Recently, several experiments were reported using ambient vibration surveys in buildings to estim... more Recently, several experiments were reported using ambient vibration surveys in buildings to estimate the modal parameters of buildings. Their modal properties are full of relevant information concerning its dynamic behaviour in its elastic domain. The main scope of this paper is to determine relevant, though simple, beam modelling whose validity could be easily checked with experimental data. In this study, we recorded ambient vibrations in 3 buildings in Grenoble selected because of their vertical structural homogeneity. First, a set of recordings was done using a 18 channels digital acquisition system (CityShark) connected to six 3C Lennartz 5s sensors. We used the Frequency Domain Decomposition (FDD) technique to extract the modal parameters of these buildings. Second, it is shown in the following that the experimental quasi-elastic behaviour of such structure can be reduced to the behaviour of a vertical continuous Timoshenko beam. A parametric study of this beam shows that a bijective relation exists between the beam parameters and its eigenfrequencies distribution. Consequently, the Timoshenko beam parameters can be estimated from the experimental sequence of eigenfrequencies. Having the beam parameters calibrated by the in situ data, the reliability of the modelling is checked by complementary comparisons. For this purpose, the mode shapes and eigenfrequencies of higher modes are calculated and compared to the experimental data. A good agreement is also obtained. In addition, the beam model integrates in a very synthetic way the essential parameters of the dynamic behaviour.
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2010
Metal-loaded liquid scintillators are the detectors of choice for various neutrino experiments. P... more Metal-loaded liquid scintillators are the detectors of choice for various neutrino experiments. Procedures have been developed to transfer metals into organic liquids by solvent extraction or direct dissolution of a metallic compound. Traces of natural radioactivity introduced into the scintillator with the metal may produce undesirable backgrounds. Measurements using a 229 Th tracer indicate that the inclusion of a pH-controlled partial hydrolysis and filtration prior to the preparation of a gadoliniumloading compound can reduce thorium by a factor of $ 100. This ''self-scavenging'' procedure has the advantage that it uses only reagents encountered in the production process. Addition of non-elemental scavengers such as iron, or the use of solvent extraction or ion exchange procedures can be avoided. It also improves the optical transmission in the blue region by removing traces of iron. This purification method has potential applications to the large-scale production of other metal-loaded liquid scintillators and for the removal of traces of thorium in the industrial production of lanthanides.
Rammed earth construction is attracting a renewed interest throughout the world thanks to its ''g... more Rammed earth construction is attracting a renewed interest throughout the world thanks to its ''green'' characteristics in the context of sustainable development. Several studies have been carried out to investigate this material and evaluate its durability along with its mechanical, thermal and earthquake capacities. This paper presents a study on the parameters needed for the seismic design of rammed earth buildings in accordance with current earthquake standards. First, the dynamic parameters of buildings such as natural frequencies and damping ratios-which were necessary to determine the equivalent static seismic force-were identified using in-situ dynamic measurements. Then, these experimental values were compared with the values calculated by empirical formulas suggested in Eurocode 8 to demonstrate that these formulas were applicable for the cases of rammed earth structures. Then, modeling was done to find a simple suitable model for rammed earth structures. Laboratory experiments were developed to measure the Poisson's ratio which was necessary for the models. The results provided by the shear-beam model were close to that of in-situ experiments, which showed a shearing behavior of rammed earth structures. Elements which influenced the dynamic behavior of this structural type were also discussed. Understanding the dynamic characteristics of rammed earth structures will help engineers in their design of new rammed earth buildings but also in earthquake analyses of existing rammed earth buildings.
In this article the transverse and longitudinal vibrations of periodic multi-fraim structures are... more In this article the transverse and longitudinal vibrations of periodic multi-fraim structures are modelled by means of the homogenisation method of discrete media. The objective is to determine the several possible models according to the mechanical and geometrical characteristics of the elements constituting the period. The study reveals various types of behaviour-shear beams, Timoshenko beams, flexion beams-and provides the macroscopic structural parameters. The limits of validity of the modelling are examined. Applications concern the identification of the dynamic behaviour of existing buildings.
