The University of Kentucky Body Composition Core Laboratory (BCCL) located in the Seaton Center building (Director: Jody L. Clasey, Ph.D., FACSM; Medical Director: Ralph Miller, MD, ScD) is a human based laboratory facility specifically designed to provide state of the art body composition analysis for research and clinical investigations. The BCCL is an estimated 1020 square feet laboratory space (including a separate 180 square feet Bone Densitometry room, a separate 180 square feet Air Displacement Plethsymography and Anthropometric room, and 360 square feet sample storage and processing analyses rooms) housed in the Department of Kinesiology and Health Promotion. The BCCL contains the necessary equipment to perform total body density (hydrostatic weighing and air displacement plethysmography), total body and intra/extra-cellular water, single and multiple frequency bioelectric impedance analyses, and total body and regional bone mineral content and density measurements, thus allowing determination of in vivo body composition analysis using multi-compartment modeling techniques.
Other: A workshop (approximately 125 square feet) containing tools and equipment for basic repair and design of necessary equipment is located adjacent to the Body Composition Core Laboratory.
Equipment:
Total Body and Regional Bone Densitometer:
Lunar DPX-IQ: Net F Network Densitometer; Full size DXA scan table; Acquisition/Analysis of AP spine density, proximal femur density, total body bone density, body composition analysis and report generator software; Pentium II Acquisition computer MMX with 233 MHz, 3.2 GB Hard Drive, 32 MB RAM, 4MB Video RAM, CD ROM, Fax Modem, 17” Color Monitor, HP Color Printer, Zip drive and remote support software; Pentium II workstation computer MMX with 233 MHz, 3.2 GB Hard Drive, 32 MB RAM, 4MB Video RAM, CD ROM, Fax Modem, 17” Color Monitor, HP Color Printer, Zip drive and remote support software; Network hardware and multitasking software DICOM; DICOM export, print and storage. Software purchased: Total body, AP lumbar spine, proximal femur, forearm/hand, orthopedic, lateral spine, small animal, pediatric, and dual femur. Two Laptop computers with DPX-IQ software used for auxiliary analysis purpose. Additional encapsulated phantom kit and leg positioner for determining quality control among collaborative institutions.
Total Body and Extracellular Water Measurement Equipment:
Single-beam infrared spectrometer with a 4-mm fixed filter with a temperature-controlled flow-through sample cell (Miran 1FF, Foxboro, Foxboro, MA); Automated Deuterium Oxide Miran (ADOM) custom made automation system for sample analysis (On-site Instruments, Lewis Center, OH); True-rms 87/E III Multimeter with 0.0001 mV digital display (Fluke Corp., Everett, WA); DEWAR flasks and tubing apparatus for respiratory water sample collection (PGC Scientifics Corp., Frederick, MD); Spectronic Genesys 5 (Thermo Spectronic, Rochester, NY)
Laboratory Sample Preparation and Analysis Equipment:
Electronic Balance (Scientech, Inc., Boulder, CO); Clinical Incubator (Lab-Line Instruments, Inc., Melrose Park, IL); -200 Sample Storage Freezer (Sears, Roebuck and Co., Hoffman Estates, IL); Biological Safety Cabinet (NuAire, Inc., Plymouth, MN); Water Purification System (Millipore Corp., Bedford, MA); Refrigerated Superspeed Centrifuge (Sorvall; Newtown, CT)
Hydrostatic Weighing (Total Body Density) Equipment:
Hydrostatic weighing tank; 30-lb Autopsy Scale (Toledo, Ohio); 500 lb. Load Cell Force Transducer (Interface Inc., Scottsdale, AZ); Residual lung volume measurement system (Exertech Body density Measurement Systems, Dresbach, MN); Chart recorder (OmniScribe series D5000 recorder); Digital Weighing scale Model DI-10 (American Scale Corp., Louisville, KY)
Air Displacement Plethysmography Equipment:
BOD POD Body Composition System including: 1 BOD POD Chamber, Computer Operating System with 166 MHz Pentium Processor, 16 MB RAM, 1.2 GB Hard Drive, 1.44 MB Floppy Drive, SVGA Monitor; 1 Digital Scale with Computer Interface, 1 set of Calibration Standards, 1 Software License, 1 Inkjet Printer, Tubing and Filter Kits (Life Measurement Instruments, Concord, CA).
Single Frequency Bioelectrical Impedance Analysis (BIA):
Biodynamics Model 310 Body Composition Analyzer (Seattle, WA); Omron Body Logic Pro Body Fat Analyzer, Model HBF-300 (Vernon Hills, IL), Tanita TBF 515 Body Fat Monitor/Scale (Arlington Heights, IL); 5 BodyStat 1500 (Isle of Man, United Kingdom).
Multi-Frequency Bioelectical Impedance Analysis (BIA):
Bodystat Multiscan 5000 Body Composition Analyzer (Isle of Man, United Kingdom); Bodystat Quadscan Body Composition Analyzer (Isle of Man, United Kingdom).
Anthropometric Equipment:
Harpenden Skinfold Caliper (British Indicators Ltd. West Sussex, United Kingdom); Lange Skinfold Calipers (Cambridge Scientific Industries, Inc., Cambridge, MD); Rosscraft steel anthropometric tape (Creative Health Products, Plymouth, MI), Holtain Sliding Anthropometer (United Kingdom); Tommy 2 Sliding Caliper (Creative Health Products, Plymouth, MI).
Glucose Lactate Analyzer:
YSI 2300 STAT Plus Glucose and Lactate Analyzer (YSI Incorporated, Yellow Springs, OH); Analyzes whole blood or plasma glucose and lactate concentrations, rapid blood analysis; 24 position turntable.