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BIOLOGY 9700/34
Paper 3 Advanced Practical Skills 2 October/November 2024
2 hours
You must answer on the question paper.
You will need: The materials and apparatus listed in the confidential instructions
INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.
INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].
For Examiner’s Use
Total
This document has 12 pages. Any blank pages are indicated.
DC (DE/CGW) 336167/3
© UCLES 2024 [Turn over
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1 Vegetables, such as carrots, contain sugars.
Potassium manganate(VII) solution can be used to identify the presence of sugars.
The sugars change the colour of the potassium manganate(VII) solution from purple to colourless.
You will measure the time taken for potassium manganate(VII) solution to turn colourless with
sugar solutions of known concentration. You will use the results to estimate the concentration of
sugars in a carrot extract.
You are provided with the materials shown in Table 1.1.

Table 1.1
labelled contents hazard volume / cm3

S 1.0 mol dm–3 sugar solution none 40
W distilled water none 40
harmful
A sulfuric acid 20
irritant
K potassium manganate(VII) solution irritant 20

C carrot extract none 10
If any solution comes into contact with your skin, wash off immediately with cold water.
It is recommended that you wear suitable eye protection and wear gloves to protect your hands
when using A and K.
You will need to:
• prepare different concentrations of sugar solution
• record the time taken for K to become colourless (end-point) for each of the different

concentrations of sugar solution and for the carrot extract, C
• use your results to estimate the concentration of sugars in the carrot extract, C.
You will need to use proportional dilution to make five different concentrations of sugar solution.
You will need to prepare 10 cm3 of each concentration, using S and W.
Table 1.2 shows two of the concentrations of sugar solution you will use and how to prepare them.
Decide which three other concentrations of sugar solution you will use.
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(a) (i) Complete Table 1.2 to show how you will prepare the other concentrations of sugar
solution you will use.
Table 1.2
concentration of sugar
volume of S / cm3 volume of W / cm3
solution / mol dm–3
1.0 10.0 0.0
0.0 0.0 10.0

[2]
Carry out step 1 to step 11.
step 1 In the beakers provided, prepare the concentrations of sugar solution, as shown in
Table 1.2.
step 2 Label the test-tubes with the concentrations of sugar solution prepared in step 1.
step 3 Put 1 cm3 of 1.0 mol dm–3 sugar solution into the appropriately labelled test-tube.
step 4 Repeat step 3 with each of the other concentrations of sugar solution.
step 5 Put 1 cm3 of A into each of the test-tubes. Shake gently to mix.
The reaction will start as soon as you put K into the test-tubes (step 6). Keep the timer running
continuously until the end of step 7.
step 6 Put 1 cm3 of K into each of the test-tubes and start timing. Shake gently to mix.
step 7 Measure the time taken for each concentration to reach the end-point. As each end-
point is reached record the time taken in (a)(ii).
If an end-point has not been reached after 600 seconds, record the time as ‘more
than 600’.
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(ii) Record your results in an appropriate table.

[5]

step 8 Label a test-tube C and put 1 cm3 of C into this test-tube.
step 9 Put 1 cm3 of A into the test-tube. Shake gently to mix.
step 10 Put 1 cm3 of K into the test-tube and start timing. Shake gently to mix.
step 11 Measure the time taken to reach the end-point. Record the time taken in (a)(iii).
(iii) State the time taken to reach the end-point for C.
time taken ......................................................... [1]

(iv) Estimate the concentration of sugars in C.
concentration of sugars in C ........................................... mol dm–3 [1]
(v) Suggest how the procedure could be modified to improve the accuracy of your estimate
in (a)(iv).
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [3]
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(vi) A student used the same procedure to compare the concentration of sugars in three
vegetables: carrot, potato and onion.
State one variable that needs to be standardised in the procedure.
...........................................................................................................................................
..................................................................................................................................... [1]
(vii) Suggest how the student could extend this investigation to estimate the concentration of
starch in a vegetable extract.
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [2]
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(b) Bananas produce ethylene gas which causes them to ripen. The production of ethylene gas
continues after the bananas are removed from the plant (harvested).
The ethylene gas decreases the post-harvest life of the bananas. The post-harvest life is the
time after harvesting when the bananas are suitable to eat.
Potassium manganate(VII) can be used to increase the post-harvest life of the bananas by
oxidising the ethylene gas to form water and carbon dioxide.
An experiment was carried out to determine the effect of different quantities of potassium
manganate(VII) on the post-harvest life of bananas.

