Papers by Prof. Dr. Kazi Shakhawath Hossain
Journal of entomology and zoology studies, 2019
The larvae and pupae of different mosquito species were counted; water was preserved for fungal c... more The larvae and pupae of different mosquito species were counted; water was preserved for fungal culture. Potato Dextrose Agar (PDA) medium was used for culture and different levels of dilution were performed for culture of fungus spores. These stagnant dirty drain water samples have been containing a few (10) Aedes larvae; coexisting with a huge Culex at the same breeding ground. A total of 4138 mosquito larvae and pupa were recorded in the collected habitat water where 3928 larvae and 200 pupa of Culex were observed with different larval densities among collection points. Out of eighteen (18) fungal isolates under 10 genera (Absidia sp., Aspergillus sp., Cladosporium sp., Curvularia sp., Fusarium sp., Geotrichum sp., Nigrospora sp., Penicillium sp., Rhizopus sp. and Sclerotium sp.) were identified, seven (7) of them have been reported as entomopathogenic by scientists; to date. Eight (8) isolates were belonging to the genus Aspergillus. Relationship of larval density of Culex were very weak to total spores of all fungi (r =-0.154) as well as with the number of individual isolates of fungus spores. Culture of entomopathogenic fungi in laboratory condition, and use of them as a biological control agent for the mosquitoes could not be recommended.
Dhaka University Journal of Biological Sciences, Jun 30, 2015
Antifungal potentials of nine fungicides and eight plant parts extracts were tested in opposition... more Antifungal potentials of nine fungicides and eight plant parts extracts were tested in opposition to Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc.the two pathogens, isolated from wilted roots of brinjal plants. Efficiency gradients observed in the present study expressed that Bavistin and Tall were the best inhibiting agent against the in vitro growth of the test pathogenic fungi. Among aqueous extracts of eight plant parts significant inhibition of the growth of the pathogens was observed with Azadiracta indica (leaf), Zingiber officinale (rhizome) and Asparagus racemosus (root) at 20% concentration.
Bangladesh Journal of Botany, 2021
Isolation and identification of mycorrhizal fungi from the roots of Rhynchostylis retusa indicate... more Isolation and identification of mycorrhizal fungi from the roots of Rhynchostylis retusa indicated that the cultural and microscopic features, namely colony appearance, colony colour, diameter of vegetative hyphae, presence of monilioid cells, right angle branching pattern of the fungal endophyte corroborated the identity of the fungus Rhizoctonia like anamorphs of Ceratobasidium species. Fungal identity was further confirmed through sequencing and analysis of internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA (nrDNA). ITS sequence of the isolate RhY10A6 (accession no. MN120903.1) showed > 99% similarity with several isolates of the teleomorphic fungus Ceratobasidium sp. in NCBI mega blast search. The phylogenetic analysis based on maximum parsimony method, this orchid mycorrhizal fungus clustered with several isolates of Ceratobasidium sp. or Rhizoctonia like fungi. It showed near distant relation with Ceratobasidium ramicola (GeneBank accession no. NR_1383...
Bangladesh Journal of Botany, 2020
Silver nanoparticles (Ag-NPs) by mixing silver nitrate (AgNO3) with cell-free filtrate (CFF) of t... more Silver nanoparticles (Ag-NPs) by mixing silver nitrate (AgNO3) with cell-free filtrate (CFF) of the two fungal isolates viz., Fusarium 4F1 and Trichoderma TrS were synthesized. pH, substrate concentration and incubation period for the production of better quality and quantity of Ag-NPs was optimized. The Ag-NPs by UV-vis spectroscopy were characterized. Between the two fungal isolates, pH levels, AgNO3 concentrations and incubation periods studied, the highest number of spherical shaped, monodispersed and stable Ag-NPs were recorded from Fusarium 4F1 at pH 9, 2 mM AgNO3 and 72 hrs of incubation.
Rice (Oryza sativa L.) is the principal food crops in Bangladesh. Here different rice varieties a... more Rice (Oryza sativa L.) is the principal food crops in Bangladesh. Here different rice varieties are grown in 3 rice growing seasons per year viz. Aus, Aman and Boro. Eleven rice varieties were grown at randomly sampled 50 farmers’ plots in Chandiara village during Boro (November, 2007 – May, 2008), Aus (April – July, 2008) and T. Aman (July – December, 2008) seasons. Among the varieties farmers were preferred BRRI dhan 28 mostly during the Boro and Aus seasons. During the T. Aman season it was BRRI dhan 32. All the farmers’ plots were seeded with BRRI released varieties during the T. Aman season though it was 90 and 82% during the Aus and Boro seasons respectively. In these 3 seasons most of the farmers did not procure certified seeds of BADC or non-government agencies. 62 to 88% rice plots per season were seeded with farmer saved seeds in the sampled plots. Bakanae disease incidence was much higher in these plots and comparatively less frequent in the plots seeded with certified se...
