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Biochemistry and Clinical Pathology Practical

The document appears to be a laboratory manual detailing various experiments related to the identification and analysis of carbohydrates and unknown samples. It includes procedures for urine analysis, buffer preparation, and tests for different types of sugars. The document is structured with sections outlining the introduction, methods, and specific tests, but contains numerous typographical errors and unclear formatting.

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0% found this document useful (0 votes)
26 views41 pages

Biochemistry and Clinical Pathology Practical

The document appears to be a laboratory manual detailing various experiments related to the identification and analysis of carbohydrates and unknown samples. It includes procedures for urine analysis, buffer preparation, and tests for different types of sugars. The document is structured with sections outlining the introduction, methods, and specific tests, but contains numerous typographical errors and unclear formatting.

Uploaded by

nhazra430
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Pe «el INDEX Name of the Experiment Page Decent Ly» Date of ay = Remarks 4 jy. | Brtreduetion. amd Handling Govan 04-03 | | General teaclion of Gxbobuydrate. 04. | Tdentification of Unleno eT jentif & Unknown Somple: | Tienbifieabion of Unlenoion Somple- &, | Luentficabion f Unknown Sorple-B | Lientificabion of Unknaom Saple- Antroduction 4 Lpids H. | Lantifeakion of Unieown Somple -L |It-8 | | Barfiekion A Unkvown Somple-® | 4-22 Be] Urine Anodysis _ Phipiced eparminobion| 23-30 | Analyoe de given umine, Somple, for ts hepmel Const tuont - 5 Probie de given Urine Some for its hotemel Const tuank — IT Freporosion of buffer ~ 33 Selition omd wearunemanh He, PH volo f tha. Seustion | [->— ° PIONEER’ E ee (ou ETROOUETION. AND HanDLIaG OF GLAssinnees fe | sn Ta Ulan Aygo ol alomsoares, uss fn Biochemieha= Lalo. ee oan On 2004 Page. no, (32-33 1 i eaouting CAlindor | ae dy. See foal. | | 2 Conical Alok 47. Pipatto, a 6 Glam oc. | V ede eesti aise ® Euvnel aur Thong 9). 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EE | : 1 Movteramd Pestle, Couctblerand | Funal Coven Coucible songs ——{] “Test due holden Plattoren balance, Ctriple. beam) Fos one. ghovsia0ie be ornodnerr alos Lois» i i fies a Safe PIONEER” Page: OU, ae pes One ad | Trane, ote, Hae Sinaplent ree frites sale gen Hapaaie Seale Sai trator ond ow £ Boned. ss dunctional yup. | Tynes ot tao type — 2 Aldnse. ¥> kedose.. T — Mea © PICHEER Page: OF Dee: ff ~ : | | Lp Aloose > [hem the fumedional areups in Manasaceharises t¢ | __aldelyle: the dharsoe. called or Aldose 2a by cose. Ler daonse te A Motose, > (dhoen. dhe. furetiomal pocups fn Menesacclasile. ts lotone Haye one calles om kedore.. eo - Ribuloee, , Fructose ete ar | “BE thigncacchaviden Those. cotay totes cabins 2ai0 wd | senna ale ae eee fm somes |_wides _foased on the number 6h trnono soe fraxides — —Disrechadiides. these ates tet. Gnlonlydeake tole. contains — j—Dueoleccslar ef some on diffomak dyer df natenincclorties | —= reese, Glucete + Frucdnee, | La tose, —bdenlesis—s Glucose + Gilactoge,. | Moadlnvo, —wyarlesis—> Glucose, + Cilcose . |_® op, ides 3- Thew eh fells nae Han Osan | mabwion ond fingolulole tn waber i IL Bol gncetaonst ‘des aim fustia divided nln Big — | 22. Hamopaly sacehonides — These. J tes wpa | ba veo vuseler sh saemee wenn sacelovile | Putte, — Stonol »— roe aces ‘des — 4 Aiase_ate_dut ovis dates Obiel. peoduces| [different tnnniertacelari oe eae KH de ou ! w—c—oH ae let Cone. 4aS04, \ 3H,0 H— ¢—oH \ H— c— 6H | CH 98H 6 \ e— 4 I < \\ Hoe | O H— Cc {I a { CH) OH Hydromy meting! ty tusidural h—wapthol Violet Coloured Coen pound e PIOREER — Page: Ob» Dee: Jf Ee { LARGOWYDRATES spes02 | [LM tot TE io gna at alt Lae aE ee eT aren NTT ee tls d= maptral by foern colouried propdueds IF pape ferredieea in: 2-% dwops of Bh Snape main oud add Dal of Cone. HyS0u along Hie cides onal desk babes» Sn ee er a folet votn: wer ok Moe juncHow sb 2 Unidas Hao ih indicat WOE ek ke te oda | Reagent — 6 d= bopilel male Cone. HySb4 + _ 2 Toi. det aaa =a eee eae fon Ai fs ne ee [tnd gives cll Padine., Brculepec Morn 1s insolutale. fa tonter ond fovinws gel which gives vinlat ere brawn ¢olntss | en caer aaa Hao, | — ae a Reaction. ugar Comdial Seaniac tingdy et gy eet Capes tone] Cot Nak Od + Cag) gf ie COM] Cuprous onide, ierlas tipectde. Chea’) ‘ Cyelow) \ Ee a TE aan ene ‘ep plonere® page: OF - = er tans beeInieases Lut. caloun takers omilaso. solution fs | __teaded the holical tonbinnn ation is dinduched ond it bie do Kind fodine. Bin cocking dhe. oniginal | 1 eHow fe iq 5 ’ ee | Boeeduunes = Take 20net Sawple clue, add 2% dens ob tn. Audion 1 if bolus. enlowr appeasic , tt trdicater the | Or presenco, sf prbysocebositdes . te. - sbovieh « | 4 thee. of Hy0 1] L I- ela Bs Penodict/e dest t- Thin dost te al sas ed cing | ugar. “Te cotilonbspdieabes lave. 2. | fs frown encdiol pe. Wnstolle. omc. decrmplise. | ae yi ld that ¢ ain, aldehyde. Which ote. paiierfil Peduitivgs | —a4enls Wi, con veduer, cubricion do cupnowsion which Efe 2 » for lmvedicts veortow .Denediets Solution | faindnin widens alt tolpid.. .e— Nay COs, . Sa ees” faut am a ek re ——_____ PionecR® ne Page: 08+ Date: pe CProcedutt= “Take L-2l & sowple. salulion Add Bal | |__bemediods we aenk «ain td and tootl fhe Dnata fin wabewe bakb.| dd_soporiodely. Cusoy , Solult Lata wt eae Sodiun sien eee LisaocloKides,. - Se | in. oldebyda. ee op ‘i = “Take erie pat sete oelio 1Ge 21 lee | ae " pach Oued eb folate 8 eapeinle, Mix | Dann te a cane LTE ved colour o pecans indicates the presence. of sugat = Solution. of Cus0y Se anne —Febings AB ¢ = Solukion ef Sodiunn , Patassinin dortoiate | ea pare CAH \ c=0 | OH—C—H uH—C— OH | H-—C— OH CH) OH hoe 6 Hy doom meta! dunt f Pesere} nol Red - edoux - comple IONEER” cad Page: 04, Date: 1 | R ol | 2itcal of Semple. Solution 161 Adal Diol ef Ino fa cd's col Wo lation, abso tf E Es bottom of Ho tort dubo » Reagent — Solution ot Copper aceimbe tn qlactal acchie | Be etd, - i PIONEER al aoe ema feat to {ample sian $$ fasted naa — ee a forrmolion ecuurs _Coolerlleaten. | Gone. tyS04 foomn a | Sider of dost dube,, | | + heat a | Tadine ton i Dateien fee th | Gowple, sslution + Pai Socchart ds. | Ce Bona ‘aa Page: 12 Dae: ff ( -_—Trenteiearion of Ununoon Sauore-2 | | acest Sosenvation. a Gy fo peneo Je Moligeh dost a | Souple Solution + Psiple. nied color Prereince. ot | | Moticeh veogenk+ Come. tyihy — firmation beloenn. Cewlonligeloator, | [ fanne Sien ah do Iube,, dso layers | 2: Todine. test ei | shoveple. Sele Sodine, ln enlous Ab ternee oh [dels tisses on ai lcaal iigeahe your —_Peducivga sugar. | | 3. Bonediets Azat Goes eee No ee Absence ele | ta i - |4. Felnlinng test {| json ban ne No cal pw fuera —Fehting. naagant Heobig —dbenetiem. —— —_ttedusinge Sugars | F Setisinalt dork —Someple, Sabation + doliuaind Kod. colowde Coesenee ot- Heoaent + heat in vate bab. frorralion.- Ketone, ai: BIOMEER™ | woe Page: 13. | ee (lt wai Glucose. o a é eaia|=a ( _-TReNTIELcATION of UY Us ieeena 4 [4 Fahl tek tng +hiok on eae a cl goto bedaingaSgt bolton of deat debe | | i eee | | souple, Ylaliont Carrfoed!s reagent? Fed tees omit Cessnce of ees aes ing ee ook | + heat ov Lucho No colowe frrcabiotn Bresemee of- Uae Solativr— __dletoge. Sgt — The giver unknown gaovple 16 aldose, Sugar. Cérlueese.) Frusedo see Pisani PIONEER Page: 1B» Date: ff folubiont Maliscl _Alowple sed ing Beteabine, veagenk + + Cone. Hyhdy ——behwenn 7 4 t ae _ piesa |_daveple Solutiont Bonediel’, Crick sod Coloun pet is Presence [fest heat cabo _hoerned . reducing Sogo [3 Ebling! test | Souskion + Fetnlis - Brick sed doloun _ Prerance of i |_t eat on abenbadi, —_—_¢e forte reducrge suger. | 4, Paxdoed's dost (champ fouiont Ganfeeds reat Cena boalion —Preiac BE |_theat on worker bale ah Mel fe rein | Hl ha \ LA. Seliwhnalf tere ope Slatin alsin Peal Brick sted colour $s one | teak an wedern bel _____cfiremredl _Kerfore, cago. &. en : — Sample. Solation + Taline Sslubinn Aeon Agence of | formation Gy socchossides. | — | Bent ims eal a ene —] | tne ela, $$ —|

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