(For Research Use Only. Not For Use In Diagnostic Procedures!

FineTest®
Human SA (Sialic Acid) ELISA Kit

Catalogue No.: EH3736

Revision: V4.0
Size: 48T/96T

Please do not mix and use reagents from different kits or different batches. Otherwise, it might not work
properly.
Please read the manual carefully before use. Feel free to contact us if you have any questions.

Email fine@fn-test.com

Website https://www.fn-test.com/

Please provide the batch number (see kit label) for more rapid response and services.
It's strongly recommended to use this kit within the expiry date printed on the kit label.

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Technical support related documents

Standard curve and

concentration calculation
Title of Sample preparation Experimental operation TMB color rendering
software
Document guide procedure control
CurveExpert1.4(Including
tutorial)

https://www.fn-
https://www.fn- https://www.fn- https://www.fn-
test.com/content/uplo
test.com/videos/finetest test.com/videos/targete test.com/content/uploads/
Website ads/2022/06/ELISA-
-elisa-kits-operation- d-control-of-tmb- 2019/08/CurveExpert-
Sample-Preparation-
guide-competitive/ coloring/ 1.4.zip
Protocol-2022.6.6.pdf

Quick Mark

Product Features

In vitro quantitative determination of SA concentrations in serum, plasma, cell

Application
culture supernatant and other biological samples.

Reactivity Human Detection Method Competitive

Range 62.5-4000ng/ml Sensitivity 37.5ng/ml

Detection Duration 2 hours(excluding balancing and sample preparation)

Samples needed for Serum: 3 ul, Plasma: 3 ul, Cell Culture Supernatant: 50ul, cell or tissue lysate: 50ul,
single well(Max) Other liquid samples: 50ul

Specificity Specifically recognize SA, no obvious cross reaction with other analogues

Storage 2-8°C (for sealed box), please do not freeze! See kit label for expiry date

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Principle of the Assay
This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been
pre-coated with SA. During the reaction, SA in the sample or standard competes with a fixed amount of SA
on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to SA. Excess conjugate
and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each
microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate
reaction is terminated by the addition of a acid solution and the color change is measured
spectrophotometrically at a wavelength of 450nm. The concentration of SA in the samples is then
determined by comparing the OD of the samples to the standard curve. The concentration of the target
substance was inversely proportional to the OD450 value.

Kit Components and Storage

The sealed kit can be stored at 2-8 ℃. The storage condition for opened kit is specified in the table below:

No. Item Size(48T) Size(96T) Storage Condition for Opened Kit

Put the rest strips into a sealed foil

bag with the desiccant. Stored for 1
E001 ELISA Microplate(Dismountable) 8×6 8×12
month at 2-8°C; Stored for 6 month at -
20°C

Put the rest standards into a desiccant

E002 Lyophilized Standard 1vial 2vial bag. Stored for 1 month at 2-8°C; Stored
for 6 month at -20°C

Biotin-labeled
E003 30ul 60ul
Antibody(Concentrated, 100X)

HRP-Streptavidin 2-8°C (Avoid Direct Light)

E034 60ul 120ul
Conjugate(SABC, 100X)

E024 TMB Substrate 5ml 10ml

E039 Sample Dilution Buffer 10ml 20ml

E040 Antibody Dilution Buffer 5ml 10ml

E049 SABC Dilution Buffer 5ml 10ml 2-8°C

E026 Stop Solution 5ml 10ml

E038 Wash Buffer(25X) 15ml 30ml

E006 Plate Sealer 3 pieces 5 pieces

E007 Product Description 1 copy 1 copy

Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette
accurately measure and do proportional dilution.

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Required Instruments and Reagents
1. Microplate reader (wavelength: 450nm)
2. 37°C incubator (CO 2 incubator for cell culture is not recommended.)
3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable
tips(calibration is required before use.)
5. Sterile tubes and Eppendorf tubes with disposable tips
6. Absorbent paper and loading slot
7. Deionized or distilled water

