Light Intensity and Transpiration

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Investigation of light intensity and its effect on the rate of transpiration

Introduction Transpiration is the loss of water vapour by evaporation from leaves and the stem. The water is lost through openings called stomata. Transpiration occurs because water molecules evaporate from cellulose walls of cells in the leaf which are saturated with water. This makes the air in the air spaces between mesophyll cells more or less saturated with water vapour. If the air outside the plant is less humid and the stomata are open, water vapour will diffuse out into the drier air outside. Therefore the rate of transpiration is affected by the water concentration gradient between the outside and the inside of the plant. Therefore the transpiration process is affected by a range of abiotic factors such as light, temperature, wind and humidity. In the experiment, the effect of different light intensities on the rate of transpiration is investigated. Therefore the other abiotic factors have to be controlled variables so that only the changing light intensity has an effect on the rate of transpiration as the independent variable. Light affects transpiration because the stomata tend to open in the light which is essential for loss of water vapour from the leaf. There is also an indirect effect of light as it contains infra-red rays which warm the leaf, thus raise its temperature. Therefore the rate of transpiration should increase as light intensity increases. Aim To determine the effect of different light intensities on the rate of transpiration in a leafy shoot of a lilac plant. Variables Independent Variable Variable Light intensity Why it needs to be changed In the experiment, it is investigated how light intensity affects the rate of transpiration in plants. The experiment needs to be carried out with a range of different light intensities to gain a reliable result that shows how the rate of transpiration is affected by different light intensities. How the variable is changed Light intensity is inversely proportional to the square distance from the source. Light intensity decreases when the distance between the light source and the plant increases. To ensure a range of different light intensities, the distance between the cold cathode (only light source) and the leafy shoot will be 0.5m, 1.0 m, 1.5m, 2m. In addition one set of 5 trials will be taken without light of the cold cathode to determine the rate of transpiration as a control to compare.

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