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    Polyketide Review

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    Digva Willyan
    Polyketides are secondary metabolites produced by microorganisms that exhibit diverse structures and functions, including many pharmacologically important compounds. They are synthesized by large enzyme complexes called polyketide synthases (PKS) that build up carbon chains from simple precursors. There are three main types of PKS - modular, iterative, and assembly line. Modular PKS contain modules that each add a unit and can modify it through reductive domains, allowing complex macrolides to be produced like erythromycin. Researchers are studying PKS to manipulate their activity through genetic engineering to produce novel pharmaceutical compounds.

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    Polyketide Review

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    Digva Willyan
    227 views11 pages
    Polyketides are secondary metabolites produced by microorganisms that exhibit diverse structures and functions, including many pharmacologically important compounds. They are synthesized by large enzyme complexes called polyketide synthases (PKS) that build up carbon chains from simple precursors. There are three main types of PKS - modular, iterative, and assembly line. Modular PKS contain modules that each add a unit and can modify it through reductive domains, allowing complex macrolides to be produced like erythromycin. Researchers are studying PKS to manipulate their activity through genetic engineering to produce novel pharmaceutical compounds.

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    Polyketides are secondary metabolites produced by microorganisms that exhibit diverse structures and functions, including many pharmacologically important compounds. They are synthesized by large enzyme complexes called polyketide synthases (PKS) that build up carbon chains from simple precursors. There are three main types of PKS - modular, iterative, and assembly line. Modular PKS contain modules that each add a unit and can modify it through reductive domains, allowing complex macrolides to be produced like erythromycin. Researchers are studying PKS to manipulate their activity through genetic engineering to produce novel pharmaceutical compounds.

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    227 views11 pages

    Polyketide Review

    Uploaded by

    Digva Willyan
    Polyketides are secondary metabolites produced by microorganisms that exhibit diverse structures and functions, including many pharmacologically important compounds. They are synthesized by large enzyme complexes called polyketide synthases (PKS) that build up carbon chains from simple precursors. There are three main types of PKS - modular, iterative, and assembly line. Modular PKS contain modules that each add a unit and can modify it through reductive domains, allowing complex macrolides to be produced like erythromycin. Researchers are studying PKS to manipulate their activity through genetic engineering to produce novel pharmaceutical compounds.

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    THE WORLD OF POLYKETIDES


    The growth in the field of polyketides has advanced at such a pace that no short review can hope to be comprehensive. Indeed the September 1997 issue of Chemical Reviews is devoted completely to discussion of a few representative polyketides and polypeptides.

    What are Polyketides?


    Microorganisms make a wealth of unusual metabolites that have a secondary role in the organism's ontogeny, such as self-defence, aggression, or even communication, as the need arises. Polyketides are such a group of secondary metabolites, exhibiting remarkable diversity both in terms of their structure and function. Polyketide natural products are known to possess a wealth of pharmacologically important activities, including antimicrobial, antifungal, antiparasitic, antitumor and agrochemical properties. These metabolites are ubiquitous in distribution and have been reported from organisms as diverse as bacteria, fungi, plants, insects, dinoflagellates, mollusks and sponges. The wide spectrum of acticvity of polyketides makes them economically, clinically and industrially the most sought after molecules. Many polyketide products are well-known compounds such as Erythromycin A, a broad spectrum macrolide antibiotic, the antihelmintic agent avermectin or the immunosuppresants FK506 and rapamycin. Oleandomycin, rifamycin, lovastatin, oxytetracycline and reserveratrol are a few more of the thousands of polyketides discovered so far. Polyketides are usually categorised on the basis of their chemical structures. Here are some examples of the myriad chemical structures a polyketide can take: Figure 1

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    Synthesis and Asssembly of polyketides


    Polyketides are synthesized by sequential reactions catalysed by a collection of enzyme activities called polyketide synthases (PKSs). These are large multienzyme protein complexes that contain a coordinated group of active sites. The biosynthesis occurs in a stepwise manner from simple 2-, 3-, 4-carbon building blocks such as acetyl-CoA, propionyl CoA, butyryl-CoA and their activated derivatives, malonyl-, methylmalonyl- and ethylmalonyl-CoA. Figure 2

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    The key chain-building step of polyketide biosyhnthesis is a decarboxylative condensation analogous to the chain elongation step of classical fatty acid biosynthesis which occurs in the following well-understood way: Figure 3

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    Unlike fatty acid biosynthesis, however, in which each successive chain elongation step is followed by a fixed sequence of ketoreduction, dehydration and enoyl reduction as shown above, the individual chain elongation intermediates of polyketide biosynthesis undergo all, some, or none of these functional group modifications, resulting in a striking level of chemical complexiyty in the products. Additional degrees of complexity arise from the use of different starter units and chain elongation units as well as the generation of new stereo-isomers.

    Types of Polyketide Synthases (PKSs)


    At least three architecturally different types of PKSs have been discovered in the microbial world.

