Clinical pathology
Clinical pathology
Clinical pathology
OWERRI
P.M.B 1526 OWERRI
IMO STATE
A REPORT ON
SCHEME (SIWES)
DONE AT
FLEX ENTREPRISE NIGERIA
NO 110 ADEKUNLE STREET, IKOYI LAGOS STATE
WRITTEN BY
SUBMITTED TO
SEPTEMBER, 2023
DEDICATION
To the Almighty God, I dedicate this report for being my spiritual supervisor, for
his grace to complete the programmed and his faithfulness all through the period.
ACKNOWLEDGEMENT
I thank the management of Flex Enterprise Nigeria for the opportunity and
privilege granted to me to carry out my industrial training, for the experience was
allowed to acquire and making my training worthwhile.
Also, I dedicate this work to my wonderful family for their countless support
morally and financially.
This report covers the entire test that has being carried out in the Clinic during my
industrial training. It is a comprehensive description of the hospital, the various
departments and functions. This report work also talks on SIWES and its aim and
objectives. It also talks on the laboratory safety precautions to avoid accident in the
clinic.
Table of Contents
CHAPTER ONE.............................................................................................................................................1
STUDENT INDUSTRIAL WORK EXPERIENCE SCHEME (SIWES)..................................................................1
1.1 BACKGROUND STUDY.......................................................................................................1
1.2 OBJECTIVES OF SIWES.......................................................................................................2
1.3 THE NEED FOR INDUSTRIAL TRIANING.............................................................................3
1.4 BENEFITS OF INDUSTRIAL TRAINING................................................................................3
1.5 BODIES INVOLVED IN THE MANAGEMENT OF SIWES.....................................................4
CHAPTER TWO.............................................................................................................................................9
2.0. LABORATORY:.........................................................................................................................9
2.0.1 LABORATORY EQUIPMENT AND THEIR USES:.........................................................9
LABORATORY EQUIPMENTS......................................................................................................10
2.0.2 LABORATORY PRECAUTIONS:...................................................................................11
2.0.3: LABORATORY SAFETY SIGNS..........................................................................................12
2.0.4 DIFFERENT DEPARTMENT IN THE LABORATORY:................................................13
3.1 PHLEBOTOMY LABORATORY:.......................................................................................13
3.1.2 COLLECTION OF SPECIMEN:.......................................................................................13
3.2 HEMATOLOGY/SEROLOGY LABORATORY.................................................................16
3.2.1 BLOOD GROUPING........................................................................................................17
3.2.2 PREGNANCY TEST.........................................................................................................18
CHAPTER FOUR..........................................................................................................................................20
4.0 MICROBIOLOGY:...............................................................................................................20
4.0.1 WIDAL TEST:..................................................................................................................20
4.0.2 URINALYSIS....................................................................................................................21
4.0.3 HIGH VIGINAL SWAB (HVS) TEST..............................................................................23
CHAPTER 5.................................................................................................................................................24
5.1 PROBLEMS ENCOUNTERD DURING MY SIWES PROGRAM.............................................................24
5.2 CONCLUSION...................................................................................................................................24
CHAPTER ONE
1.0 INTRODUCTION
The scheme also affords students the opportunity of familiarizing and exposing
themselves to the needed experience in handling equipment and machinery that are
not usually available in their institutions.
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1.1 BACKGROUND STUDY
The students Industrial Work Experience Scheme (SIWES) was established in
1973/1974 session. Prior to the establishment of the scheme, there was a growing
concern among our industrialist that graduates of our institutions of higher learning
lacked adequate background studies preparatory for employment in the industries.
It is against this background that the rationale for initiating and designing the
scheme was hinged. Consequently, the scheme affords students the opportunity of
familiarizing and exposing themselves to the needed experience in handling
equipment and machinery that are usually not available in the institutions.
The growing concern among our industrialist that graduates of our institutions of
higher learning lack adequate practical background studies preparatory for
employment in industries, led to the formation of Students Industrial Work
Experience Scheme (SIWES) by ITF in 1993/1994. (Information and Guideline for
SIWES 2002) ITF has as one of its key functions; (1) to work as cooperative entity
with industry and commerce where students in institutions of higher learning can
undertake mid-career work experience attachment in industries which are
compatible with students’ area of study (Okorie 2002, in Asikadi 2003).
The scheme was designed to expose students to industrial environment and enable
them to develop occupational competencies so that they can readily contribute their
quota to national economic and technological development after graduation. The
scheme also enables students to acquire knowledge, skill and experience to
perform jobs in their respected fields.
