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School Project: Casein Content in Milk

INDEX

> INTRODUCTION

> AIM

> REQUIREMENTS

> THEORY

> PROCEDURE

> OBSERVATION

> CONCLUSION

> BIBLIOGRAPHY

INTRODUCTION

Natural milk is an opaque white fluid secreted by the mammary glands of female mammals. The

main constituents of natural milk are protein, carbohydrates, minerals, vitamins, fats, and water,

making it a complete balanced diet. Fresh milk is sweetish in taste. However, when kept for a long

time at a temperature of 5°C, it becomes sour due to bacteria present in the air. These bacteria

convert lactose of milk into lactic acid. In acidic conditions, casein starts separating out as a

precipitate.

AIM

Aim is to determine the quality of casein present in different samples of milk.

REQUIREMENTS

Beakers (250 ml), filter paper, glass rod, weigh box, filtration flask, Buchner funnel, water pump, test

tube, porcelain dish, burner, different samples of milk, 1% acetic acid solution, saturated Ammonium

Sulphate solution.

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School Project: Casein Content in Milk
THEORY

Natural milk is an opaque fluid secreted by the mammary glands of female mammals. Its main

constituents are protein, carbohydrates, minerals, vitamins, fats, and water, making it a complete

balanced diet. Fresh milk is sweetish in taste but turns sour over time due to bacterial action.

PROCEDURE

1. Take a clean dry beaker, put 20 cc of goat's milk, and add 20 ml of saturated Ammonium

Sulphate solution.

2. Stir the solution slowly; fat along with casein will precipitate out.

3. Filter the solution and transfer the precipitate to another beaker.

4. Add about 30 ml of water to the precipitate; casein dissolves in water forming a milky solution.

5. Heat the milky solution to about 40°C and add 1% acetic acid dropwise.

6. Filter the precipitate, wash with water, dry it, and weigh it on a pre-weighed watch glass.

7. Repeat the experiment with other milk samples.

OBSERVATION

Sample No | Source | Content of Protein | % of Casein

1 | Cow Milk | 0.60 | 3.00

2 | Goat Milk | 0.65 | 3.25

3 | Buffalo Milk | 0.85 | 4.20

4 | Amul Milk | 0.75 | 3.88

CONCLUSION

Different samples of milk contain different percentages of casein.

BIBLIOGRAPHY

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School Project: Casein Content in Milk
Internet Sources:

1. www.google.com

2. www.yahoo.com

3. www.wiki.com

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