Yeast Silent Mating Type Loci Form Heterochromatic Clusters through Silencer Protein-Dependent Long-Range Interactions
Figure 8
Silencing and HM interactions are distinct processes.
RT-PCR analysis of a1 transcription in wild-type and mutant MATα strains analyzed by 3C. Error bar represents standard error of the mean from two independent experiments. Expression of ADH1 was used as an internal control and data was normalized to level of expression in sir3Δ mutants (set at 1). Inset: Crosslinking frequency of HML-HMR interaction is plotted versus the relative mRNA expression of a1 for all mutants analyzed to indicate that no clear correlation between silencing and HM interactions is detected.