Chlorpyrifos is a crystalline organophosphate insec ticide. A simple and rapid reverse phase high... more Chlorpyrifos is a crystalline organophosphate insec ticide. A simple and rapid reverse phase high-perfo rmance liquid chromatographic method using UV detection wa s developed and validated for the analysis of chlor pyrifos in selected vegetable samples like cabbage, cauliflowe r and capsicum under isocratic conditions. A Kromos il C18 analytical column (150 mm ◊ 4.6 mm I.D., 5 µm particle size) and a mobile phase constituting ac etonitrile: 1 mM phosphate buffer (85:15 v/v, pH 4.5 adjusted with o rthophosphoric acid) were used. The flow rate was 1 ml/min and the analyses performed using ultraviolet detector a t a wavelength of 230 nm. The method response was l inear over the concentration range of 2 to 12 µg/ml. The developed method was validated as per sta ndard guidelines. Validation of the developed method demonstrated sys tem suitability, sensitivity, accuracy, precision and selectivity of proposed method. This rapid and simple method wa s successfully applied in the estimation ...
The objective of the present study was to indentify the activity of Glutamic Oxalacetic transmina... more The objective of the present study was to indentify the activity of Glutamic Oxalacetic transminase (GOT) And Glutamic Pyruvic transaminase (GPT) in various tissue homogenates of the freshwater fish Catla catla. It revealed that the extent of Glutamic Pyruvic transaminase activity to be higher than that of Glutamic Oxalacetic transminase activity. A general decline in transaminases activity as a function of sublethal concentration of heavy metal chromium was evidenced which reflects the injury to lysosome due to stressing the biological system. Dose as well as exposure period dependent statistically significant (P<0.05) inhibition in Glutamic Oxalacetic transminase activity in liver and brain and Glutamic Pyruvic transaminase activity in liver and kidney of Catla catla are advocated as bioindicators of water borne heavy metal chromium stress.
Chlorpyrifos is a crystalline organophosphate insec ticide. A simple and rapid reverse phase high... more Chlorpyrifos is a crystalline organophosphate insec ticide. A simple and rapid reverse phase high-perfo rmance liquid chromatographic method using UV detection wa s developed and validated for the analysis of chlor pyrifos in selected vegetable samples like cabbage, cauliflowe r and capsicum under isocratic conditions. A Kromos il C18 analytical column (150 mm ◊ 4.6 mm I.D., 5 µm particle size) and a mobile phase constituting ac etonitrile: 1 mM phosphate buffer (85:15 v/v, pH 4.5 adjusted with o rthophosphoric acid) were used. The flow rate was 1 ml/min and the analyses performed using ultraviolet detector a t a wavelength of 230 nm. The method response was l inear over the concentration range of 2 to 12 µg/ml. The developed method was validated as per sta ndard guidelines. Validation of the developed method demonstrated sys tem suitability, sensitivity, accuracy, precision and selectivity of proposed method. This rapid and simple method wa s successfully applied in the estimation ...
The objective of the present study was to indentify the activity of Glutamic Oxalacetic transmina... more The objective of the present study was to indentify the activity of Glutamic Oxalacetic transminase (GOT) And Glutamic Pyruvic transaminase (GPT) in various tissue homogenates of the freshwater fish Catla catla. It revealed that the extent of Glutamic Pyruvic transaminase activity to be higher than that of Glutamic Oxalacetic transminase activity. A general decline in transaminases activity as a function of sublethal concentration of heavy metal chromium was evidenced which reflects the injury to lysosome due to stressing the biological system. Dose as well as exposure period dependent statistically significant (P<0.05) inhibition in Glutamic Oxalacetic transminase activity in liver and brain and Glutamic Pyruvic transaminase activity in liver and kidney of Catla catla are advocated as bioindicators of water borne heavy metal chromium stress.
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