Papers by Ahmad Asnawi Mus
Pecah Beling (Strobilanthes crispus) is a shrub that is often used in traditional medicine. It c... more Pecah Beling (Strobilanthes crispus) is a shrub that is often used in traditional medicine. It contains various essential bioactives such as phenolics, flavonoids and terpenoids, that contributes to its biological activities such as anticancer, antidiabetic and anti-inflammatory properties. Due to the potential this plant possesses in various health applications, tissue culture techniques offer the best solution to further enhance S. crispus production as raw material for herbal product preparation. The objective of this research is to study the effect of cytokinins alone or in combination on shoot induction from nodal explants of S. crispus. Nodal parts of in vitro cultures of S. crispus were used as explants and cultured in MS (Murashige and Skoog) medium containing BAP (Benzylaminopurine), KN (Kinetin) and TDZ (Thidiazuron) at concentrations between 0.5mg/L to 4mg/L. Treatment of nodal explant with single cytokinin were able to induce shoots, with 59.8% to 100% induction percentage and 1.1mm to 5.2mm mean shoot length. However, mean number of shoots induced are low, ranging only from 1 to 3.1 shoots produced per explant. Further treatment using combinations of BAP (0.5mg/L) or Kn (0.5mg/L) with increasing TDZ concentration (0.05mg/L to 0.4mg/L) were able to induce multiple shoots from nodal explant of S. crispus, where the best treatment was Kn 0.5mg/L + TDZ 0.2mg/L, where it was able to produce 9.4 ± 2.75 shoots per explant with 4.28 ± 1.55mm shoot length and 79.8% induction percentage. As a conclusion, the high frequency of shoots from S. crispus nodal explant was better achieved in cytokinin-cytokinin combination treatment compared to single cytokinin treatment.
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Papers by Ahmad Asnawi Mus