Sample Collection and Storage

The following sample processing steps are concise operations. For detailed sample preparation guideline,
please refer to the Quick Mark or the link (https://www.fn-test.com/content/uploads/2022/06/ELISA-
Sample-Preparation-Protocol-2022.6.6.pdf).
1. Serum
Place whole blood sample at room temperature for 2 hours or at 2-8°C overnight. Centrifuge for 20min at
1000xg and collect the supernatant to detect immediately. Or you can aliquot the supernatant and store it at
-20°C or -80°C for future’s assay.
2. Plasma
EDTA-Na 2/K 2 is recommended as the anticoagulant. Centrifuge samples for 15 minutes at 1000×g 2-8°C
within 30 minutes after collection. Collect the supernatant to detect immediately. Or you can aliquot the
supernatant and store it at -20°C or -80°C for future’s assay. For other anticoagulant types and uses, please
refer to the sample preparation guideline.
3. Tissue Sample
Generally tissue samples are required to be made into homogenization. Protocol is as below:
3.1. Place the target tissue on the ice. Remove residual blood by washing tissue with pre-cooling PBS buffer
(0.01M, pH=7.4). Then weigh for usage.
3.2. Use lysate to grind tissue homogenates on the ice. The adding volume of lysate depends on the weight
of the tissue. Usually, 9mL PBS would be appropriate to 1 gram tissue pieces. Some protease inhibitors are
recommended to add into the PBS (e.g. 1mM PMSF ).
3.3. Do further process using ultrasonic disruption or freeze-thaw cycles (Ice bath for cooling is required
during ultrasonic disruption; Freeze-thaw cycles can be repeated twice.) to get the homogenates.
3.4. Homogenates are then centrifuged for 5 minutes at 5000×g. Collect the supernatant to detect
immediately. Or you can aliquot the supernatant and store it at -20°C or -80°C for future’s assay.
3.5. Determine total protein concentration by BCA kit for further data analysis. Usually, total protein
concentration for Elisa assay should be within 1-3mg/ml. Some tissue samples such as liver, kidney, pancreas
which containing a higher endogenous peroxidase concentration may react with TMB substrate causing false
positivity. In that case, try to use 1% H 2O2 for 15min inactivation and perform the assay again.
Notes: PBS buffer or the mild RIPA lysis can be used as lysates. While using RIPA lysis, make the PH=7.3.
Avoid using any reagents containing NP-40 lysis buffer, Triton X-100 surfactant, or DTT due to their severe
inhibition for kits’ working. We recommend using 50mM Tris+0.9%NaCL+0.1%SDS, PH7.3. You can prepare by
yourself or contact us for purchasing.

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4. Cell Culture Supernatant

Collect the supernatant: Centrifuge at 2500 rpm at 2-8℃ for 5 minutes, then collect clarified cell culture
supernatant to detect immediately. Or you can aliquot the supernatant and store it at -80°C for future’s
assay.
5. Cell Lysate

5.1. Suspension Cell Lysate: Centrifuge at 2500 rpm at 2-8℃ for 5 minutes and collect cells. Then add pre-
cooling PBS into collected cell and mix gently. Recollect cell by repeating centrifugation. Add 0.5-1ml cell
lysate and appropriate protease inhibitor (e.g. PMSF, working concentration: 1mmol/L). Lyse the cell on ice
for 30min-1h or disrupt the cell by ultrasonic disruption.
5.2. Adherent Cell Lysate: Absorb supernatant and add pre-cooling PBS to wash three times. Add 0.5-1ml cell
lysate and appropriate protease inhibitor (e.g. PMSF, working concentration: 1mmol/L). Scrape the adherent
cell with cell scraper. Lyse the cell suspension added in the centrifuge tube on ice for 30min-1h or disrupt the
cell by ultrasonic disruption.
5.3. During lysate process, use the tip for pipetting or intermittently shake the centrifugal tube to completely
lyse the protein. Mucilaginous product is DNA which can be disrupted by ultrasonic cell disruptor on ice.
(3~5mm probe, 150-300W, 3~5 s/time, 30s intervals for 1~2s working).

5.4. At the end of lysate or ultrasonic disruption, centrifuge at 10000rpm at 2-8℃ for 10 minutes. Then, the
supernatant is added into EP tube to detect immediately. Or you can aliquot the supernatant and store it at -
80°C for future’s assay.
Notes: Read notes in tissue sample.
6. Other Biological Sample

Centrifuge samples for 15 minutes at 1000×g at 2-8℃. Collect the supernatant to detect immediately. Or you
can aliquot the supernatant and store it at -80°C for future’s assay.
Recommended reagents for sample preparation: Cat No: E051 100mM PMSF protease inhibitor, Cat No:
E050 FineTest Lysis Buffer (for ELISA).

