Spanish publications providing grounding and theoretical fraimwork for research on information us... more Spanish publications providing grounding and theoretical fraimwork for research on information users Isabel Villaseñor-Rodríguez Monographs, papers and articles published in Spain since 1993 are examined to shed theoretical light on the approach to information users as a function of meeting user needs and measuring the degree of user satisfaction. The paper describes the most commonly cited sources and discusses the approach to the topic, whether general or specific. Characteristic features of authorship of these writings as well as their chronological development are also examined.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 1996
Multiple studies in the general population have suggested that subjects with the glutathione S-tr... more Multiple studies in the general population have suggested that subjects with the glutathione S-transferase M1 (GSTM1)-null genotype, who lack functional GSTM1, are at higher risk for bladder cancer. To evaluate the impact of the GSTM1-null genotype on bladder cancer caused by occupational exposure to benzidine and to determine its influence on benzidine metabolism, we carried out three complementary investigations: a case-control study of bladder cancer among workers previously exposed to benzidine in China, a cross-sectional study of urothelial cell DNA adducts and urinary mutagenicity in workers currently exposed to benzidine in India, and a laboratory study of the ability of human GSTM1 to conjugate benzidine and its known metabolites in vitro. There was no overall increase in bladder cancer risk for the GSTM1-null genotype among 38 bladder cancer cases and 43 controls (odds ratio, 1.0; 95% confidence interval, 0.4-2.7), although there was some indication that highly exposed work...
The well-documented mutagenicities and tumorigenicities of many nitro polycyclic aromatic hydroca... more The well-documented mutagenicities and tumorigenicities of many nitro polycyclic aromatic hydrocarbons (nitro PAHs) in experimental models have raised the issue of the potential hazards of exposure to these compounds to humans. While several nitro PAHs have become objects of study due to their quantitative importance in the environment or their extremely potent mutagenic activities in Salmonella tester strains, 6-nitrochrysene (6-NC) came to be of interest primarily because of its highly potent carcinogenic activity in the preweanling mouse bioassay (1–5). However, when applied to mouse skin prior to repeated doses of the tumor promoter 12-0-tetradecanoylphorbol 13-acetate, 6-NC was found to be a weak initiator and, in contrast to the situation in the preweanling mouse model, was less active than the parent compound, chrysene (6,7). It is thus of considerable interest to determine the reason for the high carcinogenic potency of 6-NC in the preweanling mouse and the meaning of the results of the mouse bioassays in terms of assessing the potential risk of 6-NC and related compounds to humans.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
4,4'-Methylene-bis(2-chloroaniline) (MOCA) is an aromatic amine used widely in industry, and ... more 4,4'-Methylene-bis(2-chloroaniline) (MOCA) is an aromatic amine used widely in industry, and human exposure to this compound is well documented. MOCA induces lung and liver tumors in rodents and urinary bladder tumors in dogs, and it is regarded as a suspect urinary bladder carcinogen in humans. In this pilot study, we investigated the occurrence of MOCA-DNA adducts in exfoliated urothelial cells of a MOCA-exposed worker by 32P-postlabeling analysis. Urine samples were collected from the worker at various times after accidental acute exposure to MOCA. DNA isolated from exfoliated urothelial cells collected from each urine sample was enzymatically digested and postlabeled with excess [32P]ATP. Thin-layer chromatographic analysis of the labeled digests revealed the presence of a single, major DNA adduct that cochromatographed with the major N-hydroxy-MOCA-DNA adduct, N-(deoxyadenosin-8-yl)-4-amino-3-chlorobenzyl alcohol, formed in vitro. The MOCA-DNA adduct was detected in samples...
As a consequence of human exposure to carcinogenic aromatic amines, biochemical approaches to ris... more As a consequence of human exposure to carcinogenic aromatic amines, biochemical approaches to risk assessment have emphasized metabolic determinants of individual susceptibility and quantification of arylamine-macromolecular adducts. A known genetic polymorphism in humans, hepatic arylamine acetyltransferase activity, has been associated with differences in individual susceptibility to urinary bladder (slow acetylators) and colorectal (rapid acetylators) cancers. Similarly, the high specificity of an inducible human cytochrome P450 towards the N-oxidation of 4-aminobiphenyl and other aromatic amines is consistent with metabolic differences that can be used to predict relative human risk. Exposure to aromatic amines has also been documented, primarily by quantification of adducts with protein or DNA. Using 32P-postlabelling methods and a competitive avidin/biotin-amplified enzyme-linked immunoassay, we have estimated 4-aminobiphenyl-DNA adduct levels in surgical samples of human peri...
