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(DMB 01) M.Sc. (MICROBIOLOGY) (Previous) DEGREE EXAMINATION :: MAY 2006 Paper - I : INTRODUCTION TO MICRO ORGANISMS
Time: Three hours Maximum : 100 Marks

PART - A
Answer Any FIVE of the following: All questions carry equal marks.

(5 X 8 = 40 marks)

1. Controversy over Spontaneous Generation and Germ Theory of disease and fermentations. 2. Explain the contributions of Leeuwenhoek and Louis Pasteur to the development of Microbiology. 3. General characters of Archaeobacteria and Actinomycetes. 4. Staphylococcus and Escherichia in relation to human health. 5. Explain the morphology and chemistry of viruses. 6. Describe viroids and prions and their importance in nature. 7. Diversity of asexual reproduction in fungi. 8. Ainsworth system of classification of fungi. PART - B Answer all the FOUR Questions. UNIT - 1 (4 X 15 = 60 marks)

9. (a) Describe the microbial diversity and Carl Woese three domain system of classification. Or (b) Describe the ultra structure of a typical bacterial cell with illustrations and add a note on how it differs form that of an eukaryotic cell. UNIT II 10. (a) Write a critical account on the characteristics used in bacterial taxonomy. Or (b) Describe the latest classification of bacteria on Bergeys Manual of Systematic Bacteriology. UNIT III 11. (a) Describe the ultrastructure and replication of T4 phages with illustrations. Or (b) Discuss the symptoms of the diseases caused by viruses and methods of transmission of viruses. UNIT IV 12. (a) Discuss the structure, reproduction and economic importance of microalgae. Or (b) Describe the classification, structure and reproduction of protozoa and their ecological significance. ******

(DMB 02) M.Sc. (MICROBIOLOGY) (Previous) DEGREE EXAMINATION :: MAY 2006 Paper - II : MICROBIOLOGICAL METHODS
Time: Three hours Maximum : 100 Marks

PART - A
Answer Any FIVE of the following: All questions carry equal marks.

(5 X 8 = 40 marks)

1. Scanning Vs Transmission Electron Microscopy. 2. Principles involved and applications of dark field microscopy and fluorescent microscopy. 3. Plating methods and their applications. 4. Enrichment techniques and their applications. 5. Principles and applications of GLC and HPLC. 6. Isolation and purification of Viruses. 7. Principle involved and applications of two dimensional and pulse field gel electrophoresis. 8. Principle involved and applications of IR and Mass spectroscopy. SECTION - B (4 X 15 = 60 marks) Answer all the FOUR questions. UNIT I 9. (a) Discuss various methods used for staining of bacteria and their relative merits. Or (b) Discuss the methods of sterilization and the basic principles involved therein. UNIT II 10. (a) Explain the methods of anaerobic culturing and their applications. Or (b) Write a critical account on the methods of preservation of microbial cultures with a note on principles involved and relative merits. UNIT III 11. (a) Write a critical account on the differential and density gradient centrifugation techniques in the study of viruses. Or (b) Describe the methods commonly used in the cultivation of viruses with emphasis on the principles involved. UNIT IV 12. (a) Explain the application of radioactive substances in biology and the methods of detection and measurement of radioactivity. Or (b) What is Beer-Lambert Law? Explain the principles and applications of visible and UV-VIS spectrophotometric techniques in biology.

(DMB 03) M.Sc. (MICROBIOLOGY) (Previous) DEGREE EXAMINATION :: MAY 2006 Paper - III : MICROBIAL PHYSIOLOGY AND BIOCHEMISTRY
Time: Three hours Maximum : 100 Marks

PART - A
Answer Any FIVE of the following: All questions carry equal marks. 1. Measurement of bacterial growth. 2. Factors affecting bacterial growth. 3. Hydrogen and CO-oxidizers and their role in nature. 4. Oxygenic photosynthesis. 5. Different types of phosphorylations 6. Electron transport chain in bacteria and eukaryotes. 7. Properties of allosteric and isoenzymes. 8. Nature and classification of enzymes.

