Community Post c: Biotechnology & it’s application

1. 95% of the existing transgenic animals are

A. Fish

B. pigs

C. sheep

D. mice

2. Which one of the following is not used as bio-fertiliser?

A. Bacillus thuringiensis

B. Anabaena

C. Nostoc

D. Rhizobium

3. Hirudin is

A. A protein produced by Hordeum vulgare, which is rich in lysine

B. a toxic molecule isolated from Gossypium hirsutum, which

reduces human fertility

C. a protein produced from transgenic Brassica napus which

prevents blood clotting

D. an antibiotic produced by a genetically engineered bacterium

Escherichia coli.
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4. C-peptide of human insulin is:

A. Not a part of mature insulin molecule

B. Responsible for the formation of disulphide bridges

C. Removed during maturation of pro- insulin to insulin

D. Both (a) and (c)

5. Transgenic animals have been successfully used for

producing

A. Transgenic mice for testing safety of polio vaccine before use

in humans.
B. Transgenic models for studying new treatments for certain
cardiac diseases.
C. Transgenic cow rosie which produces high fat milk for making
ghee.
D. Animals like bulls for farm work as they have super power.

6. Main objective of producing herbicide resistant GM crops

is to:

A. Encourage ecofriendly herbicides.

B. Reduce herbicide accumulation in food articles for health

safety.

C. Eliminate weeds from fields without the use of manual labour.

D. Eliminate weeds from the fields without the use of herbicides.

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7. Strains of Bacillus thuringiensis are used in producing

A. Bioinsecticidal plants

B. Biominerals

C. Biometallurgical techniques

D. Biofertilisers

8. The transgenic animals are those which have

A. Foreign RNA in all its cells

B. foreign DNA in some of its cells

C. foreign DNA in all its cells

D. both (a) and (c)

9. Early detection of a disease is possible by

A. PCR

B. gene therapy

C. recombinant DNA technology and ELISA

D. both (a) and (c)

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10. For effective treatment of a disease

A. Early diagnosis is required but understanding of its

pathophysiology is not

B. early diagnosis is not required but understanding of its

pathophysiology is

C. early diagnosis and understanding of its pathophysiology

is required

D. neither early diagnosis nor understanding of its

pathophysiology is required

11. GEAC stands for

A. Genome Engineering Action Committee

B. Genetic Engineering Approval Committee

C. Ground Environment Action Committee

D. Genetic and Environment Approval committee

12. a-1 antitrypsin is

A. An antacid

B. An enzyme

C. used to treat arthritis

D. used to treat emphysema

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13. Interference RNA is derived from-

A. dsRNA

B. SSRNA

C. dsDNA

D. SSDNA

14. Maturation of proinsulin into insulin takes place after

A. Joining of C peptide

B. Removal of C peptide

C. Removal of disulphide bridge

D. Addition of disulphide bridge

15. RNA interference involves

A. Synthesis of mRNA from DNA

B. Synthesis of cDNA from RNA using reverse transcriptase

C. Silencing of specific mRNA due to complementary RNA

D. Interference of RNA in synthesis of DNA

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16. What is true about Bt toxin?

A. Bt protein exists as active toxin in the Bacillus

B. Activated toxin enters ovaries of pest to sterlise it&prevent

its multiplication

C. The concerned Bacillus has antitoxins.

D. The inactive protoxin gets converted into active form in the

insect

17. The vector DNA is used to

A. Cut the alien DNAat specific locations

B. Transfer the alien DNA into host organism

C. Join the cut DNA molecules

D. Identify DNA with desirable genes

18. Which of the following method to induce alien DNA is

found suitable for Plants:

A. Gene gun

B. Micro-injection

C. Both a and b

D. None of the above

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19. In the restriction enzyme EcoR I the letter ‘R’ refers to
the name of the

A. Species

B. Genus

C. Variety

D. Strain

20. EcoR I cut the two strands of DNA at specific

palindromic sites to form

A. Sticky end on one strand and blunt end on another

B. Blunt end on both the strands

C. Sticky end on both the strands

D. Sticky end in 5’→ 3’ strand and blunt end on 3’ 5’ strand

21. DNA fragments separated using AGE appear as

A. Orange bands after exposing to IR radiations

B. Blue bands after exposing to UV radiations

C. Orange bands after exposing to visible light

D. Orange bands after exposing to UV radiations

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22. The function of a selectable marker is

