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Enzymes

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6 views5 pages

Enzymes

Uploaded by

ashra.aneespedia
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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ANEESPEDIA EDUCATION SUPPORT SERVICES

TEST

SUBJECT: Biology TOPIC: Enzymes


CLASS: 10 TOTAL TIME: 1 hour
BOARD: IGCSE TOTAL MARKS: 30

SECTION – A [5 X 1 = 5 Marks]

1. The pH of an enzyme-controlled reaction is decreased by a pH of 2. How does this affect the


rate of the reaction?
A. It has no effect on the rate.
B. It always decreases the rate.
C. It always increases the rate.
D. It may increase or decrease the rate.

2. Temperature affects the rate of all enzyme-controlled reactions, as shown in the graph
below.

Which row correctly describes what is happening at the points on the graph?

3. A student decided to investigate the effect of pH on the activity of the enzyme lipase. Five
different test tubes are set up. Each test tube contains a mixture of the enzyme lipase and its
substrate, a lipid. Which condition should not be kept the same in each of the five test tubes?
A. Temperature
B. Concentration of lipase

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TEST

C. Volume of lipid solution


D. pH

4. The action of an enzyme in a chemical reaction is outlined in the diagram below.

Which row of the table below is correct?

5. Starch is digested by the enzyme amylase. Four identical mixtures of starch and amylase
were kept at different temperatures. The graph below shows the time taken for the starch to
be digested at each temperature. At which temperature is the rate of reaction highest?

(a) A
(b) B
(c) C

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TEST

(d) D
SECTION – B

1. (a) Sketch the enzyme substrate reaction using the following images. [3]

(b) Enzymes are biological catalysts. Explain what is meant by the term catalyst. [1]
(c) Describe the action of enzymes. Use the following words as part of your answer: [1]

Collide, Complementary, Randomly, Recycled


2. A student investigated the effect of pH on the activity amylase on starch solution. The
student used iodine solution as an indicator to see when the amylase was active. Their results
are shown in the table below.

(a) Describe why the student used iodine solution. [1]


(b) State which of the pH values tested is the optimum for amylase. Justify your answer. [2]
(c) Another student concluded that the optimum pH was not shown in the results in Table 1.
Explain why the student concluded this. [2]

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TEST

3. The below figure represents the reactants and products in a chemical reaction and four
different enzymes (A, B, C or D).

(a) State the letter of the enzyme responsible for this reaction. [1]
(b) State what happens when a substrate with a shape that is not complementary to the
enzyme tries to enter the active site. [2]

4. (a) State one factor other than temperature that affects enzyme activity. [1]
(b) Digestive enzymes are used in chemical digestion to break down large insoluble molecules
to smaller soluble molecules. The boxes show some large insoluble molecules, some digestive
enzymes and some smaller soluble molecules that are produced during digestion. Draw one
straight line from each enzyme to the insoluble molecule it acts on. Draw another line from
each enzyme to the smaller soluble molecule that is produced. [3]

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(c) The below figure shows an enzyme in its optimum pH and in a pH that is much lower.

Describe what has happened to the enzyme. [1]


(d) Sketch an appropriate substrate for the enzyme shown in the above figure. [2]

5. During the fermentation of yeast, enzymes that break down sugar release carbon dioxide
gas. The volume of carbon dioxide released can be used to measure how fast these enzymes
are working. A student investigates if fermentation works faster at two different temperatures:
25°C or 30°C. They measured the volume of carbon dioxide released in 15 minutes. The below
figure shows the apparatus she uses.

(a) The student used a gas syringe to collect and measure the volume of carbon dioxide
released. They could have measured the volume of carbon dioxide by collecting the gas over
water in a measuring cylinder. Use the information provided in the above figure to suggest why
itis better to use a gas syringe. [2]
(b) The student’s results are shown in the below table.

(i) Calculate the mean rate of gas produced at 25°C in cm /minute. Give your answer to 1
decimal place. [2]
(ii) The teacher tells the student to repeat the results for 30°C. Explain why the teacher advised
this. [1]

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