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+2 Unit Test Biotechnology Principles and Processes

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34 views

+2 Unit Test Biotechnology Principles and Processes

Uploaded by

Jityaa N. N
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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SSM CENTRAL SCHOOL SENIOR SECONDARY

UNIT TEST
Biotechnology Principles and Processes

GRADE-XII MARKS:30
SUBJECT:BIOLOGY TIME:1H

SECTION-A 10x1=10M

1. A single strand of nucleic acid tagged with a radioactive molecule is


called
A. Plasmid
B. Probe
C. selectable marker
D. Vector

2. Which of the following is not a component of downstream processing?


A. Expression
B. Preservation
C. Purification
D. Separation

3. DNA fragments separated on an agarose gel can be visualized after


staining with ____
A. ethidium bromide
B. bromophenol blue
C. acetocarmine
D. aniline blue

4.Match column-I with column-II and choose the correct option.


Column-I Column-II
A. Eco RI I. Bacillus amyloliquefaciens
B. Bam HI II. Haemophilus influenza
C. Hind III III. Escherichia coli
D. pBR 322 IV. Artificial plasmid
A) A-III; B-I; C-II; D-IV
B) A-II; B-I; C-IV; D-III
C) A-IV; B-I; C-III; D-II
D) A-I; B-IV; C-II; D-III
5. A biologist intends to use a polymerase chain reaction to perform a
genetic task. The biologist probably is trying to:
A) discover new genes.
B) clone a gene.
C) cut DNA into many small fragments.
D) isolate DNA from a living cell.

6.The Taq polymerase enzyme is obtained from


A. Thermus aquaticus
B. Thiobacillus ferrooxidans
C. Bacillus subtilis
D. Pseudomonas subtilis

7. What are the features of a plasmid being used as a cloning vector?

8. What are competent cells? What does the word “competent” refer to?

9. Identify the recognition sites in the given sequences at which E.coli will
be cut and make sticky ends.
5´-GAATTC-3´
3´-CTTAAG-5´

10. Write conventional nomenclature of EcoRI.

SECTION-B 5x2=10

11. Name two main steps which are collectively referred to as down
streaming process. Why is this process significant?

12. Mention two classes of restriction enzymes. Suggest their respective


roles.

13. Represent diagrammatically the E. coli. Cloning vector


βPBR 322.

14. How is gene Z used as a marker?

15. How are competent cells prepared by the action of


CaCl2?
SECTION-C 2x5=10

16. What is a polymerase chain reaction? What are the


steps involved? Mention its applications.
Or
Mention any three vector-less methods that are used to
introduce recombinant DNA into a competent host cell.

17. Describe the various steps involved in Recombinant


DNA technology with the help of a well labelled
Diagram?
Or
What are Restriction enzyme? Why do bacteria have
these restriction enzymes. Show diagrammatically a
restriction enzyme its recognition & the product it
produces?

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