Single Cell RNA-seq

The 10x Genomics Chromium is a microfluidics device capable of partitioning single cells into nanoliter emulsions for transcriptomic and genomic analyses. The Arts & Sciences Imaging Center offers full service processing of single cells into bar-coded cDNA libraries ready for sequencing on an Illumina platform. The service includes:

• Evaluation, quantification, and viability assessment of cells for input
• Single cell capture of desired target number of cells (500-20,000)
• Generation and amplification of single cell cDNAs
• Construction of Illumina sequencing-ready libraries of cDNA
• Quality assessment and quantification of libraries

Note that services do NOT include Illumina sequencing or bioinformatic analysis. Sample processing must be scheduled at least two weeks in advance. Please contact us for more information or to schedule a consultation about a project.
 

Spatial Transcriptomics

The 10x Genomics Visium and Visium HD platforms power the interrogation of transcription within the spatial context of intact tissues. Starting with sections from frozen, fixed frozen, or FFPE tissues, the entire transcriptome is probed or captured onto a 6.5 x 6.5mm lawn of 55 micron spots (Visium) or 2 micron squares (Visium HD) for spatial barcoding and library generation. The services include:

• H&E staining and imaging of tissue sections
• Capture of transcripts onto Visium slides using the CytAssist instrument
• Generation of Illumina sequencing-ready libraries from captured transcripts
• Quality assessment and quantification of libraries

As with single cell, services do NOT include Illumina sequencing or bioinformatic analysis. Please contact us for more information or to schedule a consultation about a project.

The A&S Imaging Center has partnered with the Markey Cancer Center Oncogenomics Shared Resource Facility to create the University of Kentucky Single Cell Researchers Network whose goal is to serve as a community resource and user forum for investigators across campus to learn and share experiences and expertise with single cell analysis methods.  To receive news and announcements from the UK scRN, join the SingleCell email listserv.