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    Cellular Molecular Biology

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    This document provides details about a Cellular and Molecular Biology course taken during the first year of a Medicine program. The course is compulsory and carries 5 credits. It includes 24 hours of lectures and 28 hours of laboratory work over the semester. The lectures cover topics like cell structure and function, organelles, membrane transport, cytoskeleton, cell cycle, and apoptosis. The laboratory schedule includes techniques like microscopy, staining, and examining cellular morphology. The final grade is based on tests, exams, and lab/lecture assessments.

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    Cellular Molecular Biology

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    Cpop
    154 views4 pages
    This document provides details about a Cellular and Molecular Biology course taken during the first year of a Medicine program. The course is compulsory and carries 5 credits. It includes 24 hours of lectures and 28 hours of laboratory work over the semester. The lectures cover topics like cell structure and function, organelles, membrane transport, cytoskeleton, cell cycle, and apoptosis. The laboratory schedule includes techniques like microscopy, staining, and examining cellular morphology. The final grade is based on tests, exams, and lab/lecture assessments.

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    This document provides details about a Cellular and Molecular Biology course taken during the first year of a Medicine program. The course is compulsory and carries 5 credits. It includes 24 hours of lectures and 28 hours of laboratory work over the semester. The lectures cover topics like cell structure and function, organelles, membrane transport, cytoskeleton, cell cycle, and apoptosis. The laboratory schedule includes techniques like microscopy, staining, and examining cellular morphology. The final grade is based on tests, exams, and lab/lecture assessments.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    154 views4 pages

    Cellular Molecular Biology

    Uploaded by

    Cpop
    This document provides details about a Cellular and Molecular Biology course taken during the first year of a Medicine program. The course is compulsory and carries 5 credits. It includes 24 hours of lectures and 28 hours of laboratory work over the semester. The lectures cover topics like cell structure and function, organelles, membrane transport, cytoskeleton, cell cycle, and apoptosis. The laboratory schedule includes techniques like microscopy, staining, and examining cellular morphology. The final grade is based on tests, exams, and lab/lecture assessments.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4
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    The key takeaways from this document are that it provides an overview of a Cellular and Molecular Biology course, including its objectives, lecture and laboratory content, assessment methods, and time allocation for individual study.

    The main topics covered in the lectures include cell structure and organelles, membrane structure and functions, cytoskeleton, cell adhesion, cell cycle, apoptosis, and molecular biology of tumor cells.

    The laboratory sessions cover techniques such as light and electron microscopy, cell culture, electrophoresis, staining, and methods for studying cell division, viability, and morphology.

    DISCIPLINE RECORD

    Total hours (per semester) of


    curriculum (52 )
    Discipline Title

    CELLULAR AND MOLECULAR BIOLOGY

    Year of study

    Semester *

    The type of final assessment (E / V /


    C)

    E1

    Discipline conditions (Ob compulsory, Op optional, F - facultative)

    Ob

    Number of credits

    Total hours of individual


    study

    75

    Total hours per


    semester

    52

    Total hours of curriculum


    Discipline coordinator

    52

    Prof. Univ. Dr. Carmen Elena Cotrutz

    Faculty

    Medicine

    Department

    Morpho-functional Sciences

    Profile

    Medicine

    Specialization

    Medicine

    Total
    52

    C**

    24

    28

    Purposes
    1. understanding cell and subcellular universe regarding human body
    2. knowledges regarding the structure and function for cell and substructres
    3. basic knowledge regarding pathology involvment for cell structures and their main functions
    Lecture content 24 hrs
    1. Introduction to cell and molecular biology. 1h
    Definitions. Study object. Importance for medicine. General organization of the cells: prokaryotes, eukaryotes.
    2. Cell cover. 2h
    Cell membrane classification, membrane model; molecular structure of cell membrane; molecular asymmetry for
    cell membranes, glycocalyx
    3. Cell membrane functions (I). 1h
    Molecular biology of cell membrane transport general aspects, pathological aspects
    4. Cell membrane functions (II). 1h
    vesicular transport (exocytosis, endocytosis, transcytosis) LD internalization, pathological aspects
    5. Cell membrane functions (III) 1h
    intercellular signaling and information interchange (ways of intercellular signaling, signal molecules, paracrin
    signaling, endocrine signaling, neurocrin signaling, autocrine signaling, cell receptors)
    6. Cell membrane functions (IV) 2h