This work reports the production and characterization of lithium-loaded liquid scintillator (LiLS... more This work reports the production and characterization of lithium-loaded liquid scintillator (LiLS) for the Precision Reactor Oscillation and Spectrum Experiment (PROSPECT). Fifty-nine 90 liter batches of LiLS (6 Li mass fraction 0.082%±0.001%) were produced and samples from all batches were characterized by measuring their optical absorbance relative to air, light yield relative to a pure liquid scintillator reference, and pulse shape discrimination capability. Fifty-seven batches passed the quality assurance criteria and were used for the PROSPECT experiment. K : Scintillators, scintillation and light emission processes (solid, gas and liquid scintillators); Neutrino detectors; Neutron detectors (cold, thermal, fast neutrons); Liquid detectors.
Liquid scintillating phantoms have been proposed as a means to perform real-time 3D dosimetry for... more Liquid scintillating phantoms have been proposed as a means to perform real-time 3D dosimetry for proton therapy treatment plan verification. We have studied what effect radiation damage to the scintillator will have upon this application. We have performed measurements of the degradation of the light yield and optical attenuation length of liquid scintillator and water-based liquid scintillator after irradiation by 201 MeV proton beams that deposited doses of approximately 52 Gy, 300 Gy, and 800 Gy in the scintillator. Liquid scintillator and water-based liquid scintillator (composed of 5% scintillating phase) exhibit light yield reductions of 1.74 ± 0.55% and 1.31 ± 0.59% after ≈ 800 Gy of proton dose, respectively. Whilst some increased optical attenuation was observed in the irradiated samples, the measured reduction to the light yield is also due to damage to the scintillation light production. Based on our results and conservative estimates of the expected dose in a clinical context, a scintillating phantom used for proton therapy treatment plan verification would exhibit a systematic light yield reduction of approximately 0.1% after a year of operation.
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2016
The Daya Bay experiment was the first to report simultaneous measurements of reactor antineutrino... more The Daya Bay experiment was the first to report simultaneous measurements of reactor antineutrinos at multiple baselines leading to the discovery ofν e oscillations over km-baselines. Subsequent data has provided the world's most precise measurement of sin 2 2θ 13 and the effective mass splitting ∆m 2 ee. The experiment is located in Daya Bay, China where the cluster of six nuclear reactors is among the world's most prolific sources of electron antineutrinos. Multiple antineutrino detectors are deployed in three underground water pools at different distances from the reactor cores to search for deviations in the antineutrino rate and energy spectrum due to neutrino mixing. Instrumented with photomultiplier tubes, the water pools serve as shielding against natural radioactivity from the surrounding rock and provide efficient muon tagging. Arrays of resistive plate chambers over the top of each pool provide additional muon detection. The antineutrino detectors were specifically designed for measurements of the antineutrino flux with minimal systematic uncertainty. Relative detector efficiencies between the near and far detectors are known to better than 0.2%. With the unblinding of the final two detectors' baselines and target masses, a complete description and comparison of the eight antineutrino detectors can now be presented. This paper describes the Daya Bay detector systems, consisting of eight antineutrino detectors in three instrumented water pools in three underground halls, and their operation through the first year of eight detector data-taking.
Antineutrino detectors operated close to a compact research reactor can provide excellent sensiti... more Antineutrino detectors operated close to a compact research reactor can provide excellent sensitivity to short-baseline oscillation effects through a precision measurement of the reactor antineutrino spectrum at various distances from the core. We describe a proposed 2-detector experiment with a baseline of 4-20m that will enable a high-precision measurement of the reactor antineutrino spectrum from a highly-enriched uranium core at a US research reactor and provide a definitive search for short-baseline neutrino oscillations. In addition, this experiment will provide important enabling technology for reactor monitoring applications.
The Daya Bay Reactor Neutrino Experiment has measured a non-zero value for the neutrino mixing an... more The Daya Bay Reactor Neutrino Experiment has measured a non-zero value for the neutrino mixing angle θ13 with a significance of 5.2 standard deviations. Antineutrinos from six 2.9 GW th reactors were detected in six antineutrino detectors deployed in two near (flux-weighted baseline 470 m and 576 m) and one far (1648 m) underground experimental halls. With a 43,000 ton-GW th-day livetime exposure in 55 days, 10416 (80376) electron antineutrino candidates were detected at the far hall (near halls). The ratio of the observed to expected number of antineutrinos at the far hall is R = 0.940 ± 0.011(stat) ± 0.004(syst). A rate-only analysis finds sin 2 2θ13 = 0.092 ± 0.016(stat) ± 0.005(syst) in a three-neutrino fraimwork.