Bangladesh Journal of Plant Taxonomy, 2016
From three farming stages of Arthrospira platensis (Nordstedt) Gomont [Spirulina platensis (Gomon... more From three farming stages of Arthrospira platensis (Nordstedt) Gomont [Spirulina platensis (Gomont) Geitler], seven fungal species, namely, Cladosporium varians Braun, Melnik & K. Schub., Fusarium trichothecioides Wollenw., Geotrichum candidum Link, Mucor circinelloides Tiegh., M. hiemalis Wehmer, Penicillium frequentans Westling and Verticillium albo-atrum Reinke & Berthold were recorded for the first time from Bangladesh.
IMC Journal of Medical Science, 2020
Background and objectives: Candida auris is an emerging multidrug-resistant fungal pathogen that ... more Background and objectives: Candida auris is an emerging multidrug-resistant fungal pathogen that has been associated with nosocomial infections with a high mortality. The organism has been reported from several countries of the world except Bangladesh. The present study describes the presence of C. auris in clinical samples obtained from a large hospital of Dhaka city, Bangladesh. Materials and methods: The A total of 100 Candida species isolated from different clinical samples were purposively included in the present study. Samples were obtained from patients attending a 750 bed hospital of Dhaka city. C. auris was identified by growth characteristics, biochemical and carbohydrate assimilation test and further confirmed by polymerase chain reaction and sequencing using ITS1 and ITS2 targeting the conserved regions of 5.8S rRNA. Antifungal susceptibility of identified C. auris was performed by disk diffusion and minimum inhibitory concentration (MIC) methods. Results: Out of 100 Can...
Bangladesh Journal of Botany, 2018
Bakanae disease is widely distributed in all rice growing areas of the world. In Bangladesh, it i... more Bakanae disease is widely distributed in all rice growing areas of the world. In Bangladesh, it is one of the major diseases of rice. Four methods were tested for management of this disease, of which, roguing proved ineffective in a field investigation. Among the 15 fungicides tested in vitro, all of them were found effective in various degrees against the pathogen of the disease. Best effective 4 were selected for seed treatment and know (50% carbendazim WP) was the most effective followed by folicur (25% Tebuconazole EC), protaf (25% Propiconazole EC) and celest extra (2.5% Fludioxonil and 2.5% Difenoconazole EC). In the field, foliar spray of folicur, knowin and protaf was found ineffective to control bakanae disease. Looking for resistant one, 46 BRRI released varieties, “Purbachi”- a Chinese variety and 40 landraces of BRRI germplasm were tested in vitro against the pathogen. All these varieties and landraces were susceptible to the test pathogen in various degrees. Among the r...
Dhaka University Journal of Biological Sciences, 2014
DOI: http://dx.doi.org/10.3329/dujbs.v23i1.19833 Dhaka Univ. J. Biol. Sci. 23(1): 97-99, 2014
Dhaka University Journal of Biological Sciences, 2014
The incidence of anthracnose disease was observed lowest in December (10.87%) and highest in July... more The incidence of anthracnose disease was observed lowest in December (10.87%) and highest in July (80.50%) at the three selected betel vine fields of Shalon village in Moulvibazar district. The young stems were found more prone to attack than the old ones. Pathogenicity test showed Colletotrichum dematium and C. gloeosporioides produced characteristic disease symptoms on betel stems. Among them C. dematium is a new document as a pathogen of anthracnose of betel vine. Out of five fungicides, complete inhibition of both the pathogens in vitro occurred with corozole EC (25% propiconazole), folicur EC (25% tebuconazole) and trooper WP (75% tricyclazole). Among the four antagonistic fungi, Penicillium sp. showed more antagonistic potentiality against the pathogens. DOI: http://dx.doi.org/10.3329/dujbs.v23i2.20092 Dhaka Univ. J. Biol. Sci. 23(2): 127-133, 2014
Bangladesh Journal of Botany, 2014
Several varietal screening methods were tested along with dipping dry seeds into millipore membra... more Several varietal screening methods were tested along with dipping dry seeds into millipore membrane filtrate of spore suspension of the pathogen and gibberellic acid (GA3) in different concentrations. It was found that GA3 can be used to screen out susceptible rice varieties against bakanae disease. The method which requires the use of GA3 is easier than the other methods for mass screening as it does not require maintaining living culture of the pathogen. The protocol of inoculum free varietal screening method for bakanae disease of rice was developed to find out resistant varieties from the huge collections of germplasm bank. DOI: http://dx.doi.org/10.3329/bjb.v42i2.18036 Bangladesh J. Bot. 42(2): 315-320, 2013 (December)
Bangladesh Journal of Botany, 2014
