Papers by Joanne Katherine T . Manlusoc
Polymers, 2019
With the advent of exploration in finding new sources for treating different diseases, one possib... more With the advent of exploration in finding new sources for treating different diseases, one possible natural source is from marine algae. Having an array of potential benefits, researchers are interested in the components which comprise one of these activities. This can lead to the isolation of active compounds with biological activities, such as antioxidation of free radicals, anti-inflammation, antiproliferation of cancer cells, and anticoagulant to name a few. One of the compounds that are isolated from marine algae are sulfated polysaccharides (SPs). SPs are complex heterogenous natural polymers with an abundance found in different species of marine algae. Marine algae are known to be one of the most important sources of SPs, and depending on the species, its chemical structure varies. This variety has important physical and chemical components and functions which has gained the attention of researchers as this contributes to the many facets of its pharmacologic activity. In this...
Pharmaceutical Sciences and Research
Eleusine indica Linn. Gaerth or Indian goose grass has been widely used as an alternative natural... more Eleusine indica Linn. Gaerth or Indian goose grass has been widely used as an alternative natural anti-hypertensive agent. However, the rationale behind this mechanism is still unknown. The purpose of this study is to confirm the presence of functional groups that may be responsible for its ACE inhibitory activity and its possible synergism. Each solvent-portioned fraction was analyzed by FTIR, individual ACE inhibitory activity, and combination of fractions. FTIR results of solventpartitioned fractions revealed that O-H and C-H stretches were present in all fractions. In addition to this, CO and C=O groups were also present in all fractions except in ethyl acetate. S=O groups were also present in chloroform, ethyl acetate, and n-butanol fractions. Other function groups such as NO and C=C groups were present in ethyl acetate and n-butanol fractions, respectively. The highest % inhibition obtained among the individual fraction is n-butanol at 96.031 % ± 0.004 at 500 ug/ml. The lowest % inhibition obtained among the individual fraction is chloroform at 32.544 % ± 0.011 at 500 ug/ml. The highest % inhibition among the combined fractions is ethyl acetate:butanol at 95.727% ± 20.847, while the lowest % inhibition in combination is hexane:chloroform at 89.626 % ± 19.577. The fraction with the lowest IC 50 is hexane at 15.357, while the highest IC 50 is chloroform at 168.207 ug/ml. Functional group present may be associated with the antihypertensive activity of the E. indica extracts. The more polar solvent-partitioned fractions of E. indica has a higher ACE inhibitory activity compared to its less polar solventpartitioned fractions. The higher ACE inhibitory activity may be associated with the secondary metabolites present in the more polar fractions. There is also possible synergism present in the combined solvent-partitioned fractions as their % inhibition is higher than their individual effects.
Previous studies have shown that crude ethanolic extract of Tinospora rumphii Boerl exhibit pedic... more Previous studies have shown that crude ethanolic extract of Tinospora rumphii Boerl exhibit pediculocidal activity (Lagarejos et al., 2006). This study involves the formulation of a pediculicidal shampoo from the Makabuhay ethanolic extract using compatible excipients and the determination of its activity using Permethrin (Kwell) as positive control.
Stems of the plant Tinospora rumphii Boerl was percolated with 80% ethanol and was formulated together with a base to determine its pediculocidal effect. The stability of the crude ethanolic extract with three (3) shampoo formulations was determined by organoleptic, pH, and density determination. Patch and scratch test on test animal was also performed to determine its skin sensitivity and reaction in animals. No apparent irritation was noted after 72 hours of application. In-vitro study was also conducted to determine its lice killing activity. Preliminary clinical studies were also employed to five (5) respondents to test for the possibility of hypersensitivity and to further test its effectivity on human subjects.
Single-Factor ANOVA showed a significant difference between the mean killing time of the adult lice using the Makabuhay shampoo, Permethrin and the shampoo base (p<0.001). Human patch test result in thirty (30) subjects showed no significant irritation with the Makabuhay shampoo. ANOVA for Repeated Measures showed no significant interaction effect between the treatment used and the duration it was applied – amount of adult lice (p = 0.077), nits (p=0.580) and pruritus (0.519). Also, there was no significant difference on the number of adult lice (p=0.233) and nits (0.580) killed but a significant difference in pruritus (p<0.001) in regards to the treatment. However, in regards to the duration of treatment, there was no significant difference on the decrease of the number of adult lice (p<0.001) and pruritus (p=0.071) but a significant difference on the nits killed (0=0.02). Therefore, the shampoo formulated with the crude ethanolic extract from Tinospora rumphii has equal efficacy as that of the commercially available positive control (Permethrin).
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Papers by Joanne Katherine T . Manlusoc
Stems of the plant Tinospora rumphii Boerl was percolated with 80% ethanol and was formulated together with a base to determine its pediculocidal effect. The stability of the crude ethanolic extract with three (3) shampoo formulations was determined by organoleptic, pH, and density determination. Patch and scratch test on test animal was also performed to determine its skin sensitivity and reaction in animals. No apparent irritation was noted after 72 hours of application. In-vitro study was also conducted to determine its lice killing activity. Preliminary clinical studies were also employed to five (5) respondents to test for the possibility of hypersensitivity and to further test its effectivity on human subjects.
Single-Factor ANOVA showed a significant difference between the mean killing time of the adult lice using the Makabuhay shampoo, Permethrin and the shampoo base (p<0.001). Human patch test result in thirty (30) subjects showed no significant irritation with the Makabuhay shampoo. ANOVA for Repeated Measures showed no significant interaction effect between the treatment used and the duration it was applied – amount of adult lice (p = 0.077), nits (p=0.580) and pruritus (0.519). Also, there was no significant difference on the number of adult lice (p=0.233) and nits (0.580) killed but a significant difference in pruritus (p<0.001) in regards to the treatment. However, in regards to the duration of treatment, there was no significant difference on the decrease of the number of adult lice (p<0.001) and pruritus (p=0.071) but a significant difference on the nits killed (0=0.02). Therefore, the shampoo formulated with the crude ethanolic extract from Tinospora rumphii has equal efficacy as that of the commercially available positive control (Permethrin).
Stems of the plant Tinospora rumphii Boerl was percolated with 80% ethanol and was formulated together with a base to determine its pediculocidal effect. The stability of the crude ethanolic extract with three (3) shampoo formulations was determined by organoleptic, pH, and density determination. Patch and scratch test on test animal was also performed to determine its skin sensitivity and reaction in animals. No apparent irritation was noted after 72 hours of application. In-vitro study was also conducted to determine its lice killing activity. Preliminary clinical studies were also employed to five (5) respondents to test for the possibility of hypersensitivity and to further test its effectivity on human subjects.
Single-Factor ANOVA showed a significant difference between the mean killing time of the adult lice using the Makabuhay shampoo, Permethrin and the shampoo base (p<0.001). Human patch test result in thirty (30) subjects showed no significant irritation with the Makabuhay shampoo. ANOVA for Repeated Measures showed no significant interaction effect between the treatment used and the duration it was applied – amount of adult lice (p = 0.077), nits (p=0.580) and pruritus (0.519). Also, there was no significant difference on the number of adult lice (p=0.233) and nits (0.580) killed but a significant difference in pruritus (p<0.001) in regards to the treatment. However, in regards to the duration of treatment, there was no significant difference on the decrease of the number of adult lice (p<0.001) and pruritus (p=0.071) but a significant difference on the nits killed (0=0.02). Therefore, the shampoo formulated with the crude ethanolic extract from Tinospora rumphii has equal efficacy as that of the commercially available positive control (Permethrin).