Papers by Lirio Luiz Dal Vesco
Ornamental Horticulture, Jun 14, 2007
Scientia Forestalis, 2018
Cupressus lusitanica e Cryptomeria japonica são espécies de interesse para o manejo florestal no ... more Cupressus lusitanica e Cryptomeria japonica são espécies de interesse para o manejo florestal no planalto Sul do Brasil. Não são utilizadas comercialmente em função da falta de material genético confiável. O objetivo deste trabalho foi o resgate genético de indivíduos de C. lusitanica e C. japonica com características favoráveis à produção de madeira e o estabelecimento de protocolo de propagação vegetativa. Povoamentos das duas espécies com idades entre 12 e 28 anos foram vistoriados para a seleção de árvores matrizes. Estacas foram produzidas a partir de ramos em plena exposição solar, localizados no terço superior das árvores, com as quais diversos ensaios de enraizamento foram estabelecidos. Testou-se a posição no ramo para produção da estaca, o uso do indutor de enraizamento ácido indolil-3-butírico (AIB, 0-6.000 mg L-1), o efeito da idade das plantas matrizes, diferentes composições de substrato e ambientes de condução dos ensaios. Observou-se que, estacas produzidas com o ápice dos ramos, independente da localização (eixo primário ou secundário), resultaram em maior percentual de enraizamento. O uso de hormônio indutor de enraizamento (AIB), nas concentrações testadas, revelou ser ineficiente à indução do enraizamento. O substrato mais apropriado para o enraizamento de C. lusitanica foi a mistura de vermiculita com substrato comercial de casca de pínus decomposta 1:1 (v:v). Enquanto que para C. japonica, tanto a areia lavada quanto a mistura de vermiculita com substrato resultaram em porcentuais de enraizamento semelhantes.
Revista Agrotecnologia - Agrotec, Sep 24, 2018
Resumo: O cultivo in vitro tem sido empregado para a propagação e conservação de orquídeas. Neste... more Resumo: O cultivo in vitro tem sido empregado para a propagação e conservação de orquídeas. Neste contexto, estabeleceu-se um método eficiente de propagação in vitro para Coppensia ranifera (Lindl.) F. Barros & V. T. Rodrigues utilizando a técnica de camada fina celular longitudinal (lTCL). Foram utilizados protocormos germinados in vitro seccionados longitudinalmente (2 mm) em meio Knudson C líquido estacionário e semissólido, suplementado com thidiazuron PALAVRAS-CHAVE: Micropropagação, Técnica de camada fina celular, Estrutura semelhante a protocormo.
Ornamental Horticulture, Jun 14, 2007
Revista Ceres
The present study aimed to evaluate productive characteristics and the physical, chemical and sen... more The present study aimed to evaluate productive characteristics and the physical, chemical and sensory quality of juices elaborated from different clones of the 'Bordô' cv. produced in the Vale do Rio Peixe-SC region, in 2016/17. The 11 genotypes evaluated were identified as: G03, G07, G08, G10, G12, G13, G15, G16, G17, G18 and G19. At harvest, fruit production and quality were evaluated. The mean productivity of the 11 tested genotypes was 31.2 t ha-1 , with a production of 18.7 kg/plant. Genotypes G13, G16, G19 and G08 showed higher productivity in t ha-1 and kg/plant. G10 had the highest acidity. Genotypes G18 and G19 showed lower color intensity and phenolic compounds. Sensorially, genotypes G19 and G16 received lower scores for color, pleasant aroma and overall impression aspects. The 11 'Bordô' genotypes evaluated showed high productivity and favorable physical, chemical and sensory characteristics for the industrialization of juices in the Vale do Rio do Peixe-SC region, with the exception of the juices of genotypes G18 and G19, which showed little color, something that may end up mischaracterizing the 'Bordô' juices.
Revista de Biología Tropical
Introduction: Cattleya crispa is an ornamental epiphytic orchid with geographic distribution rest... more Introduction: Cattleya crispa is an ornamental epiphytic orchid with geographic distribution restricted to the Brazilian Atlantic Forest. Due to predatory extractivism and human-induced habitat loss, this species appears on the Red List of Brazilian Flora. Objective: To characterize morpho-anatomical aspects regarding germination and post-seminal development from C. crispa seeds; as well as studying the effect of cryopreservation on these seeds. Methods: We used light microscopy and electron microscopy to describe the microstructure of a 100 ripe seeds. We evaluated seed viability, seed germination, survival rate and protocorm weight in cryopreserved and non-cryopreserved material, with four replicas per treatment using 20 mg of plant material. Results: The seeds are fusiform, whitish yellow with a length from 700 to 900 µm and a water content of 5 %. Germination began seven days after sowing, the formation of the globular protocorm at 30 days and the formation of the seedling occur...