In an average, 2106 fungal colony forming units were settled within ten minutes on one square met... more In an average, 2106 fungal colony forming units were settled within ten minutes on one square meter area at noon from the air of Dhaka University campus during February 2011 to January 2012. Among the identified fungi, Aspergillus was one of the most dominating genus in all the stations over the study period. The second was Penicillium followed by Cladopsorium, Curvularia, Alternaria, Fusarium, Trichoderma, Pestalotia, Rhizopus and Colletotrichum. In the dry winter (December - February), Alternaria, Cladosporium, Curvularia and Rhizopus showed its peak. Hot humid summer (April) is the most favourable season for the occurrence of Colletotrichum. Similarity has been found in fungal biodiversity in the indoor and outdoor air. However, higher number of colony was recorded from indoor (57.23%) than that of outdoor air. DOI: http://dx.doi.org/10.3329/bjb.v42i2.18029 Bangladesh J. Bot. 42(2): 273-278, 2013 (December)
Bangladesh Journal of Botany, 2008
Fifty five angiospermic plants were selected for evaluating the effect of their aqueous extracts ... more Fifty five angiospermic plants were selected for evaluating the effect of their aqueous extracts on the in vitro vegetative growth of Fusarium moniliforme Sheldon. Extracts of 17 plants showed varied degrees of inhibitory effects on the test pathogen. For instance the leaf extract of Lawsonia inermis showed maximum inhibition (60.65 %) followed by roots of Asparagus racemosus (50.59 %). The possibility of using these plant extracts in seed treatment to control bakanae disease of rice is suggested. Antifungal property of leaves of Andrographis paniculata and Lagerstroemia speciosa against bakanae disease is reported here for the first time. Key words: In vitro, Vegetative growth, Bakanae, Fusarium moniliforme, Plant extracts doi:10.3329/bjb.v37i1.1569 Bangladesh J. Bot. 37(1): 85-88, 2008 (June)
Bangladesh Journal of Botany, 2015
Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigat... more Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested in opposition to six pathogenic fungi viz., Colletotrichum sp., Curvularia lunata, Fusarium moniliforme, F. oxysporum, F. semitectum and Phomopsis sp. isolated from different leaf spots and fruit rots of brinjal. Out of seven soil fungi, Trichoderma harzianum was found most effective to control the growth of all the test fungi in the study of colony interactions and effects of volatile and non-volatile metabolites. This fungus may be exploited commercially to biocontrol the diseases. DOI: http://dx.doi.org/10.3329/bjb.v43i2.21675 Bangladesh J. Bot. 43(2): 213-217, 2014 (September)
Damping off of seedlings of Cabbage, Cauliflower and Kohlrabi is very common in the nursery beds ... more Damping off of seedlings of Cabbage, Cauliflower and Kohlrabi is very common in the nursery beds of the Dhaka city. ...
Density of entomopathogenic fungi spores in the larval habitat water of Culex quinquefasciatus wa... more Density of entomopathogenic fungi spores in the larval habitat water of Culex quinquefasciatus was conducted at the Entomology laboratory and Plant Pathology laboratory, Jagannath University, Dhaka, Bangladesh. The larvae and pupae of different mosquito species were counted; water was preserved for fungal culture. Potato Dextrose Agar (PDA) medium was used for culture and different levels of dilution were performed for culture of fungus spores. These stagnant dirty drain water samples have been containing a few (10) Aedes larvae; coexisting with a huge Culex at the same breeding ground. A total of 4138 mosquito larvae and pupa were recorded in the collected habitat water where 3928 larvae and 200 pupa of Culex were observed with different larval densities among collection points. Out of eighteen (18) fungal isolates under 10 genera (Absidia sp., Aspergillus sp., Cladosporium sp., Curvularia sp., Fusarium sp., Geotrichum sp., Nigrospora sp., Penicillium sp., Rhizopus sp. and Sclerotium sp.) were identified, seven (7) of them have been reported as entomopathogenic by scientists; to date. Eight (8) isolates were belonging to the genus Aspergillus. Relationship of larval density of Culex were very weak to total spores of all fungi (r =-0.154) as well as with the number of individual isolates of fungus spores. Culture of entomopathogenic fungi in laboratory condition, and use of them as a biological control agent for the mosquitoes could not be recommended.
Silver nanoparticles (Ag-NPs) by mixing silver nitrate (AgNO3) with cell-free filtrate (CFF) of t... more Silver nanoparticles (Ag-NPs) by mixing silver nitrate (AgNO3) with cell-free filtrate (CFF) of the two fungal isolates viz., Fusarium 4F1 and Trichoderma TrS were synthesized. pH, substrate concentration and incubation period for the production of better quality and quantity of Ag-NPs was optimized. The Ag-NPs by UV-vis spectroscopy were characterized. Between the two fungal isolates, pH levels, AgNO3 concentrations and incubation periods studied, the highest number of spherical shaped, monodispersed and stable Ag-NPs were recorded from Fusarium 4F1 at pH 9, 2 mM AgNO3 and 72 hrs of incubation.
IMC J Med Sci, 2019
Background and objectives: Candida auris is an emerging multidrug-resistant fungal pathogen that ... more Background and objectives: Candida auris is an emerging multidrug-resistant fungal pathogen that has been associated with nosocomial infections with a high mortality. The organism has been reported from several countries of the world except Bangladesh. The present study describes the presence of C. auris in clinical samples obtained from a large hospital of Dhaka city, Bangladesh.
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Papers by Prof. Dr. Kazi Shakhawath Hossain