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Notes for Samples
1. Blood collection tubes should be disposable and non-endotoxin. Avoid to use hemolyzed and lipemia
samples.

2. The best sample storage condition: less than 5 days at 2-8℃; within 6 months at -20℃; within 2 years at
-80℃. Stored in liquid nitrogen for a longer storage. When melting frozen samples, rapid water bath at 15-
25℃ can decrease the effect of ice crystal (0℃) on the sample. After melting, centrifuge to remove the
precipitate, and then mix well.
3. The detection range of this kit is not equivalent to the concentration of analyze in the sample. For analyses
with higher or lower concentration, please properly dilute or concentrate the sample.
4. Pretest is recommended for special samples without reference data to validate the validity.

Precautions for Kits

1. When using different Elisa kits, labeling is required to avoid mixed components and failed assay.
2. After opening the kit, please refer to the table of storage condition for coated plate and standards
(Dampness may decrease the activity.). If any component is missing or damaged during the assay or storage,
please contact us for ordering a new one to replace.(e.g. E002 lyophilized standard)
3. Sterile and disposable tips are required during the assay. After use, the reagents bottle cap has to be
tightened to avoid the microbial contamination and evaporation.
4. While manual washing, please keep tips or pipettors for adding wash buffer away from the well.
Insufficient washing or contamination easily causes false positive and high background.
5. During the assay, prepare required reagents for next step in advance. After washing, add the reagent into
the well in time to avoid dryness. Otherwise, dry plate will result in the failed assay.
6. Before confirmation, reagents from other batches or sources should not be used in this kit.
7. Don't reuse tips and tubes to avoid cross contamination.
8. After loading, seal the plate to avoid the evaporation of the sample during incubation. Complete the
incubation process at recommended temperature.
9. Please wear the lab coat, mask and gloves to protect yourself during the assay. Especially, for the
detection of blood or other body fluid samples, please follow regulations on safety protection of biological
laboratory.

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Recommended Sample Dilution Ratio
Please refer to shipped instructions or contact us for samples, dilution as well background
info.

If other dilution ratio for your sample model is required, please refer to the universal dilution ratio below.
(The ratio is suitable for single-well assay. For duplicate assay, please follow the calculation: volume of
sample and diluent x number of duplicate well)
For 2 fold dilution (1/2): One step dilution. Add 60μL sample into 60μL sample diluent and mix gently.
For 5 fold dilution (1/5): One step dilution. Add 24μL sample into 96μL sample diluent and mix gently.
For 10 fold dilution (1/10): One step dilution. Add 12μL sample into 108μL sample diluent and mix gently.
For 20 fold dilution (1/20): One step dilution. Add 6μL sample into 114μL sample diluent and mix gently.
For 50 fold dilution (1/50): One step dilution. Add 3μL sample and 47μL normal saline (0.9% NaCl) into 100
μL sample diluent and mix gently.
For 100 fold dilution (1/100): One step dilution. Add 3μL sample and 177μL normal saline into 120μL sample
diluent and mix gently.
For 1000 fold dilution (1/1000): Two step dilution. Create a 50-fold dilution first (normal saline is used
throughout the dilution). Then, create a 20-fold dilution and mix gently.
For 10000 fold dilution (1/10000): Two step dilution. Create a 100-fold dilution first (normal saline is used
throughout the dilution). Then, create the same dilution again and mix gently.
For 100000 fold dilution (1/100000): Three step dilution. Create a 50-fold dilution and 20-fold dilution
respectively (normal saline is used in the first two steps.) Finally, create a 100-fold dilution and mix gently.

Notes: The volume in each dilution is not less than 3μL. Dilution factor should be within 100 fold. Mixing
during dilution is required to avoid foaming.

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Reagent Preparation and Storage

Take the Elisa kit from the fridge around 20 minutes earlier and equilibrate to room temperature(18-25℃).
For repeated assays, please just take the strips and standards required for the current assay, store the rest
materials according to the relevant condition.

1. Wash Buffer
Dilute 30ml (15ml for 48T) concentrated wash buffer to 750ml (375ml for 48T) wash buffer with deionized or
distilled water and mix well. (The recommended resistivity of ultrapure water is 18MΩ.) Alternatively, take
appropriate amount of concentrated wash buffer according to the assay requirement, then create a 25-fold
dilution and mix well. Store the rest solution at 2-8℃.

