Scribd Logo
Upload

Welcome to Scribd!

  • 29 views

    11 Biotechnology Principles N Processes - PPSX

    Uploaded by

    komatipallis615
    Biotechnology is defined as using live organisms or their enzymes for products and processes useful to humans. It involves techniques like genetic engineering, in vitro fertilization, DNA vaccines, and correcting defective genes. Modern biotechnology uses principles like genetic engineering and bioprocess engineering. Genetic engineering involves altering genetic material like DNA and RNA and introducing it into host organisms. Bioprocess engineering maintains sterile conditions for growing microbes or cells in large quantities to produce antibiotics, vaccines, and enzymes. Recombinant DNA technology is the process of joining foreign DNA to host DNA to produce new genetic combinations, and was first achieved by Stanley Cohen and Herbert Boyer in 1972.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    11 Biotechnology Principles N Processes - PPSX

    Uploaded by

    komatipallis615
    29 views14 pages
    Biotechnology is defined as using live organisms or their enzymes for products and processes useful to humans. It involves techniques like genetic engineering, in vitro fertilization, DNA vaccines, and correcting defective genes. Modern biotechnology uses principles like genetic engineering and bioprocess engineering. Genetic engineering involves altering genetic material like DNA and RNA and introducing it into host organisms. Bioprocess engineering maintains sterile conditions for growing microbes or cells in large quantities to produce antibiotics, vaccines, and enzymes. Recombinant DNA technology is the process of joining foreign DNA to host DNA to produce new genetic combinations, and was first achieved by Stanley Cohen and Herbert Boyer in 1972.

    Original Title

    11 Biotechnology Principles n Processes.ppsx (1)

    Copyright

    © © All Rights Reserved

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Biotechnology is defined as using live organisms or their enzymes for products and processes useful to humans. It involves techniques like genetic engineering, in vitro fertilization, DNA vaccines, and correcting defective genes. Modern biotechnology uses principles like genetic engineering and bioprocess engineering. Genetic engineering involves altering genetic material like DNA and RNA and introducing it into host organisms. Bioprocess engineering maintains sterile conditions for growing microbes or cells in large quantities to produce antibiotics, vaccines, and enzymes. Recombinant DNA technology is the process of joining foreign DNA to host DNA to produce new genetic combinations, and was first achieved by Stanley Cohen and Herbert Boyer in 1972.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    29 views14 pages

    11 Biotechnology Principles N Processes - PPSX

    Uploaded by

    komatipallis615
    Biotechnology is defined as using live organisms or their enzymes for products and processes useful to humans. It involves techniques like genetic engineering, in vitro fertilization, DNA vaccines, and correcting defective genes. Modern biotechnology uses principles like genetic engineering and bioprocess engineering. Genetic engineering involves altering genetic material like DNA and RNA and introducing it into host organisms. Bioprocess engineering maintains sterile conditions for growing microbes or cells in large quantities to produce antibiotics, vaccines, and enzymes. Recombinant DNA technology is the process of joining foreign DNA to host DNA to produce new genetic combinations, and was first achieved by Stanley Cohen and Herbert Boyer in 1972.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 14

    © www.bankofbiology.

    com
    www.bankofbiology.com
    What is Biotechnology?

    Biotechnology is the techniques of using live organisms or their


    enzymes for products and processes useful to humans.

    www.bankofbiology.com
    What is Biotechnology?

    European Federation of Biotechnology (EFB) defines


    Biotechnology as ‘the integration of natural science and
    organisms, cells, parts thereof, and molecular analogues
    for products and services’.
    www.bankofbiology.com
    What is Biotechnology?
    Microbe-mediated processes
    (making curd, bread, wine etc).

    In vitro fertilization
    (‘test-tube’ baby programme)

    Biotechnology Synthesis and using of a gene


    deals with

    Preparation of DNA vaccine

    Correcting a defective gene

    www.bankofbiology.com
    Principles of Biotechnology
    Core Techniques of Modern Biotechnology

    Genetic engineering Bioprocess engineering


    The technique in which Maintenance of sterile
    genetic material (DNA & ambience in chemical
    engineering processes for
    RNA) is chemically altered
    growing desired microbe/
    and introduced into host
    eukaryotic cell in large
    organisms to change the
    quantities for the
    phenotype. manufacture of antibiotics,
    vaccines, enzymes etc.

    www.bankofbiology.com
    Principles of Biotechnology
    Basic steps in genetically modifying an organism

    1. Identification of DNA with desirable genes

    2. Introduction of the identified DNA into the


    host

    3. Maintenance of introduced DNA in the host


    and transfer of the DNA to its progeny.
    www.bankofbiology.com
    Principles of Biotechnology
    Basic steps in genetically modifying an organism

    1. Identification of DNA with desirable genes


    • Traditional hybridisation techniques lead to
    inclusion and multiplication of undesirable
    genes & desirable genes.
    • In genetic engineering, only desirable genes
    are introduced.

    www.bankofbiology.com
    Principles of Biotechnology
    Basic steps in genetically modifying an organism

    2. Introduction of the identified


    DNA into the host
    • A vector DNA such as plasmid is used
    to deliver an alien piece of DNA into
    the host organism.

    www.bankofbiology.com
    Principles of Biotechnology
    Basic steps in genetically modifying an organism

    3. Maintenance of introduced DNA in the host and


    transfer of the DNA to its progeny.
    • A piece of alien DNA has no the sequence called Origin of
    replication (ori) needed for starting replication. So, it
    cannot multiply itself in the progeny cells of the organism.
    • Hence alien DNA is integrated into the recipient genome
    (it has ori). It multiplies & inherits along with host DNA.

    www.bankofbiology.com
    Principles of Biotechnology

    • The process of joining and inserting a foreign


    piece of DNA into a host organism to produce
    new genetic combinations is called
    recombinant DNA technology.
    • First recombinant DNA (rDNA) was produced by Stanley Cohen Herbert Boyer
    Stanley Cohen & Herbert Boyer (1972).
    • They isolated an antibiotic resistance gene
    (piece of DNA) from a plasmid of Salmonella
    typhimurium. It was linked with a plasmid
    vector and transferred into E. coli. As a result,
    the gene was expressed & multiplied in E. coli. Salmonella typhimurium

    www.bankofbiology.com
    www.bankofbiology.com
    TOOLS OF RECOMBINANT DNA TECHNOLOGY

    Restriction
    Enzymes Cloning Competent
    (‘molecular Vectors Host
    scissors’)

    www.bankofbiology.com
    Need Full Version...?
    WhatsApp: 89215 10476

    You might also like

    pFad - Phonifier reborn

    Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

    Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


    Alternative Proxies:

    Alternative Proxy

    pFad Proxy

    pFad v3 Proxy

    pFad v4 Proxy