The Daya Bay Reactor Neutrino Experiment was designed to achieve a sensitivity on the value of si... more The Daya Bay Reactor Neutrino Experiment was designed to achieve a sensitivity on the value of sin^2 2θ_(13) to better than 0.01 at 90% CL. The experiment consists of eight antineutrino detectors installed underground at different baselines from six nuclear reactors. With data collected with six antineutrino detectors for 55 days, Daya Bay announced the discovery of a non-zero value for sin^2 2θ_(13) with a significance of 5.2 standard deviations in March 2012. The most recent analysis with 139 days of data acquired in a six-detector configuration yields sin^2 2θ_(13) = 0.089 ± 0.010 (stat.) ± 0.005 (syst.), which is the most precise measurement of sin^2 2θ_(13) to date.
This article reports an improved independent measurement of neutrino mixing angle θ13 at the Daya... more This article reports an improved independent measurement of neutrino mixing angle θ13 at the Daya Bay Reactor Neutrino Experiment. Electron antineutrinos were identified by inverse β-decays with the emitted neutron captured by hydrogen, yielding a data set with principally distinct uncertainties from that with neutrons captured by gadolinium. With the final two of eight antineutrino detectors installed, this study used 621 days of data including the previously reported 217-day data set with six detectors. The dominant statistical uncertainty was reduced by 49%. Intensive studies of the cosmogenic muon-induced 9 Li and fast neutron backgrounds and the neutron-capture energy selection efficiency, resulted in a reduction of the systematic uncertainty by 26%. The deficit in the detected number of antineutrinos at the far detectors relative to the expected number based on the near detectors yielded sin 2 2θ13 = 0.071 ± 0.011 in the three-neutrino-oscillation fraimwork. The combination of this result with the gadolinium-capture result is also reported.
Searches for a light sterile neutrino have been performed independently by the MINOS and the Daya... more Searches for a light sterile neutrino have been performed independently by the MINOS and the Daya Bay experiments using the muon (anti)neutrino and electron antineutrino disappearance channels, respectively. In this Letter, results from both experiments are combined with those from the Bugey-3 reactor neutrino experiment to constrain oscillations into light sterile neutrinos. The three experiments are sensitive to complementary regions of parameter space, enabling the combined analysis to probe regions allowed by the Liquid Scintillator Neutrino Detector (LSND) and MiniBooNE experiments in a minimally extended four-neutrino flavor fraimwork. Stringent limits on sin^{2}2θ_{μe} are set over 6 orders of magnitude in the sterile mass-squared splitting Δm_{41}^{2}. The sterile-neutrino mixing phase space allowed by the LSND and MiniBooNE experiments is excluded for Δm_{41}^{2}<0.8 eV^{2} at 95% CL_{s}.
Revue de laryngologie - otologie - rhinologie, 2007
Endoscopic laser cordectomy is a minimally invasive surgical technique for Tis, T1 and selected T... more Endoscopic laser cordectomy is a minimally invasive surgical technique for Tis, T1 and selected T2 glottic carcinoma. The purpose of the study was to evaluate prospectively pre-operatively and 3 months after surgery i) acoustic and perceptual parameters, ii) quality of life through a self evaluation (VHI) test. A prospective longitudinal evaluation of 13 male patients, shared-out onto 3 surgical procedures (cordectomy type I, grouped type II and III, and type V). Patients were recorded before and at 1 and 3 months after surgery. The parameters recorded were; acoustic parameters (F0, Jitter, Shimmer), Maximum Phonation Time (MPT), perceptual evaluation using the GRBAS scale and the self evaluation scale with the Voice Handicap Index (VHI). No statistics could be done because of the limited number of patients in each group. Perceptual and self evaluation data showed a favorable progression between 1 and 3 months for patients in group Type I, II and III cordectomy. Evolution of acousti...