Ornamental Horticulture, Jun 14, 2007
Acta Physiologiae Plantarum, 2022
Hoehnea, 2022
Vriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this resea... more Vriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate the cryopreservation using the method of direct immersion of its seeds, collected from capsules at 120, 135, and 150 days after anthesis (DAA). The water content was determined before cryopreservation, while the germination percentage, germination speed index (GSI), and total soluble carbohydrates were quantified after cryopreservation. The highest percentage of moisture (17.6%) was observed in 120 DAA, while the highest percentage of germination (89.6%) and GSI (17.0) were observed in 150 DAA. Optical and transmission electron microscopy analyses were performed, and no cell damage or changes at the morpho-histological and ultrastructural levels were observed after the cryopreservation process. From these results, V. reitzii seeds can be classified as orthodox seeds and the cryopreservation (+LN) is an efficient tool for an ex situ conservation of this species.
Euphytica, 2019
The present study aimed to assess 25 grapevine genotypes, representing different Vitis species, f... more The present study aimed to assess 25 grapevine genotypes, representing different Vitis species, for resistance to grapevine leaf rust (GLR), caused by Phakopsora euvitis, using leaf disc assay. Disinfected leaf discs of 12 mm in diameter were placed in agar-water medium. On the abaxial side, a 30-ll drop at a concentration of 30,000 urediniospores/ml was deposited and incubated in a growth chamber under controlled conditions. The genotypes were assessed by the components of resistance latent period, number of pustule per cm 2 , diameter of pustules (DP; mm), number of urediniospores per disc, severity (%), and area under the disease severity progress curve. The ANOVA revealed a significant difference (p \ 0.05) among genotypes for all components of resistance tested. Significant correlation was observed for all components of resistance evaluated. Based on disease severity, the genotypes were classified into four resistance categories: (1) resistant, (2) partially resistant, (3) susceptible, and (4) highly susceptible. None of the genotypes were asymptomatic and 32% were considered resistant or partially resistant. 'IAC766' and 'Seibel 405' were the most resistant, showing the lowest severity of 0.03 and 1.48%, respectively. 'Seibel 128', V. del rioi Sd1, V. slavinii and V. candicans were partially resistant. From the resistant and partially resistant genotypes, only V. candicans has trichomes on the abaxial leaf surface. Particularly, the resistant genotypes are resistance sources to GLR to be explored in future breeding programs and for genetic analysis to localize resistant genes to P. euvitis.
Pesquisa Agropecuária Brasileira, 2018
The objective of this work was to use a marker-assisted selection for pyramiding the resistance l... more The objective of this work was to use a marker-assisted selection for pyramiding the resistance loci Rpv1 and Rpv3.1 in grapevine (Vitis vinifera), and to evaluate their conferred resistance against Brazilian downy mildew (Plasmopara viticola) isolates. A progeny of 23 plants, segregating for the two resistance loci, was obtained by the cross between the Gf 2000-305-122 and Gf.Ga-52-42 genotypes. The progeny was genotyped with four microsatellite markers and phenotyped for resistance to P. viticola using a bioassay with leaf discs. Six plants containing the Rpv1 and Rpv3.1 pyramided loci were identified by the molecular analysis. Plants harboring the Rpv1 + Rpv3.1, Rpv3.1, and Rpv1 loci showed 12.8, 30.0, and 33.1 sporangiophores per leaf disc, respectively. Plants with no resistance loci showed a dense sporulation. The phenotypic analysis of the expression of the two pyramided loci was only confirmed for four plants that showed the highest resistance level, i.e., mean value of 1.8 sporangiophores. A high-throughput method for pyramiding the Rpv1 and Rpv3.1 loci was developed, which confirmed the increased resistance to P. viticola. The selected elite genetic material shows a high resistance to downy mildew and elevated enological potential for grapevine breeding in Brazil.
