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    RH Grouping: James Pretencio Kate Febrian Dabuet Rey Angelo Z. Hidalgo Victor Francis A. Salvana

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    Marj Mendez
    The document discusses Rh grouping which involves detecting the presence of Rh antigens like D, C, c, E, and e on red blood cells through testing samples with corresponding anti-sera, as detecting these antigens is important to avoid antibody production and transfusion reactions in individuals lacking a particular antigen. The procedure outlined tests an unknown blood sample for the 5 major Rh antigens through mixing the sample on slides with anti-D, anti-C, anti-c, anti-E and anti-e sera and observing any agglutination reactions to determine the individual's Rh blood group.

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    RH Grouping: James Pretencio Kate Febrian Dabuet Rey Angelo Z. Hidalgo Victor Francis A. Salvana

    Uploaded by

    Marj Mendez
    193 views8 pages
    The document discusses Rh grouping which involves detecting the presence of Rh antigens like D, C, c, E, and e on red blood cells through testing samples with corresponding anti-sera, as detecting these antigens is important to avoid antibody production and transfusion reactions in individuals lacking a particular antigen. The procedure outlined tests an unknown blood sample for the 5 major Rh antigens through mixing the sample on slides with anti-D, anti-C, anti-c, anti-E and anti-e sera and observing any agglutination reactions to determine the individual's Rh blood group.

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    RH GROUPING

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    The document discusses Rh grouping which involves detecting the presence of Rh antigens like D, C, c, E, and e on red blood cells through testing samples with corresponding anti-sera, as detecting these antigens is important to avoid antibody production and transfusion reactions in individuals lacking a particular antigen. The procedure outlined tests an unknown blood sample for the 5 major Rh antigens through mixing the sample on slides with anti-D, anti-C, anti-c, anti-E and anti-e sera and observing any agglutination reactions to determine the individual's Rh blood group.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    193 views8 pages

    RH Grouping: James Pretencio Kate Febrian Dabuet Rey Angelo Z. Hidalgo Victor Francis A. Salvana

    Uploaded by

    Marj Mendez
    The document discusses Rh grouping which involves detecting the presence of Rh antigens like D, C, c, E, and e on red blood cells through testing samples with corresponding anti-sera, as detecting these antigens is important to avoid antibody production and transfusion reactions in individuals lacking a particular antigen. The procedure outlined tests an unknown blood sample for the 5 major Rh antigens through mixing the sample on slides with anti-D, anti-C, anti-c, anti-E and anti-e sera and observing any agglutination reactions to determine the individual's Rh blood group.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 8

    RH

    GROUPING
    JAMES PRETENCIO
    KATE FEBRIAN DABUET
    REY ANGELO Z. HIDALGO
    VICTOR FRANCIS A. SALVANA
    RH GROUPING
    Routine Rh grouping for donor and patients involve detection of only the D antigen on
    red cells. Blood samples are Rh (D) positive or Rh (D) negative depending on the
    absence of the D antigen, respectively. However, red cell i as C, E, c, e belong to this
    second most important blood group system: Rh system. Their detection is equally
    important among individuals because if they are exposed to Rh antigens which they lack,
    this results to antibody production and subsequent transfusion of blood having the Rh
    antigens will bring about transfusion reaction. The five major Rh antigens in decreasing
    immunogenicity are as follows: D,c,E, C,e (Harmening, 2005).
    Materials Needed
     Glass slides
     Marking pen
     Lancet (with 3 mm blade)
     70% alcohol
     Gum label
     Applicator sticks
     Cotton
     Rh view box (surface temperature: 45-50°C)
    Reagents/Sample
     Anti-D serum
     Anti-C serum
     Anti-c serum
     Unknown blood sample
     Anti-E serum
     Anti-e serum
     Rh/Hr Control (CTRL)
    Procedure
    1. Prepare 2 glass slides

    2. Divide each slide into 3 portions by using a marking pen

    3. Label the 3 portions of the first slide as D, C, and c. Label the 3 portions of the as E, e, and Ctrl.

    4. Place the glass slides on Rh view box (surface temperature 45-50C) for 5 minutes

    5. Remove the pre-warmed slides and deliver 1 drop of anti-D, anti-C, Anti-E, anti-e, and Rh/Hr control on the appropriately marked portion of the

    glass slide.

    6. Perform skin puncture by prick method and add 1 drop of whole blood to each portion

    7. Mix well with separate applicator sticks.

    8.
    Observation
    Draw and color your results. Label the slides completely then interpret me determine the
    Rh group.
    Name of patient____________________________

    D C c

    Reaction __________ ___________ _________


    E e CTRL

    Reaction __________ ___________ __________


    Antigen/s Detected :
    Rh blood group
    Procedure
    1. A
    2. A
    3. A
    4. Mix gently. Cover each tube with nescofilm. Centrifuge all tubes
    for 15 seconds
    5. Gently dislodge the cell button and examine for agglutination
    6. Grade each reaction and record the results
    Lab Questions
    1. Why is it necessary to pre-warm the slide prior to its use?
    2. How is Rh typing serum prepared?
    3. Give some causes of false positive and false negative reactions?

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