Janada Karimu—Seminar Slide

Isolation and Identification of Staphylococcus aureus in
patients with wound infection in State Specialist

.Hospital, Maiduguri, Nigeria
PRESENTED BY
JANADA KARIMU
19/08/06/010
SUPERVISED BY
Mr. Hassan Ibrahim Alfaki
A PROJECT DEFENSE PROPORSAL PRESENTED TO THE DEPARTMENT OF

MICROBIOLOGY , FACULTY OF SCIENCE, UNIVERSTY OF MAIDUGURI, BORNO
.STATE
Title slide
Introduction
Statement of the Problem
Justification of the Study
Aim of the study
Objectives of the Study
Materials and methods
Expected Outcome
Conclusion
References
Bacterial contamination of wounds is an important cause of •
mortality. Rapidly emerging nosocomial pathogens and the problem
of multi-drug resistance necessitate periodic review of antibiogram
.pattern of organisms isolated in wounds (Mehta et al., 2007)
Staphylococcus aureus is a facultative anaerobic Gram-positive •

coccal bacterium. It is the most common specie of Staphylococcus
to cause Staphylococcal infection. Staphylococcus aureus has
emerged as one of the mainly important human pathogens, and has
over the past numerous decades, been a leading foundation of
hospital and community-acquired infections (Johnssons et al.,
.2004; Loffler et al., 2005, Shittu and Johnson, 2006)
:Statement of the Problem
Wound infections are a major health

concern, particularly in hospitals, due to the
prevalence of multidrug-resistant
Staphylococcus aureus. In Maiduguri, the
lack of local data on S. aureus prevalence
and resistance patterns complicates
treatment options and increases patient
morbidity (Kumar et al., 2020; Shrestha et
.al., 2021)
:Justification of the Study
This research will provide data on S. aureus in

wound infections, guiding effective treatment
strategies and informing antibiotic stewardship
programs. The findings will be relevant for
improving patient outcomes and addressing the
challenge of antibiotic resistance in healthcare
.settings (WHO, 2021)
:Aim of the study
The aim is to isolate and identify Staphylococcus aureus in
patients with wound infections in State Specialist Hospital,
.Maiduguri, Nigeria
Objectives of the
:Study
To isolate, characterize, and identify Staphylococcus aureus •
.from wound specimens collected from patients at SSHM
To determine the presence of Staphylococcal infection in •
.wound specimens collected from patients at SSHM
To characterize the isolated Staphylococcus aureus strains •
from wound specimens collected from patients at State
.Specialist Hospital, Maiduguri, Nigeria
Materials and
:methods
Study Area: The research will be carried out in
Maiduguri, Borno State, at the State Specialist
.Hospital
Population Study: Patients undergoing treatment

.at the hospital will be the target population
Sample Collection: A total of 60 wound swabs will

be collected under aseptic conditions and
.processed within one hour after collection
Materials and
:methods
Laboratory Procedure
Media Preparation: Mannitol Salt Agar (MSA)

will be prepared for isolating Staphylococcus
.aureus, sterilized, and poured into petri dishes
Inoculation: Samples will be inoculated onto

MSA and incubated at 37°C for 24 hours, with
colonies being sub-cultured for further
.purification
Materials and
:methods
Identification Technique
Gram Staining: Gram staining will be used to •

.identify bacterial morphology
:Biochemical Tests •
Catalase Test: This test will differentiate S. °
aureus (catalase positive) from non-catalase
.producers
Coagulase Test: S. aureus will be identified °
based on a positive coagulase reaction
Materials and
:methods
Antibiotic Sensitivity Testing
Disk Diffusion Method: The antibiotic •

susceptibility of the isolates will be tested using
standard discs of antibiotics such as rifampicin,
.ciprofloxacin, gentamicin, and ceftriaxone
Expected
:Outcome
A high prevalence of S. aureus in wound
.infections is expected (45% of samples)
Resistance to antibiotics such as

cefuroxime, ampiclox, and amoxicillin will
.likely be observed
Antibiotics like rifampicin, levofloxacin, and

.ciprofloxacin are expected to be effective
:Conclusion
The study will investigate Staphylococcus

aureus in wound infections at Maiduguri's State
Specialist Hospital, focusing on its prevalence
and antibiotic resistance. Findings will guide
treatment strategies, enhancing patient care
while contributing to global efforts against
.antimicrobial resistance
Mehta, Y., Kaur, H., & Kumari, M. (2007). "Bacterial contamination of wounds: An important
cause of mortality." Journal of Infection and Public Health, 1(1), 41-47. doi:10.1016/S1876-
.0341(08)60008-0
Johnsson, G., Kallio, R., & Bjerknes, I. (2004). "Staphylococcus aureus: A major human
pathogen." Clinical Microbiology and Infection, 10(7), 670-678. doi:10.1111/j.1469-
.0691.2004.01093.x
Löffler, B., Peters, G., & Becker, K. (2005). "Staphylococcus aureus and its role in hospital and
community-acquired infections." Clinical Microbiology Reviews, 18(4), 616-638.
.doi:10.1128/CMR.18.4.616-638.2005
Shittu, A.O., & Johnson, W.M. (2006). "Antimicrobial susceptibility patterns of Staphylococcus
aureus in Nigeria: A review." African Journal of Microbiology Research, 9(13), 982-992.
.doi:10.5897/AJMR2006.000-1255
Kumar, A., Prakash, S., & Ghosh, A. (2020). "Antimicrobial Resistance in Staphylococcus
aureus: A Review." Journal of Microbiology and Biotechnology, 36(7), 953-961.
doi:10.4014/jmb.2002.02016
Shrestha, L.B., Dhakal, S., & Acharya, S. (2021). "Prevalence of Methicillin-Resistant
Staphylococcus aureus in Wound Infection." International Journal of Infectious Diseases, 106,
330-335. doi:10.1016/j.ijid.2021.04.002
World Health Organization (WHO). (2021). "Global Antimicrobial Resistance and Use

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Isolation and Identification of Staphylococcus aureus in

patients with wound infection in State Specialist

A PROJECT DEFENSE PROPORSAL PRESENTED TO THE DEPARTMENT OF

Staphylococcus aureus is a facultative anaerobic Gram-positive •

Wound infections are a major health

This research will provide data on S. aureus in

The aim is to isolate and identify Staphylococcus aureus in

patients with wound infections in State Specialist Hospital,

Population Study: Patients undergoing treatment

Sample Collection: A total of 60 wound swabs will

Media Preparation: Mannitol Salt Agar (MSA)

Inoculation: Samples will be inoculated onto

Gram Staining: Gram staining will be used to •

Disk Diffusion Method: The antibiotic •

Resistance to antibiotics such as

Antibiotics like rifampicin, levofloxacin, and

The study will investigate Staphylococcus

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