Crystals formed in the concentrated wash buffer can be heated by water bath at 40℃ till complete
dissolution. (Heating temperature should be below 50℃.) Mix well for the next step. It's better to use up the
prepared wash buffer in one day. Store the rest buffer at 2-8℃ within 48h.

2. Standards
2.1. Centrifuge standards tube for 1min at 10000xg. Label it as Zero tube.
2.2. Add 1ml sample dilution buffer into the standard tube. Tighten the tube cap and Let it stand for 2min at
room temperature. Invert the tube several times to mix gently. (Or you can mix it using a low speed vortex
mixer for 3-5 seconds.)
2.3. Centrifuge the tubes for 1min at 1000xg, making the liquid towards the bottom of tube and removing
possible bubbles.
2.4. Standard dilution: Label 7 EP tubes with 1/2, 1/4, 1/8, 1/16, 1/32, 1/64 and blank respectively. Add
0.3ml of the sample dilution buffer into each tube. Add 0.3ml solution from zero tube into 1/2 tube and mix
them thoroughly. Transfer 0.3ml from 1/2 tube into 1/4 tube and mix them thoroughly. Transfer 0.3ml from
1/4 tube into 1/8 tube and mix them thoroughly, so on till 1/64 tube. Now blank tube only contain 0.3ml
sample dilution buffer. The standard concentration from zero tube to blank tube is 4000ng/ml, 2000ng/ml,
1000ng/ml, 500ng/ml, 250ng/ml, 125ng/ml, 62.5ng/ml, 0ng/ml.

Notes: Store the zero tube with dissolved standards at 2-8℃ and use it within 12h. Other diluted working
solutions containing standards should be used in 2h.

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3. Preparation of Biotin-labeled Antibody Working Solution
The working solution should be prepared within 30min before the assay and can't be stored for a long time.
3.1. Calculate required total volume of the working solution: 50ul/well x quantity of wells. (It's better to
prepare additional 100ul-200ul.)
3.2. Centrifuge for 1min at 1000xg in low speed and bring down the concentrated biotin-labeled antibody to
the bottom of tube.
3.3. Dilute the biotinylated detection antibody with antibody dilution buffer at 1/100 and mix them
thoroughly. (e.g. Add 10ul concentrated biotin-labeled antibody into 990ul antibody dilution buffer.)

4. Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution

The working solution should be prepared within 30min before the assay and can't be stored for a long time.
4.1. Calculate required total volume of the working solution: 100ul/well x quantity of wells. (It's better to
prepare additional 100ul-200ul.)
4.2. Centrifuge for 1min at 1000xg in low speed and bring down the concentrated SABC to the bottom of
tube.
4.3. Dilute the concentrated SABC with SABC dilution buffer at 1/100 and mix them thoroughly. (e.g. Add
10ul concentrated SABC into 990ul SABC dilution buffer.)

Assay Procedure Summary

Step1: Wash plate 2 times before adding Standard, Sample and Control (blank) wells!
Step2: Add 50ul Standard or Sample into each well. Immediately add 50ul Biotin-labeled Antibody into each
well, gently tap the plate for 1min to ensure thorough mixing then static incubate for 45 minutes at 37°C.
Washing: Wash the plate three times and immerse for 1min each time.
Step 3: Add 100ul SABC working solution into each well, seal the plate and static incubate for 30 minutes at
37°C.
Washing: Wash the plate five times and immerse for 1min each time.
Step 4: Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C.
(Accurate TMB visualization control is required.)
Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.

