemical sdlatoo of
should be manufactured bu standexeA
by
phamnceulícals
<br>
Seguance
helix of ONA.oUCleodes
nucleotde ea a Dated
olant oc Couble
Base pa ir
Adenie Thyninc
Cuga
Phosphate
bachbor Hunanne Cytoine
Teacher 's Sgnature
<br>
Dated
txeERIMENT No. 03
Alon i plat populatba densty
Study of plant
by quadrate method
Requtmet
- Meten Scale, stroq nails,
papes penciL, digqer, tape etc.
ObsexNahon
SNNoarne oNae of aduiduala plant ot
Specie s o
spec ial counted differeot
quondyates
Resu+
Densiti
of
It he nuneca
SAnength Speces pe unit
s
calculated
<br>
1 m
Nail
Y Thread
A
-Species
B
f-Species
Hte
Species C
1 m 1 m -Species D
-Species E
-Species F
Hte
1 m
Fig. A quadrat with sub-units
<br>
Dated
o
1)3 Populaton densiy ofa plant species the
guen field
oo. of a spectes
=Total iodiidual of
the quadates
o
f
No. quadrates
That io populaton density per squase metre)
T
Ci) avoid Abe UaHatibo intu
dataquadate
a
chould he selected
feld.
i) Oaly Seu plast speces oust be
be cnsdened fos
dta collectian ia one tne.
(e)Cout 4he no. of plats caue Sully
<br>
Dated.
Lxpcnent No. 04
Stud of qoup
Aooi- forequency plat
quad cn
by qiadrat
populatoa
method.
Requiiement : A
paqes, lnete scale ,sriag
cwak book baa fa
5om
plant colleioo etc.
Obseruatioa
No. of
No SoecieS qucdaot Rodkuidual b
1 m -Nail
Thread
A
-Species
-Species B
-Species C
1 m 1 m - Species
Species
D
E
Y -Species F
1 m
Fig. A quadrat with sub-units
<br>
Dated
Poplatiaa fucauency ?
H s the
t fs
calculate d
falloo.
a
Populaton densisty
>
of quodants
No. oheh
Specis ocuH
Tota no of quodrants stadis
Puecautoa
,
To auofd the vanatôn
vaniaton to dota
a
quadses
should be Selected cothñ Creld.
Saly ew ploants spec must be consîdee
data collectoo Ra one tone.
Count te no of plants canefully
<br>
Dated..
txeERTMENT No. 05
Ain:To ptepane termpay mount of
ono
Hoot tip study mitoss
Requiremet Oaîon bulb, Conical flask
petrîdfsbes scissors, forceps ,needle
actdic acid HCQ, acetacaHminedisttlled HaO
sprit lampmicroscope, slicks ,couDislrp etc.
- a pouwer of
Obseuuathoo Uade lauw actangula
cell with pute nucleDUS aHe Sun
sructurel. Undes the hiah poueH of microscope
the follouofag staqes become dstact
–
Iottnphase Tt diuidig phase of
the cell cycle bluw tu
tuwo SUcces&e cell
diusoa evelops
Nucleas and nucleoUs aHe distiact.
Chomotia fbes pper formoa netuwork wftb
the nucleus and axe not distinquished oto
chronDsone
PAophase- Chromata materíal ehautins and
Condenses into thuead like stuctue
Called chHomosome.
Cach chYDnosone consist of tuo chomatid
iato at a
poiat called centronere
<br>
Dated.
Dated.
te
Mhe Root tips should be uashed throughly af
boilaa oith dilte HCe oteHsSe celldoo't
get stalnd.
The cld should be woarmed qety
slid much cboue
48,e flane of spfret lamp.
he HDot tip should not dy up duslaa cuamina
Land stenig
These should be no. ar bubhle a
the slide
<br>
ue)
nd
-Nuclear membrane
rith -Chromatin fibres
-Nucleolus
wer, -Cell membrane
i
be
-Cell wall
Interphase stage
osis:
Cell wall
-Nucdear membrane
fcell -Nucleolus
Nuclear membrane
Disappearing
Nucleolus
Chromosomes Chromosomes
long
Cell wall
basic Early prophase
Late prophase
cially
-Spindle fibres
ergy Separated
chromatid
which >Chromatids
-
for Spindle fibres
Early anaphase
pear Metaphase stage
like,
-Cell wal Daughter cell
-ChromoSomes Daughter nucleus
(Chromatids)
work
Cellplate
bres -Spindle fibres -Nuclear
to be membrane
d
like Late anaphase Telophassestage
mes.
Fig. Various stages of mitosis in onion root tip cells.
<br>
Dated
WTNO PouTNATTON
WHEAT ANo AcacTA
(A) HEAT
Comonents Wheat floueY ae Compsed
of spike lets uhich a
made- up
of bact Like structue glunes.
