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    Estimation of PHENOLS

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    GajaNand Mallick
    This document describes a method for quantifying phenols using direct photometry. It involves distilling phenolic compounds from samples and allowing them to react with 4-aminoantipyrine and potassium ferric cyanide to form a colored dye. The absorbance of this dye is then measured at 500 nm and compared to standard phenol solutions to determine the concentration of phenols in the original sample. Precise reagents and buffers are listed to ensure accurate results and minimize interference from other compounds.

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    Estimation of PHENOLS

    Uploaded by

    GajaNand Mallick
    293 views2 pages
    This document describes a method for quantifying phenols using direct photometry. It involves distilling phenolic compounds from samples and allowing them to react with 4-aminoantipyrine and potassium ferric cyanide to form a colored dye. The absorbance of this dye is then measured at 500 nm and compared to standard phenol solutions to determine the concentration of phenols in the original sample. Precise reagents and buffers are listed to ensure accurate results and minimize interference from other compounds.

    Original Description:

    estimation of phenols in water

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    This document describes a method for quantifying phenols using direct photometry. It involves distilling phenolic compounds from samples and allowing them to react with 4-aminoantipyrine and potassium ferric cyanide to form a colored dye. The absorbance of this dye is then measured at 500 nm and compared to standard phenol solutions to determine the concentration of phenols in the original sample. Precise reagents and buffers are listed to ensure accurate results and minimize interference from other compounds.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    293 views2 pages

    Estimation of PHENOLS

    Uploaded by

    GajaNand Mallick
    This document describes a method for quantifying phenols using direct photometry. It involves distilling phenolic compounds from samples and allowing them to react with 4-aminoantipyrine and potassium ferric cyanide to form a colored dye. The absorbance of this dye is then measured at 500 nm and compared to standard phenol solutions to determine the concentration of phenols in the original sample. Precise reagents and buffers are listed to ensure accurate results and minimize interference from other compounds.

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    PHENOLS

    Method - Direct Photometric



    Principle

    Steam- distillable phenolic compounds react with 4 aminoantipyrine at pH 7.9 0.1 in the
    presence of potassium ferric cyanide to form a colored antipyrine dye. This dye is kept in
    aqueous solution and the absorbance is measured at 500 nm.

    Interference

    Interference are eliminated or reduced to minimum by using the distillate from the
    preliminary distillation procedure.

    Reagents

    (1) Stock Phenol Solution: - Dissolve 100-mg phenol in freshly boiled and cooled
    distilled water and dilute to 100 ml.

    (2) Intermediate Phenol Solution: - Dilute 1.0-ml stock phenol solution in freshly
    boiled and cooled distilled water to 100 ml.

    (3) Standard Phenol Solution: - Dilute 50.0-ml intermediate phenol solution to 500 ml
    with freshly boiled and cooled distilled water.
    1 ml = 1.0 g phenol Prepare within 2 hrs. of use

    (4) Bromate-bromide Solution: - Dissolve 2.784 gm an hydrous KbrO3 in water, add 10
    gm Kbr Crystals, dissolve, and dilute to 1000 ml.
    (5) Hydrochloric Acid (HCL) :- Concentrated

    (6) Standard Sodium Thiosulphate Titrant) 0.025 M): - Dissolve 6.205 gm Na2S2O3
    5H2O in distilled water. Add 1.5 ml 6 N NaOH or 0.4 gm solid NaOH and dilute to
    1000 ml. Standardize with bi-iodate solution.
    (7) Starch Solution: - Use either an aqueous solution or soluble starch powder mixtures.

    To prepare an aqueous solution, dissolve 2 gm laboratory grade soluble starch and
    0.2 gm salicylic acid, as a preservative, in 100 ml hot distilled water.

    (8) Ammonium Hydroxide (NH4OH 0.5): - Dilute 35 fresh concentrate NH4OH to 1 ltr.
    with distilled water.

    (9) Phosphate Buffer Solution: - Dissolve 104.5 gm K2HPO4 and 72.3 gm KH2 PO4 in
    water and dilute to 1 ltr. The pH should be 6.8.

    (10) 4-Aminoantipyrine Solution; - Dissolve 2.0 gm 4 aminoantipyrine in water and
    dilute to 100 ml. Prepare daily.

    (11) Potassium Ferricyanide Solution: - Dissolve 8.0 gm K3 Fe (CN) 6 in water and
    dilute to 100 ml. Filter if necessary. Store in a brown glass bottle. Prepare fresh
    weekly.

    (12) Chloroform (CHCl3)

    (13) Sodium Sulphate: - Anhydrous Na2SO4, granules

    (14) Potassium Iodide: - K I, Crystals.
    Procedure

    Place 100-ml distillate or a portion containing not more than 0.5-m phenol diluted to 100 ml,
    in a 250-ml beaker. Prepare 100-ml. Distilled water blank and a series of 100 ml. Phenol
    standards containing 0.1, 0.2,0.3, 0.4 and 0.5 mg phenol. Treat sample, blank and standards
    as follows: Add 2.5 ml. 0.5 N NH4OH solution and immediately adjust to pH 7.9 0.1 with
    phosphate buffer. Add 1.0 ml 4-aminoantipyrine solution, mix well, add 1.0 ml K3Fe (CN)6
    solution, and mix well. After 15 min. transfer to cells and read absorbance of sample and
    standards against at 500 nm.
    Calculation

    Mg phenol/L = A x 1000 / B

    Where,

    A = mg phenol in sample, from calibration curve and
    B = ml original sample

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