A. Eliminating transformants and permitting non transformants

B. Identify ori site

C. Elimination of non transformants and permitting

transformants

D. To destroy recognition sites

23. The introduction of T-DNAinto plants involve [Re-Test

2015]

A. Allowing the plant roots to stand in water

B. Infection of the plant by Agrobacterium tumefaciens

C. Altering the pH of the soil, then heat shocking the plants

D. Exposing the plants to cold for a brief period

24. The technique of bombarding plant cells with high

velocity microparticles of gold or tungsten, coated with
DNA, is

A. Microinjection

B. Biolistic method

C. Heat shock method

D. By disarmed pathogen vector

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25. Mark the odd one out:

A. Gene Cloning

B. Making multiple identical copies of any template DNA

C. Gene transfer

D. Molecular cloning

26. In RDT, the term vector refers to

A. Plasmids that can transfer foreign DNAinto a living cell

B. Plasmids that can cut DNA at specific bases

C. Plasmids that can join DNA at specific bases

D. Plasmids that can degrade harmful proteins

27. Who among the following was awarded Nobel Prize

for development of PCR technique?

A. Herbert Boyer

B. Kary Mullis

C. Rene Descartes

D. Andrew Fire

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28. Which of the following should be chosen for best
yield if one were to produce a recombinant protein in
large amounts?

A. Laboratory flask of largest capacity

B. A stirred tank bioreactor without inlets and outlets

C. A continuous culture system

D. Any of these

29. Which one of the following is a case of wrong

matching? [AIPMT 2012 Pre]

A. Callus – Unorganised mass of cells produced in tissue

culture
B. Somatic hybridisation – Fusion of two diverse cells
C. Vector DNA – site for t-RNA synthesis
D. Micropropagation – In vitro production of plants in large
numbers

30. For transformation, micro particles coated with DNA

to be bombarded with gene gun are made up of: [AIPMT
2012 Pre]

A. Gold or Tungsten

B. Silver or Platinum

C. Platinum or Zinc

D. Silicon or Platinum
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31. There is a restriction endonuclease called EcoRI.
What does “co” part in it stand for? [AIPMT 2011 Pre]

A. coli

B. Colon

C. Coelom

D. Coenzyme

32. The core technique(s) that enabled the birth of

modern biotechnology is/are

A. Genetic engineering

B. Maintenance of sterile ambience in chemical engineering

processes

C. Both a & b

D. None of the above

33. Stirred tank bioreactors have been designed for

[Phase II 2016]

A. Addition of preservatives to the product

B. Purification of the product

C. Ensuring anaerobic conditions in the culture vessel

D. Availability of oxygen throughout the process

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34. Isolation of DNA from a fungal cell involves the use of
enzyme

A. Lysozyme

B. Chitinase

C. Cellulase

D. Protease

35. During PCR, pairing the primers to ssDNA segment is

called

A. Denaturation

B. Extension

C. Polymerisation

D. Annealing

36. A student while extracting DNA from Aspergillus

fungus requires ____ enzyme to break open the cell wall.

A. Cellulase

B. Lysozymes

C. Pectinase

D. Chitinase

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37. Find the WRONG statements.
i. To cut DNA with restriction enzyme it needs to be pure
form, .free from RNA, protein, polysaccharides and lipids
ii. RNA can be removed by protease enzyme.
Iii. DNA separated as suspension can be removed by
spooling
iv. DNA can be precipitated by adding warm ethanol
The correct answer is

A. I, ii
B. iii, iv
C. I, iii
D. ii, iv

38. DNA precipitation out of a mixture of biomolecules

can be achieved by treatment with: [2019]

A. Methanol at room temperature

B. Chilled chloroform
C. Isopropanol
D. Chilled ethanol

39. Identify the steps involved in PCR

a. Denaturation
b. Annealing
c. Extension
d. Downstream processing

A. b,d,c
B. a,d,b
C. a,b,c
D. a,c

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40. If we have to break open the cell to release DNA
along with other macromolecules, which of the following
enzyme is used to break bacterial cell wall?