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a. Provide an avenue for students in institution of higher learning to acquire
industrial skills and experience in their approved course of study.
b. Prepare students for the industrial works situation which they are likely to
meet after graduation.
Both education and training are important, there cannot be effective education
without dome training input and there cannot be effective training without some
educational input. The productive individual, particularly in this millennium, must
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be able to combine the outcomes from the two forms of learning (Know-How
ability and Do-How capability) for the production of goods and services. This
requirement is particularly crucial for individuals pursuing careers in science,
engineering and technology.
Several other benefits can accrue to students who participate in industrial training.
These include the following:
Enabling SET students appreciate work methods and gain experience in handling
equipment and machinery which may not be available in their institution.
Preparing students for employment and making the transition from school to the
world of work easier after graduation.
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Enhancing student’s contacts with potential employers while on training.
Enabling students bridge the gap between the knowledge acquired in institutions
and the relevant production skills (RPSs) required in work organizations.
Making SET students appreciate the role of their professions as the creator of
change and wealth and indispensable contributors to growing the economy and
national development.
LAB
LAB
CHEMICAL MICROBIOLO
HEAMATOLO PATHOLOGY GY SEROLOGY
GY LAB 5 LAB LAB
TECHNICIAN TECHNICIAN
DEPARTMENTS IN THE MEDICAL LABORATORY
CENTRIFUGE
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Fig.2.1 Centrifuge
SYRINGE DESTROYER
WATERBATH
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It is use for sterilization and make liquid be at a stable temperature.
MICROSCOPE
It is use for viewing and magnification of tiny object that cannot be seen with the
naked eyes
TEST TUBE
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It is use to hold sample and reagent during test
CHAPTER TWO
2.0. LABORATORY:
A laboratory is a facility that provides controlled conditions in which
scientific or technological research, experiment and diagnosis are performed
such as medical laboratory which is a laboratory which is a laboratory where
test are carried on specimens and patient to obtain information about the
health of the patient.
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INCUBATOR: it is used to culture micro-organism at a certain
steady temperature.
CENTRIFUGE: it is used for the separation of fluids by spinning a
vessel containing materials at high speed.
BEAKERS: it is used for mixing and heating chemicals.
CONICAL FLASK: it is also used for misking and swirling of the
flask without much risk of spilling.
BOILING FLASK: it is used to hold liquids and can easily be heat.
TEST TUBES: it is used to hold small amount of samples.
PIPETTES: it is used for extremely accurate addition of liquid
LABORATORY EQUIPMENTS
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RING STAND & CLAMPS: it is used to suspend burettes, beakers,
flasks etc above the containers.
SPATULAS & SCOOPULAS: it is used for scooping solid
chemicals.
BUNSEN BURNERS: it is used for heating chemicals
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2.0.4 DIFFERENT DEPARTMENT IN THE LABORATORY:
Medical laboratory is generally divided into two sections. Each has being
subdivided into multiple units. Here are the two sections.
ANATOMIC PATHOLOGY
CLINICAL PATHOLOGY
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3.1.2 COLLECTION OF SPECIMEN:
This is also a unit where specimens are collected e.g. Of specimen collected
here are urine sample, high vagina swab, blood sample etc.
BLOOD SAMPLE: they are collected using two methods depending on the
test required.
They are:
Is a blood sample collected by pricking the skin capillaries are tiny blood vessels
near the surface of the skin that require small amount of blood from patient
materials
Methylated spirit
Wet swab
Lancets
Capillary tube
PROCEDURES:
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VENOUS BLOOD COLLECTION
Is a blood sample collected through the vein and require large amount of blood
MATERIALS USED:
Tourniquet
Syringe
Wet swab
Methylated spirit
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PROCEDURE:
The material needed for the collection of blood sample be made available
If there is any trace of vein, you tie the tourniquet above the area of
collection.
Then the patient is asked to make a fist to make the vein more prominent
Then use your wet swab to disinfect the surface area
Then use your syringe to collect the blood
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3.2.1 BLOOD GROUPING
AIM: To determine the blood group of a patient.
MATERIALS USED:
Plastic tile
Tweezer
Blood sample
Capillary tube
Reagents (anti ceral – A, B, D-)
PROCEDURES:
Collect the patient’s blood sample using a capillary tube
Drop 3 drops spaced at different intervals (blood sample)
Then drop 3 drop of anti ceral (A, B and D) respectively on each drop of
blood.