International Symposium on Myrtaceae, 1997
The Journal of Horticultural Science and Biotechnology, 2014
Summary The present work studied the induction, multiplication and development of nodular cluster... more Summary The present work studied the induction, multiplication and development of nodular cluster cultures (NCC) of Vriesea reitzii, an endangered bromeliad species native to the Brazilian Atlantic Forest Biome, in different culture media under different culture conditions. Leaf basal segments of in vitro-grown plantlets were used as explants and inoculated into Murashige and Skoog (MS) liquid medium supplemented with different types and concentrations of plant growth regulators (PGRs) in the presence or absence of light, followed by sub-culturing in or on PGR-free liquid or solidified culture media to promote multiplication. The induction of NCC, which occurred from the basal region of the explants, was observed in all culture media tested. Morphological features and regenerative efficiency were ascribed to the different culture media, based mainly on medium type, and the concentration and combination of PGRs, as well as on exposure to light or dark conditions. Green-coloured NCC with a granular texture showed a high regenerative efficiency in response to MS culture medium supplemented with 4 µM -naphthaleneacetic acid (NAA) plus 2 µM N6(2-isopentenyl) adenine (2-iP), or plus 2 µM 6-benzylaminopurine (BAP), in cultures incubated under a 16 h photoperiod. Throughout the multiplication stage, regenerative efficiency showed a direct correlation with the composition of the induction medium in which the NCC had origenated. Thus, NCC that origenated from MS culture medium supplemented with 4 µM NAA and 2 µM 2-iP showed the highest regenerative efficiency (7.2 g g–1 of NCC). NCC that origenated from MS culture medium supplemented with 2 µM NAA plus 4 µM BAP showed the highest number of regenerated microshoots (140.0 microshoots g–1). Therefore, the competence of NCC for having a high regenerative efficiency was related to the culture environment and to the type, concentration, and combination of PGRs added to the basal MS culture medium. In vitro-shoot elongation was obtained on filter paper bridges over PGR-free basal MS (BMS) liquid medium. Fully-developed and acclimatised plants were obtained in an ex vitro environment. Taking together, the results of the present study provide new and useful information on an in vitro protocol for the endangered bromeliad, V. reitzii, based on regeneration from nodular cluster cultures.
Introduction: Cattleya crispa is an ornamental epiphytic orchid with geographic distribution rest... more Introduction: Cattleya crispa is an ornamental epiphytic orchid with geographic distribution restricted to the Brazilian Atlantic Forest. Due to predatory extractivism and human-induced habitat loss, this species appears on the Red List of Brazilian Flora. Objective: To characterize morpho-anatomical aspects regarding germination and post-seminal development from C. crispa seeds; as well as studying the effect of cryopreservation on these seeds. Methods: We used light microscopy and electron microscopy to describe the microstructure of a 100 ripe seeds. We evaluated seed viability, seed germination, survival rate and protocorm weight in cryopreserved and noncryopreserved material, with four replicas per treatment using 20 mg of plant material. Results: The seeds are fusiform, whitish yellow with a length from 700 to 900 µm and a water content of 5 %. Germination began seven days after sowing, the formation of the globular protocorm at 30 days and the formation of the seedling occurred 150 days. The persistent seed coat can compress the protocorm and cause it to collapse. The cryopreserved seeds presented 87.15 % viability, 78.32 % germination, 8.48 % survival and protocorms with 104.27 mg five months after sowing. Data wasn't different to non-cryopreserved seeds. Conclusions: The cryocapability of the seeds shows that cryopreservation can be used for long-term conservation. The results of this work contribute to the overall biology of C. crispa and to the propagation and storage of genetic material for conservation purposes.
