Sto.

Niño College of Science and Technology,

Incorporated
Bonifacio Street, 8100 Tagum City, Davao Del Norte, Philippines
Tel. Nos. (084) 655-6623 Email: sncst1999@yahoo.com
Elem. Government Recognition (R-XI) No. 006 s.2005; JHS Government Recognition (R-XI) No. 06, s.2005; SHS Government Permit (R-XI) No. 116 s.2017

SCIENCE LABORATORY
MANUAL

Prepared by: Christine C. Pueblas

TABLE OF CONTENTS
 INTRODUCTION TO THE SCIENCE LAB AND LABORATORY SAFETY

INTRODUCTION
The biology laboratory provides an opportunity to observe first-hand things you have studied in class. Here you
will play the role of student/scientist, learning skills and techniques that will allow you to examine biological
phenomena. During this first laboratory experience, you will be introduced to the organization of the lab, lab
equipment, lab safety rules, and become acquainted with the Biology Department’s expectations.

STUDENT OBJECTIVES
1. Become familiar with seating, material distribution, and other general lab procedures.
2. Learn general safety rules.

PROCEDURE
I. Basic Laboratory Materials, Supplies, and Equipment
The following basic materials, supplies, and equipment are showed in front on the teacher’s desk and somewhere
else in this room:
1. Containers and bottles
Beaker, Erlenmeyer flask, Florence flask, evaporating dish, finger bowl, battery jar, dropping bottle, narrow
mouth bottle, wide mouth bottle.
2. Measuring tools:
Graduated cylinder, serological pipette, volumetric pipette, micropipette with tip, rubber pipette bulb,
graduated beaker, graduated Erlenmeyer flask, ruler, triple beam balance.
3. Materials for supporting or holding
Support stand, burette clamp, support ring, tripod, test-tube rack, test-tube holder, clamp holder, tong.
4. Materials for heating
Bunsen burner, flint lighter, wing top, spot plate, alcohol lamp, wire gauze.
5. Dissection materials
Dissecting pan, scissors, probe, scalpel, dissecting needles, forceps.
6. Micro-examination materials
Compound microscope, stereoscope, slide, cover slip, hand lens, watch glass.
7. Materials for inoculating
Inoculating loop, petri dish.
8. Other general materials
Centigrade thermometer, funnel, pipette dropper, spot plate, centrifuge.
9. Safety equipment
Eyewash bottle or eye bath, safety goggle, first-aid kit, fire extinguisher.

II. General Safety Rules

1. Before you start
a. Be familiar with the working equipment. Learn the location of the fire blankets, first aid kit, eye wash
station (if available), safety shower, and fire extinguisher.
b. Check what materials and equipment the teacher has offered and learn how to handle them.
c. Be sure to tie back long hair and secure loose clothing so that they do not get caught in a Bunsen burner
flame or chemicals.
d. Understand that the laboratory is a dangerous place for fooling around or “Horse Play” of any kind.
2. While you are working
a. Perform only laboratories assigned by your teacher.
b. Pay close attention to any CAUTIONS described in the laboratory exercise.
c. Use the safety equipment, such as safety goggles and laboratory aprons, provided for you.
d. When heating substances in a test tube or other container, do not look directly into the container.
e. Be sure that the opening of the test tube or other container being used is pointed way from you and other
people.
f. Use only the chemicals listed for each laboratory.
g. To prevent contamination, never return unused chemicals to the original container.
h. Properly mount the specimen on the dissecting tray before making an incision.
i. Keep pathways clear by placing extra items such as coats, books, and purses in their designated areas. A
clean work area is the first step in preventing accidents.
j. Absolutely NO eating or drinking in the laboratory.
k. You must not insert anything other than the appropriate electrical plug in any electrical outlet. This action
will be followed by immediate Dean’s referral.
3. Having an emergency
a. Report all accidents to your teacher immediately.
b. If you spill acid, base, or other corrosive materials on yourself, immediately flush the with plenty of water.
c. Place broken glass and other solid waste materials in containers designated for their disposal.
4. When you finish
a. When you finish the laboratory, be sure to turn off the gas and water, disconnect electrical connections,
place all materials and laboratory equipment to their proper places, clean and dry any laboratory
glassware and articles, and clean your work area.
b. No specimen or material is to be taken out of the laboratory.
c. Only liquids are to be emptied into the sinks in the laboratory. No other material is to be placed into the
sinks.
 Standard Operating Procedures in Science Laboratory