    - information exchange via polypeptide and steroid hormones


    7. Cytoskeleton, the molecular support for cell motility: 2h
    classification, general structure, actin and associated molecules: actin-myosin interaction. Microtubules.
    Intermediate filaments, cytoskeleton functions
    8. Cell membrane specializations at apical and basal pole. 1h
    Morphological , molecular and pathological aspects for microvillia, cilia and stereocilia.
    9. Cell adhesion and extracellular matrix. 2h
    Junctional proteins, cell-cell and cell-matrix interactions. Cell junctions in pathology. Extracellular matrix and its
    functions in tissue organization.
    10. Nucleus in interphase. 1h
    General aspects, molecular structure. Nuclear membrane molecular structure. Chromatin and nucleolus structure.
    Interchromatin space
    11. Cellular synthesis and secretion, 1h
    cellular synthesis and secretion vesicles traffic
    12. Cellular synthesis and secretion organelles (I), ribosomes and proteogenesis 1h
    13. Cellular synthesis and secretion organelles (II), endoplasmic reticulum and Golgi apparatus 1h
    14. Energy generating organelle: mitochondria and molecular biology of respiratory chain.
    15. Cellular digestion organelles: lisosomes and peroxisomes; implications in pathology 1h
    16. Cell cycle: generalities regarding cell cycle, cell cycle horologe control. Interphase, mitotic cell division.
    Reductional cell division (mitosis) 2h
    17. Cell senescence: general presentation; cell senescence and general aging of the organisms; morphologic and
    molecular characteristics for senescent cells; theories regarding cellular aging. 1h
    18. Apoptosis: 1h
    general presentation; programmed cell death concept. Apoptosis issuing circumstances; Molecular mechanisms
    of apoptosis. Morphological and molecular characteristics of apoptotic cells.
    19. Molecular biology of the tumor cell 1h

    Lab content Schedule 28 hrs


    1. Imaging techniques in cell and molecular biology: the light microscope,
    fluorescence microscopy, phase contrast microscopy, polarized light microscopy
    2. Electron microscopy (I) classification. Transmission electron microscope TEM,
    SEM and other electron microscopes
    3. Electron microscopy (II) methods for tissue processing regarding examination in TEM
    4. Special techniques in cell and molecular biology cell cultures, cryofracture,
    ultracentrifugation and cell fractioning, chromatography electrophoresis and DNA separation,
    X-ray diffraction.
    5. Fine sectioning method (prelevation, fixation, embedding materials, sectioning,
    section spreading, slide mounting)
    6. Ice sectioning, criotome (technique, indications).
    7. Staining and their mechanisms: paraffin and ice sections staining; vital staining of
    cells and organelles
    8. Smear technique: blood smear CBC, WBC count, blood cells morphology and
    staining
    9. Citochemistry and IHC: glycogen leukocyte, peroxidase, nucleic acids evidentiation
    10. Cellular and nuclear morphology in light and electronic microscopy: cell and
    nucleus shape, size, number
    11. Cell membrane specialisations aspects in light and electron microscopy
    12. Cell organelles for synthesis and secretion, digestion and energy generation:
    aspects in light and electron microscopy
    13. Methods for cell division study. Cell division in light and electron microscopy
    14 Cell and molecular biology methods for identification and quantification of cell
    viability.
    2 hours each lab
    References
    1. Alberts B., et all - Molecular Biology of the Cell, 5th Edition, 2009
    2. Cotrutz C., Cotrutz C.E. - Cell and Molecular Biology, Course, 1997
    3. Steven R. Goodman Medical Cell Biology, Second Edition, 2000
    4. Gerald Karp - Cell and Molecular Biology, Third Edition, 2002
    Qualifications
    1. Basic cell and molecular techniques for cell study
    2. Microscopy techniques - light and electron microscopy, biologic sample preparation
    3. General aspects regardig cell and its organelles in light and electron microscopy
    4. Performing and staining ice and paraffin thin sections
    5. Blood smear preparation and interpretation
    Percent
    The final mark is formed by
    (Total = 100%)
    - answers from semestrial MCQ lecture test:

    5%

    - answers from semestrial MCQ practical test


    - average from weekly tests
    - final practical exam

    5%

    5%
    35%

    - Final lecture MCQ


    50%
    EVALUATION DETAILS
    1. Tests during semester
    MCQ from lectures - 20 questions
    Practical/oral test for lab subjects
    2. Final exam formed by:
    - practical examination 3 questions from lab schedule
    - MCQ test (50 questions simple choice from the lectures, duration 1h15 min)
    Total time (hours per semester) for individual study
    15
    1. Understanding lecture notes
    8. Preparing oral presentation
    2. Study acording to lecture support

    30

    9. Preparing the final exam

    30

    3. Study according to references

    10

    10. Counselling

    4. Study in the library

    11. On place documentation

    5. Preparing the lab/seminary

    12. Internet study

    10

    6. Homeworks

    15

    13. Other ....

    7. Preparing for tests

    15

    14. Other

    0
    130

    TOTAL hours individual study (per


    semester) = minimum
    Teaching methods:
    1. Interactive problem based lectures baed on rich content multimedia prsentations
    2. Labs are developed in workgroups that compare and evaluate experimental data
    obtained following guided and supervised labwork
    Data completrii:
    2012
    Prof. Univ. Dr. Carmen Elena Cotrutz

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