Intro Chem 31 Plant Pigments

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Plants around us possess wonderful and vibrant colors.

From the green colors of the leaves to the wonderful complex colors of the flowers. These colors come from a surprisingly small number of plant pigments. The three main plant pigments are chlorophyll, anthocyanins, and carotinoids. Each of these produces their own colors. Green for chlorophyll, red for anthocyanins, and orange for carotinoids.(odec.ca) Spinacia oleracea , commonly known as spinach, is the leafy plant that is used in this experiment.(wikipedia.org) The only pigments that are on spinach are chlorophyll and carotenes.(www.umaine.edu) It is possible to separate these pigments collected in the extract from one another by the use of paper chromatography. In this process, the extract is placed on a filter paper, and then the paper is placed in an organic solvent. By capillary action, the organic solvent and the extract move up on the paper. Some pigments have higher affinity for the solvent than in other, therefore they move at a different rate. This experiment familiarizes us on the different pigments found on plants. Knowing and studying the different pigments in plants, and separating them are a fun thing to do. It helps the students to be an expert in separating pigments by the use of chromatography. This experiment can help in students future experiments. It is also very useful in pharmaceutical industry; learning to extract a herbal plant carefully can have a more positive effect on the medicine.(motherearthliving.com) In this experiment, the students must be able to separate and identify the pigments present and to be able to compute for the rate of flow. http://www.odec.ca/projects/2003/thoga3n/pu blic_html/plantpig.html http://www.umaine.edu/nsfgk12/images/PDFs/PlantPigments.pdf http://www.motherearthliving.com/health-andwellness/herbal-extracts.aspx http://en.wikipedia.org/wiki/Spinach

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