A. General Safety Laboratory Procedures

The following are the Do’s and Don’ts inside the SNCST Science Laboratory:

1. Do’s
1.1. Know the nearest location of fire extinguisher, first aid kit, fire exit, hand washing
station, fire alarm, clinic, emergency call numbers and telephones for prevention and
possible accidents inside the science laboratory.
1.2. Know the possible hazards for each chemicals and materials inside the laboratory.
1.3. Wear appropriate attire when working with chemicals. This includes laboratory gown,
leather shoes, laboratory gloves, safety goggles and disposable mask.
1.4. Always prepare a personal medical kit in working with hazardous chemicals and
experiments inside the laboratory.
1.5. Observe cleanliness and work in a systematized way when doing an experiment
inside to avoid any possible accidents and unfortunate outcomes.
1.6. Wash the skin or eyes with running water if contacted by any chemicals regardless
of its toxicity and corrosivity.
1.7. Long hairs should be restrained to avoid accidents such as fire and injuries when
doing an experiment. The experimenter can wear hairnet to restrain the hair.
1.8. Return and store the chemicals properly. Label all the chemicals to identify the
containers content.
1.9. Label all new chemical containers with date received and date opened.
1.10. Always turn off the Bunsen burner or any source of heat after using to avoid fire
incident.
1.11. If the experiment includes mixing of acid, it should be carefully added to the water or
any solvent based on the experiment.
1.12. Any laboratory apparatus and chemicals should be properly handled to avoid
damage and potential accidents.

2. Don’ts
2.1. Eating, drinking, applying cosmetics or bringing any foods and drinks inside the
laboratory.
2.2. Wearing inappropriate attire without protection such as civilian, casual, formal or
only school uniform when dealing with chemicals.
2.3. Wearing half covered footwear such as sandals, slippers and rubber shoes.
2.4. Ignoring any minor or major accidents inside the science laboratory.
2.5. Drinking water coming from the laboratory water sources.
2.6. Direct smelling, tasting, touching and direct looking at any chemicals without
protection regardless with its corrosivity and toxicity.
2.7. Using laboratory apparatus as tool to prepare and consuming food.
2.8. Leaving hazardous experiments such as flammable reaction that can lead to major
incidents.
2.9. Leaving laboratory apparatus and chemicals on the experiment desk without putting
it back to the storage cabinet.
2.10. Letting any electrical-powered tools, laboratory water source and heat source active
after the experiment.

2.11. Borrowing laboratory apparatus, using chemicals and working inside the science
laboratory without the approval of Subject Teacher and Science Laboratory In-
charge.
2.12. Returning used chemicals back to their container.
2.13. Using defected and broken laboratory apparatus and expired chemicals or any other
disposable materials.

B. Rules and Regulations

1. All laboratory equipment and chemicals broke by any student during experiment
shall replace by all students in the section involved.
2. Students are not allowed to enter inside the science laboratory without approval by
the science laboratory in-charge and any faculty staff.
3. The facility is available to all grade levels every Monday to Friday (7:30 a.m. to 4:05
p.m.).
4. Teachers and students who want to have a schedule for using the science
laboratory must inform the lab in-charge within 3-7 days before the day of using the
facility.
5. The science laboratory is only available for conducting experiments and other
science-related activities. However, it can be used for substitute facility such as
conference room if approved by the science lab. In-charge.
6. Students who used the science laboratory are required to clean the facility after the
activity.
7. Best science projects/outputs can be displayed in the science laboratory.

C. Implementation
1. Standard operating procedures and guidelines mentioned above shall be effective at
the time it is signed by the school head and science laboratory in-charge.

Prepared by:

CHRISTINE C. PUEBLAS
Science Laboratory In-charge

Approved by:

VETERVA L. PERLA, Ph D.
School Principal

Noted by:

BEATRIZ P. NAMUAG
School Administrator