Spinning Disk Confocal with Super-Resolution Capabilities:

This system offers a unique combination of high-speed imaging and exceptional resolution without the need for specific sample preparation processes. Unlike standard confocal, which scans a single point at a time, the spinning disk confocal scans hundreds of points simultaneously. The addition of super-resolution imaging allows researchers to surpass the diffraction limit, achieving resolution down to 120 nm. What can be imaged in confocal mode can be further visualized in super-resolution, providing a seamless transition between imaging modalities.

Advantages of the spinning disk system include:

• Significantly faster imaging rates than standard confocal

• Superior temporal resolution with high-speed imaging with the ability to resolve biological processes not feasible with standard confocal.

• Improved Z-stack resolution for thick specimens, embryos, and organoids.

• Advanced live cell imaging capability including multiple particle tracking studies.

• Significantly reduced phototoxicity due to reduced light exposure

• Reduced photobleaching and improved imaging stability

• Ideal for tracking fast dynamics (ms regime) and extended timelapse (>24 hours)

Super resolution advantages:

• Resolution of 120 nm (XY)

• Realtime super resolution as the image is being collected

• Compatible with standard samples and fluorophores (no need for special dyes or sample conditions)

• Live Cell super resolution allowing fast dynamics to be captured at 100 nm resolution (no need to fix samples)

Hamamatsu ORCA-Quest 2 qCMOS camera:

• Ultra-low readout noise performance

• Suppress crosstalk and high QE

• High-speed readout