(5 X 8 = 40 marks)

SECTION - B (4 X 15 = 60 marks) Answer all the FOUR Questions. UNIT I 9. (a) Describe in detail bacterial growth and growth curve. Or (b) Discuss the nutritional requirements and nutrient transport in bacteria. UNIT II 10. (a) What is photoautotrophy? Describe the mechanism of photosynthesis in bacteria with illustrations. Or (b) What is chemoautotrophy? Discuss the significance of NO3, NH4 and methylotrophs in nature. UNIT III 11. (a) Describe the ethanol and lactate fermentations. Or (b) Describe nitrate reduction and methanogenesis in relation to their ecological significance. UNIT IV 12. (a) Write a critical account on the regulation of enzyme activity? Or (b) Describe the structure and functions of RNA and DNA. ******

(DMB 04) M.Sc. (MICROBIOLOGY) (Previous) DEGREE EXAMINATION :: MAY 2006 Paper - IV : ENVIRONMENTAL AND AGRICULTURAL MICROBIOLOGY
Time: Three hours Maximum : 100 Marks

PART - A
Answer Any FIVE of the following: All questions carry equal marks.

(5 X 8 = 40 marks)

1. Air sampling techniques 2. Coliform test for water quality. 3. Describe briefly the components of soil. 4. Methods of isolation of soil micro flora. 5. Plant growth promoting rhizobacteria. 6. Structure and function of legume root nodules. 7. Symptoms of diseases caused by bacteria and viruses in plants. 8. Symptomatology, epidemiology and control of late blight of potato and angular leaf spot of cotton. SECTION - B Answer all the FOUR questions. UNIT - I (4 X 15 = 60 marks)

9. (a) Discuss Aerobiology is relation to plant pathology and human health. Or (b) Describe the methods of treatment of sewage and the principles involved therein. UNIT - II 10. (a) Describe the components of soil organic matter and decompostion of carbonaceous materials in soil. Or (b) Discuss the microbial diversity in soil and factors affecting their abundance. UNIT - III 11. (a) Discuss the utilization of Azotobacter and Cyanobacteria as bio-inoculants. Or (b) Describe the nature of mycorrhizal associations and importance of VAM fungi in relation to nutrition of plants. UNIT - IV 12. (a) Explain the general principles of plant disease control with a special note on biological of plant diseases. Or (b) Describe the symptomatology, etiology, epidemiology and control of late blight of potato, black stem rust of wheat and soft rot of vegetables. ******

(DMB 21) M.Sc. (MICROBIOLOGY) (Final) DEGREE EXAMINATION :: MAY 2006 Paper - V :MEDICAL MICROBIOLOGY
Time: Three hours Maximum : 100 Marks

PART - A
Answer Any FIVE of the following: All questions carry equal marks. 1. Adhesins. 3. Treponema pallidum 5. Oncoviruses 7. Fluorescent microscopy

(5 X 8 = 40 marks)

2. Phagocytic cells 4. Aspergillosis 6. Amoebiosis - Extraintestinal 8. Types of epidemics. PART - B Answer ALL the four questions All questions carry equal marks (4 X 15 = 60 marks)

9. (a) Write on measurement of virulence and discuss in detail the invasive factors. Or (b) Enumerate barriers to infection and discuss in detail complement with special reference to its biological activities. 10. (a) Mention the etiological agents of bacterial diarrhoeas and discuss pathogenesis, epidemiology and laboratory diagnosis of vibrio cholerae. Or (b) Discuss deep mycoses in detail. Add a note on deep mycotic infections prevalent in India. 11. (a) Discuss pathogenesis, clinical features and laboratory diagnosis of HIV. Or (b) Write on morphology, life cycle, clinical features and laboratory diagnosis of plasmodium vivax. 12. (a) List out factors effecting epidemic outbreaks and discuss in detail disease reservoirs. Or (b) Discuss mechanisms of action of chemotherapeutic drugs and write on Penicillins. ******

