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    Proteus Mirabilis

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    JaveriaZafar
    The document describes a study that identified the idsABCDEF gene locus in Proteus mirabilis as important for self-nonself discrimination and boundary formation between bacterial strains. The ids gene cluster was found to be conserved in other P. mirabilis strains. Through transposon mutagenesis and screening mutants, genes idsB, idsC, idsD, idsE and idsF were determined to play a role in self recognition, with idsD and idsE encoding specific molecular identifiers. A model is proposed where idsD and idsE possess strain-dependent variable domains that allow for self identification.

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    Proteus Mirabilis

    Uploaded by

    JaveriaZafar
    119 views18 pages
    The document describes a study that identified the idsABCDEF gene locus in Proteus mirabilis as important for self-nonself discrimination and boundary formation between bacterial strains. The ids gene cluster was found to be conserved in other P. mirabilis strains. Through transposon mutagenesis and screening mutants, genes idsB, idsC, idsD, idsE and idsF were determined to play a role in self recognition, with idsD and idsE encoding specific molecular identifiers. A model is proposed where idsD and idsE possess strain-dependent variable domains that allow for self identification.

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    proteus mirabilis

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    The document describes a study that identified the idsABCDEF gene locus in Proteus mirabilis as important for self-nonself discrimination and boundary formation between bacterial strains. The ids gene cluster was found to be conserved in other P. mirabilis strains. Through transposon mutagenesis and screening mutants, genes idsB, idsC, idsD, idsE and idsF were determined to play a role in self recognition, with idsD and idsE encoding specific molecular identifiers. A model is proposed where idsD and idsE possess strain-dependent variable domains that allow for self identification.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    119 views18 pages

    Proteus Mirabilis

    Uploaded by

    JaveriaZafar
    The document describes a study that identified the idsABCDEF gene locus in Proteus mirabilis as important for self-nonself discrimination and boundary formation between bacterial strains. The ids gene cluster was found to be conserved in other P. mirabilis strains. Through transposon mutagenesis and screening mutants, genes idsB, idsC, idsD, idsE and idsF were determined to play a role in self recognition, with idsD and idsE encoding specific molecular identifiers. A model is proposed where idsD and idsE possess strain-dependent variable domains that allow for self identification.

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    You are on page 1of 18

    _________________________________________________________

    _________________________________________________________________________

    Proteus mirabilis
    gram negative, facultative anaerobe rod
    causes infections in human urinary tract
    (pyelonephritis, kidney stones) followed by
    septicaemia
    motile bacterium with peritrichous flagella
    Morphology change in swarming process

    _________________________________________________________________________

    Swarming proteus mirabilis


    bulls-eye swarming on solid surfaces & self
    identity

    _________________________________________________________________________

    Boundary between P. mirabilis strain


    BB2000 and HI4320

    _________________________________________________________________________

    Aim of study
    Identification and characterization of
    the genetic mechanism that is
    responsible for bacterial self identity

    _________________________________________________________________________

    Construction of p. mirabilis
    mutant library
    randomized Tn5-transposon mutagenesis of
    rifampicin resistent p. miarabilis BB2000
    Tn5-cat

    Shuttle vector

    cat

    conjugal transfer by mating of E.coli [pUTminiTn5Cm] and BB2000

    _________________________________________________________________________
    tnp: Transposase; mobRP4: conjugal transfer domain

    Loss of self recognition I


    Screening of 3600 transposon BB2000 mutants
    revealed one mutant with phenotypic loss of self
    recognition
    identification of self (Ids)

    _________________________________________________________________________

    Loss of self recognition II


    six gene cluster idsABCDEF carries information for
    identification of self

    _________________________________________________________________________

    Phenotyping of individual
    idsABCDEF knock outs

    idsB idsF are required for self recognition !


    _________________________________________________________________________

    Phenotyping of individual
    idsABCDEF knock outs in
    HI4320 background

    idsB,
    idsD and
    idsCidsE
    and idsF
    are essential
    can be complemented
    for self recognition
    by allels
    ! of different
    _________________________________________________________________________

    idsD and idsE encode identity


    determinants

    .but the ids gene cluster is not the sole determinant of boundary
    ation !

    boundary between BB2000+pidsHI4320 and HI4320+pidsBB2000


    _________________________________________________________________________

    Conserved organization of ids


    gene cluster in other P.
    mirabilis isolates

    idsABCDEF with nearly identical length and ~96% identity to homolog

    idsD and idsE have variable domains in protein sequences


    _________________________________________________________________________

    Additional putative idsE genes


    in genome of P. mirabilis
    HI4320

    _________________________________________________________________________

    Model of self recognitation

    idsA is not required for self identity; idsF overtakes function distinct from

    dsB
    _________________________________________________________________________
    and idsC in self-nonself recognition; idsD and idsE are molecular self
    dentifiers

    Summary
    gene locus idsABCDEF of P. mirabilis is important for
    self-nonself discrimination and boundary formation
    Conserved ids gene cluster has been found in other
    proteus mirabilis strains
    idsA is not required for parental recognition
    idsB, idsC and idsF are interchangeable between
    strains
    idsD and idsE are specific molecular identifiers that
    possess strain dependent variable domains
    other ids homologs in bacterial genome may
    contribute to the self recognition process

    _________________________________________________________________________

    Thank you for your attention

    _________________________________________________________________________

    P. mirabilis boundaries

    _________________________________________________________________________

    Transposition and pBBR-MCS2


    shuttle vector for mutagenesis

    _________________________________________________________________________

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