Fungi

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FUNGI

Fungi are eukaryotic, heterotrophic organisms belonging to the kingdom Fungi. They
are distinct from plants, animals, and bacteria, as they have a unique mode of nutrition,
structural characteristics, and reproduction.

Morphology and Key Characteristics

1. Cell Structure:
o Fungi are eukaryotic with a well-defined nucleus and membrane-bound
organelles.
o Their cell walls are composed of chitin, unlike plants which have cellulose.
2. Body Organization:
o Unicellular (e.g., Saccharomyces cerevisiae) or multicellular with a
filamentous structure (e.g., molds).
o The multicellular forms have a network of filaments called hyphae,
collectively forming a mycelium.
3. Nutrition:
o Fungi are heterotrophic, obtaining nutrients by absorption.
o They can be saprophytic (feeding on dead organic matter), parasitic, or
mutualistic (e.g., mycorrhizae).
4. Reproductive Structures:
o Specialized structures are formed during sexual and asexual reproduction,
such as spores, sporangia, and fruiting bodies.
5. Growth Environment:
o Thrive in a variety of environments, often in moist, dark places.
o Can grow on a wide range of substrates, including organic matter, soil, and
decaying plants/animals.
6. Metabolic Versatility:
o Capable of breaking down complex organic molecules, playing a crucial role
in nutrient cycling.
7. Symbiosis and Pathogenicity:

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o Some fungi form mutualistic relationships (e.g., lichens, mycorrhizae), while
others can be pathogenic to plants, animals, or humans.

Classification of Fungi

1. Phycomycetes (Zygomycota):
o Non-septate hyphae.
o Asexual spores: Sporangiospores.
o Sexual reproduction: Zygospores.
o Example: Rhizopus, Mucor.
2. Ascomycetes (Sac Fungi):
o Septate hyphae.
o Asexual spores: Conidia.
o Sexual reproduction: Ascospores in asci.
o Example: Saccharomyces, Penicillium, Aspergillus.
3. Basidiomycetes (Club Fungi):
o Septate hyphae.
o Sexual spores: Basidiospores produced on basidia.
o Example: Mushrooms, Puccinia.
4. Deuteromycetes (Fungi Imperfecti):
o No known sexual stage.
o Asexual spores: Conidia.
o Example: Candida, Alternaria.

Reproduction in Fungi

Fungi reproduce through asexual and sexual methods:

Asexual Reproduction:

1. Sporulation:
o Formation of asexual spores like sporangiospores, conidia, or chlamydospores.
2. Fragmentation:
o Hyphae break into fragments, each capable of growing into a new mycelium.
3. Budding:
o Common in unicellular fungi like Saccharomyces cerevisiae.

Sexual Reproduction:

1. Plasmogamy:
o Fusion of the cytoplasm of two compatible mating types.
2. Karyogamy:
o Fusion of nuclei to form a diploid zygote.
3. Meiosis:
o Reduction division to form haploid sexual spores (e.g., ascospores,
basidiospores).

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4. Spore Dispersal:
o Spores are released into the environment for germination.

Cultivation of Fungi

Fungi can be cultivated for various applications such as food production, enzyme production,
and research.

1. Substrates:
o Fungi grow well on nutrient-rich substrates like PDA (Potato Dextrose Agar),
SDA (Sabouraud Dextrose Agar), or natural materials like wheat bran,
cornmeal, and molasses.
2. Growth Conditions:
o Temperature: Optimal growth at 20–30°C for most fungi.
o pH: Slightly acidic (pH 4–6) is preferred.
o Moisture: Essential for fungal growth.
3. Sterilization:
o Media and equipment should be sterilized to prevent contamination by other
microbes.
4. Inoculation:
o Fungal spores or hyphae are transferred onto the growth medium under sterile
conditions.
5. Incubation:
o Plates or flasks are incubated under controlled temperature and humidity to
allow fungal growth.
6. Harvesting:
o Biomass or secondary metabolites are collected based on the purpose (e.g.,
antibiotics, enzymes).
7. Industrial Scale:
o Fungi like Aspergillus niger and Penicillium chrysogenum are cultivated in
fermenters for large-scale production.

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