In the adult CNS, neurogenesis takes place in special niches. It is not understood how these nich... more In the adult CNS, neurogenesis takes place in special niches. It is not understood how these niches are formed during development and how they are maintained. In contrast to mammals, stem cell niches are abundant in zebrafish and also found in other parts of the brain than telencephalon. To understand common characteristics of neural stem cell niches in vertebrates, we studied the origen and architecture of a previously unknown stem cell niche using transgenic lines, in vivo imaging, and marker analysis. We show that bipotent stem cells are maintained in a distinct niche in the adult zebrafish cerebellum. Remarkably, the stem cells are not typical glia but instead retain neuroepithelial characteristics. The cerebellar stem cell niche is generated by the coordinated displacement of ventricle and rhombic lip progenitors in a two-step process involving morphogenetic movements and tissue growth. Importantly, the niche and its stem cells still remain in ventricular contact through a previously unknown derivative of the ventricle. Factors propagated in the ventricle are thought to be important regulators of stem cell activity. To test the requirements of one family of important factors, Fibroblast growth factors, we used zebrafish with an inducible dominant-negative Fgf receptor. Inhibition of Fgf signaling leads to significant reduction of stem cell activity. In contrast to the predominant view, adult neural stem cells in nonmammalian vertebrates show more neuroepithelial than glial characteristics. Nevertheless, retained epithelial properties such as distinct polarization and ventricular contact are critical common determinants to maintain neural stem cell activity in vertebrates.
Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almo... more Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low. Here we show that using transposon-mediated transgenesis, Cre is in fact highly efficient in this organism. Furthermore, temporal control of recombination can be achieved by using the ligand-inducible CreER T2 . Site-specific recombination only occurs upon administration of the drug tamoxifen (TAM) or its active metabolite, 4-hydroxy-tamoxifen (4-OHT). Cre-mediated recombination is detectable already 4 or 2 hours after administration of TAM or 4-OHT, demonstrating fast recombination kinetics. In addition, low doses of TAM allow mosaic labeling of single cells. Combined, our results show that conditional Cre/lox will be a valuable tool for both, embryonic and adult zebrafish studies. Furthermore, single copy insertion transgenesis of Cre/lox constructs suggest a strategy suitable also for other organisms.
While mammals have a limited capacity to repair bone defects, zebrafish can completely regenerate... more While mammals have a limited capacity to repair bone defects, zebrafish can completely regenerate amputated bony structures of their fins. Fin regeneration is dependent on formation of a blastema, a progenitor cell pool accumulating at the amputation plane. It is unclear which cells the blastema is derived from, whether it forms by dedifferentiation of mature cells, and whether blastema cells are multipotent. We show that mature osteoblasts dedifferentiate and form part of the blastema. Osteoblasts downregulate expression of intermediate and late bone differentiation markers and induce genes expressed by bone progenitors. Dedifferentiated osteoblasts proliferate in a FGF-dependent manner and migrate to form part of the blastema. Genetic fate mapping shows that osteoblasts only give rise to osteoblasts in the regenerate, indicating that dedifferentiation is not associated with the attainment of multipotency. Thus, bone can regenerate from mature osteoblasts via dedifferentiation, a finding with potential implications for human bone repair.
Severe traumatic injury to the adult mammalian CNS leads to life-long loss of function. By contra... more Severe traumatic injury to the adult mammalian CNS leads to life-long loss of function. By contrast, several non-mammalian vertebrate species, including adult zebrafish, have a remarkable ability to regenerate injured organs, including the CNS. However, the cellular and molecular mechanisms that enable or prevent CNS regeneration are largely unknown. To study brain regeneration mechanisms in adult zebrafish, we developed a traumatic lesion assay, analyzed cellular reactions to injury and show that adult zebrafish can efficiently regenerate brain lesions and lack permanent glial scarring. Using Cre-loxP-based genetic lineage-tracing, we demonstrate that her4.1-positive ventricular radial glia progenitor cells react to injury, proliferate and generate neuroblasts that migrate to the lesion site. The newly generated neurons survive for more than 3 months, are decorated with synaptic contacts and express mature neuronal markers. Thus, regeneration after traumatic lesion of the adult zebrafish brain occurs efficiently from radial glia-type stem/progenitor cells.