The results are shown in Table 1.3.
Table 1.3
mass of potassium mean post-harvest life

manganate(VII) / g / days
0 9.30
2 11.25
4 12.60

6 13.65
8 11.45

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(i) Plot a graph of the data in Table 1.3 on the grid in Fig. 1.1.
Use a sharp pencil.

Fig. 1.1
[4]
(ii) Use your graph in Fig. 1.1 to predict the post-harvest life of bananas if the mass of
potassium manganate(VII) is 6.8 g.
Show on your graph how you obtained your answer.
post-harvest life of bananas = ................................................. days [2]
(iii) Suggest why the post-harvest life of bananas decreases when more than 6 g of potassium
manganate(VII) is used.
...........................................................................................................................................
..................................................................................................................................... [1]
[Total: 22]
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2 L1 is a slide of a stained transverse section through a plant root.
(a) (i) Draw a large plan diagram of the whole section on L1.
Use a sharp pencil.
Use one ruled label line and label to identify the endodermis.

[5]
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(ii) Observe the epidermis of the root on L1 and the layer of cells beneath.
Select a group of four adjacent cells. This group must include two cells from the
epidermis and two cells from below the epidermis.
Each cell must touch at least two of the other cells.
• Make a large drawing of this group of four cells.
• Use one ruled label line and label to identify the cell wall of one epidermis cell.
[5]
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(b) Fig. 2.1 is a photomicrograph of a stained transverse section through a different plant.

Fig. 2.1
Identify three observable differences, other than colour, between the section on L1 and the
section in Fig. 2.1.
Record these three observable differences in Table 2.1.
Table 2.1
feature L1 Fig. 2.1

[4]
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(c) Fig. 2.2 is the same photomicrograph as that shown in Fig. 2.1.
B
525 μm
Fig. 2.2
Use the scale bar on Fig. 2.2 and the line A–B to calculate the actual diameter of the section
in Fig. 2.2.
Show your working, including units.

actual diameter = ......................................................... [4]
[Total: 18]
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reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.
To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge
Assessment International Education Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download
at www.cambridgeinternational.org after the live examination series.
Cambridge Assessment International Education is part of Cambridge Assessment. Cambridge Assessment is the brand name of the University of Cambridge
Local Examinations Syndicate (UCLES), which is a department of the University of Cambridge.
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* 0000800000001 *

Cambridge International AS & A Level

You must answer on the question paper.

For Examiner’s Use

This document has 12 pages. Any blank pages are indicated.

DO NOT WRITE IN THIS MARGIN

1 Vegetables, such as carrots, contain sugars.

Potassium manganate(VII) solution can be used to identify the presence of sugars.

You are provided with the materials shown in Table 1.1.

DO NOT WRITE IN THIS MARGIN

labelled contents hazard volume / cm3

DO NOT WRITE IN THIS MARGIN

You will need to:

• prepare different concentrations of sugar solution

DO NOT WRITE IN THIS MARGIN

You will need to prepare 10 cm3 of each concentration, using S and W.

0.0 0.0 10.0

Carry out step 1 to step 11.

DO NOT WRITE IN THIS MARGIN

(ii) Record your results in an appropriate table.

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN

step 9 Put 1 cm3 of A into the test-tube. Shake gently to mix.

(iii) State the time taken to reach the end-point for C.

time taken ......................................................... [1]

DO NOT WRITE IN THIS MARGIN

concentration of sugars in C ........................................... mol dm–3 [1]

State one variable that needs to be standardised in the procedure.

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN

mass of potassium mean post-harvest life

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN

Use a sharp pencil.

Show on your graph how you obtained your answer.

post-harvest life of bananas = ................................................. days [2]

DO NOT WRITE IN THIS MARGIN

2 L1 is a slide of a stained transverse section through a plant root.

Use a sharp pencil.

DO NOT WRITE IN THIS MARGIN

Each cell must touch at least two of the other cells.

• Make a large drawing of this group of four cells.

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN

Record these three observable differences in Table 2.1.

feature L1 Fig. 2.1

DO NOT WRITE IN THIS MARGIN

Show your working, including units.

actual diameter = ......................................................... [4]

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN

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