Dated
Stanen
Teacher's Sgnature
<br>
Dated.
AODPED TO PoLINATION BY
BTRs
(e) ELOER
Alloatfon is the pnocess of tanstomna poles
the Semale
o
rom he nale cOher of flouer
Stigma of the same oY di Sexeot louer.
Slouex bu bftd cal\ed orithdogy.
>
The flouoes bds oHe ctong
pollaated by and
aHe to allouhe brds to stau
adopled
the louer usithout ther oñgs geting
enlongated io them
The flouwe aHo tubleH anc the faciRhs noctor
suckíog by bds.
e flouet
e
odeless and brakt colanod tha
attract the brds.
While sUckfoa te ector the pollen qrah esqet
depasited on thney beaks and nck and is
trans for: to he pla t ey usit noxt
Teacher's Signature
<br>
Dated.
LNsecTS PouTNATTON
(c) BHaSsica s
Cocmneots:- This floueY braht
yellouw colou to attract
the nsects.
tuoo oute
Stamens aue delCesuent together
nner bg
Le,
Smallex and foH
Ouclen'es whh aue
beHe aHe fouH proriiaant
fou
Sought by bees and Posects.
Petuaia aHe
Comononte- he flowe Cornplete
Coloued Coollas.
showiog anc Rave variousl
Dated
Petal
Sttarna
SAylc
Stomen)
Ovany
Se pal
Cedce
Thalamus
Petal
Anthex
Style
Flament
Ovany
Sepal
ed cel
Thalamus
Teacher 's Sgnature
<br>
Dated
Spot2
Ai Tochserve lstudy pollen agran on stigma
shigono
throgh a
pexnanent sLide
Maliol equed ícrOScope, permanont
slide, foeshy packed seasonal
loueHS ,beaker etc
e
RHocedutei-The peMaant slide undea a
microscope and the pollen qexmination
fs obseved.
heay-Pollíaatba sekes to the transfer of
pollen qca from ante to the shiama
meanes
though biotfe at abote the complete
pocess îs as giuen belou.
Once the pollen grains aHe deposited on the
stiama Tt Stantt to qermioate corth the
obsextton
A
of utriets and uatey.
Snall pollen tube s
produced through the
ctyle to ovay move
The tube Cell out of te.e pollen qrah
andpollen
thuouah one
o0e of te,
of
cerm poHes foms
tube.
The oeneahue cell also pasc Roto tAnd
to foTm tLs S00n
Soon
doide mad ee qamete
<br>
-Pollen tube
Male gametes
Style
Dated
Dated.
OvARY
s bounded by qexmínal
Comments- OuaHy
SRled cuith stoa.
epitbium and
follicles a
dieferent
Sona has gaflan Jhesee acludes.
stages of developenet
a) Goouofng folcles.
b) Rumay follfcles
Matune gaffaa Eollecles.
Sollicles
Ruptused araffaa
e) Copus luteun
Moatuseqora fan folleles cans fsts of central
cell.
OUuo SuoUnded bu follfcles lollclan
9copus Leum s
fommod altee the HeptuHe
of graffian Gollicles
<br>
ollele
Dated
antun -Matue
Garallron
foliele
Teyay
cotth
Covtex
albugt'noa
dauy
o99Stcon
foniea
ooqoniarinann
-fellicle
wteum
(apus
Blood
Teacher's Signature
KK
<br>
Dated.
IesTIs
Comnets Testis fs composed a lanqe numbex
semfeeUS tübules
of
s Liaed by qerminal
each semiareOUS tbules cell
lepithilLium ond contains seveHal layer of
Ro Sequence.
the folloofag
tolbule
Spexmatogonfa along the periphery of the
bRribnaty spermatocyles
9
Secondoy spermatocytes
7) Spermatds
Spermatozoa
c
Spermatid
d -Secondary
e spermatocyte
? Primnary
spermatocyte
d
s, Sertoli cell
ne
-m
SS
Spermatogonium
Dated.
SpoT 4
Diplotene. miqrate
The tuio homoloanus chonnosome blus
apat and dstag des înteqrate the
chonosorne
ookinesis:at The condensaton of chromesome stop
thfs stages and chiasmata is cleasly
Nistble under an electeon miTOScope The nucleas
and the nucleas enuelap dsappeaM. at his
ctages and te CentoSome mouQS to te eauotor
<br>
Dated.