Using your tweezer, both blood sample and anti ceral then you rock for
2minutes.
Read your result
RESULT
REAGENT REAGENT REAGENT RESULT
A B D
+ + + =AB+
_ + + =B+
+ _ + =A+
_ _ _ =O-
_ _ + =O+
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3.2.2 PREGNANCY TEST
AIM: To determine or detect of a woman is pregnant or not. It can be carried out
in two different ways.
MATERIALS USED:
Test tube
Patients blood/serum
Centrifuge machine
Pregnancy test strip
PROCEDURES:
USING BLOOD SERUM
The blood sample is centrifuged to get the serum
The serum is made to migrate through the pregnancy test strip, then left
for about 20munites for results to appear
To begin the test, remove the strip from the sealed pouch.
The test strip is placed vertically (straight) into the urine sample for at least
10seconds, making sure the arrows are pointing downwards
The urine is not allowed to go above the maximum level line on the test strip
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Remove the strip from the urine and placed the strip on a clean, dry surface
Wait for colour bands to appear. If it is a positive result it will show a double
line within forty seconds but if it is a negative result it will show a single
line in five minutes
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CHAPTER FOUR
4.0 MICROBIOLOGY:
Microbiology works with mainly cultures, specimen, urine, blood and
sputum etc. also sensitivity testing is also carried out to determine whether
the pathogen is sensitive or resistant to a suggested medicine (anti-biotic)
MATERIALS:
Test tiles
Centrifuge
Widal kits
Pasteur pipette
Serum gotten from blood samples
Tweezer
Sample bottles
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PROCEDURES:
The patient’s blood samples are collected using a syringe and sterile needle
The blood sample is been put into a sample bottle containing EDTA as its
anti coagulant.
The sample bottle is kept inside the centrifuge to be spinned in other to
separate the serum from the red blood cells
Using a widal kit that contains the reagent for the test (four blue reagent for
antigen O, A-C and four pink reagent for antigen H,A-C) drops of the
respective reagent were placed on the clean white test tile on four places
each.
Using a Pasteur pipette, the plasma is placed side by side on all reagent
(antigens)
Using a tweezer or the bottom part of the pipette to properly mix the plasma
and the antigen and further rocked for five minutes.
In the process of rocking, an antigen complex is formed
Results are read or checked by the agglutination.
4.0.2 URINALYSIS
This is used to know if a patient is infected with urinary tract infection (UTI)
or not.
Sterile container
Combi 9 strip
Urine sample
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PROCEDURES:
The urine sample is been collected in a sterile container
Then you take the sample to the laboratory and mix gently
Open carefully, then take a strip of combi 9 and deep inside, leave for
30 seconds
Then you gently bring out the combi 9 strip dragging the strip on the
body of the container in order so that urine do not drop on the floor
Then read your result using the combi 9 container to compare with a
standard
OBSERVATION:
When the strip has been dipped into the urine after 30secounds and brought
out there will be a colour change in each of the nine parameters.
PARAMETERS:
Blood
Ketone
Nutrite
Ascorbic acid
Bilirubin
Urobilinogen
pH
protein
PRECAUTION
Ensure that the urine sample to be used is mid stream
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CONCLUSION:
If the patient have two plus (++) and above, of glucose in their urine are
advice to stop the intake of sugar, etc
MATERIALS USED:
Vaginal fluid
Incubator
Normal saline
Chocolate agar
Sterile swap stick
PROCEDURE:
Insert the sterile swab stick into the vagina to collect vagina fluid
Make a stick on the petri-dish containing chocolate agar and incubate
for 24hours at 370degree centigrade
OBSERVATION:
PRECAUTIONS:
Ensure that the sterile swab stick is well dipped into the vagina during
sample collection
Ensure appropriate incubation, time and temperature
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CHAPTER 5
5.2 CONCLUSION
In conclusion, as a student, I have been able to obtain the most relevant and
effective practical industrial training and experience for duration of three months
(6 months). I have been exposed to practical and activities in the laboratory.
Furthermore, an awareness of the general work place has been developed in me
and I have acquired important behavior and interpersonal skills with the
opportunity given to me to get a feel of the work environment and exposure as a
students.
Finally, I would like to say that the SIWES program is a very relevant and
necessary program for all students.
5.3 RECOMMENDATIONS
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Universities should strine to give their students industrial training forms on
time to enable them start early to search for a place of attachment
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