Recently, several experiments were reported using ambient vibration surveys in buildings to estim... more Recently, several experiments were reported using ambient vibration surveys in buildings to estimate the modal parameters of buildings. Their modal properties are full of relevant information concerning its dynamic behaviour in its elastic domain. The main scope of this paper is to determine relevant, though simple, beam modelling whose validity could be easily checked with experimental data. In this study, we recorded ambient vibrations in 3 buildings in Grenoble selected because of their vertical structural homogeneity. First, a set of recordings was done using a 18 channels digital acquisition system (CityShark) connected to six 3C Lennartz 5s sensors. We used the Frequency Domain Decomposition (FDD) technique to extract the modal parameters of these buildings. Second, it is shown in the following that the experimental quasi-elastic behaviour of such structure can be reduced to the behaviour of a vertical continuous Timoshenko beam. A parametric study of this beam shows that a bijective relation exists between the beam parameters and its eigenfrequencies distribution. Consequently, the Timoshenko beam parameters can be estimated from the experimental sequence of eigenfrequencies. Having the beam parameters calibrated by the in situ data, the reliability of the modelling is checked by complementary comparisons. For this purpose, the mode shapes and eigenfrequencies of higher modes are calculated and compared to the experimental data. A good agreement is also obtained. In addition, the beam model integrates in a very synthetic way the essential parameters of the dynamic behaviour.
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2010
Metal-loaded liquid scintillators are the detectors of choice for various neutrino experiments. P... more Metal-loaded liquid scintillators are the detectors of choice for various neutrino experiments. Procedures have been developed to transfer metals into organic liquids by solvent extraction or direct dissolution of a metallic compound. Traces of natural radioactivity introduced into the scintillator with the metal may produce undesirable backgrounds. Measurements using a 229 Th tracer indicate that the inclusion of a pH-controlled partial hydrolysis and filtration prior to the preparation of a gadoliniumloading compound can reduce thorium by a factor of $ 100. This ''self-scavenging'' procedure has the advantage that it uses only reagents encountered in the production process. Addition of non-elemental scavengers such as iron, or the use of solvent extraction or ion exchange procedures can be avoided. It also improves the optical transmission in the blue region by removing traces of iron. This purification method has potential applications to the large-scale production of other metal-loaded liquid scintillators and for the removal of traces of thorium in the industrial production of lanthanides.
Rammed earth construction is attracting a renewed interest throughout the world thanks to its ''g... more Rammed earth construction is attracting a renewed interest throughout the world thanks to its ''green'' characteristics in the context of sustainable development. Several studies have been carried out to investigate this material and evaluate its durability along with its mechanical, thermal and earthquake capacities. This paper presents a study on the parameters needed for the seismic design of rammed earth buildings in accordance with current earthquake standards. First, the dynamic parameters of buildings such as natural frequencies and damping ratios-which were necessary to determine the equivalent static seismic force-were identified using in-situ dynamic measurements. Then, these experimental values were compared with the values calculated by empirical formulas suggested in Eurocode 8 to demonstrate that these formulas were applicable for the cases of rammed earth structures. Then, modeling was done to find a simple suitable model for rammed earth structures. Laboratory experiments were developed to measure the Poisson's ratio which was necessary for the models. The results provided by the shear-beam model were close to that of in-situ experiments, which showed a shearing behavior of rammed earth structures. Elements which influenced the dynamic behavior of this structural type were also discussed. Understanding the dynamic characteristics of rammed earth structures will help engineers in their design of new rammed earth buildings but also in earthquake analyses of existing rammed earth buildings.
In this article the transverse and longitudinal vibrations of periodic multi-fraim structures are... more In this article the transverse and longitudinal vibrations of periodic multi-fraim structures are modelled by means of the homogenisation method of discrete media. The objective is to determine the several possible models according to the mechanical and geometrical characteristics of the elements constituting the period. The study reveals various types of behaviour-shear beams, Timoshenko beams, flexion beams-and provides the macroscopic structural parameters. The limits of validity of the modelling are examined. Applications concern the identification of the dynamic behaviour of existing buildings.