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Detailed Assay Procedure
When diluting samples and reagents, they must be mixed completely. It's recommended to plot a standard
curve for each test.
1. Set standard, pilot samples, control (blank) wells on the pre-coated plate respectively, and then, records
their positions. It's recommended to measure each standard and sample in duplicate to decrease
experimental errors. Wash plate 2 times before adding standard, sample and control (blank) wells!
2. Standards and samples loading: Aliquot 50ul of zero tube, 1st tube, 2nd tube, 3 rd tube, 4th tube into each
standard well. Also add 50ul sample dilution buffer into the control (blank) well. Then, add 50ul pilot
samples into each sample well. Immediately add 50ul Biotin-labeled Antibody Working Solution into each
well, gently tap the plate for 1min to ensure thorough mixing then static incubate for 45 minutes at 37°C.
(Please keep tips or pipettors for adding Biotin-labeled Antibody away from the liquid level.)
3. Wash three times: Remove the cover, then absorb the liquid in the plate or tap the plate on a clean
absorbent paper two or three times. Add 350ul wash buffer into each well and immerse for 1min. Discard
the liquid in the well and tap on the absorbent paper again. Repeat the washing step three times.
4. HRP-Streptavidin Conjugate (SABC): Add 100ul SABC working solution into each well. Seal the plate and
static incubate for 30 minutes at 37°C. (Put the whole bottle of TMB into the 37°C incubator to equilibrate)
5. Wash five times: Remove the cover, and then wash the plate with wash buffer five times. Read washing
method in step 3.
6. TMB Substrate: Add 90ul TMB Substrate into each well, seal the plate and static incubate at 37°C in dark
within 10-20 minutes. Run the microplate reader and preheat for 15min.
(Notes: Please do not use the reagent reservoirs used by HRP couplings. The reaction time can be shortened
or extended according to the actual color change, but not more than 30 minutes. You can terminate the
reaction when apparent gradient appeared in standard wells. Weaker or stronger color intensity is
unacceptable. Please refer to TMB color rendering control in page 2 or QR code for detail.)
7. Stop: Keep the liquid in the well after staining. Add 50ul stop solution into each well. The color will turn
yellow immediately. The order for adding stop solution and TMB substrate solution is the same.
8. OD Measurement: Read the O.D. absorbance at 450nm in a microplate reader immediately. (If your
microplate reader has a choice of correction wavelength, set it to 570nm or 630nm. Correct the read value
to the OD450 value minus the OD570 or OD630 value. In this way, the OD value of non-chromogenic
substances can be corrected and removed, thus obtaining more accurate results. If the microplate reader
does not have a 570nm or 630nm wavelength, the original OD450 value can be used.)

Calculation of Results
（Operate Video：https://www.fn-test.com/videos/elisa-sample-concentration-calculation/）
1. Calculate the mean OD450 value (using the original OD450 value or the corrected OD450 value) of the
duplicate readings for each standard, control, and sample.
2. Create a four parameter logistic curve by plotting the mean absorbance for each standard on the y-axis
against the concentration on the x-axis. Alternatively, you can use the curve fitting software offered by the
microplate reader (e.g. Thermo SkanIt RE software, Curve Expert 1.3 or 1.4 available in FineTest website).
3. Calculate the sample concentration by substituting OD450 value into the standard curve. Diluted samples
should be multiplied by the relevant dilution ratio.

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Typical Data & Standard Curve
The following assay data are provided for reference, since experimental environment and operation are
different. The establishment of standard curve depends on your own assay.

STD.(ng/ml) OD-1 OD-2 Average

0 2.206 2.27 2.238

62.5 1.634 1.682 1.658

125 1.403 1.443 1.423

250 1.016 1.046 1.031

500 0.656 0.676 0.666

1000 0.448 0.46 0.454

2000 0.305 0.313 0.309

4000 0.176 0.182 0.179

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Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three
different plates.

Item Intra-assay Precision Inter-assay Precision

Sample 1 2 3 1 2 3

n 20 20 20 20 20 20

Mean (ng/ml) 120.2 485.6 2012 124.6 494.3 1993

Standard
5.7 23.65 98.99 6.17 23.97 99.25
deviation

CV(%) 4.74 4.87 4.92 4.95 4.85 4.98

Recovery
Add a certain amount of SA into the sample. Calculate the recovery by comparing the measured value with
the expected amount of SA in the sample.

Matrix Recovery Range (%) Average (%)

Serum(n=10) 89-100 97

EDTA Plasma(n=10) 86-103 91

Heparin Plasma(n=10) 87-102 91

Linearity
Dilute the sample with a certain amount of SA at 1:2, 1:4 and 1:8 to get the recovery range.

Matrix 1:2 1:4 1:8

Serum(n=10) 85-105% 92-105% 86-98%

EDTA Plasma(n=10) 85-99% 82-100% 82-98%

Heparin Plasma(n=10) 82-98% 85-96% 83-95%

Stability
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Elisa kit(n=5) 37°C for 1 month 2-8°C for 6 months

Average (%) 80 95-100

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ELISA Troubleshooting
If the ELISA result is unsatisfied, please take a screenshot for the staining result and store the OD data. Keep
used strips as well the rest reagents. Contact us to solve your problem with the kit’s catalogue number and
batch number. You can also refer to the following table to check the reason.