(DMB 22) M.Sc. (MICROBIOLOGY) (Final) DEGREE EXAMINATION :: MAY 2006 Paper - VI :IMMUNOLOGY AND CELLULAR MICROBIOLOGY
Time: Three hours Maximum : 100 Marks

PART - A
Answer Any FIVE of the following: All questions carry equal marks. 1. Immunological memory 3. RIA. 5. Secretion systems in bacteria. 7. cAMP

(5 X 8 = 40 marks)

2. Immunogen 4. Atopy 6. Intracellular adhesion molecules. 8. Pore forming proteins (4 X 15 = 60 marks)

PART - B Answer ALL the four questions All questions carry equal marks

9. (a) Distinguish between humoral and cell mediated immune response. Or (b) What is a histocompatibility antigen? Describe the roles of the three HLA classes. 10. (a) What are the two types of enzyme immuno assay methods and explain their applications. Or (b) What are the types of evidence for establishing a disease as autoimmune disease and explain the rationate in using the monoclonal antibodies for therapy of various autoimmune diseases? 11. (a) Describe several specific adherence factors by which pathogens attach to host cells. Or (b) Describe the mode of action of some important gram-positive bacterial toxins. 12. (a) Differentiate between cell-to-cell and intracellular signalling pathways of bacteria. Or (b) Explain the role of eukaryotic cell-surface receptors in the signalling pathways with suitable examples. ******

(DMB 23) M.Sc. (MICROBIOLOGY) (Final) DEGREE EXAMINATION :: MAY 2006 Paper - VII :MICROBIAL GENETICS AND MOLECULAR BIOLOGY
Time: Three hours Maximum : 100 Marks

PART - A
Answer Any FIVE of the following: All questions carry equal marks. 1. Quorum sensing system 3. Chromosomal aberrations. 5. nif genes. 7. Tn3 transposan.

(5 X 8 = 40 marks)

2. Recombination frequency 4. Wobble hypothesis 6. Attenuated regulation 8. Transgenic plants PART - B (4 X 15 = 60 marks) Answer ALL the four questions All questions carry equal marks

9. (a) What are plasmids? Describe in detail features, types and significance. Or (b) Describe various genetic recombination occur in bacteria. 10. (a) Describe in detail various types of mutations and their molecular mechanisms with suitable examples. Or (b) Describe the molecular events in the initiation and termination of replication in bacteria. 11. (a) Compare the events involved in transcription for prokaryotic and eukaryotic mRNA synthesis. (b) Describe Jacob-Monod model for regulation of E. coli lac operon. 12. (a) Describe various tools and techniques used in molecular biology. Or (b) Write an essay on various cloning strategies from cloning gene to isolation of gene product. ******

(DMB 24) M.Sc. (MICROBIOLOGY) (Final) DEGREE EXAMINATION :: MAY 2006 Paper - VIII :FOOD AND INDUSTRIAL MICROBIOLOGY
Time: Three hours Maximum : 100 Marks

PART A
Answer Any FIVE of the following: All questions carry equal marks. 1. DEFT 3. Resuzurin test 5. Fermentation media for Glutamic acid production. 6. Sensors used in fermentor for monitoring and control. 7. Fermentor design for solid state fermentation. 8. Amylase production by fermentation.

(5 X 8 = 40 marks)

2. Radappertization 4. Vibriosis

PART - B Answer ALL the four questions All questions carry equal marks

(4 X 15 = 60 marks)

9. (a) Describe the microorganisms that are associated with foods and various sources of microbial contamination of foods? Or (b) From a given food sample, how do you isolate and enumerate the microorganisms? Describe various methods in detail. 10. (a) Describe the industrial preparation of Vinegar and its applications in food industry. Or (b) Describe in detail the cultivation of Mushrooms. 11. (a) Describe various components of fermentation media for commercially important microorganisms. Or (b) Describe strain improvement using mutagenesis and Recombinant DNA technology. 12. (a) Describe in detail the fermented media based for culturing of microorganisms for industrial production with suitable examples. Or (b) Describe various process involved in the extraction and cell disruption for various fermentation products, with suitable examples. ******