A. Cellulase

B. Lysozyme

C. Chitinase

D. Protease

41. Cry gene is obtained from

A. Bacillus thuringiensis

B. Clostridium welchi

C. Bacillus subtilis

D. E.coli

42. Bt toxin is harmful to insects like

A. Lepidoterans (tobacco budworm, armyworms)

B. Coleopterans (beetles)

C. Dipterans (flies and mosquito)

D. All of the above

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43. Statement I: biowar is the use of biological weapons
against humans and/or their crops and animals
Statement II: biopatent is the exploitation of
bioresources of other nations without proper
authorisation

A. Both statement I & II are correct

B. Both statement I & II are incorrect
C. Statement I is correct but statement II is incorrect
D. Statement II is correct but statement I is incorrect

44. Molecular diagnosis of diseases involves the use of

A. PCR

B. rDNA technology

C. ELISA

D. All of the above

45. The genetic defect, adenosine deaminase (ADA)

deficiency may be cured permanently by

A. Administering adenosine deaminase through injection

B. Bone marrow transplantation

C. Enzyme replacement therapy

D. Introducing isolated gene producing ADA into cells are

early embryonic stages

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46. Silencing of mRNA has been used in producing
transgenic plants resistant to

A. Bollworms

B. Nematodes

C. White rusts

D. Bacterial blights

47. Bt toxin kill the larvae of certain insects

A. binding toxin on mid gut epithelial cells, creating pores,

leading to swelling

B. By stopping transcription of larval cells

C. By altering central dogma taking place in the cells of gut of

larval

D. By stopping protein synthesis

48. Ass: insect resistant transgenic cotton has been

produced by inserting Bt gene
Reason: the Bt gene is derived from a bacterium

A. If both Ass & Rs are correct & the Rs is correct explanation

of ass
B. If both Ass & Rs are correct but Rs is not correct
explanation of Ass
C. If the Ass is correct but Rs is incorrect
D. If both Ass & Rs are incorrect
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49. 95% of the existing transgenic animals are

A. Fish

B. pigs

C. sheep

D. mice

50. Which one of the following is not used as

biofertiliser?

A. Bacillus thuringiensis

B. Anabaena

C. Nostoc

D. Rhizobium

51. C-peptide of human insulin is

A. A part of mature insulin molecule

B. Removed after maturation of pro insulin

C. Removed during maturation of pro insulin to insulin

D. Responsible for its biological activity

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52. In India, the organization responsible for assessing
the safety of introducing genetically modified organisms
for public use in:

A. Indian Council of Medical Research (ICMR)

B. Council for Scientific and Industrial Research (CSIR)

C. Research committee on Genetic Manipulation (RCGM)

D. Genetic Engineering Approval Committee (GEAC)

53. In 1997, which company got patent rights on Basmati

rice through the US patent and trademark office:

A. An Indian company

B. An Australian company

C. An African company

D. An American company

54. Expand GEAC:

A. Genetically Engineered Approval Community

B. Genetic Engineering Approval Community

C. Genetically Engineered Agro Community

D. Genetic Engineering Approval Committee

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55. α-1 antitrypsin is

A. An antacid

B. An enzyme

C. used to treat arthritis

D. used to treat emphysema

56. Eli Lilly was… … .company.

A. An Indian

B. An American

C. A Chinese

D. A Japanese

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 Answers

1)D 25)C 49)D

2)A 26)A 50)A
3)C 27)B 51)C
4)D 28)C 52)D
5)A 29)C 53)D
6)B 30)A 54)D
7)A 31)A 55)D
8)C 32)C 56)B
9)D 33)D
10)C 34)B
11)B 35)D
12)D 36)D
13)A 37)D
14)B 38)D
15)C 39)C
16)D 40)A
17)B 41)A
18)A 42)D
19)D 43)C
20)C 44)D
21)D 45)D
22)C 46)B
23)B 47)A
24)B 48)B
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