Leptotene Zygotene
Pachytene
Teacher's Signature
<br>
Dated
Dated
SpoT. - 05
T.S of Mamnalian Blastula
Aion i-To the
obsexve IS of mammalian
pexmaneot slides.
blastula thouah
Lobsenuatiani- Blastula appeat sphene
usith cauit knon
of
as
blastomeyes
blastocoel Oute layeH
as tHOphoblasts obsexVed one end
Kaouone
of blastuDa shous C
ceYullaH masS
to te. trophoblastThis
one cell mass
Blastocoel Micromeres
Trophoblast
(each cell is a blastomere)
-
Blastocoel
Macromeres mass
Inner cell
:
Fig. A:TS. of Blastula Fig.B Human Blastocyst
<br>
Duted
SpoT :06
Seartaat
To study mend eltan foher itance Clau of of
-oo) ustno seeds of difexent colau (Size
any plat
(AManohybd Cuoss
A
Joo perr a
Roocedune i- lot of about seeds CUHe
taken în an enametray.
The OUnd nd wuakled seeds aue sepanated
Lact aad put different petridíshes.
The Oumbex of the tound and uriokted seed
aHe and ther
ooted and apprormate oatto fs
calculated.
Ihe pocess is Hepeated for the othese Contmstiog
tait fhe
of ee. Yellow and cgeen Colout
Obsenvotaai
S. JchaHacteH /Trait Total o.of No. of Seed shoig AppDximae
No. of seed Confhrashha fom
seed observeAo Cat Hotio
(D Seed shape lRound 8olR): Q6lu)
0Hiokled)
(9)Seed Colounlyellou 3-01:L
<br>
Parents RR) X rr
Round seed Wrinkled seed
Gametes R
Fgeneration
Rr
All round seeds
Selfing of
Rr X Rr
F-generation
Gametes
Fa-generation RR)
Rr Rr r
Phenotypic ratio Round (dominant) Wrinkled (recessive)
3 1
Rr
Genotypic ratio 1 (pure round): 2 (hybrid
round): 1
(pure wrinkled)
ON
Dated
Obseruatoni
no of Seed No of yellow Wo.of Yellow
Total wsuakled Seed green green
No. observed Hound. Seed nnkle
Rauod seed see
51 145 l6
<br>
rryy Parents
RRYY
(Red flowers and (White lowers
yellowseeds) and green seeds)
RY ry Garnetes
RrYy
(Red flowers and F,-Generatio
yellow seeds)
On
selfing
RY RY
Gametes Garmetes
rY
RRYY
Ry Ry
RrrY RrY
RRyy F,-Generation
ry rYy
Rryy Rryy
rryy
<br>
Dated
Conclusiaa i
o
The Ctfo yellou nound ,yellauw irinkled.
urîokled
gHeen MOundgseen ornkled approxrmately
9.3 3:1 ohich is excatly the Same
Lobtaaed Mendal ohybrid
CsDSS.
Dated
SPOT. :- 07
Rotepaued pediatee chasts af any one ,
aenetic traits such as ollhg of tonqus blaod
goup, tans Lobes uiadoo's puls and Coloun
bleadness
A s a
wdauo's Peaki- pediasee uisual shouing
the pattera of heritaaces
fox trart.
Symbals aad Rules
O
Unaffecte fenale
d
Unaffected Men
Widous peak fs a
hai Lihe and
aad that foms
dizhact peak on autoSome
toked domiaeot traits
ToianSmissioo of traits occuH hon parrents affected.
eRhe Sex males and Sernale eqvall
The
to
pediguee
be present
s
a
vertical
Qach
i.e. the toait
f the qenerahons,
mankecl
Dated
Teacher's Signature
<br>
Dated
Dated
<br>
Dated
Blaod sunps - A
pediasee s aa
usual shouing
a
the poattero inhenrtoance fox taits.
Synbole aod Rules:
Vaaffected males Uaaffected Seonale
AStected males ACfeced emale
Likes pauects together oith oa Lae and then
Lonake weHtele Line to Connt to offspring
STohenitance of blood group fs exarmple of
dominonce multiple allelson and o-domioace
Gueen fox AGo blaod oqroup haurag 3
alleles
aMe domioance
ohile Hecessre.
amJrufs,yI
<br>
Duted
SPOT.- 08
Contolled Pollaation -Emasculaton, baggtog and
Lagglog
L. Conutational plant bHeedína roqram ovolve
bsinang under human Controll sHeproductiue
pocesses that lovel to seed and fruit Sosmatíon.
Q. FoH this Controlled pollioatans desiable using
males and Semale pasents hauina desired traits.
3. Oae of the process To EEHY Cover the emasCulated
CloueHS a
oith plastic to protet
undesred pol len s
bag
called
protet t fom
baggng
4. Just afte des iHed cross polltoat'on
Cloue agah CoueHed orth te
the pollinated
Then fos bag inmideatel
dentiaton ,lobellag
fs Called as Taggng
of
female patents
coith desisd
lower
chanacenthe procedure.