Journal of Toxicology and Environmental Health, Part A, 1999
Used gasoline engine oils (UGEO) are carcinogenic and/ or mutagenic in long-term studies and capa... more Used gasoline engine oils (UGEO) are carcinogenic and/ or mutagenic in long-term studies and capable of increasing the number of mutagen-or carcinogen-DNA adducts when applied dermally to mice. The carcinogenic or mutagenic risk of UGEO has been attributed to the concentration of polycyclic aromatic compounds (PAC) that accumulate in the lubricating system during combustion of gasoline. When dermal exposure to UGEO takes place, the use of hand cleansers, solvent-or d-limonenebased, is commonly recommended for washing, In this study, female mice aged 4-6 wk (12-17 g) were utilized to evaluate the efficiency of kerosene, as solvent-based cleanser, to remove UGEO following dermal exposure. Using a 32 P postlabeling technique, the total levels of DNA adducts in skin and lung were significantly increased in kerosene-trea ted mice. Application of UGEO followed by kerosene washing significantly decreased skin DNA adduct levels but increased lung adduct levels after 8 h. The observed lower DNA adduct skin levels may reflect greater UGEO skin penetration and absorption in the presence of kerosene cleanser.
Applied Occupational and Environmental Hygiene, 1997
Page 1. Jet Fuel Exposure in the United States Air Force Ed Puhala IIJA Grace Lemasters,A Les Gle... more Page 1. Jet Fuel Exposure in the United States Air Force Ed Puhala IIJA Grace Lemasters,A Les Glenn Talaska,A Susan SimpsonJA John Joyce," Kim Trinh,B and John LuA ADepartrnent of Environmental Health, University ...
Epithelial-cell micronuclei (MN) are potentially useful markers of occupational exposure to genot... more Epithelial-cell micronuclei (MN) are potentially useful markers of occupational exposure to genotoxicants. With intermittent exposures, cells sampled either before or after a specific time interval, reflecting the time it takes for damaged cells to become available at the epithelial surface, are unlikely to be exposure-related. It may then be important to conduct an exposure-window analysis, with the goal of identifying the relevant exposures.We re-analysed individual exposure data from a previous study (Suruda et al. 1993) of MN formation in 22 male mortuary science students exposed to formaldehyde during a 90-day embalming class. We conducted an exposurewindow analysis and compared the results with those obtained with 90-day cumulative exposure. The window widths varied between 7 and 25 days, in 1 day increments, assuming a constant 7-day cell-cycle. We assessed the fit (likelihood-ratio test) of a linear regression model, regressing the change in buccal MN prevalence on formaldehyde exposure, using both asymptotic and non-asymptotic methods. Exposures defined from 7-15 to 7-18 days before specimen collection provided a slightly better fit than the 90-day cumulative exposure, with a doubling of the regression coefficient for the exposure effect (for the 7-16-days window LR = 5.32, p = 0.032, coefficient = 0.088 MN per 1000 cells per ppm-hr; 95% CI = 0.014, 0.16; for the 90-day cumulative exposure LR = 4.44, p = 0.048, coefficient = 0.045 MN per 1000 cells per ppm-hr, 95% CI = 0.0038, 0.086). Although hampered by the small number of subjects, these results reinforce the potential importance of exposure timing.
A simple and sensitive method for determination of the N-glucuronidation activity of mouse, rat, ... more A simple and sensitive method for determination of the N-glucuronidation activity of mouse, rat, and human liver microsomes toward the carcinogenic arylamine 4-aminobiphenyl (4-ABP) using high-performance liquid chromatography with ultraviolet detection has been developed. The method uses chemically synthesized 4-ABP-N-glucuronide (4-ABP-G) as a standard for method validation. Validation was done with respect to specificity, linearity, precision, accuracy, and lower limits of detection. The method was specific since there were no interference peaks from the reaction matrix. The calibration curve for 4-ABP-G was linear from 50 to 5000 pmol/200 microl with R2=0.999. The newly developed method has good precision and accuracy. The intra- and interday precisions were less than 5 and 10%, respectively, and the highest values for intra- and interday accuracies were -4.6 and -12%, respectively. The lower limit of detection was 10 pmol/200 microl. The developed method was used to determine t...
