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ProductSheet NGS UMIAdapterSystem 31JAN22 Rev1.0

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90 views2 pages

ProductSheet NGS UMIAdapterSystem 31JAN22 Rev1.0

Uploaded by

kairaadinata30
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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PRODUCT SHEET

Twist UMI Adapter System


Enhanced sensitivity for rare variant detection

KEY BENEFITS Low-input sample sources, such as cfDNA, are increasingly common in the research and
development of potential cancer diagnostic tools. Sequencing analysis of these sample
• Enables confident detection of low
types requires robust methods to confidently detect low frequency alleles. In these cases,
frequency variants in cancer research
unique molecular identifiers (UMIs) may be added to the standard NGS workflow. UMIs
• Matched UMI adapters improve offer increased sensitivity in quantitative variant identification where the data may be
sensitivity by reducing false positives impacted by confounding PCR duplicates.
• Fully compatible with Twist’s target
The Twist UMI adapter system fits seamlessly with Twist’s Mechanical Fragmentation
enrichment solutions
Library prep kit to produce sequencing-ready libraries compatible with Illumina systems.
• Available in 16 or 96 sample The 5 basepair matched UMI adapters are first ligated to the input molecules, and
configurations subsequently PCR amplified with Twist’s Unique Dual-Indexed primers. The resulting
indexed libraries enable post-sequencing consensus building, which offers better
detection sensitivity compared to standard methods. Twist’s UMI adapters can be
easily swapped for Twist’s universal adapters, without requiring significant workflow
modifications.
Engineered for optimal performance with heterogenous sample types, the system
consists of:
• Twist Unique Molecular Identifier (UMI) Adapters: Matched adapters compatible
with all ‘T-A’ overhang workflows.

• Twist 10 bp Unique Dual-Indexed (UDI) Primers: Designed with unique dual indices
for each mixed primer pair and provided in a 96-well plate format suitable for
automation applications.

MECHANICAL
FRAGMENTATION
FRAGMENTATION

END REPAIR P
P

ATAILING P A
A P

UMI ADAPTER T P A P
LIGATION P A P T

UDI PRIMER PCR


AMPLIFICATION
LIBRARY AMPLIFICATION

FOR RESEARCH-USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.


PRODUCT SHEET | TWIST BIOSCIENCE

Greater sensitivity in variant detection


Twist’s UMI adapter system offers a research tool for consensus building that removes errors from PCR duplicates.

NO E R R O R C O R R E C T I O N
1 0 2
UM I E R R O R C O R R E C T I O N
E R R O R P ROB AB ILIT Y (FROM P HRED SC ORE)

1 0 3

1 0 4

1 0 5

1 0 6

1 0 7

1 0 8

1 0 9
0 5 ,0 0 0 1 0 ,0 0 0 15,000 20,000 25,000 30,000 35,000
P OS I T I O N A L O NG C A PT UR E S PA C E

Estimated per-base error rate for non-error-corrected reads (gray) and error-corrected reads (green) measured from target enrichment
of a synthetic cfDNA standard. After sequencing, reads were downsampled to 20,000x coverage and error correction was performed
using duplex collapse, requiring at least one read from each original strand.

Compatible with Twist NGS workflows


Twist’s comprehensive NGS toolkit enables you to take your low input sample from DNA to data. Libraries generated with Twist’s UMI
adapter system can be further prepared for targeted sequencing with Twist’s custom or fixed target enrichment panels.

S AMPL E PR E P L I BR A R Y P R E P TARG ET ENRIC HMENT SEQUENC ING A N A L Y S IS

Twist UMI Adapters are a component of the Twist ORDERING INFORMATION


portfolio of research use only (RUO) products for NGS 105040: Twist UMI Adapter System TruSeq Compatible, 16 Samples
Target Enrichment. RUO products are not intended 105041: Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate A
for use in diagnostic procedures. 105042: Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate B
105043: Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate C
LE ARN MORE
105044: Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate D
twistbioscience.com/ngs
sales@twistbioscience.com 105094: Twist UMI Adapters - TruSeq Compatible, 96 Samples

Twist Bioscience, the various Twist logos, and the Twist NGS logo used herein are trademarks of Twist Bioscience Corporation. All other trademarks are the property of their respective owners.
© 2022 Twist Bioscience Corporation. All rights reserved.

DOC-001280 RE V 1.0

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