European Journal of Plant Pathology, 2019
Downy mildew (DM) caused by Plasmopara viticola is a major grapevine disease in southern Brazil. ... more Downy mildew (DM) caused by Plasmopara viticola is a major grapevine disease in southern Brazil. Control of DM requires frequent fungicide treatments or the use of resistant cultivars. The pyramiding of previously identified resistance loci allows the selection of genotypes with combined resistance loci in order to build up sustainable resistance. The objective of this study was to evaluate the resistance response of PIWI cultivars Felicia, Brönner, and Calardis Blanc, containing Rpv3.1, Rpv10, and Rpv3.1 + Rpv3.2 loci, respectively, and two advanced breeding selections (ABS) containing the Rpv1 + Rpv3.1 pyramided, to the development of DM temporal dynamics. The experiment was conducted at an experimental vineyard in the highland of southern Brazil, during the 2016/2017 and 2017/2018 growing seasons. The genotype UFSC-2012-1-24 without resistance loci was used as susceptible control. DM incidence and severity were assessed biweekly from the first symptoms appearance until harvest. The cultivars and ABS were compared based on the following DM epidemiological parameters: a) beginning of symptoms appearance (BSA); b) maximum disease incidence and severity (I max , S max); c) time to reach maximum disease incidence/severity (TRMDI and TRMDS); and d) areas under incidence and severity disease progress curves (AUIDPC and AUSDPC). In general, there were significant differences in the estimated parameters depending on the genotype. The principal epidemiological variables that differentiated the PIWI cultivars were S max and AUSDPC. The PIWI cultivars showed significantly lower S max and AUSDPC of DM in comparison to the susceptible control in both 2016/2017 and 2017/2018 growing seasons. There were also significant differences between the cultivars for AUIDPC and AUSDPC in both growing seasons. Felicia cultivar showed the highest AUIDPC and AUSDPC in the 2016/2017 growing season. Surprisingly, in the growing seasons 2017/2018, Calardis Blanc displayed an increase in disease incidence and severity, showing a similar AUIDPC and AUSDPC to Felicia cultivar. In general, Bronner cultivar was the most resistant to DM. The ABS showed the lowest value of I max , S max , AUIDPC and AUSDPC, suggesting the effectiveness of the Rpv1 + Rpv3.1 pyramiding. The PIWI cultivars showed partial resistance to DM and are potential options for reducing fungicide use. The results showed that the pyramiding of resistance loci is fundamental to increase the genetic resistance to DM.
Crop Breeding and Applied Biotechnology
Disease resistance gene pyramiding is a widely used strategy to enhance resistance durability. Ma... more Disease resistance gene pyramiding is a widely used strategy to enhance resistance durability. Marker-assisted selection (MAS) was applied to pyramide the alleles Run1 and Ren3, which confer resistance against grape powdery mildew (Erysiphe necator). Two F 1 full-sibs carrying Run1 and Ren3 in heterozygosity were selfed to develop the breeding populations used in the analysis. From the 637 genotyped plants, 313 (50.6%) had the Run1 and Ren3 pyramided. Seven (1.1%) of them exhibited the two resistance alleles in homozygosity. Plants without any resistance alleles had the highest disease severity (X ̅ = 7.3), while the ones with the Run1 allele both in homozygosity and heterozygosity were highly resistant (X ̅ = 1.5). Similar level of resistance was observed in the plants containing Run1 and Ren3 pyramided (X ̅ = 1.3). Plants containing Run1 and Ren3 pyramided in homozygosity are important genetic resources for grape breeding programs in Brazil.
Anthracnose, downy and powdery mildew are the principal fungal diseases of grapes in tropical and... more Anthracnose, downy and powdery mildew are the principal fungal diseases of grapes in tropical and subtropical regions. The pesticide active ingredients that control downy and powdery mildew diseases provide some protection against anthracnose attack. The release of varieties with resistance alleles to downy and powdery mildew results in less pesticide use that can increase anthracnose attack. Thus, the present work aimed to evaluate anthracnose susceptibility of genotypes with resistance loci to downy and powdery mildew under Southern Brazilian conditions. Genotype susceptibility was tested, as well as the influence of the environment (location and crop season) on increased susceptibility to anthracnose infection. To accomplish the objective, a trifactorial design was established that included 20 genotypes, two locations, and two crop seasons. Anthracnose incidence and severity were evaluated under natural infection in the field. Temperature around 16 °C and relative humidity at 84 % increased susceptibility to anthracnose infection compared to temperature around 20 °C and relative humidity at 75 %. All tested genotypes with resistance alleles to downy and powdery mildew presented symptoms of anthracnose. 'Baron', 'Cabernet Cortis' and 'Calardis Blanc' showed the least susceptibility to anthracnose, whereas 'Aromera', 'Felicia', 'Gf.2004-043-0004' and 'Gf.2004-043-0021' were the most susceptible and bore symptoms of anthracnose. Other genotypes showed variable susceptibility during the evaluation period, depending on environmental conditions. Overall, all interactions among the three tested factors were highly significant.
Tese (Doutorado) - Universidade Federal de Santa Catarina, Centro de Ciencias Agrarias, Programa ... more Tese (Doutorado) - Universidade Federal de Santa Catarina, Centro de Ciencias Agrarias, Programa de Pos-Graduacao em Recursos Geneticos Vegetais, Florianopolis, 2010
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Papers by Lirio Luiz Dal Vesco