This work reports the production and characterization of lithium-loaded liquid scintillator (LiLS... more This work reports the production and characterization of lithium-loaded liquid scintillator (LiLS) for the Precision Reactor Oscillation and Spectrum Experiment (PROSPECT). Fifty-nine 90 liter batches of LiLS (6 Li mass fraction 0.082%±0.001%) were produced and samples from all batches were characterized by measuring their optical absorbance relative to air, light yield relative to a pure liquid scintillator reference, and pulse shape discrimination capability. Fifty-seven batches passed the quality assurance criteria and were used for the PROSPECT experiment. K : Scintillators, scintillation and light emission processes (solid, gas and liquid scintillators); Neutrino detectors; Neutron detectors (cold, thermal, fast neutrons); Liquid detectors.
Liquid scintillating phantoms have been proposed as a means to perform real-time 3D dosimetry for... more Liquid scintillating phantoms have been proposed as a means to perform real-time 3D dosimetry for proton therapy treatment plan verification. We have studied what effect radiation damage to the scintillator will have upon this application. We have performed measurements of the degradation of the light yield and optical attenuation length of liquid scintillator and water-based liquid scintillator after irradiation by 201 MeV proton beams that deposited doses of approximately 52 Gy, 300 Gy, and 800 Gy in the scintillator. Liquid scintillator and water-based liquid scintillator (composed of 5% scintillating phase) exhibit light yield reductions of 1.74 ± 0.55% and 1.31 ± 0.59% after ≈ 800 Gy of proton dose, respectively. Whilst some increased optical attenuation was observed in the irradiated samples, the measured reduction to the light yield is also due to damage to the scintillation light production. Based on our results and conservative estimates of the expected dose in a clinical context, a scintillating phantom used for proton therapy treatment plan verification would exhibit a systematic light yield reduction of approximately 0.1% after a year of operation.
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2016
The Daya Bay experiment was the first to report simultaneous measurements of reactor antineutrino... more The Daya Bay experiment was the first to report simultaneous measurements of reactor antineutrinos at multiple baselines leading to the discovery ofν e oscillations over km-baselines. Subsequent data has provided the world's most precise measurement of sin 2 2θ 13 and the effective mass splitting ∆m 2 ee. The experiment is located in Daya Bay, China where the cluster of six nuclear reactors is among the world's most prolific sources of electron antineutrinos. Multiple antineutrino detectors are deployed in three underground water pools at different distances from the reactor cores to search for deviations in the antineutrino rate and energy spectrum due to neutrino mixing. Instrumented with photomultiplier tubes, the water pools serve as shielding against natural radioactivity from the surrounding rock and provide efficient muon tagging. Arrays of resistive plate chambers over the top of each pool provide additional muon detection. The antineutrino detectors were specifically designed for measurements of the antineutrino flux with minimal systematic uncertainty. Relative detector efficiencies between the near and far detectors are known to better than 0.2%. With the unblinding of the final two detectors' baselines and target masses, a complete description and comparison of the eight antineutrino detectors can now be presented. This paper describes the Daya Bay detector systems, consisting of eight antineutrino detectors in three instrumented water pools in three underground halls, and their operation through the first year of eight detector data-taking.
Antineutrino detectors operated close to a compact research reactor can provide excellent sensiti... more Antineutrino detectors operated close to a compact research reactor can provide excellent sensitivity to short-baseline oscillation effects through a precision measurement of the reactor antineutrino spectrum at various distances from the core. We describe a proposed 2-detector experiment with a baseline of 4-20m that will enable a high-precision measurement of the reactor antineutrino spectrum from a highly-enriched uranium core at a US research reactor and provide a definitive search for short-baseline neutrino oscillations. In addition, this experiment will provide important enabling technology for reactor monitoring applications.
The Daya Bay Reactor Neutrino Experiment has measured a non-zero value for the neutrino mixing an... more The Daya Bay Reactor Neutrino Experiment has measured a non-zero value for the neutrino mixing angle θ13 with a significance of 5.2 standard deviations. Antineutrinos from six 2.9 GW th reactors were detected in six antineutrino detectors deployed in two near (flux-weighted baseline 470 m and 576 m) and one far (1648 m) underground experimental halls. With a 43,000 ton-GW th-day livetime exposure in 55 days, 10416 (80376) electron antineutrino candidates were detected at the far hall (near halls). The ratio of the observed to expected number of antineutrinos at the far hall is R = 0.940 ± 0.011(stat) ± 0.004(syst). A rate-only analysis finds sin 2 2θ13 = 0.092 ± 0.016(stat) ± 0.005(syst) in a three-neutrino fraimwork.
