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ExplorativeXylitolDetection 2018DecFahmi

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Annabell Francisco
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© © All Rights Reserved
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net/publication/333631558

Explorative Xylitol Detection from Lignocellulosic Biomass Fermentation of


Coconut Husk Using HPLC C18 Column

Article · December 2018

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4 authors, including:

Mariam-Aisha Fatima Nurain Nasrudin


Emma Ihsan Consultancy Management and Science
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Explorative Xylitol Detection from Lignocellulosic Biomass
Fermentation of Coconut Husk Using HPLC C18 Column

Mariam-Aisha Fatima, Muhammad Fahmi Mehdin, Nurain Nasrudin,


Faculty of Health and Life Sciences, Management & Science University,

Neelam Shahab
Industrial Biotechnology Research Centre, SIRIM Bhd.

Corresponding author: Mariam-Aisha Fatima. Email: mariam_aisha@msu.edu.my

Abstract
Lignocellulosic biomass, found in wooden plant husks is a potent renewable material source which can be
utilised to form various chemicals and biomaterials including polyols such as xylitol. Xylitol has been used
commercially as an alternative to sucrose in many products as bulk sweetener in non-cariogenic
confectionery as well as in diabetic diets and solutions for parenteral nutrition. Therefore, this study aims
to optimise separation parameters of the Shimadzu high performance liquid chromatography (HPLC)
(Model No: LC-20A) and quantify the potential of coconut husk as substrate for Candida albicans in
producing xylitol using HPLC. Pretreatment to depolymerise components of biomass i.e. cellulose,
hemicellulose and lignin were done using dilute acid hydrolysis method which yielded fermentable
reducing sugars, xylose. Xylose is a monosaccharide with an aldehyde functional group, a reducing sugar
which is then utilised and fermented by the yeast Candida albicans to form xylitol. In this study, the media
used for fermenting pretreated coconut husk and Candida albicans is a synthetic defined (SD) minimal
broth. Growth curves against concentration of reducing sugar were plotted to determine utilisation and
production trends with specific mixes of carbon sources. The presence of reducing sugars were tested using
3,5-dinitrosalicylic acid (DNS) assay in pretreated coconut husk yielded 13.22 g/L. In order to analyse the
product with HPLC, an existing protocol is modified for Hypersil GOLD™ C18 column with acetonitrile as
the mobile phase. Results obtained from HPLC analyses using developed protocol suggested the formation
of xylitol from the fermentation of pretreated husk by Candida albicans. These can be followed by
purification of fermented media in obtaining a better separation of contaminating peaks.

Keywords
Lignocellulosic biomass, Xylitol, HPLC Shimadzu, Fermentation, Reducing sugar, Candida albicans, Hypersil
GOLD™ C18 column

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