    Type I systems consist of very large multifuctional poroteins which can be either processive ( for
    example the unique modular systems responsible for synthesis of macrolides like erythromycin, rapamycin, rifamycin etc.) or iterative (for example the lovastatin nonaketide synthase shown below). Iterative Type I synthases are analogous to vertebrate fatty acid synthases. These are typically involved in the biosynthesis of fungal polyketides such as 6-methylsalicylic acid and aflatoxin. These PKSs are large multidomain proteins carrying all the active sites required for polyketide biosynthesis. Figure 4

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    The iterative Type II systems consist of complexes of mono-functional proteins exemplified by the actinorhodin PKS from Streptomyces coelicolor. In these synthases, active sites are distributed among several smaller, typically monofunctional polypeptides. Type II synthases catalyse the formation of compounds that require aromatization and cyclization, but not extensive reduction or reduction/dehydration cycles. These PKSs are analogous to bacterial Fatty Acid Synthases and are involved in the biosynthesis of bacterial aromatic natural products such as actinorhodin, tetracenomycin and doxorubicin. The following cartoon depicts the hypothetical "dynamic" model of an aromatic polyketide synthase: Figure 5

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    Type III polyketide synthases are responsible for the synthesis of chalcones and stilbenes in
    plants and polyhydroxy phenols in bacteria. Chalcone synthase like proteins are comparatively small proteins with a single polypeptide chain and are involved in the biosynthesis of precursors for flavonoids. Unlike all other PKSs, these proteins do not have a phosphopantetheinyl (P-Pant) arm on which the growing polyketide chains are tethered. Figure 6

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    Modular polyketide synthases


    Modular PKSs constitute a unique class of Type I polyketide synthases. Each of these proteins consists of multiple active domains organised into modules. Each module is responsible for the construction of a carbon-carbon bond, via the decarboxylative condensation of a ketide extender unit with the growing polyketide chain, followed by a programmed reductive cycle. In addition there is a loading module in most PKSs for obtaining the starter unit at the front of module 1 and a thioesterase responsible for unloading the product at the end of the last module. The biosynthesis of 6-deoxyerythronolide B (6-dEB) has become the text-book example for modular polyketide synthases. In Saccaropolyspora erythrae, it is converted to the biologically active erythromycin by hydroxylation followed by addition of sugars. This example has been shown in the image below. The observation that there is a separate functional domain for each and every transformation required to produce a macrolide showed that for the modular PKSs, the programming of the PKS is achieved through a production-line approach. Figure 7

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    The various domains making up a polyketide synthase


    Component domains of polyketides consist of acyl-transferases (AT) for the loading of starter, extender and intermediate acyl units; acyl carrier proteins (ACP) which hold the growing macrolide as a thiol ester; b-keto-acyl synthases (KS) which catalyse chain extension; b-keto reductases (KR) responsible for the first reduction to an alochol functionality; dehydratases (DH)which eliminate water to give an unsaturated thiolester; enoyl reductases (ER) which catalyse the final reduction to full saturation; and finally a thiolesterase (TE) to catalyse macrolide release and cyclisation. Figure 8

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    The linker hypothesis


    Proposed first by Gokhale et al., (Science, 2000), this hypothesis states that linkers, which can be defined as the amino acid stretches joining various domains in a modular polyketide synthase, play a crucial role in the establishment of structural and functional assembly of these multimodular proteins. It is believed that these dynamic linkers establish communication by directing the correlated movements of various domains. Two categories of linkers have been postulated: linkers that connect covalently connected modules (intra-polypeptide linkers e.g, between modules 1 and 2 of DEBS) and linkers between modules that are present on two different polypeptides (inter-polypeptide linkers e.g., between modules 2 and 3 of DBES). The following figure explains the concept of linkers in a typical modular system: Figure 9

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    Scope of polyketide research...


    The enzymes that govern the assembly of polyketides by microorganisms, are receiving increasing attention as access to them has improved through molecular genetic methods. An explosion of discoveries and technological innovations has enhanced our capacity to "mutate" the structure of natural products using heuristics and procedures that are analogous to those routinely used in the generation of structurally altered nucleic acids and proteins. Some hurdles exist in the expansion of a biologist's synthetic capabilities but these barriers can be overcome by further research. The biological, biochemical, and medical implications would be immense, specially towards engineering artificial assemblies for production of novel biotherapeutics. Although organic chemists had conducted the primary research, geneticists and molecular biologists have completely transformed this field. Genetic manipulation techniques have resulted in the identification, cloning, sequencing and functional analyses of several polyketide biosynthetic pathways. Empirical gene fusion approaches have led to the biosynthesis of diverse unnatural natural products revealing the versatility and combinatorial potential of PKSs. With our advancement in the understanding of the molecular mechanistic basis of polyketide machinery, it should be possible to rationally alter the PKS genes. Figure 10 is an example of a successful inter-polypeptide chain transfer between fused modules of erythromycin and rifamycin polyketide synthases. Figure 10

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    ACKNOWLEDGEMENTS Excerpts(and/or Figures) taken from #Khosla C. et al. (1999) Annu. Rev. Biochem. 68:219-253 #Cox R. J. (2000) Annu. Rep. Prog. Chem., Sect. B, 96, 231-258 #Gokhale R. S and Tuteja D. (2001) Biotechnology, 10, 341-372 (Also Figures 1, 8 and 7) Figures 2 and 3 taken with thanks from Ms Yogyata (NII) Figure 10 from Gokhale R.S, Tsuji S.Y, Cane D.E and Khosla C. (1999) Science, Vol 284, 482-485 BACK TO TOP OF PAGE

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