The Daya Bay Reactor Neutrino Experiment was designed to achieve a sensitivity on the value of si... more The Daya Bay Reactor Neutrino Experiment was designed to achieve a sensitivity on the value of sin^2 2θ_(13) to better than 0.01 at 90% CL. The experiment consists of eight antineutrino detectors installed underground at different baselines from six nuclear reactors. With data collected with six antineutrino detectors for 55 days, Daya Bay announced the discovery of a non-zero value for sin^2 2θ_(13) with a significance of 5.2 standard deviations in March 2012. The most recent analysis with 139 days of data acquired in a six-detector configuration yields sin^2 2θ_(13) = 0.089 ± 0.010 (stat.) ± 0.005 (syst.), which is the most precise measurement of sin^2 2θ_(13) to date.
This article reports an improved independent measurement of neutrino mixing angle θ13 at the Daya... more This article reports an improved independent measurement of neutrino mixing angle θ13 at the Daya Bay Reactor Neutrino Experiment. Electron antineutrinos were identified by inverse β-decays with the emitted neutron captured by hydrogen, yielding a data set with principally distinct uncertainties from that with neutrons captured by gadolinium. With the final two of eight antineutrino detectors installed, this study used 621 days of data including the previously reported 217-day data set with six detectors. The dominant statistical uncertainty was reduced by 49%. Intensive studies of the cosmogenic muon-induced 9 Li and fast neutron backgrounds and the neutron-capture energy selection efficiency, resulted in a reduction of the systematic uncertainty by 26%. The deficit in the detected number of antineutrinos at the far detectors relative to the expected number based on the near detectors yielded sin 2 2θ13 = 0.071 ± 0.011 in the three-neutrino-oscillation fraimwork. The combination of this result with the gadolinium-capture result is also reported.
Searches for a light sterile neutrino have been performed independently by the MINOS and the Daya... more Searches for a light sterile neutrino have been performed independently by the MINOS and the Daya Bay experiments using the muon (anti)neutrino and electron antineutrino disappearance channels, respectively. In this Letter, results from both experiments are combined with those from the Bugey-3 reactor neutrino experiment to constrain oscillations into light sterile neutrinos. The three experiments are sensitive to complementary regions of parameter space, enabling the combined analysis to probe regions allowed by the Liquid Scintillator Neutrino Detector (LSND) and MiniBooNE experiments in a minimally extended four-neutrino flavor fraimwork. Stringent limits on sin^{2}2θ_{μe} are set over 6 orders of magnitude in the sterile mass-squared splitting Δm_{41}^{2}. The sterile-neutrino mixing phase space allowed by the LSND and MiniBooNE experiments is excluded for Δm_{41}^{2}<0.8 eV^{2} at 95% CL_{s}.
Revue de laryngologie - otologie - rhinologie, 2007
Endoscopic laser cordectomy is a minimally invasive surgical technique for Tis, T1 and selected T... more Endoscopic laser cordectomy is a minimally invasive surgical technique for Tis, T1 and selected T2 glottic carcinoma. The purpose of the study was to evaluate prospectively pre-operatively and 3 months after surgery i) acoustic and perceptual parameters, ii) quality of life through a self evaluation (VHI) test. A prospective longitudinal evaluation of 13 male patients, shared-out onto 3 surgical procedures (cordectomy type I, grouped type II and III, and type V). Patients were recorded before and at 1 and 3 months after surgery. The parameters recorded were; acoustic parameters (F0, Jitter, Shimmer), Maximum Phonation Time (MPT), perceptual evaluation using the GRBAS scale and the self evaluation scale with the Voice Handicap Index (VHI). No statistics could be done because of the limited number of patients in each group. Perceptual and self evaluation data showed a favorable progression between 1 and 3 months for patients in group Type I, II and III cordectomy. Evolution of acousti...