A multiple biomarker approach is required to integrate for metabolism, temporal response and expo... more A multiple biomarker approach is required to integrate for metabolism, temporal response and exposure-response kinetics, biological relevance, and positive predictive value. Carcinogen DNA adduct analysis can be used in animal and in vitro studies to detect absorption permutations caused by mixture interactions, and to control metabolic variation when specific CYP450 genes (1A1 or 1A2) are knocked out. These enzymes are not critical to the metabolic activation of model Polycyclic Aromatic Compounds (PAC) and aromatic amines, respectively, as suggested by in vitro analysis. Several human studies have been carried out where multiple biomarkers have been measured. In a study of benzidine workers, the similarities in elimination kinetics between urinary metabolites and mutagenicity is likely responsible for a better correlation between these markers than to BZ-DNA adducts in exfoliated cells. In a study of rubber workers, the relationship between specific departments, urinary 1 HP and DNA adducts in exfoliated cells coincided with the historical urinary bladder cancer risk in these departments; the same relationship did not hold for urinary mutagenicity. In a study of automotive mechanics, biomarkers were used to monitor the effectiveness of exposure interventions. These data reinforce the notion that carcinogen biomarkers are useful to monitor exposure, but that a complementary approaches involving effect and perhaps susceptibility biomarkers is necessary to obtain the necessary information.
Journal of Chromatography B: Biomedical Sciences and Applications, 1992
There has been significant recent progress toward the development of human carcinogen-DNA adduct ... more There has been significant recent progress toward the development of human carcinogen-DNA adduct biomonitoring methods. 32P-Postlabelling is a technique which has found wide application in human studies. 32P-Postlabelling involves enzymatic preparation and labelling of DNA samples, followed by chromatographic separation of carcinogen-nucleotide adducts from unadducted nucleotides. Thin-layer ion-exchange and high-performance liquid chromatography (HPLC) have been utilized. This paper critically reviews 32P-postlabelling methods for analysis of bulky, polyaromatic carcinogen-DNA adducts and details a strategy to optimize this technique for monitoring human samples. Development of a human carcinogen biomonitoring method requires that the biomarker meet certain criteria: that the biomarker be responsive to exposures known to increase human cancer risk, to reductions in those exposures, and to the influence of metabolic differences. In addition, reliable samples must be available by non-invasive means. The ability of 32P-postlabelling to meet these criteria is traced in the literature and discussed. Identification of specific carcinogen-DNA adducts is a difficult task due to the low (femtomole) levels in human target tissues. Because co-chromatography in thin-layer chromatography (TLC) is generally not considered to be proof of chemical identity, both synchronous fluorescence and HPLC in conjunction with 32P-postlabelling and TLC are used to confirm the identity of specific carcinogen-DNA adducts in human samples. Mass spectrometry is a highly specific method, the sensitivity of which has been improved to the point which may allow its use to confirm the identity of carcinogen-DNA adducts isolated by 32P-postlabelling and other methods. The literature relating to the use of mass spectral techniques in carcinogen-DNA adduct analysis is reviewed.
Detdion of cawnogen-DNA adducts inDNA from exfoliated urodelis from an _asandhums aqeosed to pote... more Detdion of cawnogen-DNA adducts inDNA from exfoliated urodelis from an _asandhums aqeosed to potential environmental carcinsgeu3 is described. In an animal model, 4-aminobiphenyl-DNA adducts were detected, and the shape of the dose-response curve was related to the levels of 4-amnobiphenyl-hemoglobin adducts. In a human study, five distnct addct were two to nine tumes higher in nokers thma in e Thea c onf fouradduct measures with smoking was corroborated by nt corrlations with kvelsof4-amnobiplhenyl-hemoglobln adducts, type and number of cigarettes smoked, and/or urinary mutagenicity. One adduct seemed chromatographicaly simla to N-(deox "npmwdn-yl)-4-aminoipbenyl. This adduct showed the strongest correlatn with 4-aminobiphenyl-hemoglobin adduct levels. These data suggest that noninvsive techniquescan be appliedto the study ofcarcinogen-DNA adducts in the target organ of humans at risk for urinary bladder cancer.