<br>
om nd e em
parent ed, asha
bagging.
emasculation
wrinkled seeds
to take polythene
the
bag and
Emasculation
immediately
put
appropriate
so
Plant
that
tags
selfpollination
A
Tagging
on
them.
Plant
B 1his
and
Bagging
is
called
undesired
tagging
cross
<br>
Dated
Eotamaeba:
Phylum RoozOoa
Class :Rizopause
Type :Cotamnocba Buystaly tca
s c
Extexoal Rahe It an
uncellubn oaonim
with nequh shape.
Cons ct feu a
food vacunes The
s
Cootrattle vOcuals absent.
Cysts oitk fouL oucler aHe present
pxe seot
I Constt nucles Located eccentrtaly
the cell
Diseae :-
Eotamoeba
a
hustalftca s an oraanm
found tfRe atesthneof buman hat
s Hesponsible or causha amoebic dySentey
Syooçtnmna
* Abdomíoal pain * Naus&a
Cevet
* Fatque Kwaten diaxas uhmucLs
3. PlaEoagian- Tt
Round
& aa
uofcellulax
Loithh he
endopaasite
cell ct
ts.e ased
ned blood
46e d
person.
The paMaste at the sianet
<br>
Pseudopodium
Protozoa
Fig. :Entarmoebahistolytica
Entamoeba
histolytica
VIVAVOCE
<br>
s.
-Pellicle
-Nucleus
-Cytoplasm
Dated.
postaste that a
healthy
She nTected Senale anaphiis b?es
peson and transmits the SpaHOZods by hto tte
pepheHal blocdvessels f human then causing
malana.
Sumptoosi Nomih
,aussa
Headache
ills
shaking chills rom moderates to sea
3. Rioguorm
Ringdom Fuogi
Class Deuteromycees
TUpe Trchnophuton esobum
Exteroal feahHe - Tht fungus Seed on the keotn
the hyphaee coaiy and can be Snooth
Cottoc
Hyphase that a4s not staed aHe yellouish
s
broLon ,Hadd -brouon while oColou
D&easei Rioqquom is a
Communtcable funa
ofecon of the 8kia.
Syeaptoms
Red
Scaly tey skin.
and aised patche.
Mhey Nedds at
aHe he pexfphen han at the
cente and foms nog Like appeanence.
<br>
Dated
ENS
Excretory pore
-Gonopore Mouth
Dorsal lip
Excretory pore
-Lateral ine
Male
Female
Lateral line
Penial seta
Tail
Anus
Fig. :Ascaris
<br>
Dated.
Classiltaton
Phylum Nenato da
CHenUS Ascars .
LunbxoîdeS
<br>
Dated
Sumbolfc assocatioo
legumnous plunts cuscuta on hosf,Lchens.
lSybole associatoo ea Hoot nocdules of lesumiaos
plant.
) Root nodules
plaat They aMe foxm du to dissoclatiaa
n
Commonly found h Leqummous
with artogen fxîog bacleia Rhzobum.
Rhizobiun fs Sac le ra
> Root oodsles Conbne ptak coloe hemogobfa
prgment.
>
It also Contan enzyme nttoqenase uhithn helpr
formahon oof ammona.
Q
Cscuta on host- Cuscuta ,
Commonly oelesred
as to Oodden plat
belongs to the omanbel
Conuolwulaceae family
a
It is paHashc plant, andhas no
chlorophyll
So canoot make
nake ts Oun food
It giouos on other plarts, ustng their nuhrients
hs goth and uweakenme the host ploaot
<br>
BNitrogen
Compounds
DPhotosynthesis Nitrogen (N,)
from air
products
Bacterium
Fig. : Symbioticrelationship in root nodule formation
<br>
Dated
Dated.
SpoT
ng in bats,
Dated
plant sawgainuill
s ave
The Leaues of te
been modifed ento thrown for protecton
Lohole Leaves maden
+endris for provîdhg Support clunbers
Q. Aoolagaus Acaans The structure of anímal
' and unde study anology
e, they peformns sinilar
oot shaue
functons
diflerent organisms do Sane
structuHe the basfs f ogan
Aoalogous Orgas
The struchuse depet aa
the wings of bat and
eosects oa
Case
The
frelimbs of bat,brds and urngs and
fosects aHe h
fore lyng by tthese animals
houeu they
from each otheH.
ae shaucurally uy diferent
Aoslogaus
The
and
structue
pea
depfet passilora Cslern tendi)
Cleaflet tendril) a Case
of plat.
"The passloa and laaves
modEed Ro tendi whi
Suppert the plant dutihg clitnbng wheeas tfay
do not snae any steucwe smlcoGes