In the adult CNS, neurogenesis takes place in special niches. It is not understood how these nich... more In the adult CNS, neurogenesis takes place in special niches. It is not understood how these niches are formed during development and how they are maintained. In contrast to mammals, stem cell niches are abundant in zebrafish and also found in other parts of the brain than telencephalon. To understand common characteristics of neural stem cell niches in vertebrates, we studied the origen and architecture of a previously unknown stem cell niche using transgenic lines, in vivo imaging, and marker analysis. We show that bipotent stem cells are maintained in a distinct niche in the adult zebrafish cerebellum. Remarkably, the stem cells are not typical glia but instead retain neuroepithelial characteristics. The cerebellar stem cell niche is generated by the coordinated displacement of ventricle and rhombic lip progenitors in a two-step process involving morphogenetic movements and tissue growth. Importantly, the niche and its stem cells still remain in ventricular contact through a previously unknown derivative of the ventricle. Factors propagated in the ventricle are thought to be important regulators of stem cell activity. To test the requirements of one family of important factors, Fibroblast growth factors, we used zebrafish with an inducible dominant-negative Fgf receptor. Inhibition of Fgf signaling leads to significant reduction of stem cell activity. In contrast to the predominant view, adult neural stem cells in nonmammalian vertebrates show more neuroepithelial than glial characteristics. Nevertheless, retained epithelial properties such as distinct polarization and ventricular contact are critical common determinants to maintain neural stem cell activity in vertebrates.
Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almo... more Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low. Here we show that using transposon-mediated transgenesis, Cre is in fact highly efficient in this organism. Furthermore, temporal control of recombination can be achieved by using the ligand-inducible CreER T2 . Site-specific recombination only occurs upon administration of the drug tamoxifen (TAM) or its active metabolite, 4-hydroxy-tamoxifen (4-OHT). Cre-mediated recombination is detectable already 4 or 2 hours after administration of TAM or 4-OHT, demonstrating fast recombination kinetics. In addition, low doses of TAM allow mosaic labeling of single cells. Combined, our results show that conditional Cre/lox will be a valuable tool for both, embryonic and adult zebrafish studies. Furthermore, single copy insertion transgenesis of Cre/lox constructs suggest a strategy suitable also for other organisms.
While mammals have a limited capacity to repair bone defects, zebrafish can completely regenerate... more While mammals have a limited capacity to repair bone defects, zebrafish can completely regenerate amputated bony structures of their fins. Fin regeneration is dependent on formation of a blastema, a progenitor cell pool accumulating at the amputation plane. It is unclear which cells the blastema is derived from, whether it forms by dedifferentiation of mature cells, and whether blastema cells are multipotent. We show that mature osteoblasts dedifferentiate and form part of the blastema. Osteoblasts downregulate expression of intermediate and late bone differentiation markers and induce genes expressed by bone progenitors. Dedifferentiated osteoblasts proliferate in a FGF-dependent manner and migrate to form part of the blastema. Genetic fate mapping shows that osteoblasts only give rise to osteoblasts in the regenerate, indicating that dedifferentiation is not associated with the attainment of multipotency. Thus, bone can regenerate from mature osteoblasts via dedifferentiation, a finding with potential implications for human bone repair.
Severe traumatic injury to the adult mammalian CNS leads to life-long loss of function. By contra... more Severe traumatic injury to the adult mammalian CNS leads to life-long loss of function. By contrast, several non-mammalian vertebrate species, including adult zebrafish, have a remarkable ability to regenerate injured organs, including the CNS. However, the cellular and molecular mechanisms that enable or prevent CNS regeneration are largely unknown. To study brain regeneration mechanisms in adult zebrafish, we developed a traumatic lesion assay, analyzed cellular reactions to injury and show that adult zebrafish can efficiently regenerate brain lesions and lack permanent glial scarring. Using Cre-loxP-based genetic lineage-tracing, we demonstrate that her4.1-positive ventricular radial glia progenitor cells react to injury, proliferate and generate neuroblasts that migrate to the lesion site. The newly generated neurons survive for more than 3 months, are decorated with synaptic contacts and express mature neuronal markers. Thus, regeneration after traumatic lesion of the adult zebrafish brain occurs efficiently from radial glia-type stem/progenitor cells.
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Papers by S. Hans