Altered Hippocampal Lipid Profile Following Acute Postnatal Exposure to Di(2-Ethylhexyl) Phthalate in Rats
Abstract
:1. Introduction
2. Method
2.1. Materials
2.1.1. Animals
2.1.2. DEHP Injections
2.1.3. Tissue Processing and Lipid Extraction
2.1.4. High Performance Liquid Chromatography and Mass Spectrometry
2.2. Data Analyses
2.3. Statistical Analyses
3. Results
m/z | Total Carbons: Double Bonds | Predicted Lipid Species *** | Fold Change | Relative Concentration of Lipid Per gram of Hippocampus | ||
---|---|---|---|---|---|---|
Males | Females | Males | Females | |||
468.3 | (14:0) | LPC (14:0) | 2.03 ↑ DEHP b | --- a | V: 0.88±0.16 D: 1.79±0.19 | V: 2.83±0.31 D: 1.53±0.80 |
732.6 | (32:1) | PC (16:1/16:0) | --- | 3.29 ↑ DEHP b | V: 1766.76±366.28 D: 4056.97±1631.86 | V: 1646.13±609.25 D: 5412.37±1249.25 |
758.6 | (34:2) | PC (18:1/16:1) | --- | 3.08 ↑ DEHP b | V: 321.78±96.55 D: 683.92±333.23 | V: 294.26±43.02 D: 906.64±203.36 |
760.6 | (34:1) | PC (18:1/16:0) | --- | 2.68 ↑ DEHP c | V: 6260.94±2437.35 D: 11,537.41±5966.57 | V: 5880.58±1271.79 D: 15,783.46±3544.24 |
764.6 | (35:6) | PC (18:4/17:2) | --- | 4.74 ↑ DEHP c | V: 37.56±21.54 D: 135.41±110.99 | V: 31.33±6.21 D: 148.64±44.32 |
782.6 | (36:4) | PC (18:2/18:2) | --- | 2.87 ↑ DEHP b | V: 1109.55±262.17 D: 2906.58±1580.80 | V: 1241.84±302.37 D: 3568.77±666.00 |
788.7 | (36:1) | PC (18:1/18:0) | --- | 2.72 ↑ DEHP c | V: 2719.13±1211.77 D: 4175.92±2152.94 | V: 2211.56±588.64 D: 6023.49±1296.15 |
806.6 | (38:6) | PC (20:3/18:3) | --- | 2.82 ↑ DEHP b | V: 536.77±210.81 D: 1355.15±723.62 | V: 518.52±84.55 D: 1463.46±210.33 |
808.6 | (38:5) | PC (20:3/18:2) | --- | 3.00 ↑ DEHP b | V: 291.38±121.59 D: 679.25±365.05 | V: 273.06±48.09 D: 819.87±157.10 |
834.6 | (40:6) | PC (20:3/20:3) | --- | 2.91 ↑ DEHP c | V: 206.52±90.83 D: 336.29±209.91 | V: 158.47±44.99 D: 461.34±103.11 |
838.6 | (40:4) | PC (20:3/20:1) | --- | 3.40 ↑ DEHP b | V: 38.42±16.02 D: 97.66±45.29 | V: 64.59±20.90 D: 219.66±50.26 |
Total LPC | --- | --- | V: 3223.17±1489.33 D: 2948.37±698.58 | V: 2635.85±523.92 D: 3920.04±427.90 | ||
Total PC | --- | 2.63 ↑ DEHP c | V: 37,287.72±6179.05 D: 51,856.77±27,679.69 | V: 23,354.76±4364.30 D: 6144.71±18,613.39 | ||
Total Lipids (LPC, PC and SM) | --- | 2.69 ↑ DEHP b | V: 52,528.43±7321.69 D: 73,137.90±39,015.46 | V: 32,341.13±5807.19 D: 86,903.93±26,297.89 |
m/z | Long-chain Base | N-Acyl | Predicted Lipid Species *** | Fold Change | Relative Concentration of Lipid Per gram of Hippocampus | ||
---|---|---|---|---|---|---|---|
Males | Females | Males | Females | ||||
731.6 | Sphingosine | (18:0) | SM (d18:1/18:0) | --- a | 3.22 ↑ DEHP b | V: 2296.7±644.2 D:4757.1±2416.1 | V: 1914.61±623.75 D: 6159.77±1362.17 |
733.6 | Sphinganine | (18:0) | SM (d18:0/18:0) | --- | 3.08 ↑ DEHP b | V: 1665.38±527.75 D: 4001.54±1816.04 | V: 1411.97±141.86 D: 4354.38±916.31 |
761.7 | Sphinganine | (20:0) | SM (d18:0/20:0) | --- | 2.69 ↑ DEHP c | V: 2953.28±1107.66 D: 5491.13±2792.30 | V: 2717.63±598.07 D: 7310.65±1663.55 |
787.7 | Sphingosine | (22:0) | SM (d18:1/22:0) | --- | 2.76 ↑ DEHP b | V: 702.33±308.72 D: 1185.33±604.79 | V: 522.44±88.71 D: 1442.20±309.83 |
789.7 | Sphinganine | (22:0) | SM (d18:0/22:0) | --- | 2.61 ↑ DEHP c | V: 1237.11±551.36 D: 1824.85±937.12 | V: 1032.04±291.51 D: 2696.53±573.86 |
807.6 | Sphingosine | (24:4) | SM (d18:1/24:4) | --- | 3.47 ↑ DEHP b | V: 264.06±99.29 D: 644.89±339.61 | V: 219.04±44.15 D: 760.28±120.84 |
Total SM | --- | 2.83 ↑ DEHP b | V: 15,240.71±1142.64 D: 21,281.12±11,339.16 | V: 8986.37±1599.90 D: 25,454.22±7687.39 |
4. Discussion
5. Conclusion
Acknowledgements
Author Contributions
Abbreviations
Conflicts of Interest
References
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Smith, C.A.; Farmer, K.; Lee, H.; Holahan, M.R.; Smith, J.C. Altered Hippocampal Lipid Profile Following Acute Postnatal Exposure to Di(2-Ethylhexyl) Phthalate in Rats. Int. J. Environ. Res. Public Health 2015, 12, 13542-13559. https://doi.org/10.3390/ijerph121013542
Smith CA, Farmer K, Lee H, Holahan MR, Smith JC. Altered Hippocampal Lipid Profile Following Acute Postnatal Exposure to Di(2-Ethylhexyl) Phthalate in Rats. International Journal of Environmental Research and Public Health. 2015; 12(10):13542-13559. https://doi.org/10.3390/ijerph121013542
Chicago/Turabian StyleSmith, Catherine A., Kyle Farmer, Hyunmin Lee, Matthew R. Holahan, and Jeffrey C. Smith. 2015. "Altered Hippocampal Lipid Profile Following Acute Postnatal Exposure to Di(2-Ethylhexyl) Phthalate in Rats" International Journal of Environmental Research and Public Health 12, no. 10: 13542-13559. https://doi.org/10.3390/ijerph121013542
APA StyleSmith, C. A., Farmer, K., Lee, H., Holahan, M. R., & Smith, J. C. (2015). Altered Hippocampal Lipid Profile Following Acute Postnatal Exposure to Di(2-Ethylhexyl) Phthalate in Rats. International Journal of Environmental Research and Public Health, 12(10), 13542-13559. https://doi.org/10.3390/ijerph121013542