Problem Possible Causes Solutions

Confirm the required reagent added in each

Incorrect order for adding reagents
step. Also repeat the assay and verify.
Standard curve
Use the component included in the same kit.
without signal Use components from different kits
Also repeat the assay and verify.

Forget to add some reagents Verify whether the required reagent is added.

Use components from different kits,

Use the component included in the same kit.
Overflow OD or prepare the working solution with
Also repeat the assay and verify.
higher concentration

Poor standard Try to plot the curve by different fitting

Inappropriate curve fitting model
curve models.

The amount of pilot sample is lower Decrease dilution ratio or concentrate the
than the detection range. sample.

The detection target is incompatible Verify the compatibility of sample storage

Samples without with the buffer. buffer with the pilot sample.
signal Please refer to sample preparation guideline
Incorrect preparation of sample
and regularly store.

Longer storage of sample or freeze- Aliquot and store samples according to the
thaw cycle assay requirement.

Precipitate is formed in the well

Increase the dilution ratio of the sample.
during staining.

Don't touch the bottom of the plate during the

Unclean plate
assay.

Avoid foaming during reading in a microplate

Foam is found in the well.
High CV% reader.

Check whether the tube of the washer is

Each well is washed unevenly.
smooth.

Reagents are not completely mixed. Mix all reagents completely.

Use calibrated pipette and correct pipetting

Inconsistent pipetting
method.

Before opening, shortly centrifuge the

Standards are improperly
lyophilized standard tube till complete
Standard curve reconstituted.
dissolution.
with low signal
Standards have been degraded. Follow suggested storage conditions for

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 standards.

When pipetting, the required volume Use calibrated pipette and correct pipetting
is incorrect or inaccurate. method.

Expired kit Don't use expired products.

Follow suggested storage conditions for all

Improper storage
components.

The assay and sample loading process can't be

terminated. Especially after washing the plate,
The well is over dried.
add reagents immediately. Seal the plate
during incubation.

Before use, equilibrate the whole bottle of

Slow colorimetric reaction TMB substrate for 30min at 37°C. Extend the
incubation time.

The wavelength of the microplate Check the wavelength and read the OD450
reader is incorrect. value again.

The well is washed excessively. Follow suggested washing times in this manual.

Insufficient washing Follow suggested washing times in this manual.

Use the prepared wash buffer immediately.

Wash buffer is contaminated. During manual washing, add wash buffer
without touching the well.

Too many detection reagents or Use calibrated pipette and correct pipetting
High Background
higher concentration. method.

Read the assay result immediately after adding

Reading of assay result is not in time.
the stop solution.

TMB substrate is incubated in strong

During colorimetry, incubate in the dark.
light.

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Declaration
1. Limited to current conditions and scientific techniques, all raw materials are not completely identified and
analyzed. This product may have a technology-related quality risk.
2. During the Elisa kit development, some endogenous interferons(not all) in the biological sample have been
removed or decreased.
3. The final assay result is related to the validity of reagents, experimental operation and environment.
FineTest is only responsible for this kit, excluding sample consumption during using this kit. Before use,
please consider and prepare enough samples required by the assay.
4. To get a satisfied assay result, please use all reagents offered by this kit. Don't use any product from other
vendors. Strictly follow instructions of this manual.
5. During assay procedure, incorrect reagents preparation and parameter setting of the microplate reader
may result in the abnormal result. Before assay, please read this manual carefully and adjust instruments.
6. Even if the assay is performed by the same person, results in two independent assays may be different.
Thus, each step in the assay should be controlled to ensure the reproducibility.
7. Before delivery, this kit is subject to the strict QC. Influenced by transportation conditions and
experimental devices, the assay result got by the customer may be different from original data. Inter-assay
CV between different batches may be caused by reasons before.
8. This kit is not compared to similar kits from other vendors or methods for testing the same detection
target. Thus, assay results may be inconsistent.
9. This kit allows for research use only. For IVD or other purposes, FineTest is not responsible for relevant
consequences and doesn't bear any legal liability.

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