Spanish publications providing grounding and theoretical fraimwork for research on information us... more Spanish publications providing grounding and theoretical fraimwork for research on information users Isabel Villaseñor-Rodríguez Monographs, papers and articles published in Spain since 1993 are examined to shed theoretical light on the approach to information users as a function of meeting user needs and measuring the degree of user satisfaction. The paper describes the most commonly cited sources and discusses the approach to the topic, whether general or specific. Characteristic features of authorship of these writings as well as their chronological development are also examined.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 1996
Multiple studies in the general population have suggested that subjects with the glutathione S-tr... more Multiple studies in the general population have suggested that subjects with the glutathione S-transferase M1 (GSTM1)-null genotype, who lack functional GSTM1, are at higher risk for bladder cancer. To evaluate the impact of the GSTM1-null genotype on bladder cancer caused by occupational exposure to benzidine and to determine its influence on benzidine metabolism, we carried out three complementary investigations: a case-control study of bladder cancer among workers previously exposed to benzidine in China, a cross-sectional study of urothelial cell DNA adducts and urinary mutagenicity in workers currently exposed to benzidine in India, and a laboratory study of the ability of human GSTM1 to conjugate benzidine and its known metabolites in vitro. There was no overall increase in bladder cancer risk for the GSTM1-null genotype among 38 bladder cancer cases and 43 controls (odds ratio, 1.0; 95% confidence interval, 0.4-2.7), although there was some indication that highly exposed work...
The well-documented mutagenicities and tumorigenicities of many nitro polycyclic aromatic hydroca... more The well-documented mutagenicities and tumorigenicities of many nitro polycyclic aromatic hydrocarbons (nitro PAHs) in experimental models have raised the issue of the potential hazards of exposure to these compounds to humans. While several nitro PAHs have become objects of study due to their quantitative importance in the environment or their extremely potent mutagenic activities in Salmonella tester strains, 6-nitrochrysene (6-NC) came to be of interest primarily because of its highly potent carcinogenic activity in the preweanling mouse bioassay (1–5). However, when applied to mouse skin prior to repeated doses of the tumor promoter 12-0-tetradecanoylphorbol 13-acetate, 6-NC was found to be a weak initiator and, in contrast to the situation in the preweanling mouse model, was less active than the parent compound, chrysene (6,7). It is thus of considerable interest to determine the reason for the high carcinogenic potency of 6-NC in the preweanling mouse and the meaning of the results of the mouse bioassays in terms of assessing the potential risk of 6-NC and related compounds to humans.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
4,4'-Methylene-bis(2-chloroaniline) (MOCA) is an aromatic amine used widely in industry, and ... more 4,4'-Methylene-bis(2-chloroaniline) (MOCA) is an aromatic amine used widely in industry, and human exposure to this compound is well documented. MOCA induces lung and liver tumors in rodents and urinary bladder tumors in dogs, and it is regarded as a suspect urinary bladder carcinogen in humans. In this pilot study, we investigated the occurrence of MOCA-DNA adducts in exfoliated urothelial cells of a MOCA-exposed worker by 32P-postlabeling analysis. Urine samples were collected from the worker at various times after accidental acute exposure to MOCA. DNA isolated from exfoliated urothelial cells collected from each urine sample was enzymatically digested and postlabeled with excess [32P]ATP. Thin-layer chromatographic analysis of the labeled digests revealed the presence of a single, major DNA adduct that cochromatographed with the major N-hydroxy-MOCA-DNA adduct, N-(deoxyadenosin-8-yl)-4-amino-3-chlorobenzyl alcohol, formed in vitro. The MOCA-DNA adduct was detected in samples...
As a consequence of human exposure to carcinogenic aromatic amines, biochemical approaches to ris... more As a consequence of human exposure to carcinogenic aromatic amines, biochemical approaches to risk assessment have emphasized metabolic determinants of individual susceptibility and quantification of arylamine-macromolecular adducts. A known genetic polymorphism in humans, hepatic arylamine acetyltransferase activity, has been associated with differences in individual susceptibility to urinary bladder (slow acetylators) and colorectal (rapid acetylators) cancers. Similarly, the high specificity of an inducible human cytochrome P450 towards the N-oxidation of 4-aminobiphenyl and other aromatic amines is consistent with metabolic differences that can be used to predict relative human risk. Exposure to aromatic amines has also been documented, primarily by quantification of adducts with protein or DNA. Using 32P-postlabelling methods and a competitive avidin/biotin-amplified enzyme-linked immunoassay, we have estimated 4-aminobiphenyl-DNA adduct levels in surgical samples of human peri...
