Optimisation of Bioluminescent Reporters for Use with Mycobacteria
Figure 6
Bioluminescence correlates with substrate concentration at low concentrations.
Luminescence (given as relative light units [RLUs]) of M. smegmatis pMVhsp+FFluc (a) and M. smegmatis pMVhsp+Gluc (b) was measured with integration times of 5 s and 0.1 s respectively. The substrate concentrations assayed ranged from 20 to 4710 µM luciferin for FFluc, and from 0.05 to 400 µM coelenterazine for Gluc. Means and standard deviations (smaller than symbols) of six replicates are shown.