Stochastic mRNA Synthesis in Mammalian Cells
Figure 3
Statistical Analysis of Per-Cell mRNA Population Distributions
(A) Histograms showing the distribution of mRNA molecules per cell for three doxycycline concentrations for both cell lines E-YFP-M1-1x (left) and E-YFP-M1-7x (right).
(B) Graphs showing the population mean (top) and noise (defined as the standard deviation divided by the mean [bottom]) as a function of doxycycline concentration. Statistics were taken from the mRNA counts used in (A), and error bars were obtained by bootstrapping.
(C) Activation rate (λ/δ) and average number of mRNA molecules produced per burst (μ/γ) for 1x-tetO (blue) and 7x-tetO (red) obtained from fitting the mRNA count data to the model of gene activation and inactivation by the maximum-likelihood method. Error bars reflect 95% confidence intervals.