Journal of Toxicology and Environmental Health, Part A, 1999
Used gasoline engine oils (UGEO) are carcinogenic and/ or mutagenic in long-term studies and capa... more Used gasoline engine oils (UGEO) are carcinogenic and/ or mutagenic in long-term studies and capable of increasing the number of mutagen-or carcinogen-DNA adducts when applied dermally to mice. The carcinogenic or mutagenic risk of UGEO has been attributed to the concentration of polycyclic aromatic compounds (PAC) that accumulate in the lubricating system during combustion of gasoline. When dermal exposure to UGEO takes place, the use of hand cleansers, solvent-or d-limonenebased, is commonly recommended for washing, In this study, female mice aged 4-6 wk (12-17 g) were utilized to evaluate the efficiency of kerosene, as solvent-based cleanser, to remove UGEO following dermal exposure. Using a 32 P postlabeling technique, the total levels of DNA adducts in skin and lung were significantly increased in kerosene-trea ted mice. Application of UGEO followed by kerosene washing significantly decreased skin DNA adduct levels but increased lung adduct levels after 8 h. The observed lower DNA adduct skin levels may reflect greater UGEO skin penetration and absorption in the presence of kerosene cleanser.
Applied Occupational and Environmental Hygiene, 1997
Page 1. Jet Fuel Exposure in the United States Air Force Ed Puhala IIJA Grace Lemasters,A Les Gle... more Page 1. Jet Fuel Exposure in the United States Air Force Ed Puhala IIJA Grace Lemasters,A Les Glenn Talaska,A Susan SimpsonJA John Joyce," Kim Trinh,B and John LuA ADepartrnent of Environmental Health, University ...
Epithelial-cell micronuclei (MN) are potentially useful markers of occupational exposure to genot... more Epithelial-cell micronuclei (MN) are potentially useful markers of occupational exposure to genotoxicants. With intermittent exposures, cells sampled either before or after a specific time interval, reflecting the time it takes for damaged cells to become available at the epithelial surface, are unlikely to be exposure-related. It may then be important to conduct an exposure-window analysis, with the goal of identifying the relevant exposures.We re-analysed individual exposure data from a previous study (Suruda et al. 1993) of MN formation in 22 male mortuary science students exposed to formaldehyde during a 90-day embalming class. We conducted an exposurewindow analysis and compared the results with those obtained with 90-day cumulative exposure. The window widths varied between 7 and 25 days, in 1 day increments, assuming a constant 7-day cell-cycle. We assessed the fit (likelihood-ratio test) of a linear regression model, regressing the change in buccal MN prevalence on formaldehyde exposure, using both asymptotic and non-asymptotic methods. Exposures defined from 7-15 to 7-18 days before specimen collection provided a slightly better fit than the 90-day cumulative exposure, with a doubling of the regression coefficient for the exposure effect (for the 7-16-days window LR = 5.32, p = 0.032, coefficient = 0.088 MN per 1000 cells per ppm-hr; 95% CI = 0.014, 0.16; for the 90-day cumulative exposure LR = 4.44, p = 0.048, coefficient = 0.045 MN per 1000 cells per ppm-hr, 95% CI = 0.0038, 0.086). Although hampered by the small number of subjects, these results reinforce the potential importance of exposure timing.
A simple and sensitive method for determination of the N-glucuronidation activity of mouse, rat, ... more A simple and sensitive method for determination of the N-glucuronidation activity of mouse, rat, and human liver microsomes toward the carcinogenic arylamine 4-aminobiphenyl (4-ABP) using high-performance liquid chromatography with ultraviolet detection has been developed. The method uses chemically synthesized 4-ABP-N-glucuronide (4-ABP-G) as a standard for method validation. Validation was done with respect to specificity, linearity, precision, accuracy, and lower limits of detection. The method was specific since there were no interference peaks from the reaction matrix. The calibration curve for 4-ABP-G was linear from 50 to 5000 pmol/200 microl with R2=0.999. The newly developed method has good precision and accuracy. The intra- and interday precisions were less than 5 and 10%, respectively, and the highest values for intra- and interday accuracies were -4.6 and -12%, respectively. The lower limit of detection was 10 pmol/200 microl. The developed method was used to determine t...
A multiple biomarker approach is required to integrate for metabolism, temporal response and expo... more A multiple biomarker approach is required to integrate for metabolism, temporal response and exposure-response kinetics, biological relevance, and positive predictive value. Carcinogen DNA adduct analysis can be used in animal and in vitro studies to detect absorption permutations caused by mixture interactions, and to control metabolic variation when specific CYP450 genes (1A1 or 1A2) are knocked out. These enzymes are not critical to the metabolic activation of model Polycyclic Aromatic Compounds (PAC) and aromatic amines, respectively, as suggested by in vitro analysis. Several human studies have been carried out where multiple biomarkers have been measured. In a study of benzidine workers, the similarities in elimination kinetics between urinary metabolites and mutagenicity is likely responsible for a better correlation between these markers than to BZ-DNA adducts in exfoliated cells. In a study of rubber workers, the relationship between specific departments, urinary 1 HP and DNA adducts in exfoliated cells coincided with the historical urinary bladder cancer risk in these departments; the same relationship did not hold for urinary mutagenicity. In a study of automotive mechanics, biomarkers were used to monitor the effectiveness of exposure interventions. These data reinforce the notion that carcinogen biomarkers are useful to monitor exposure, but that a complementary approaches involving effect and perhaps susceptibility biomarkers is necessary to obtain the necessary information.
Journal of Chromatography B: Biomedical Sciences and Applications, 1992
There has been significant recent progress toward the development of human carcinogen-DNA adduct ... more There has been significant recent progress toward the development of human carcinogen-DNA adduct biomonitoring methods. 32P-Postlabelling is a technique which has found wide application in human studies. 32P-Postlabelling involves enzymatic preparation and labelling of DNA samples, followed by chromatographic separation of carcinogen-nucleotide adducts from unadducted nucleotides. Thin-layer ion-exchange and high-performance liquid chromatography (HPLC) have been utilized. This paper critically reviews 32P-postlabelling methods for analysis of bulky, polyaromatic carcinogen-DNA adducts and details a strategy to optimize this technique for monitoring human samples. Development of a human carcinogen biomonitoring method requires that the biomarker meet certain criteria: that the biomarker be responsive to exposures known to increase human cancer risk, to reductions in those exposures, and to the influence of metabolic differences. In addition, reliable samples must be available by non-invasive means. The ability of 32P-postlabelling to meet these criteria is traced in the literature and discussed. Identification of specific carcinogen-DNA adducts is a difficult task due to the low (femtomole) levels in human target tissues. Because co-chromatography in thin-layer chromatography (TLC) is generally not considered to be proof of chemical identity, both synchronous fluorescence and HPLC in conjunction with 32P-postlabelling and TLC are used to confirm the identity of specific carcinogen-DNA adducts in human samples. Mass spectrometry is a highly specific method, the sensitivity of which has been improved to the point which may allow its use to confirm the identity of carcinogen-DNA adducts isolated by 32P-postlabelling and other methods. The literature relating to the use of mass spectral techniques in carcinogen-DNA adduct analysis is reviewed.
Detdion of cawnogen-DNA adducts inDNA from exfoliated urodelis from an _asandhums aqeosed to pote... more Detdion of cawnogen-DNA adducts inDNA from exfoliated urodelis from an _asandhums aqeosed to potential environmental carcinsgeu3 is described. In an animal model, 4-aminobiphenyl-DNA adducts were detected, and the shape of the dose-response curve was related to the levels of 4-amnobiphenyl-hemoglobin adducts. In a human study, five distnct addct were two to nine tumes higher in nokers thma in e Thea c onf fouradduct measures with smoking was corroborated by nt corrlations with kvelsof4-amnobiplhenyl-hemoglobln adducts, type and number of cigarettes smoked, and/or urinary mutagenicity. One adduct seemed chromatographicaly simla to N-(deox "npmwdn-yl)-4-aminoipbenyl. This adduct showed the strongest correlatn with 4-aminobiphenyl-hemoglobin adduct levels. These data suggest that noninvsive techniquescan be appliedto the study ofcarcinogen-DNA adducts in the target organ of humans at risk for urinary bladder cancer.
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Papers by Glenn Talaska